Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies

Detalhes bibliográficos
Autor(a) principal: Stein, Julia [UNESP]
Data de Publicação: 2022
Outros Autores: Jorge, Bárbara Campos [UNESP], Casali Reis, Ana Carolina [UNESP], Santos Radai, Joyce Alencar, da Silva Moreira, Suyane [UNESP], Fraga, Thiago Leite, da Silva Mota, Jonas, Oliveira, Rodrigo Juliano, Kassuya, Cândida Aparecida Leite, Arena, Arielle Cristina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.yrtph.2022.105118
http://hdl.handle.net/11449/234025
Resumo: Piper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period.
id UNSP_fedefcc694a4a23931bd21dfab628c7f
oai_identifier_str oai:repositorio.unesp.br:11449/234025
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studiesGenotoxicityHemato-biochemical alterationsHepatotoxicityMicePiper amalagoPiperaceaeRatsSafetySubacute toxicityPiper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Structural and Functional Biology Institute of Biosciences of Botucatu UNESP – Univ. Estadual Paulista - Botucatu, São Paulo StateSchool of Health Sciences University Center of Grande Dourados UNIGRANMato Grosso do Sul State University UEMSFederal University of Mato Grosso do Sul UFMSSchool of Health Sciences Federal University of Grande Dourados UFGDDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu UNESP – Univ. Estadual Paulista - Botucatu, São Paulo StateCNPq: #304266/2017–5CNPq: #311166/2016–4CNPq: #427859/2018–2Universidade Estadual Paulista (UNESP)UNIGRANUniversidade Estadual de Mato Grosso do Sul (UEMS)Universidade Federal de Mato Grosso do Sul (UFMS)UFGDStein, Julia [UNESP]Jorge, Bárbara Campos [UNESP]Casali Reis, Ana Carolina [UNESP]Santos Radai, Joyce Alencarda Silva Moreira, Suyane [UNESP]Fraga, Thiago Leiteda Silva Mota, JonasOliveira, Rodrigo JulianoKassuya, Cândida Aparecida LeiteArena, Arielle Cristina [UNESP]2022-05-01T12:40:46Z2022-05-01T12:40:46Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.yrtph.2022.105118Regulatory Toxicology and Pharmacology, v. 129.1096-02950273-2300http://hdl.handle.net/11449/23402510.1016/j.yrtph.2022.1051182-s2.0-85122987191Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRegulatory Toxicology and Pharmacologyinfo:eu-repo/semantics/openAccess2022-05-01T12:40:46Zoai:repositorio.unesp.br:11449/234025Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:53:23.882328Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
title Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
spellingShingle Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
Stein, Julia [UNESP]
Genotoxicity
Hemato-biochemical alterations
Hepatotoxicity
Mice
Piper amalago
Piperaceae
Rats
Safety
Subacute toxicity
title_short Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
title_full Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
title_fullStr Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
title_full_unstemmed Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
title_sort Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies
author Stein, Julia [UNESP]
author_facet Stein, Julia [UNESP]
Jorge, Bárbara Campos [UNESP]
Casali Reis, Ana Carolina [UNESP]
Santos Radai, Joyce Alencar
da Silva Moreira, Suyane [UNESP]
Fraga, Thiago Leite
da Silva Mota, Jonas
Oliveira, Rodrigo Juliano
Kassuya, Cândida Aparecida Leite
Arena, Arielle Cristina [UNESP]
author_role author
author2 Jorge, Bárbara Campos [UNESP]
Casali Reis, Ana Carolina [UNESP]
Santos Radai, Joyce Alencar
da Silva Moreira, Suyane [UNESP]
Fraga, Thiago Leite
da Silva Mota, Jonas
Oliveira, Rodrigo Juliano
Kassuya, Cândida Aparecida Leite
Arena, Arielle Cristina [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
UNIGRAN
Universidade Estadual de Mato Grosso do Sul (UEMS)
Universidade Federal de Mato Grosso do Sul (UFMS)
UFGD
dc.contributor.author.fl_str_mv Stein, Julia [UNESP]
Jorge, Bárbara Campos [UNESP]
Casali Reis, Ana Carolina [UNESP]
Santos Radai, Joyce Alencar
da Silva Moreira, Suyane [UNESP]
Fraga, Thiago Leite
da Silva Mota, Jonas
Oliveira, Rodrigo Juliano
Kassuya, Cândida Aparecida Leite
Arena, Arielle Cristina [UNESP]
dc.subject.por.fl_str_mv Genotoxicity
Hemato-biochemical alterations
Hepatotoxicity
Mice
Piper amalago
Piperaceae
Rats
Safety
Subacute toxicity
topic Genotoxicity
Hemato-biochemical alterations
Hepatotoxicity
Mice
Piper amalago
Piperaceae
Rats
Safety
Subacute toxicity
description Piper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01T12:40:46Z
2022-05-01T12:40:46Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.yrtph.2022.105118
Regulatory Toxicology and Pharmacology, v. 129.
1096-0295
0273-2300
http://hdl.handle.net/11449/234025
10.1016/j.yrtph.2022.105118
2-s2.0-85122987191
url http://dx.doi.org/10.1016/j.yrtph.2022.105118
http://hdl.handle.net/11449/234025
identifier_str_mv Regulatory Toxicology and Pharmacology, v. 129.
1096-0295
0273-2300
10.1016/j.yrtph.2022.105118
2-s2.0-85122987191
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Regulatory Toxicology and Pharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128288102547456