SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus

Detalhes bibliográficos
Autor(a) principal: Mastrochirico-Filho, Vito Antonio [UNESP]
Data de Publicação: 2016
Outros Autores: Hata, Milene Elissa [UNESP], Sato, Lucas Seiti [UNESP], Jorge, Paulo Henrique [UNESP], Foresti, Fausto [UNESP], Rodriguez, Manuel Vera, Martínez, Paulino, Porto-Foresti, Fábio [UNESP], Hashimoto, Diogo Teruo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12686-016-0521-3
http://hdl.handle.net/11449/173136
Resumo: Pacu (Piaractus mesopotamicus) is a Neotropical freshwater fish threatened by overfishing, and one of the species of highest commercial value for aquaculture. Genetic variability analysis through molecular markers is an essential technique to genetic management of the wild and cultivated stocks. The main objective of this study was to identify and validate gene-associated SNPs of the liver transcriptome of pacu. Through genetic analysis in one natural population (Paraná River), 32 polymorphic SNPs were successfully genotyped and validated, some of them related to immune system genes. The observed and expected heterozygosity ranged from 0.059 to 0.706 and 0.058 to 0.507, respectively. All loci were in Hardy–Weinberg equilibrium (P > 0.05). Our results showed useful genomic resources for pacu, with applicability in conservation purposes and aquaculture industry by using SNPs markers.
id UNSP_fed462b86cb5656aa84d4988fcc27dc2
oai_identifier_str oai:repositorio.unesp.br:11449/173136
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling SNP discovery from liver transcriptome in the fish Piaractus mesopotamicusAquacultureGenetic variabilityNGSPacuPacu (Piaractus mesopotamicus) is a Neotropical freshwater fish threatened by overfishing, and one of the species of highest commercial value for aquaculture. Genetic variability analysis through molecular markers is an essential technique to genetic management of the wild and cultivated stocks. The main objective of this study was to identify and validate gene-associated SNPs of the liver transcriptome of pacu. Through genetic analysis in one natural population (Paraná River), 32 polymorphic SNPs were successfully genotyped and validated, some of them related to immune system genes. The observed and expected heterozygosity ranged from 0.059 to 0.706 and 0.058 to 0.507, respectively. All loci were in Hardy–Weinberg equilibrium (P > 0.05). Our results showed useful genomic resources for pacu, with applicability in conservation purposes and aquaculture industry by using SNPs markers.Centro de Aquicultura da Unesp Universidade Estadual PaulistaDepartamento de Morfologia IBB Universidade Estadual PaulistaDepartamento de Genética Facultad de Veterinaria Universidad de Santiago de CompostelaDepartamento de Ciências Biológicas FC Universidade Estadual PaulistaCentro de Aquicultura da Unesp Universidade Estadual PaulistaDepartamento de Morfologia IBB Universidade Estadual PaulistaDepartamento de Ciências Biológicas FC Universidade Estadual PaulistaUniversidade Estadual Paulista (Unesp)Universidad de Santiago de CompostelaMastrochirico-Filho, Vito Antonio [UNESP]Hata, Milene Elissa [UNESP]Sato, Lucas Seiti [UNESP]Jorge, Paulo Henrique [UNESP]Foresti, Fausto [UNESP]Rodriguez, Manuel VeraMartínez, PaulinoPorto-Foresti, Fábio [UNESP]Hashimoto, Diogo Teruo [UNESP]2018-12-11T17:03:48Z2018-12-11T17:03:48Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article109-114application/pdfhttp://dx.doi.org/10.1007/s12686-016-0521-3Conservation Genetics Resources, v. 8, n. 2, p. 109-114, 2016.1877-72601877-7252http://hdl.handle.net/11449/17313610.1007/s12686-016-0521-32-s2.0-849763181322-s2.0-84976318132.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengConservation Genetics Resources0,4300,430info:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/173136Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:57:35.981298Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
title SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
spellingShingle SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
Mastrochirico-Filho, Vito Antonio [UNESP]
Aquaculture
Genetic variability
NGS
Pacu
title_short SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
title_full SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
title_fullStr SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
title_full_unstemmed SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
title_sort SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus
author Mastrochirico-Filho, Vito Antonio [UNESP]
author_facet Mastrochirico-Filho, Vito Antonio [UNESP]
Hata, Milene Elissa [UNESP]
Sato, Lucas Seiti [UNESP]
Jorge, Paulo Henrique [UNESP]
Foresti, Fausto [UNESP]
Rodriguez, Manuel Vera
Martínez, Paulino
Porto-Foresti, Fábio [UNESP]
Hashimoto, Diogo Teruo [UNESP]
author_role author
author2 Hata, Milene Elissa [UNESP]
Sato, Lucas Seiti [UNESP]
Jorge, Paulo Henrique [UNESP]
Foresti, Fausto [UNESP]
Rodriguez, Manuel Vera
Martínez, Paulino
Porto-Foresti, Fábio [UNESP]
Hashimoto, Diogo Teruo [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidad de Santiago de Compostela
dc.contributor.author.fl_str_mv Mastrochirico-Filho, Vito Antonio [UNESP]
Hata, Milene Elissa [UNESP]
Sato, Lucas Seiti [UNESP]
Jorge, Paulo Henrique [UNESP]
Foresti, Fausto [UNESP]
Rodriguez, Manuel Vera
Martínez, Paulino
Porto-Foresti, Fábio [UNESP]
Hashimoto, Diogo Teruo [UNESP]
dc.subject.por.fl_str_mv Aquaculture
Genetic variability
NGS
Pacu
topic Aquaculture
Genetic variability
NGS
Pacu
description Pacu (Piaractus mesopotamicus) is a Neotropical freshwater fish threatened by overfishing, and one of the species of highest commercial value for aquaculture. Genetic variability analysis through molecular markers is an essential technique to genetic management of the wild and cultivated stocks. The main objective of this study was to identify and validate gene-associated SNPs of the liver transcriptome of pacu. Through genetic analysis in one natural population (Paraná River), 32 polymorphic SNPs were successfully genotyped and validated, some of them related to immune system genes. The observed and expected heterozygosity ranged from 0.059 to 0.706 and 0.058 to 0.507, respectively. All loci were in Hardy–Weinberg equilibrium (P > 0.05). Our results showed useful genomic resources for pacu, with applicability in conservation purposes and aquaculture industry by using SNPs markers.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
2018-12-11T17:03:48Z
2018-12-11T17:03:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12686-016-0521-3
Conservation Genetics Resources, v. 8, n. 2, p. 109-114, 2016.
1877-7260
1877-7252
http://hdl.handle.net/11449/173136
10.1007/s12686-016-0521-3
2-s2.0-84976318132
2-s2.0-84976318132.pdf
url http://dx.doi.org/10.1007/s12686-016-0521-3
http://hdl.handle.net/11449/173136
identifier_str_mv Conservation Genetics Resources, v. 8, n. 2, p. 109-114, 2016.
1877-7260
1877-7252
10.1007/s12686-016-0521-3
2-s2.0-84976318132
2-s2.0-84976318132.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Conservation Genetics Resources
0,430
0,430
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 109-114
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129268766474240

Registros relacionados