Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ani13010060 http://hdl.handle.net/11449/246614 |
Resumo: | This study aimed to investigate the pro-apoptotic effects of NSAID (Previcox®) in vitro and in vivo. Two CMT cell lines, one from the primary tumor and one from bone metastasis, were treated with firocoxib and MTT assay was performed to determine the half-maximal inhibitory concentration (IC50) value. The firocoxib IC50 for the cell lines UNESP-CM5 and UNESP-MM1 were 25.21 µM and 27.41 µM, respectively. The cell lines were then treated with the respective firocoxib IC50 concentrations and annexin V/propidium iodide (PI) assay was performed, to detect the induction of apoptosis in both cells (Annexin+/PI+). We conducted an in vivo study involving female dogs affected by CMT and divided them into control and treatment groups. For both groups, a biopsy was performed on day 0 (D0) and a mastectomy was performed on day 14 (D14). In the treatment group, after biopsy on D0, the patients received Previcox® 5 mg/kg PO once a day until mastectomy was performed on D14. COX-2/caspase-3 double immunostaining was performed on samples from D0 and D14, revealing no difference in the control group. In contrast, in the treatment group Previcox® increased the number of COX-2 positive apoptotic cells. Therefore, firocoxib can induce apoptosis in CMT cells in vitro and in vivo, and Previcox® can be a potential neoadjuvant treatment for patients with mammary cancer. |
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Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumorscaspase-3chemotherapycyclooxygenase-2nonsteroidal anti-inflammatory drugThis study aimed to investigate the pro-apoptotic effects of NSAID (Previcox®) in vitro and in vivo. Two CMT cell lines, one from the primary tumor and one from bone metastasis, were treated with firocoxib and MTT assay was performed to determine the half-maximal inhibitory concentration (IC50) value. The firocoxib IC50 for the cell lines UNESP-CM5 and UNESP-MM1 were 25.21 µM and 27.41 µM, respectively. The cell lines were then treated with the respective firocoxib IC50 concentrations and annexin V/propidium iodide (PI) assay was performed, to detect the induction of apoptosis in both cells (Annexin+/PI+). We conducted an in vivo study involving female dogs affected by CMT and divided them into control and treatment groups. For both groups, a biopsy was performed on day 0 (D0) and a mastectomy was performed on day 14 (D14). In the treatment group, after biopsy on D0, the patients received Previcox® 5 mg/kg PO once a day until mastectomy was performed on D14. COX-2/caspase-3 double immunostaining was performed on samples from D0 and D14, revealing no difference in the control group. In contrast, in the treatment group Previcox® increased the number of COX-2 positive apoptotic cells. Therefore, firocoxib can induce apoptosis in CMT cells in vitro and in vivo, and Previcox® can be a potential neoadjuvant treatment for patients with mammary cancer.Department of Veterinary Surgery and Animal Reproduction São Paulo State University-UNESPDepartment of Veterinary Medicine and Animal Science University of Milan, Via dell’ Università 6Veterinary School Federal University of Fronteira Sul-UFFSCEVEPAT Veterinary Diagnostic LaboratoryDepartment of Veterinary Clinic Sao Paulo State University-UNESPDepartment of Pathology University of São Paulo-USPInstitute of Health Sciences Paulista University-UNIPDepartment of Veterinary Surgery and Animal Reproduction São Paulo State University-UNESPDepartment of Veterinary Clinic Sao Paulo State University-UNESPUniversidade Estadual Paulista (UNESP)University of MilanFederal University of Fronteira Sul-UFFSCEVEPAT Veterinary Diagnostic LaboratoryUniversidade de São Paulo (USP)Paulista University-UNIPBrandi, Andressa [UNESP]de Faria Lainetti, Patricia [UNESP]Elias, FabianaRodrigues, Marcela Marcondes PintoFagundes Moraes, LiviaLaufer-Amorim, Renée [UNESP]de Camargo, Laíza Sartori [UNESP]Salles Gomes, Cristina de Oliveira MassocoFonseca-Alves, Carlos Eduardo [UNESP]2023-07-29T12:45:48Z2023-07-29T12:45:48Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani13010060Animals, v. 13, n. 1, 2023.2076-2615http://hdl.handle.net/11449/24661410.3390/ani130100602-s2.0-85145845481Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2023-07-29T12:45:48Zoai:repositorio.unesp.br:11449/246614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:17.486509Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
