Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fgene.2021.742870 http://hdl.handle.net/11449/233942 |
Resumo: | The red brocket deer Mazama americana Erxleben, 1777 is considered a polyphyletic complex of cryptic species with wide chromosomal divergence. Evidence indicates that the observed chromosomal divergences result in reproductive isolation. The description of a neotype for M. americana allowed its genetic characterization and represented a comparative basis to resolve the taxonomic uncertainties of the group. Thus, we designated a neotype for the synonym Mazama rufa Illiger, 1815 and tested its recognition as a distinct species from the M. americana complex with the analysis of morphological, cytogenetic and molecular data. We also evaluated its distribution by sampling fecal DNA in the wild. Morphological data from craniometry and body biometry indicated an overlap of quantitative measurements between M. rufa and the entire M. americana complex. The phylogenetic hypothesis obtained through mtDNA confirmed the reciprocal monophyly relationship between M. americana and M. rufa, and both were identified as distinct molecular operational taxonomic units by the General Mixed Yule Coalescent species delimitation analysis. Finally, classic cytogenetic data and fluorescence in situ hybridization with whole chromosome painting probes showed M. rufa with a karyotype of 2n = 52, FN = 56. Comparative analysis indicate that at least fifteen rearrangements separate M. rufa and M. americana (sensu stricto) karyotypes, which confirmed their substantial chromosomal divergence. This divergence should represent an important reproductive barrier and allow its characterization as a distinct and valid species. Genetic analysis of fecal samples demonstrated a wide distribution of M. rufa in the South American continent through the Atlantic Forest, Cerrado and south region of Amazon. Thus, we conclude for the revalidation of M. rufa as a distinct species under the concept of biological isolation, with its karyotype as the main diagnostic character. The present work serves as a basis for the taxonomic review of the M. americana complex, which should be mainly based on cytogenetic characterization and directed towards a better sampling of the Amazon region, the evaluation of available names in the species synonymy and a multi-locus phylogenetic analysis. |
id |
UNSP_ff181d8c9b1cf59de3f6a837da655ce0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/233942 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777)bayesian phylogenetic inferencecytotaxonomy, molecular cytogeneticsGMYCnon-invasive samplingOdocoileiniscat detection dogThe red brocket deer Mazama americana Erxleben, 1777 is considered a polyphyletic complex of cryptic species with wide chromosomal divergence. Evidence indicates that the observed chromosomal divergences result in reproductive isolation. The description of a neotype for M. americana allowed its genetic characterization and represented a comparative basis to resolve the taxonomic uncertainties of the group. Thus, we designated a neotype for the synonym Mazama rufa Illiger, 1815 and tested its recognition as a distinct species from the M. americana complex with the analysis of morphological, cytogenetic and molecular data. We also evaluated its distribution by sampling fecal DNA in the wild. Morphological data from craniometry and body biometry indicated an overlap of quantitative measurements between M. rufa and the entire M. americana complex. The phylogenetic hypothesis obtained through mtDNA confirmed the reciprocal monophyly relationship between M. americana and M. rufa, and both were identified as distinct molecular operational taxonomic units by the General Mixed Yule Coalescent species delimitation analysis. Finally, classic cytogenetic data and fluorescence in situ hybridization with whole chromosome painting probes showed M. rufa with a karyotype of 2n = 52, FN = 56. Comparative analysis indicate that at least fifteen rearrangements separate M. rufa and M. americana (sensu stricto) karyotypes, which confirmed their substantial chromosomal divergence. This divergence should represent an important reproductive barrier and allow its characterization as a distinct and valid species. Genetic analysis of fecal samples demonstrated a wide distribution of M. rufa in the South American continent through the Atlantic Forest, Cerrado and south region of Amazon. Thus, we conclude for the revalidation of M. rufa as a distinct species under the concept of biological isolation, with its karyotype as the main diagnostic character. The present work serves as a basis for the taxonomic review of the M. americana complex, which should be mainly based on cytogenetic characterization and directed towards a better sampling of the Amazon region, the evaluation of available names in the species synonymy and a multi-locus phylogenetic analysis.Deer Research and Conservation Center (NUPECCE) São Paulo State University (UNESP)Faculty of Veterinary Medicine National University of San Marcos (UNMSM)Medical School University Center of Adamantina (UniFAI)Veterinary Research InstituteDeer Research and Conservation Center (NUPECCE) São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)National University of San Marcos (UNMSM)University Center of Adamantina (UniFAI)Veterinary Research InstitutePeres, Pedro H. F. [UNESP]Luduvério, Douglas J. [UNESP]Bernegossi, Agda Maria [UNESP]Galindo, David J.Nascimento, Guilherme B.Oliveira, Márcio L. [UNESP]Sandoval, Eluzai Dinai Pinto [UNESP]Vozdova, MiluseKubickova, SvatavaCernohorska, HalinaDuarte, José Maurício Barbanti [UNESP]2022-05-01T11:54:07Z2022-05-01T11:54:07Z2021-12-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fgene.2021.742870Frontiers in Genetics, v. 12.1664-8021http://hdl.handle.net/11449/23394210.3389/fgene.2021.7428702-s2.0-85121851408Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Geneticsinfo:eu-repo/semantics/openAccess2022-05-01T11:54:07Zoai:repositorio.unesp.br:11449/233942Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T11:54:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
