Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10695-016-0267-6 http://hdl.handle.net/11449/178211 |
Resumo: | This work describes gonadotropic (GtH) cells and their morphological and immunohistochemical changes during the spermatogenic cycle of Serrasalmus maculatus (continuous spermatogenesis) and Pimelodus maculatus (seasonal spermatogenesis). GtH cells, widely distributed in the proximal pars distalis of the adenohypophysis, were characterized as round-shaped cells with eccentric nucleus, and cytoplasm with basophilic secretory granules and a variable number of vacuoles for both species. Immunohistochemistry against β-follicle-stimulating hormone (Fsh) and β-luteinizing hormone (Lh) in adjacent sections showed two separated GtH-producing cell populations, and a third population where both GtHs are expressed in the same cell for both species. In the seasonal spermatogenesis of P. maculatus, GtH cells seemed to be more abundant during developing and spawning capable phases. In contrast, no cyclic changes were detected in the continuous spermatogenesis of S. maculatus, except for the strong immunoreaction for Fsh and Lh in males with intense spermiogenesis. We conclude that changes reported here might reflect the type of spermatogenic cycle (seasonal or continuous) which are under different regulatory mechanisms (environmental and internal cues) controlling the reproduction in these species. |
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Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical studyAdenohypophysisGonadotropinPituitary glandReproductionSpermatogenesisTeleosteiThis work describes gonadotropic (GtH) cells and their morphological and immunohistochemical changes during the spermatogenic cycle of Serrasalmus maculatus (continuous spermatogenesis) and Pimelodus maculatus (seasonal spermatogenesis). GtH cells, widely distributed in the proximal pars distalis of the adenohypophysis, were characterized as round-shaped cells with eccentric nucleus, and cytoplasm with basophilic secretory granules and a variable number of vacuoles for both species. Immunohistochemistry against β-follicle-stimulating hormone (Fsh) and β-luteinizing hormone (Lh) in adjacent sections showed two separated GtH-producing cell populations, and a third population where both GtHs are expressed in the same cell for both species. In the seasonal spermatogenesis of P. maculatus, GtH cells seemed to be more abundant during developing and spawning capable phases. In contrast, no cyclic changes were detected in the continuous spermatogenesis of S. maculatus, except for the strong immunoreaction for Fsh and Lh in males with intense spermiogenesis. We conclude that changes reported here might reflect the type of spermatogenic cycle (seasonal or continuous) which are under different regulatory mechanisms (environmental and internal cues) controlling the reproduction in these species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Morphology Institute of Biosciences São Paulo State University UNESPDepartment of Cell and Developmental Biology Institute of Biomedical Sciences University of São Paulo USPDepartment of Physiology Institute of Biosciences University of São Paulo USPDepartamento de Biologia Celular e do Desenvolvimento Instituto de Ciências Biomédicas Universidade de São Paulo, Av. Professor Lineu Prestes 1524Department of Morphology Institute of Biosciences São Paulo State University UNESPFAPESP: 2003/11078-9Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Nóbrega, Rafael Henrique [UNESP]de Jesus, Lázaro Wender OliveiraHonji, Renato MassaakiBorella, Maria Inês2018-12-11T17:29:20Z2018-12-11T17:29:20Z2017-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51-63application/pdfhttp://dx.doi.org/10.1007/s10695-016-0267-6Fish Physiology and Biochemistry, v. 43, n. 1, p. 51-63, 2017.1573-51680920-1742http://hdl.handle.net/11449/17821110.1007/s10695-016-0267-62-s2.0-849829657842-s2.0-84982965784.pdf05157085852539850000-0001-9796-5076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish Physiology and Biochemistry0,6900,690info:eu-repo/semantics/openAccess2024-01-01T06:23:10Zoai:repositorio.unesp.br:11449/178211Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-01T06:23:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
title |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
spellingShingle |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study Nóbrega, Rafael Henrique [UNESP] Adenohypophysis Gonadotropin Pituitary gland Reproduction Spermatogenesis Teleostei |
title_short |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
title_full |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
title_fullStr |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
title_full_unstemmed |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
title_sort |
Characterization of gonadotropic cells during continuous and seasonal spermatogenesis of two freshwater fish species: a histochemical and immunohistochemical study |
author |
Nóbrega, Rafael Henrique [UNESP] |
author_facet |
Nóbrega, Rafael Henrique [UNESP] de Jesus, Lázaro Wender Oliveira Honji, Renato Massaaki Borella, Maria Inês |
author_role |
author |
author2 |
de Jesus, Lázaro Wender Oliveira Honji, Renato Massaaki Borella, Maria Inês |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nóbrega, Rafael Henrique [UNESP] de Jesus, Lázaro Wender Oliveira Honji, Renato Massaaki Borella, Maria Inês |
dc.subject.por.fl_str_mv |
Adenohypophysis Gonadotropin Pituitary gland Reproduction Spermatogenesis Teleostei |
topic |
Adenohypophysis Gonadotropin Pituitary gland Reproduction Spermatogenesis Teleostei |
description |
This work describes gonadotropic (GtH) cells and their morphological and immunohistochemical changes during the spermatogenic cycle of Serrasalmus maculatus (continuous spermatogenesis) and Pimelodus maculatus (seasonal spermatogenesis). GtH cells, widely distributed in the proximal pars distalis of the adenohypophysis, were characterized as round-shaped cells with eccentric nucleus, and cytoplasm with basophilic secretory granules and a variable number of vacuoles for both species. Immunohistochemistry against β-follicle-stimulating hormone (Fsh) and β-luteinizing hormone (Lh) in adjacent sections showed two separated GtH-producing cell populations, and a third population where both GtHs are expressed in the same cell for both species. In the seasonal spermatogenesis of P. maculatus, GtH cells seemed to be more abundant during developing and spawning capable phases. In contrast, no cyclic changes were detected in the continuous spermatogenesis of S. maculatus, except for the strong immunoreaction for Fsh and Lh in males with intense spermiogenesis. We conclude that changes reported here might reflect the type of spermatogenic cycle (seasonal or continuous) which are under different regulatory mechanisms (environmental and internal cues) controlling the reproduction in these species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 2018-12-11T17:29:20Z 2018-12-11T17:29:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10695-016-0267-6 Fish Physiology and Biochemistry, v. 43, n. 1, p. 51-63, 2017. 1573-5168 0920-1742 http://hdl.handle.net/11449/178211 10.1007/s10695-016-0267-6 2-s2.0-84982965784 2-s2.0-84982965784.pdf 0515708585253985 0000-0001-9796-5076 |
url |
http://dx.doi.org/10.1007/s10695-016-0267-6 http://hdl.handle.net/11449/178211 |
identifier_str_mv |
Fish Physiology and Biochemistry, v. 43, n. 1, p. 51-63, 2017. 1573-5168 0920-1742 10.1007/s10695-016-0267-6 2-s2.0-84982965784 2-s2.0-84982965784.pdf 0515708585253985 0000-0001-9796-5076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish Physiology and Biochemistry 0,690 0,690 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
51-63 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965482900520960 |