Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1590/1678-4162-12214 http://hdl.handle.net/11449/212667 |
Resumo: | The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments. |
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Allogenic platelet-rich plasma in induced ulcers in rat’s cornea[Plasma rico em plaquetas alogênico em úlceras de córnea induzidas em ratos]heated platelet-rich plasmagrowth factorPDGFplasma rich in growth factorseye dropsplasma rico em plaquetas aquecidofator de crescimentoPDGFplasma rico em fatores de crescimentocolíriosThe objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.Objetivou-se avaliar o efeito clínico da aplicação de plasma rico em plaquetas alogênico (PRP) aquecido ou não, no tratamento de úlceras de córnea, como a dosagem de PDGF-BB na córnea. As úlceras foram induzidas, padronizando-se o olho esquerdo de 81 ratos (Rattus norvegicus, variedade albinus), aleatoriamente, nos três grupos (N = 27): grupo controle (CG), que não recebeu nenhum tratamento tópico; grupo PRP aquecido (GA) e grupo PRP (GP), que receberam tratamento tópico a cada oito horas, durante cinco dias. Cada grupo foi subdividido em 24 horas (M1), três dias (M3) e cinco dias (M5). O exame clínico avaliou a opacidade, a vascularização e o reparo corneano. O PDGF-BB corneano foi dosado pelo método Elisa. Houve diminuição da opacidade da córnea nos animais tratados com PRP (GA e GP) e diminuição do tempo de reparo da córnea em comparação com CG, M1 e M5. Além disso, foi observada maior vascularização no GP no momento M3 em relação ao M1. A aplicação de colírios de PRP alogênico, aquecidos ou não, acelera a cicatrização da córnea, além de reduzir o tempo de reparo da córnea. No entanto, a concentração de PDGF na córnea não se alterou em nenhum dos tratamentos.Universidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Instituto de BiociênciasUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Instituto de BiociênciasUniversidade Federal de Minas Gerais, Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Gandolfi, M.g. [UNESP]Brandão, C.v.s. [UNESP]Pellizon, C.h. [UNESP]Hirota, I.n. [UNESP]Barros, R. [UNESP]Hussein, A.c.b. [UNESP]Merlini, N.b. [UNESP]Takahira, R.k. [UNESP]Mesquita, L.r. [UNESP]2021-07-14T10:43:25Z2021-07-14T10:43:25Z2021-06-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article613-621application/pdfhttp://dx.doi.org/10.1590/1678-4162-12214Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 73, n. 03, p. 613-621, 2021.0102-09351678-4162http://hdl.handle.net/11449/21266710.1590/1678-4162-12214S0102-09352021000300613S0102-09352021000300613.pdf10876153890136550000-0002-2011-5214SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecniainfo:eu-repo/semantics/openAccess2023-10-07T06:04:34Zoai:repositorio.unesp.br:11449/212667Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-07T06:04:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea [Plasma rico em plaquetas alogênico em úlceras de córnea induzidas em ratos] |
| title |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea |
| spellingShingle |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea Gandolfi, M.g. [UNESP] heated platelet-rich plasma growth factor PDGF plasma rich in growth factors eye drops plasma rico em plaquetas aquecido fator de crescimento PDGF plasma rico em fatores de crescimento colírios |
| title_short |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea |
| title_full |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea |
| title_fullStr |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea |
| title_full_unstemmed |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea |
| title_sort |
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea |
| author |
Gandolfi, M.g. [UNESP] |
| author_facet |
Gandolfi, M.g. [UNESP] Brandão, C.v.s. [UNESP] Pellizon, C.h. [UNESP] Hirota, I.n. [UNESP] Barros, R. [UNESP] Hussein, A.c.b. [UNESP] Merlini, N.b. [UNESP] Takahira, R.k. [UNESP] Mesquita, L.r. [UNESP] |
| author_role |
author |
| author2 |
Brandão, C.v.s. [UNESP] Pellizon, C.h. [UNESP] Hirota, I.n. [UNESP] Barros, R. [UNESP] Hussein, A.c.b. [UNESP] Merlini, N.b. [UNESP] Takahira, R.k. [UNESP] Mesquita, L.r. [UNESP] |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
| dc.contributor.author.fl_str_mv |
Gandolfi, M.g. [UNESP] Brandão, C.v.s. [UNESP] Pellizon, C.h. [UNESP] Hirota, I.n. [UNESP] Barros, R. [UNESP] Hussein, A.c.b. [UNESP] Merlini, N.b. [UNESP] Takahira, R.k. [UNESP] Mesquita, L.r. [UNESP] |
| dc.subject.por.fl_str_mv |
heated platelet-rich plasma growth factor PDGF plasma rich in growth factors eye drops plasma rico em plaquetas aquecido fator de crescimento PDGF plasma rico em fatores de crescimento colírios |
| topic |
heated platelet-rich plasma growth factor PDGF plasma rich in growth factors eye drops plasma rico em plaquetas aquecido fator de crescimento PDGF plasma rico em fatores de crescimento colírios |
| description |
The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07-14T10:43:25Z 2021-07-14T10:43:25Z 2021-06-21 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-12214 Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 73, n. 03, p. 613-621, 2021. 0102-0935 1678-4162 http://hdl.handle.net/11449/212667 10.1590/1678-4162-12214 S0102-09352021000300613 S0102-09352021000300613.pdf 1087615389013655 0000-0002-2011-5214 |
| url |
http://dx.doi.org/10.1590/1678-4162-12214 http://hdl.handle.net/11449/212667 |
| identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 73, n. 03, p. 613-621, 2021. 0102-0935 1678-4162 10.1590/1678-4162-12214 S0102-09352021000300613 S0102-09352021000300613.pdf 1087615389013655 0000-0002-2011-5214 |
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eng |
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eng |
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Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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info:eu-repo/semantics/openAccess |
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openAccess |
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613-621 application/pdf |
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Universidade Federal de Minas Gerais, Escola de Veterinária |
| publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
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SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1799964467667140608 |