Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Amanda [UNESP]
Data de Publicação: 2023
Outros Autores: Anholeto, Luís Adriano, Cola, Diego Faria [UNESP], Fantatto, Rafaela Regina [UNESP], Gainza, Yousmel Alemán [UNESP], dos Santos, Isabella Barbosa [UNESP], Viçozzi, Gabriel Pedroso, Ávila, Daiana Silva, Fraceto, Leonardo Fernandes [UNESP], Chagas, Ana Carolina de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2023.109918
http://hdl.handle.net/11449/249845
Resumo: Nanoformulations containing zein nanoparticles (ZN) can promote the stability and protection of molecules with acaricidal activity. The present study sought to develop nanoformulations with ZN associated with cypermethrin (CYPE) + chlorpyrifos (CHLO) + a plant compound (citral, menthol or limonene), characterize them, and verify their efficacy against Rhipicephalus microplus ticks. Additionally, we aimed to assess its safety in nontarget nematodes found in soil at a site subjected to contamination by acaricides. The nanoformulations were characterized by dynamic light scattering and nanoparticle tracking analysis. Nanoformulations 1 (ZN+CYPE+CHLO+citral), 2 (ZN+CYPE+CHLO+menthol), and 3 (ZN+CYPE+CHLO+limonene) were measured for diameter, polydispersion, zeta potential, concentration, and encapsulation efficiency. Nanoformulations 1, 2, and 3 were evaluated in a range from 0.004 to 0.466 mg/mL on R. microplus larvae and caused mortality > 80% at concentrations above 0.029 mg/mL. The commercial acaricide Colosso® (CYPE 15 g + CHLO 25 g + citronellal 1 g) was evaluated also from 0.004 to 0.512 mg/mL and resulted in 71.9% larval mortality at 0.064 mg/mL. Formulations 1, 2, and 3 at 0.466 mg/mL showed acaricidal efficacy of 50.2%, 40.5%, and 60.1% on engorged females, respectively, while Colosso® at 0.512 mg/mL obtained only 39.4%. The nanoformulations exhibited long residual period of activity and lower toxicity to nontarget nematodes. ZN was able to protect the active compounds against degradation during the storage period. Thus, ZN can be an alternative for the development of new acaricidal formulations using lower concentrations of active compounds.
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spelling Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplusAcaricide residuesNanocarrierNanoformulationsResistanceTick controlNanoformulations containing zein nanoparticles (ZN) can promote the stability and protection of molecules with acaricidal activity. The present study sought to develop nanoformulations with ZN associated with cypermethrin (CYPE) + chlorpyrifos (CHLO) + a plant compound (citral, menthol or limonene), characterize them, and verify their efficacy against Rhipicephalus microplus ticks. Additionally, we aimed to assess its safety in nontarget nematodes found in soil at a site subjected to contamination by acaricides. The nanoformulations were characterized by dynamic light scattering and nanoparticle tracking analysis. Nanoformulations 1 (ZN+CYPE+CHLO+citral), 2 (ZN+CYPE+CHLO+menthol), and 3 (ZN+CYPE+CHLO+limonene) were measured for diameter, polydispersion, zeta potential, concentration, and encapsulation efficiency. Nanoformulations 1, 2, and 3 were evaluated in a range from 0.004 to 0.466 mg/mL on R. microplus larvae and caused mortality > 80% at concentrations above 0.029 mg/mL. The commercial acaricide Colosso® (CYPE 15 g + CHLO 25 g + citronellal 1 g) was evaluated also from 0.004 to 0.512 mg/mL and resulted in 71.9% larval mortality at 0.064 mg/mL. Formulations 1, 2, and 3 at 0.466 mg/mL showed acaricidal efficacy of 50.2%, 40.5%, and 60.1% on engorged females, respectively, while Colosso® at 0.512 mg/mL obtained only 39.4%. The nanoformulations exhibited long residual period of activity and lower toxicity to nontarget nematodes. ZN was able to protect the active compounds against degradation during the storage period. Thus, ZN can be an alternative for the development of new acaricidal formulations using lower concentrations of active compounds.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Empresa Brasileira de Pesquisa AgropecuáriaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual PaulistaSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP), Rod. Prof. Paulo Donato Castellane, SPBrazilian Agricultural Research Corporation (EMBRAPA) Southeast Livestock Unit, Rod. Washington Luiz, Km 234 s/n, Fazenda Canchim, SPInstitute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de março, 511, Alto da Boa Vista, SPSchool of Pharmaceutical Sciences São Paulo State University (UNESP), Rod. Araraquara-Jaú, km 1, Campos Ville, SPLaboratory of Biochemistry and Toxicology in Caenorhabditis elegans Federal University of Pampa, BR 472, Km 592, RSSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP), Rod. Prof. Paulo Donato Castellane, SPInstitute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de março, 511, Alto da Boa Vista, SPSchool of Pharmaceutical Sciences São Paulo State University (UNESP), Rod. Araraquara-Jaú, km 1, Campos Ville, SPCNPq: 169777/2017-0Empresa Brasileira de Pesquisa Agropecuária: 20.18.03.17.00-02FAPESP: 