Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1413-70542013000400008 http://hdl.handle.net/11449/75850 |
Resumo: | A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats. |
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Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratosAnnona crassiflora mart. Fruit pulp effects on biochemical parameters and rat colon carcinogenesisAberrant crypt fociBrazilian savannahDimethylhydrazineImmunohistochemistryOxidative stressA. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats.Universidade Federal de Alfenas/UNIFAL Gabriel Monteiro da Silva, Alfenas - MGUniversidade Federal de Alfenas/UNIFAL Instituto de Ciências Exatas, Alfenas - MGUniversidade Estadual Paulista 'Júlio de Mesquita Filho'/UNESP Instituto de Biociências de Botucatu, Botucatu - SPUniversidade Federal de Alfenas/UNIFAL, Alfenas - MGUniversidade Estadual Paulista 'Júlio de Mesquita Filho'/UNESP Instituto de Biociências de Botucatu, Botucatu - SPGabriel Monteiro da SilvaInstituto de Ciências ExatasUniversidade Estadual Paulista (Unesp)Universidade Federal de Alfenas (UNIFAL)Venâncio, Vinícius PaulaFerreira, Eric BatistaBrigagão, Maísa Ribeiro Pereira LimaPaula, Fernanda Borges de AraújoBarbisan, Luis Fernando [UNESP]Azevedo, Luciana2014-05-27T11:29:53Z2014-05-27T11:29:53Z2013-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article343-349application/pdfhttp://dx.doi.org/10.1590/S1413-70542013000400008Ciencia e Agrotecnologia, v. 37, n. 4, p. 343-349, 2013.1413-70541981-1829http://hdl.handle.net/11449/7585010.1590/S1413-70542013000400008S1413-70542013000400008WOS:0003237237000082-s2.0-848833513102-s2.0-84883351310.pdf3278528112652257Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporCiência e Agrotecnologia0.6720,383info:eu-repo/semantics/openAccess2023-12-08T06:17:33Zoai:repositorio.unesp.br:11449/75850Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-08T06:17:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos Annona crassiflora mart. Fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
title |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos |
spellingShingle |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos Venâncio, Vinícius Paula Aberrant crypt foci Brazilian savannah Dimethylhydrazine Immunohistochemistry Oxidative stress |
title_short |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos |
title_full |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos |
title_fullStr |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos |
title_full_unstemmed |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos |
title_sort |
Efeitos da polpa do fruto de annona crassiflora mart., nos parâmetros bioquímicos e na carcinogênese de colon de ratos |
author |
Venâncio, Vinícius Paula |
author_facet |
Venâncio, Vinícius Paula Ferreira, Eric Batista Brigagão, Maísa Ribeiro Pereira Lima Paula, Fernanda Borges de Araújo Barbisan, Luis Fernando [UNESP] Azevedo, Luciana |
author_role |
author |
author2 |
Ferreira, Eric Batista Brigagão, Maísa Ribeiro Pereira Lima Paula, Fernanda Borges de Araújo Barbisan, Luis Fernando [UNESP] Azevedo, Luciana |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Gabriel Monteiro da Silva Instituto de Ciências Exatas Universidade Estadual Paulista (Unesp) Universidade Federal de Alfenas (UNIFAL) |
dc.contributor.author.fl_str_mv |
Venâncio, Vinícius Paula Ferreira, Eric Batista Brigagão, Maísa Ribeiro Pereira Lima Paula, Fernanda Borges de Araújo Barbisan, Luis Fernando [UNESP] Azevedo, Luciana |
dc.subject.por.fl_str_mv |
Aberrant crypt foci Brazilian savannah Dimethylhydrazine Immunohistochemistry Oxidative stress |
topic |
Aberrant crypt foci Brazilian savannah Dimethylhydrazine Immunohistochemistry Oxidative stress |
description |
A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01 2014-05-27T11:29:53Z 2014-05-27T11:29:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1413-70542013000400008 Ciencia e Agrotecnologia, v. 37, n. 4, p. 343-349, 2013. 1413-7054 1981-1829 http://hdl.handle.net/11449/75850 10.1590/S1413-70542013000400008 S1413-70542013000400008 WOS:000323723700008 2-s2.0-84883351310 2-s2.0-84883351310.pdf 3278528112652257 |
url |
http://dx.doi.org/10.1590/S1413-70542013000400008 http://hdl.handle.net/11449/75850 |
identifier_str_mv |
Ciencia e Agrotecnologia, v. 37, n. 4, p. 343-349, 2013. 1413-7054 1981-1829 10.1590/S1413-70542013000400008 S1413-70542013000400008 WOS:000323723700008 2-s2.0-84883351310 2-s2.0-84883351310.pdf 3278528112652257 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Ciência e Agrotecnologia 0.672 0,383 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
343-349 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965209004081152 |