title |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
spellingShingle |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) Peres, Pedro H. F. [UNESP] bayesian phylogenetic inference cytotaxonomy, molecular cytogenetics GMYC non-invasive sampling Odocoileini scat detection dog |
title_short |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
title_full |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
title_fullStr |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
title_full_unstemmed |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
title_sort |
Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777) |
author |
Peres, Pedro H. F. [UNESP] |
author_facet |
Peres, Pedro H. F. [UNESP] Luduvério, Douglas J. [UNESP] Bernegossi, Agda Maria [UNESP] Galindo, David J. Nascimento, Guilherme B. Oliveira, Márcio L. [UNESP] Sandoval, Eluzai Dinai Pinto [UNESP] Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Duarte, José Maurício Barbanti [UNESP] |
author_role |
author |
author2 |
Luduvério, Douglas J. [UNESP] Bernegossi, Agda Maria [UNESP] Galindo, David J. Nascimento, Guilherme B. Oliveira, Márcio L. [UNESP] Sandoval, Eluzai Dinai Pinto [UNESP] Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Duarte, José Maurício Barbanti [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) National University of San Marcos (UNMSM) University Center of Adamantina (UniFAI) Veterinary Research Institute |
dc.contributor.author.fl_str_mv |
Peres, Pedro H. F. [UNESP] Luduvério, Douglas J. [UNESP] Bernegossi, Agda Maria [UNESP] Galindo, David J. Nascimento, Guilherme B. Oliveira, Márcio L. [UNESP] Sandoval, Eluzai Dinai Pinto [UNESP] Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Duarte, José Maurício Barbanti [UNESP] |
dc.subject.por.fl_str_mv |
bayesian phylogenetic inference cytotaxonomy, molecular cytogenetics GMYC non-invasive sampling Odocoileini scat detection dog |
topic |
bayesian phylogenetic inference cytotaxonomy, molecular cytogenetics GMYC non-invasive sampling Odocoileini scat detection dog |
description |
The red brocket deer Mazama americana Erxleben, 1777 is considered a polyphyletic complex of cryptic species with wide chromosomal divergence. Evidence indicates that the observed chromosomal divergences result in reproductive isolation. The description of a neotype for M. americana allowed its genetic characterization and represented a comparative basis to resolve the taxonomic uncertainties of the group. Thus, we designated a neotype for the synonym Mazama rufa Illiger, 1815 and tested its recognition as a distinct species from the M. americana complex with the analysis of morphological, cytogenetic and molecular data. We also evaluated its distribution by sampling fecal DNA in the wild. Morphological data from craniometry and body biometry indicated an overlap of quantitative measurements between M. rufa and the entire M. americana complex. The phylogenetic hypothesis obtained through mtDNA confirmed the reciprocal monophyly relationship between M. americana and M. rufa, and both were identified as distinct molecular operational taxonomic units by the General Mixed Yule Coalescent species delimitation analysis. Finally, classic cytogenetic data and fluorescence in situ hybridization with whole chromosome painting probes showed M. rufa with a karyotype of 2n = 52, FN = 56. Comparative analysis indicate that at least fifteen rearrangements separate M. rufa and M. americana (sensu stricto) karyotypes, which confirmed their substantial chromosomal divergence. This divergence should represent an important reproductive barrier and allow its characterization as a distinct and valid species. Genetic analysis of fecal samples demonstrated a wide distribution of M. rufa in the South American continent through the Atlantic Forest, Cerrado and south region of Amazon. Thus, we conclude for the revalidation of M. rufa as a distinct species under the concept of biological isolation, with its karyotype as the main diagnostic character. The present work serves as a basis for the taxonomic review of the M. americana complex, which should be mainly based on cytogenetic characterization and directed towards a better sampling of the Amazon region, the evaluation of available names in the species synonymy and a multi-locus phylogenetic analysis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-14 2022-05-01T11:54:07Z 2022-05-01T11:54:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fgene.2021.742870 Frontiers in Genetics, v. 12. 1664-8021 http://hdl.handle.net/11449/233942 10.3389/fgene.2021.742870 2-s2.0-85121851408 |
url |
http://dx.doi.org/10.3389/fgene.2021.742870 http://hdl.handle.net/11449/233942 |
identifier_str_mv |
Frontiers in Genetics, v. 12. 1664-8021 10.3389/fgene.2021.742870 2-s2.0-85121851408 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965662512152576 |