title |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
spellingShingle |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors Brandi, Andressa [UNESP] caspase-3 chemotherapy cyclooxygenase-2 nonsteroidal anti-inflammatory drug |
title_short |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
title_full |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
title_fullStr |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
title_full_unstemmed |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
title_sort |
Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors |
author |
Brandi, Andressa [UNESP] |
author_facet |
Brandi, Andressa [UNESP] de Faria Lainetti, Patricia [UNESP] Elias, Fabiana Rodrigues, Marcela Marcondes Pinto Fagundes Moraes, Livia Laufer-Amorim, Renée [UNESP] de Camargo, Laíza Sartori [UNESP] Salles Gomes, Cristina de Oliveira Massoco Fonseca-Alves, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
de Faria Lainetti, Patricia [UNESP] Elias, Fabiana Rodrigues, Marcela Marcondes Pinto Fagundes Moraes, Livia Laufer-Amorim, Renée [UNESP] de Camargo, Laíza Sartori [UNESP] Salles Gomes, Cristina de Oliveira Massoco Fonseca-Alves, Carlos Eduardo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Milan Federal University of Fronteira Sul-UFFS CEVEPAT Veterinary Diagnostic Laboratory Universidade de São Paulo (USP) Paulista University-UNIP |
dc.contributor.author.fl_str_mv |
Brandi, Andressa [UNESP] de Faria Lainetti, Patricia [UNESP] Elias, Fabiana Rodrigues, Marcela Marcondes Pinto Fagundes Moraes, Livia Laufer-Amorim, Renée [UNESP] de Camargo, Laíza Sartori [UNESP] Salles Gomes, Cristina de Oliveira Massoco Fonseca-Alves, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
caspase-3 chemotherapy cyclooxygenase-2 nonsteroidal anti-inflammatory drug |
topic |
caspase-3 chemotherapy cyclooxygenase-2 nonsteroidal anti-inflammatory drug |
description |
This study aimed to investigate the pro-apoptotic effects of NSAID (Previcox®) in vitro and in vivo. Two CMT cell lines, one from the primary tumor and one from bone metastasis, were treated with firocoxib and MTT assay was performed to determine the half-maximal inhibitory concentration (IC50) value. The firocoxib IC50 for the cell lines UNESP-CM5 and UNESP-MM1 were 25.21 µM and 27.41 µM, respectively. The cell lines were then treated with the respective firocoxib IC50 concentrations and annexin V/propidium iodide (PI) assay was performed, to detect the induction of apoptosis in both cells (Annexin+/PI+). We conducted an in vivo study involving female dogs affected by CMT and divided them into control and treatment groups. For both groups, a biopsy was performed on day 0 (D0) and a mastectomy was performed on day 14 (D14). In the treatment group, after biopsy on D0, the patients received Previcox® 5 mg/kg PO once a day until mastectomy was performed on D14. COX-2/caspase-3 double immunostaining was performed on samples from D0 and D14, revealing no difference in the control group. In contrast, in the treatment group Previcox® increased the number of COX-2 positive apoptotic cells. Therefore, firocoxib can induce apoptosis in CMT cells in vitro and in vivo, and Previcox® can be a potential neoadjuvant treatment for patients with mammary cancer. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:45:48Z 2023-07-29T12:45:48Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ani13010060 Animals, v. 13, n. 1, 2023. 2076-2615 http://hdl.handle.net/11449/246614 10.3390/ani13010060 2-s2.0-85145845481 |
url |
http://dx.doi.org/10.3390/ani13010060 http://hdl.handle.net/11449/246614 |
identifier_str_mv |
Animals, v. 13, n. 1, 2023. 2076-2615 10.3390/ani13010060 2-s2.0-85145845481 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128745940189184 |