Toothpick test: a methodology for the detection of RR soybean plants
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?pid=S1806-66902015000200436&script=sci_arttext&tlng=pt http://hdl.handle.net/11449/129539 |
Resumo: | Due to the large increase in the area cultivated with genetically modified soybean in Brazil, it has become necessary to determine methods that are fast and efficient for detecting these cultivars. The aim of this work was to test the efficiency of the toothpick method in the detection of RR soybean plants, as well as to distinguish between cultivars, for sensitivity caused by herbicide. Ten transgenic soybean cultivars, resistant to the active ingredient glyphosate, and ten conventional soybean cultivars were used. Toothpicks soaked in glyphosate were applied to all the plants at stage V6 and evaluations were made at 2, 4, 6, 8 and 10 days after application (DAA). The effects of the glyphosate on the cultivars, and the symptoms of phytotoxicity caused in the transgenic plants were evaluated by means of grading scales. The toothpick test is effective in identifying RR soybean cultivars and also in separating them into groups by sensitivity to the symptoms caused by the glyphosate. |
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Toothpick test: a methodology for the detection of RR soybean plantsTeste do palito: metodologia para detecção de plantas de soja RRGlycine maxPhytotoxicityGlyphosateToothpick methodHerbicideGlycine maxFitotoxidadeGlifosatoMétodo do palitoHerbicidaDue to the large increase in the area cultivated with genetically modified soybean in Brazil, it has become necessary to determine methods that are fast and efficient for detecting these cultivars. The aim of this work was to test the efficiency of the toothpick method in the detection of RR soybean plants, as well as to distinguish between cultivars, for sensitivity caused by herbicide. Ten transgenic soybean cultivars, resistant to the active ingredient glyphosate, and ten conventional soybean cultivars were used. Toothpicks soaked in glyphosate were applied to all the plants at stage V6 and evaluations were made at 2, 4, 6, 8 and 10 days after application (DAA). The effects of the glyphosate on the cultivars, and the symptoms of phytotoxicity caused in the transgenic plants were evaluated by means of grading scales. The toothpick test is effective in identifying RR soybean cultivars and also in separating them into groups by sensitivity to the symptoms caused by the glyphosate.Devido ao grande aumento da área cultivada com soja geneticamente modificada no Brasil, faz-se necessária a determinação de métodos que sejam rápidos e eficientes na detecção dessas cultivares. O objetivo deste trabalho foi testar a eficiência do método do palito na detecção de plantas de soja RR, bem como distinguir as cultivares quanto à sensibilidade causada pelo herbicida. Foram utilizadas dez cultivares de soja transgênica, resistentes ao ingrediente ativo glifosato, e dez cultivares de soja convencional. Palitos de dente embebidos em glifosato foram aplicados em todas as plantas em estádio V6 e as avaliações foram realizadas aos 2; 4; 6; 8 e 10 dias após a aplicação (DAA). Os efeitos da ação do glifosato nas cultivares e os sintomas de fitotoxicidade, ocasionados nas plantas transgênicas, foram avaliados por meio de escalas de notas. O teste do palito é eficiente para identificar as cultivares de soja RR e, além disso, separálas em grupos de sensibilidade aos sintomas causados pelo glifosato.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Prod Vegetal, Melhoramento Genet Plantas, Sao Paulo, SP, BrazilUniv Estadual Paulista, Estat Expt, Dept Ciencias Exatas, Sao Paulo, SP, BrazilUniv Estadual Paulista, Dept Prod Vegetal, Melhoramento Genet Plantas, Sao Paulo, SP, BrazilUniv Estadual Paulista, Estat Expt, Dept Ciencias Exatas, Sao Paulo, SP, BrazilUniv Federal Ceara, Dept GeolUniversidade Estadual Paulista (Unesp)Silva, Fabiana Mota da [UNESP]Uneda-Trevisoli, Sandra Helena [UNESP]Villela, Otavia Tiago [UNESP]Perecin, Dilermando [UNESP]Di Mauro, Antonio Orlando [UNESP]2015-10-21T21:18:57Z2015-10-21T21:18:57Z2015-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article436-442application/pdfhttp://www.scielo.br/scielo.php?pid=S1806-66902015000200436&script=sci_arttext&tlng=ptRevista Ciencia Agronomica. Fortaleza: Univ Federal Ceara, Dept Geol, v. 46, n. 2, p. 436-442, 2015.1806-6690http://hdl.handle.net/11449/12953910.5935/1806-6690.20150024S1806-66902015000200436WOS:000352272800024S1806-66902015000200436.pdf70873728847265591275652518822095Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Ciencia Agronomica0.6050,498info:eu-repo/semantics/openAccess2023-12-05T06:14:32Zoai:repositorio.unesp.br:11449/129539Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-05T06:14:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toothpick test: a methodology for the detection of RR soybean plants Teste do palito: metodologia para detecção de plantas de soja RR |
