Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.biomedcentral.com/1471-2180/14/314 http://hdl.handle.net/11449/130300 |
Resumo: | Background: Forestomach fermentation in Australian marsupials such as wallabies and kangaroos, though analogous to rumen fermentation, results in lower methane emissions. Insights into hydrogenotrophy in these systems could help in devising strategies to reduce ruminal methanogenesis. Reductive acetogenesis may be a significant hydrogen sink in these systems and previous molecular analyses have revealed a novel diversity of putative acetogens in the tammar wallaby forestomach.Results: Methanogen-inhibited enrichment cultures prepared from tammar wallaby forestomach contents consumed hydrogen and produced primarily acetate. Functional gene (formyltetrahydrofolate synthetase and acetyl-CoA synthase) analyses revealed a restricted diversity of Clostridiales species as the putative acetogens in the cultures. A new acetogen (growth on H-2/CO2 with acetate as primary end product) designated isolate TWA4, was obtained from the cultures. Isolate TWA4 classified within the Lachnospiraceae and demonstrated > 97% rrs identity to previously isolated kangaroo acetogens. Isolate TWA4 was a potent hydrogenotroph and demonstrated excellent mixotrophic growth (concomitant consumption of hydrogen during heterotrophic growth) with glycerol. Mixotrophic growth of isolate TWA4 on glycerol resulted in increased cell densities and acetate production compared to autotrophic growth. Co-cultures with an autotrophic methanogen Methanobrevibacter smithii revealed that isolate TWA4 performed reductive acetogenesis under high hydrogen concentration (> 5 mM), but not at low concentrations. Under heterotrophic growth conditions, isolate TWA4 did not significantly stimulate methanogenesis in a co-culture with M. smithii contrary to the expectation for organisms growing fermentatively.Conclusions: The unique properties of tammar wallaby acetogens might be contributing factors to reduced methanogen numbers and methane emissions from tammar wallaby forestomach fermentation, compared to ruminal fermentation. The macropod forestomach may be a useful source of acetogens for future strategies to reduce methane emissions from ruminants, particularly if these strategies also include some level of methane suppression and/or acetogen stimulation, for example by harnessing mixotrophic growth capabilities |
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Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomachAcetogenAcetogenesisTammar wallabyRumenMethanogenesisBackground: Forestomach fermentation in Australian marsupials such as wallabies and kangaroos, though analogous to rumen fermentation, results in lower methane emissions. Insights into hydrogenotrophy in these systems could help in devising strategies to reduce ruminal methanogenesis. Reductive acetogenesis may be a significant hydrogen sink in these systems and previous molecular analyses have revealed a novel diversity of putative acetogens in the tammar wallaby forestomach.Results: Methanogen-inhibited enrichment cultures prepared from tammar wallaby forestomach contents consumed hydrogen and produced primarily acetate. Functional gene (formyltetrahydrofolate synthetase and acetyl-CoA synthase) analyses revealed a restricted diversity of Clostridiales species as the putative acetogens in the cultures. A new acetogen (growth on H-2/CO2 with acetate as primary end product) designated isolate TWA4, was obtained from the cultures. Isolate TWA4 classified within the Lachnospiraceae and demonstrated > 97% rrs identity to previously isolated kangaroo acetogens. Isolate TWA4 was a potent hydrogenotroph and demonstrated excellent mixotrophic growth (concomitant consumption of hydrogen during heterotrophic growth) with glycerol. Mixotrophic growth of isolate TWA4 on glycerol resulted in increased cell densities and acetate production compared to autotrophic growth. Co-cultures with an autotrophic methanogen Methanobrevibacter smithii revealed that isolate TWA4 performed reductive acetogenesis under high hydrogen concentration (> 5 mM), but not at low concentrations. Under heterotrophic growth conditions, isolate TWA4 did not significantly stimulate methanogenesis in a co-culture with M. smithii contrary to the expectation for organisms growing fermentatively.Conclusions: The unique properties of tammar wallaby acetogens might be contributing factors to reduced methanogen numbers and methane emissions from tammar wallaby forestomach fermentation, compared to ruminal fermentation. The macropod forestomach may be a useful source of acetogens for future strategies to reduce methane emissions from ruminants, particularly if these strategies also include some level of methane suppression and/or acetogen stimulation, for example by harnessing mixotrophic growth capabilitiesUniversity of QueenslandCSIRO Livestock IndustriesMeat and Livestock AustraliaAustralian Government Department of Agriculture, Fisheries and Forestry Carbon Farming Futures Filling the Research Gap Programformer Australian Greenhouse OfficeCSIRO Agr, St Lucia, Qld, AustraliaUniv Queensland, Sch Agr &Food Sci, Gatton, AustraliaZhejiang Univ, Minist Educ, Key Lab Mol Anim Nutr, Hangzhou 310003, Zhejiang, Peoples R ChinaUniv Estadual Paulista, Fac Agr &Vet Sci, Sao Paulo, BrazilUniv Queensland, Ctr Microscopy &Microanal, St Lucia, Qld, AustraliaUniv Estadual Paulista, Fac Agr &Vet Sci, Sao Paulo, BrazilBiomed Central LtdCSIRO AgrUniv