Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/149225 |
Resumo: | Calcium silicate-based cements are studied as reparative materials. This study was divided into 4 chapters: In the first, cytotoxicity (MTT and apoptosis) and genotoxicity (Comet assay) were evaluated in Saos-2 for: Pure calcium silicatebased (CSC); Modified (CSCM); resin-based (CSCR1, CSCR2, CSCR3). In the Viability assay, CSC and CSCR3 (50mg/mL) showed lower cell viability. CSCR1, CSCR2, CSCR3 showed more apoptosis. Only CSC and CSCR2 were not genotoxity in 10mg/mL (P<0.05). Chapter 2, CSCM and CSCR2 were associated with radiopacifiers: zirconium oxide and niobium oxide (micro and nano), bismuth oxide and calcium tungstate. MTA was used for the control of cytotoxicity and bioactivity tests. All were viable and showed similar apoptosis (1:8). Necrosis was superior (P<0.05). CSCM and CSCR induced alkaline phosphatase (ALP) and ARS. Chapter 3, was compared Biodentine (Septodont), MTA Plus (Avalon), CSCRs ZrO2 and Nb2O5, on cytotoxicity and genotoxicity. In MTT (1, 3 and 7 days) all were similar. In the qPCR, BAX was expressed by CSCRs (3d). MTAP and CSCR ZrO2 expressed in 5 days. For BCL2 gene (3 and 5d) only MTAP and CSCR Nb2O5 (5d). In genotoxixity assay, all (1:2 and 1:8) were similar (P<0.05). Chapter 4, we evaluated the same materials in Saos2 bioactivity: MTT, cell proliferation, ALP (1, 3 and 7d), qPCR (alp and ocn) and ARS. All groups were viable and induced ALP, ARS and gene expression, particularly CSCR Nb2O5 and Biodentine. Therefore, the biological materials has the potential to be used in endodontics. Additional studies should be conducted, especially for experimental cements. Additional studies should be conducted, especially for experimental cements. |
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Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA PlusCytotoxicity, genotoxicity and bioactivity of experimental calcium silicate based cements with different radiopacifiers, Biodentine and MTA Plus sealersToxicologia genéticaCelulas - Cultura e meios de culturaEndodontiaEndodonticsMaterials testingCalcium silicate-based cements are studied as reparative materials. This study was divided into 4 chapters: In the first, cytotoxicity (MTT and apoptosis) and genotoxicity (Comet assay) were evaluated in Saos-2 for: Pure calcium silicatebased (CSC); Modified (CSCM); resin-based (CSCR1, CSCR2, CSCR3). In the Viability assay, CSC and CSCR3 (50mg/mL) showed lower cell viability. CSCR1, CSCR2, CSCR3 showed more apoptosis. Only CSC and CSCR2 were not genotoxity in 10mg/mL (P<0.05). Chapter 2, CSCM and CSCR2 were associated with radiopacifiers: zirconium oxide and niobium oxide (micro and nano), bismuth oxide and calcium tungstate. MTA was used for the control of cytotoxicity and bioactivity tests. All were viable and showed similar apoptosis (1:8). Necrosis was superior (P<0.05). CSCM and CSCR induced alkaline phosphatase (ALP) and ARS. Chapter 3, was compared Biodentine (Septodont), MTA Plus (Avalon), CSCRs ZrO2 and Nb2O5, on cytotoxicity and genotoxicity. In MTT (1, 3 and 7 days) all were similar. In the qPCR, BAX was expressed by CSCRs (3d). MTAP and CSCR ZrO2 expressed in 5 days. For BCL2 gene (3 and 5d) only MTAP and CSCR Nb2O5 (5d). In genotoxixity assay, all (1:2 and 1:8) were similar (P<0.05). Chapter 4, we evaluated the same materials in Saos2 bioactivity: MTT, cell proliferation, ALP (1, 3 and 7d), qPCR (alp and ocn) and ARS. All groups were viable and induced ALP, ARS and gene expression, particularly CSCR Nb2O5 and Biodentine. Therefore, the biological materials has the potential to be used in endodontics. Additional studies should be conducted, especially for experimental cements. Additional studies should be conducted, especially for experimental cements.Cimentos de silicato decálciosão estudados como materiais reparadores. O estudo foi divido em 4 capítulos: No primeiro, citotoxicidade (MTT e Apoptose), genotoxicidade (teste Cometa) foram avaliadas em Saos-2 para os materiais: Cimentos de silicato de cálcio puro (CSC); Modificado (CSCM); Resinoso (CSCR1, CSCR2 e CSCR3). Na viabilidade, CSC e CSCR3 (50mg/mL) foram citotóxicos. CSCR1, CSCR2 e CSCR3 mostraram maior apoptose. Somente CSC e CSCR2 não foram genotóxicos em 10mg/mL (P<0.05). No cap.2, CSCM e CSCR2, foram associados a radiopacificadores: óxido de zircônio e óxido de nióbio (micro e nano), óxido de bismuto, tungstato de cálcio. MTA foi o controle para citotoxicidade e bioatividade. Todos foram viáveis e apresentaram apoptose semelhantes (1:8). A necrose foi superior (P<0.05). Ambos CSCs induziram fosfatase alcalina (ALP) e ARS. No cap.3, Biodentine (Septodont), MTA Plus (Avalon), CSCRs Nb2O5 e ZrO2 foram analisados quanto à cito e genotoxicidade. No MTT (1, 3 e 7d), todos foram similares. No qPCR, houve expressão de BAX (3d.) para CSCRs, MTAP e CSCR ZrO2 (5d). Para BCL2, (3 e 5d) somente MTAP e CSCR Nb2O5 (5d.). Na genotoxicidade, todos (1:2 e 1:8) permaneceram similares (P<0.05). Cap. 4, os mesmos foram avaliados na bioatividade: MTT, proliferação celular, ALP (1, 3 e 7d), qPCR (alp e ocn), e ARS. Todos os grupos foram viáveis e induziram ALP, ARS e expressão gênica, destacando os materiais CSCR Nb2O5 e Biodentine. Desta forma, os materiais apresentam potencial biológico para ser usado na endodontia. Estudos adicionais devem ser realizados, especialmente para os materiais experimentais.Universidade Estadual Paulista (Unesp)Tanomaro Filho, Mario [UNESP]Universidade Estadual Paulista (Unesp)Cornélio, Ana Lívia Gomes [UNESP]2017-03-14T14:10:06Z2017-03-14T14:10:06Z2015-03-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis99 f.application/pdfCORNÉLIO, Ana Lívia Gomes. Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus. 2015. 99 f. Tese (Doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia (FOAR), 2015.http://hdl.handle.net/11449/149225000874521000874521.pdf33004030059P1Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-01-10T06:22:53Zoai:repositorio.unesp.br:11449/149225Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:34:33.953645Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus Cytotoxicity, genotoxicity and bioactivity of experimental calcium silicate based cements with different radiopacifiers, Biodentine and MTA Plus sealers |
title |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus |
spellingShingle |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus Cornélio, Ana Lívia Gomes [UNESP] Toxicologia genética Celulas - Cultura e meios de cultura Endodontia Endodontics Materials testing |
title_short |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus |
title_full |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus |
title_fullStr |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus |
title_full_unstemmed |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus |
title_sort |
Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus |
author |
Cornélio, Ana Lívia Gomes [UNESP] |
author_facet |
Cornélio, Ana Lívia Gomes [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Tanomaro Filho, Mario [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cornélio, Ana Lívia Gomes [UNESP] |
dc.subject.por.fl_str_mv |
Toxicologia genética Celulas - Cultura e meios de cultura Endodontia Endodontics Materials testing |
topic |
Toxicologia genética Celulas - Cultura e meios de cultura Endodontia Endodontics Materials testing |
description |
Calcium silicate-based cements are studied as reparative materials. This study was divided into 4 chapters: In the first, cytotoxicity (MTT and apoptosis) and genotoxicity (Comet assay) were evaluated in Saos-2 for: Pure calcium silicatebased (CSC); Modified (CSCM); resin-based (CSCR1, CSCR2, CSCR3). In the Viability assay, CSC and CSCR3 (50mg/mL) showed lower cell viability. CSCR1, CSCR2, CSCR3 showed more apoptosis. Only CSC and CSCR2 were not genotoxity in 10mg/mL (P<0.05). Chapter 2, CSCM and CSCR2 were associated with radiopacifiers: zirconium oxide and niobium oxide (micro and nano), bismuth oxide and calcium tungstate. MTA was used for the control of cytotoxicity and bioactivity tests. All were viable and showed similar apoptosis (1:8). Necrosis was superior (P<0.05). CSCM and CSCR induced alkaline phosphatase (ALP) and ARS. Chapter 3, was compared Biodentine (Septodont), MTA Plus (Avalon), CSCRs ZrO2 and Nb2O5, on cytotoxicity and genotoxicity. In MTT (1, 3 and 7 days) all were similar. In the qPCR, BAX was expressed by CSCRs (3d). MTAP and CSCR ZrO2 expressed in 5 days. For BCL2 gene (3 and 5d) only MTAP and CSCR Nb2O5 (5d). In genotoxixity assay, all (1:2 and 1:8) were similar (P<0.05). Chapter 4, we evaluated the same materials in Saos2 bioactivity: MTT, cell proliferation, ALP (1, 3 and 7d), qPCR (alp and ocn) and ARS. All groups were viable and induced ALP, ARS and gene expression, particularly CSCR Nb2O5 and Biodentine. Therefore, the biological materials has the potential to be used in endodontics. Additional studies should be conducted, especially for experimental cements. Additional studies should be conducted, especially for experimental cements. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-30 2017-03-14T14:10:06Z 2017-03-14T14:10:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CORNÉLIO, Ana Lívia Gomes. Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus. 2015. 99 f. Tese (Doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia (FOAR), 2015. http://hdl.handle.net/11449/149225 000874521 000874521.pdf 33004030059P1 |
identifier_str_mv |
CORNÉLIO, Ana Lívia Gomes. Avaliação da citotoxicidade, genotoxicidade e bioatividade de cimentos experimentais a base de silicato de cálcio com diferentes radiopacificadores e dos cimentos Biodentine e MTA Plus. 2015. 99 f. Tese (Doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia (FOAR), 2015. 000874521 000874521.pdf 33004030059P1 |
url |
http://hdl.handle.net/11449/149225 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
99 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129440080723968 |