Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.yrtph.2022.105118 http://hdl.handle.net/11449/234025 |
Resumo: | Piper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period. |
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Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studiesGenotoxicityHemato-biochemical alterationsHepatotoxicityMicePiper amalagoPiperaceaeRatsSafetySubacute toxicityPiper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Structural and Functional Biology Institute of Biosciences of Botucatu UNESP – Univ. Estadual Paulista - Botucatu, São Paulo StateSchool of Health Sciences University Center of Grande Dourados UNIGRANMato Grosso do Sul State University UEMSFederal University of Mato Grosso do Sul UFMSSchool of Health Sciences Federal University of Grande Dourados UFGDDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu UNESP – Univ. Estadual Paulista - Botucatu, São Paulo StateCNPq: #304266/2017–5CNPq: #311166/2016–4CNPq: #427859/2018–2Universidade Estadual Paulista (UNESP)UNIGRANUniversidade Estadual de Mato Grosso do Sul (UEMS)Universidade Federal de Mato Grosso do Sul (UFMS)UFGDStein, Julia [UNESP]Jorge, Bárbara Campos [UNESP]Casali Reis, Ana Carolina [UNESP]Santos Radai, Joyce Alencarda Silva Moreira, Suyane [UNESP]Fraga, Thiago Leiteda Silva Mota, JonasOliveira, Rodrigo JulianoKassuya, Cândida Aparecida LeiteArena, Arielle Cristina [UNESP]2022-05-01T12:40:46Z2022-05-01T12:40:46Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.yrtph.2022.105118Regulatory Toxicology and Pharmacology, v. 129.1096-02950273-2300http://hdl.handle.net/11449/23402510.1016/j.yrtph.2022.1051182-s2.0-85122987191Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRegulatory Toxicology and Pharmacologyinfo:eu-repo/semantics/openAccess2022-05-01T12:40:46Zoai:repositorio.unesp.br:11449/234025Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:53:23.882328Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
title |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
spellingShingle |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies Stein, Julia [UNESP] Genotoxicity Hemato-biochemical alterations Hepatotoxicity Mice Piper amalago Piperaceae Rats Safety Subacute toxicity |
title_short |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
title_full |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
title_fullStr |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
title_full_unstemmed |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
title_sort |
Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies |
author |
Stein, Julia [UNESP] |
author_facet |
Stein, Julia [UNESP] Jorge, Bárbara Campos [UNESP] Casali Reis, Ana Carolina [UNESP] Santos Radai, Joyce Alencar da Silva Moreira, Suyane [UNESP] Fraga, Thiago Leite da Silva Mota, Jonas Oliveira, Rodrigo Juliano Kassuya, Cândida Aparecida Leite Arena, Arielle Cristina [UNESP] |
author_role |
author |
author2 |
Jorge, Bárbara Campos [UNESP] Casali Reis, Ana Carolina [UNESP] Santos Radai, Joyce Alencar da Silva Moreira, Suyane [UNESP] Fraga, Thiago Leite da Silva Mota, Jonas Oliveira, Rodrigo Juliano Kassuya, Cândida Aparecida Leite Arena, Arielle Cristina [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) UNIGRAN Universidade Estadual de Mato Grosso do Sul (UEMS) Universidade Federal de Mato Grosso do Sul (UFMS) UFGD |
dc.contributor.author.fl_str_mv |
Stein, Julia [UNESP] Jorge, Bárbara Campos [UNESP] Casali Reis, Ana Carolina [UNESP] Santos Radai, Joyce Alencar da Silva Moreira, Suyane [UNESP] Fraga, Thiago Leite da Silva Mota, Jonas Oliveira, Rodrigo Juliano Kassuya, Cândida Aparecida Leite Arena, Arielle Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Genotoxicity Hemato-biochemical alterations Hepatotoxicity Mice Piper amalago Piperaceae Rats Safety Subacute toxicity |
topic |
Genotoxicity Hemato-biochemical alterations Hepatotoxicity Mice Piper amalago Piperaceae Rats Safety Subacute toxicity |
description |
Piper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T12:40:46Z 2022-05-01T12:40:46Z 2022-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.yrtph.2022.105118 Regulatory Toxicology and Pharmacology, v. 129. 1096-0295 0273-2300 http://hdl.handle.net/11449/234025 10.1016/j.yrtph.2022.105118 2-s2.0-85122987191 |
url |
http://dx.doi.org/10.1016/j.yrtph.2022.105118 http://hdl.handle.net/11449/234025 |
identifier_str_mv |
Regulatory Toxicology and Pharmacology, v. 129. 1096-0295 0273-2300 10.1016/j.yrtph.2022.105118 2-s2.0-85122987191 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Regulatory Toxicology and Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128288102547456 |