QueenslandZhejiang UnivUniversidade Estadual Paulista (Unesp)Gagen, Emma J.Wang, JiakunPadmanabha, JagadishLiu, JingCarvalho de Carvalho, Isabela Pena [UNESP]Liu, JianxinWebb, Richard I.Al Jassim, RafatMorrison, MarkDenman, Stuart E.McSweeney, Christopher S.2015-11-03T18:06:41Z2015-11-03T18:06:41Z2014-12-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-14application/pdfhttp://www.biomedcentral.com/1471-2180/14/314Bmc Microbiology. London: Biomed Central Ltd, v. 14, 14 p., 2014.1471-2180http://hdl.handle.net/11449/13030010.1186/s12866-014-0314-3WOS:000349048300001WOS000349048300001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Microbiology2.8291,242info:eu-repo/semantics/openAccess2023-10-04T06:02:00Zoai:repositorio.unesp.br:11449/130300Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-04T06:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
title |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
spellingShingle |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach Gagen, Emma J. Acetogen Acetogenesis Tammar wallaby Rumen Methanogenesis |
title_short |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
title_full |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
title_fullStr |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
title_full_unstemmed |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
title_sort |
Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach |
author |
Gagen, Emma J. |
author_facet |
Gagen, Emma J. Wang, Jiakun Padmanabha, Jagadish Liu, Jing Carvalho de Carvalho, Isabela Pena [UNESP] Liu, Jianxin Webb, Richard I. Al Jassim, Rafat Morrison, Mark Denman, Stuart E. McSweeney, Christopher S. |
author_role |
author |
author2 |
Wang, Jiakun Padmanabha, Jagadish Liu, Jing Carvalho de Carvalho, Isabela Pena [UNESP] Liu, Jianxin Webb, Richard I. Al Jassim, Rafat Morrison, Mark Denman, Stuart E. McSweeney, Christopher S. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
CSIRO Agr Univ Queensland Zhejiang Univ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gagen, Emma J. Wang, Jiakun Padmanabha, Jagadish Liu, Jing Carvalho de Carvalho, Isabela Pena [UNESP] Liu, Jianxin Webb, Richard I. Al Jassim, Rafat Morrison, Mark Denman, Stuart E. McSweeney, Christopher S. |
dc.subject.por.fl_str_mv |
Acetogen Acetogenesis Tammar wallaby Rumen Methanogenesis |
topic |
Acetogen Acetogenesis Tammar wallaby Rumen Methanogenesis |
description |
Background: Forestomach fermentation in Australian marsupials such as wallabies and kangaroos, though analogous to rumen fermentation, results in lower methane emissions. Insights into hydrogenotrophy in these systems could help in devising strategies to reduce ruminal methanogenesis. Reductive acetogenesis may be a significant hydrogen sink in these systems and previous molecular analyses have revealed a novel diversity of putative acetogens in the tammar wallaby forestomach.Results: Methanogen-inhibited enrichment cultures prepared from tammar wallaby forestomach contents consumed hydrogen and produced primarily acetate. Functional gene (formyltetrahydrofolate synthetase and acetyl-CoA synthase) analyses revealed a restricted diversity of Clostridiales species as the putative acetogens in the cultures. A new acetogen (growth on H-2/CO2 with acetate as primary end product) designated isolate TWA4, was obtained from the cultures. Isolate TWA4 classified within the Lachnospiraceae and demonstrated > 97% rrs identity to previously isolated kangaroo acetogens. Isolate TWA4 was a potent hydrogenotroph and demonstrated excellent mixotrophic growth (concomitant consumption of hydrogen during heterotrophic growth) with glycerol. Mixotrophic growth of isolate TWA4 on glycerol resulted in increased cell densities and acetate production compared to autotrophic growth. Co-cultures with an autotrophic methanogen Methanobrevibacter smithii revealed that isolate TWA4 performed reductive acetogenesis under high hydrogen concentration (> 5 mM), but not at low concentrations. Under heterotrophic growth conditions, isolate TWA4 did not significantly stimulate methanogenesis in a co-culture with M. smithii contrary to the expectation for organisms growing fermentatively.Conclusions: The unique properties of tammar wallaby acetogens might be contributing factors to reduced methanogen numbers and methane emissions from tammar wallaby forestomach fermentation, compared to ruminal fermentation. The macropod forestomach may be a useful source of acetogens for future strategies to reduce methane emissions from ruminants, particularly if these strategies also include some level of methane suppression and/or acetogen stimulation, for example by harnessing mixotrophic growth capabilities |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-11 2015-11-03T18:06:41Z 2015-11-03T18:06:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.biomedcentral.com/1471-2180/14/314 Bmc Microbiology. London: Biomed Central Ltd, v. 14, 14 p., 2014. 1471-2180 http://hdl.handle.net/11449/130300 10.1186/s12866-014-0314-3 WOS:000349048300001 WOS000349048300001.pdf |
url |
http://www.biomedcentral.com/1471-2180/14/314 http://hdl.handle.net/11449/130300 |
identifier_str_mv |
Bmc Microbiology. London: Biomed Central Ltd, v. 14, 14 p., 2014. 1471-2180 10.1186/s12866-014-0314-3 WOS:000349048300001 WOS000349048300001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Microbiology 2.829 1,242 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-14 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964424852733952 |