Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acs.jproteome.0c01044 http://hdl.handle.net/11449/207546 |
Resumo: | Infertility caused by male factors is potentially associated with metabolic disorders such as obesity and/or diabetes. This experimental study was conducted in a male rodent model to assess the effects of different diseases on semen quality and sperm proteomics. Ten Wistar rats were used for each treatment. Rats were fed commercial food provided controllably to the control group and the diabetic group, and a hypercaloric diet supplemented with 5% sucrose in water was provided ad libitum to the obese group for 38 weeks. Diabetes was induced with 35 mg/kg streptozotocin. After euthanasia, testicles, spermatozoa, fat, and blood (serum) samples were collected. Spermatozoa were evaluated for quality and subjected to proteomics analyses. Histology and cytology of the testis, and serum leptin, adiponectin, interleukin 8 (IL-8), blood glucose, and testosterone levels, were also assessed. Body weight, retroperitoneal and testicular fat, and the Lee index were also measured. Obesity and diabetes were induced. The diabetic group showed noticeable changes in spermatogenesis and sperm quality. The mass spectrometry proteomics data have been deposited in Mendeley Data (doi: 10.17632/rfp7kfjcsd.5). Fifteen proteins varied in abundance between groups, especially proteins related to energy production and structural function of the spermatozoa, suggesting disturbances in energy production with a subsequent alteration in sperm motility in both groups, but with a compensatory response in the obese group. |
id |
UNSP_fe0d56753ce8cc8b57d5768ec32ec3f4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/207546 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Ratsdiabeticobeseratsperm metabolismsperm proteinspermatogenesisInfertility caused by male factors is potentially associated with metabolic disorders such as obesity and/or diabetes. This experimental study was conducted in a male rodent model to assess the effects of different diseases on semen quality and sperm proteomics. Ten Wistar rats were used for each treatment. Rats were fed commercial food provided controllably to the control group and the diabetic group, and a hypercaloric diet supplemented with 5% sucrose in water was provided ad libitum to the obese group for 38 weeks. Diabetes was induced with 35 mg/kg streptozotocin. After euthanasia, testicles, spermatozoa, fat, and blood (serum) samples were collected. Spermatozoa were evaluated for quality and subjected to proteomics analyses. Histology and cytology of the testis, and serum leptin, adiponectin, interleukin 8 (IL-8), blood glucose, and testosterone levels, were also assessed. Body weight, retroperitoneal and testicular fat, and the Lee index were also measured. Obesity and diabetes were induced. The diabetic group showed noticeable changes in spermatogenesis and sperm quality. The mass spectrometry proteomics data have been deposited in Mendeley Data (doi: 10.17632/rfp7kfjcsd.5). Fifteen proteins varied in abundance between groups, especially proteins related to energy production and structural function of the spermatozoa, suggesting disturbances in energy production with a subsequent alteration in sperm motility in both groups, but with a compensatory response in the obese group.Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University júlio de Mesquita Filho-UNESP, Rua Prof. Dr. Walter Maurício Correa, s/n, Rubião JuniorDepartment of Veterinary Clinical School of Veterinary Medicine and Animal Science São Paulo State University júlio de Mesquita Filho-UNESPDepartment of Surgical Specialties and Anesthesiology Urology School of Medicine São Paulo State University ¨júlio de Mesquita Filho-UNESPEnvol BiomedicalDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University júlio de Mesquita Filho-UNESP, Rua Prof. Dr. Walter Maurício Correa, s/n, Rubião JuniorDepartment of Veterinary Clinical School of Veterinary Medicine and Animal Science São Paulo State University júlio de Mesquita Filho-UNESPDepartment of Surgical Specialties and Anesthesiology Urology School of Medicine São Paulo State University ¨júlio de Mesquita Filho-UNESPUniversidade Estadual Paulista (Unesp)Envol BiomedicalCarvalho, Marcos G. [UNESP]Silva, Kelry M. [UNESP]Aristizabal, Viviana H. V. [UNESP]Ortiz, Pablo E. O. [UNESP]Paranzini, Cristiane S. [UNESP]Melchert, Alessandra [UNESP]Amaro, João L. [UNESP]Souza, Fabiana F. [UNESP]2021-06-25T10:57:04Z2021-06-25T10:57:04Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1021/acs.jproteome.0c01044Journal of Proteome Research.1535-39071535-3893http://hdl.handle.net/11449/20754610.1021/acs.jproteome.0c010442-s2.0-85103438247Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteome Researchinfo:eu-repo/semantics/openAccess2024-09-09T14:06:16Zoai:repositorio.unesp.br:11449/207546Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