title |
Toothpick test: a methodology for the detection of RR soybean plants |
spellingShingle |
Toothpick test: a methodology for the detection of RR soybean plants Silva, Fabiana Mota da [UNESP] Glycine max Phytotoxicity Glyphosate Toothpick method Herbicide Glycine max Fitotoxidade Glifosato Método do palito Herbicida |
title_short |
Toothpick test: a methodology for the detection of RR soybean plants |
title_full |
Toothpick test: a methodology for the detection of RR soybean plants |
title_fullStr |
Toothpick test: a methodology for the detection of RR soybean plants |
title_full_unstemmed |
Toothpick test: a methodology for the detection of RR soybean plants |
title_sort |
Toothpick test: a methodology for the detection of RR soybean plants |
author |
Silva, Fabiana Mota da [UNESP] |
author_facet |
Silva, Fabiana Mota da [UNESP] Uneda-Trevisoli, Sandra Helena [UNESP] Villela, Otavia Tiago [UNESP] Perecin, Dilermando [UNESP] Di Mauro, Antonio Orlando [UNESP] |
author_role |
author |
author2 |
Uneda-Trevisoli, Sandra Helena [UNESP] Villela, Otavia Tiago [UNESP] Perecin, Dilermando [UNESP] Di Mauro, Antonio Orlando [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Fabiana Mota da [UNESP] Uneda-Trevisoli, Sandra Helena [UNESP] Villela, Otavia Tiago [UNESP] Perecin, Dilermando [UNESP] Di Mauro, Antonio Orlando [UNESP] |
dc.subject.por.fl_str_mv |
Glycine max Phytotoxicity Glyphosate Toothpick method Herbicide Glycine max Fitotoxidade Glifosato Método do palito Herbicida |
topic |
Glycine max Phytotoxicity Glyphosate Toothpick method Herbicide Glycine max Fitotoxidade Glifosato Método do palito Herbicida |
description |
Due to the large increase in the area cultivated with genetically modified soybean in Brazil, it has become necessary to determine methods that are fast and efficient for detecting these cultivars. The aim of this work was to test the efficiency of the toothpick method in the detection of RR soybean plants, as well as to distinguish between cultivars, for sensitivity caused by herbicide. Ten transgenic soybean cultivars, resistant to the active ingredient glyphosate, and ten conventional soybean cultivars were used. Toothpicks soaked in glyphosate were applied to all the plants at stage V6 and evaluations were made at 2, 4, 6, 8 and 10 days after application (DAA). The effects of the glyphosate on the cultivars, and the symptoms of phytotoxicity caused in the transgenic plants were evaluated by means of grading scales. The toothpick test is effective in identifying RR soybean cultivars and also in separating them into groups by sensitivity to the symptoms caused by the glyphosate. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T21:18:57Z 2015-10-21T21:18:57Z 2015-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?pid=S1806-66902015000200436&script=sci_arttext&tlng=pt Revista Ciencia Agronomica. Fortaleza: Univ Federal Ceara, Dept Geol, v. 46, n. 2, p. 436-442, 2015. 1806-6690 http://hdl.handle.net/11449/129539 10.5935/1806-6690.20150024 S1806-66902015000200436 WOS:000352272800024 S1806-66902015000200436.pdf 7087372884726559 1275652518822095 |
url |
http://www.scielo.br/scielo.php?pid=S1806-66902015000200436&script=sci_arttext&tlng=pt http://hdl.handle.net/11449/129539 |
identifier_str_mv |
Revista Ciencia Agronomica. Fortaleza: Univ Federal Ceara, Dept Geol, v. 46, n. 2, p. 436-442, 2015. 1806-6690 10.5935/1806-6690.20150024 S1806-66902015000200436 WOS:000352272800024 S1806-66902015000200436.pdf 7087372884726559 1275652518822095 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Ciencia Agronomica 0.605 0,498 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
436-442 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Federal Ceara, Dept Geol |
publisher.none.fl_str_mv |
Univ Federal Ceara, Dept Geol |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965174833086464 |