2016/06784-1, 2017/21004-5, 2017/13249-8FAPESP: 2018/16070-1FAPESP: 2019/20185-1Universidade Estadual Paulista: 88882.434504/2019-01Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Federal University of PampaFigueiredo, Amanda [UNESP]Anholeto, Luís AdrianoCola, Diego Faria [UNESP]Fantatto, Rafaela Regina [UNESP]Gainza, Yousmel Alemán [UNESP]dos Santos, Isabella Barbosa [UNESP]Viçozzi, Gabriel PedrosoÁvila, Daiana SilvaFraceto, Leonardo Fernandes [UNESP]Chagas, Ana Carolina de Souza2023-07-29T16:10:48Z2023-07-29T16:10:48Z2023-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vetpar.2023.109918Veterinary Parasitology, v. 318.1873-25500304-4017http://hdl.handle.net/11449/24984510.1016/j.vetpar.2023.1099182-s2.0-85152097988Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitologyinfo:eu-repo/semantics/openAccess2023-07-29T16:10:48Zoai:repositorio.unesp.br:11449/249845Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:04:10.631461Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
title Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
spellingShingle Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
Figueiredo, Amanda [UNESP]
Acaricide residues
Nanocarrier
Nanoformulations
Resistance
Tick control
title_short Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
title_full Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
title_fullStr Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
title_full_unstemmed Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
title_sort Acaricides containing zein nanoparticles: A tool for a lower impact control of the cattle tick Rhipicephalus microplus
author Figueiredo, Amanda [UNESP]
author_facet Figueiredo, Amanda [UNESP]
Anholeto, Luís Adriano
Cola, Diego Faria [UNESP]
Fantatto, Rafaela Regina [UNESP]
Gainza, Yousmel Alemán [UNESP]
dos Santos, Isabella Barbosa [UNESP]
Viçozzi, Gabriel Pedroso
Ávila, Daiana Silva
Fraceto, Leonardo Fernandes [UNESP]
Chagas, Ana Carolina de Souza
author_role author
author2 Anholeto, Luís Adriano
Cola, Diego Faria [UNESP]
Fantatto, Rafaela Regina [UNESP]
Gainza, Yousmel Alemán [UNESP]
dos Santos, Isabella Barbosa [UNESP]
Viçozzi, Gabriel Pedroso
Ávila, Daiana Silva
Fraceto, Leonardo Fernandes [UNESP]
Chagas, Ana Carolina de Souza
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Federal University of Pampa
dc.contributor.author.fl_str_mv Figueiredo, Amanda [UNESP]
Anholeto, Luís Adriano
Cola, Diego Faria [UNESP]
Fantatto, Rafaela Regina [UNESP]
Gainza, Yousmel Alemán [UNESP]
dos Santos, Isabella Barbosa [UNESP]
Viçozzi, Gabriel Pedroso
Ávila, Daiana Silva
Fraceto, Leonardo Fernandes [UNESP]
Chagas, Ana Carolina de Souza
dc.subject.por.fl_str_mv Acaricide residues
Nanocarrier
Nanoformulations
Resistance
Tick control
topic Acaricide residues
Nanocarrier
Nanoformulations
Resistance
Tick control
description Nanoformulations containing zein nanoparticles (ZN) can promote the stability and protection of molecules with acaricidal activity. The present study sought to develop nanoformulations with ZN associated with cypermethrin (CYPE) + chlorpyrifos (CHLO) + a plant compound (citral, menthol or limonene), characterize them, and verify their efficacy against Rhipicephalus microplus ticks. Additionally, we aimed to assess its safety in nontarget nematodes found in soil at a site subjected to contamination by acaricides. The nanoformulations were characterized by dynamic light scattering and nanoparticle tracking analysis. Nanoformulations 1 (ZN+CYPE+CHLO+citral), 2 (ZN+CYPE+CHLO+menthol), and 3 (ZN+CYPE+CHLO+limonene) were measured for diameter, polydispersion, zeta potential, concentration, and encapsulation efficiency. Nanoformulations 1, 2, and 3 were evaluated in a range from 0.004 to 0.466 mg/mL on R. microplus larvae and caused mortality > 80% at concentrations above 0.029 mg/mL. The commercial acaricide Colosso® (CYPE 15 g + CHLO 25 g + citronellal 1 g) was evaluated also from 0.004 to 0.512 mg/mL and resulted in 71.9% larval mortality at 0.064 mg/mL. Formulations 1, 2, and 3 at 0.466 mg/mL showed acaricidal efficacy of 50.2%, 40.5%, and 60.1% on engorged females, respectively, while Colosso® at 0.512 mg/mL obtained only 39.4%. The nanoformulations exhibited long residual period of activity and lower toxicity to nontarget nematodes. ZN was able to protect the active compounds against degradation during the storage period. Thus, ZN can be an alternative for the development of new acaricidal formulations using lower concentrations of active compounds.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:10:48Z
2023-07-29T16:10:48Z
2023-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2023.109918
Veterinary Parasitology, v. 318.
1873-2550
0304-4017
http://hdl.handle.net/11449/249845
10.1016/j.vetpar.2023.109918
2-s2.0-85152097988
url http://dx.doi.org/10.1016/j.vetpar.2023.109918
http://hdl.handle.net/11449/249845
identifier_str_mv Veterinary Parasitology, v. 318.
1873-2550
0304-4017
10.1016/j.vetpar.2023.109918
2-s2.0-85152097988
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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