title |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
spellingShingle |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats Carvalho, Marcos G. [UNESP] diabetic obese rat sperm metabolism sperm protein spermatogenesis |
title_short |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
title_full |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
title_fullStr |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
title_full_unstemmed |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
title_sort |
Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats |
author |
Carvalho, Marcos G. [UNESP] |
author_facet |
Carvalho, Marcos G. [UNESP] Silva, Kelry M. [UNESP] Aristizabal, Viviana H. V. [UNESP] Ortiz, Pablo E. O. [UNESP] Paranzini, Cristiane S. [UNESP] Melchert, Alessandra [UNESP] Amaro, João L. [UNESP] Souza, Fabiana F. [UNESP] |
author_role |
author |
author2 |
Silva, Kelry M. [UNESP] Aristizabal, Viviana H. V. [UNESP] Ortiz, Pablo E. O. [UNESP] Paranzini, Cristiane S. [UNESP] Melchert, Alessandra [UNESP] Amaro, João L. [UNESP] Souza, Fabiana F. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Envol Biomedical |
dc.contributor.author.fl_str_mv |
Carvalho, Marcos G. [UNESP] Silva, Kelry M. [UNESP] Aristizabal, Viviana H. V. [UNESP] Ortiz, Pablo E. O. [UNESP] Paranzini, Cristiane S. [UNESP] Melchert, Alessandra [UNESP] Amaro, João L. [UNESP] Souza, Fabiana F. [UNESP] |
dc.subject.por.fl_str_mv |
diabetic obese rat sperm metabolism sperm protein spermatogenesis |
topic |
diabetic obese rat sperm metabolism sperm protein spermatogenesis |
description |
Infertility caused by male factors is potentially associated with metabolic disorders such as obesity and/or diabetes. This experimental study was conducted in a male rodent model to assess the effects of different diseases on semen quality and sperm proteomics. Ten Wistar rats were used for each treatment. Rats were fed commercial food provided controllably to the control group and the diabetic group, and a hypercaloric diet supplemented with 5% sucrose in water was provided ad libitum to the obese group for 38 weeks. Diabetes was induced with 35 mg/kg streptozotocin. After euthanasia, testicles, spermatozoa, fat, and blood (serum) samples were collected. Spermatozoa were evaluated for quality and subjected to proteomics analyses. Histology and cytology of the testis, and serum leptin, adiponectin, interleukin 8 (IL-8), blood glucose, and testosterone levels, were also assessed. Body weight, retroperitoneal and testicular fat, and the Lee index were also measured. Obesity and diabetes were induced. The diabetic group showed noticeable changes in spermatogenesis and sperm quality. The mass spectrometry proteomics data have been deposited in Mendeley Data (doi: 10.17632/rfp7kfjcsd.5). Fifteen proteins varied in abundance between groups, especially proteins related to energy production and structural function of the spermatozoa, suggesting disturbances in energy production with a subsequent alteration in sperm motility in both groups, but with a compensatory response in the obese group. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:57:04Z 2021-06-25T10:57:04Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acs.jproteome.0c01044 Journal of Proteome Research. 1535-3907 1535-3893 http://hdl.handle.net/11449/207546 10.1021/acs.jproteome.0c01044 2-s2.0-85103438247 |
url |
http://dx.doi.org/10.1021/acs.jproteome.0c01044 http://hdl.handle.net/11449/207546 |
identifier_str_mv |
Journal of Proteome Research. 1535-3907 1535-3893 10.1021/acs.jproteome.0c01044 2-s2.0-85103438247 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteome Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021349279662080 |