Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://tede.upf.br/jspui/handle/tede/1594 |
Resumo: | Angiostrongylus costaricensis causes a clinical and anatomopathologic entity named abdominal angiostrongyliasis, and the southern region of Brazil is considered endemic for this pathology. This work aimed to assess the immune response of mice by measuring cytokines and pro-inflammatory cells, and by macro- and microscopic assessment of the lesions of infected animals. The experiment was conducted with 4 groups of infected male Swiss mice, whereas each group had 5 animals; the uninfected control group also consisted of 5 animals and had a 21-day follow-up. Infected animals received 10 third-stage larvae of A. costaricensis. During the experimental period, blood was collected from infected groups at days 3, 7, 14, and 21 through intracardiac puncture, and euthanasia was performed in the respective days. The animals of the control group were euthanized at day 3. Laboratory examinations such as complete blood count were used as assessment parameter of blood cells, and flow cytometry was used to determine and quantify the presence of serum cytokines and to relate the inflammatory process. No significant changes were observed in the complete blood count, and flow cytometry showed an increase in interleukins assessed from day 14. In the anatomopathological diagnosis, the macroscopic assessment of liver, lung, spleen, kidney, and intestine showed no changes. Histopathology showed eosinophilic reactions and/or inflammation in liver, spleen, and intestine (10/20); also granuloma in the intestine wall (1/20) of infected mice. The set of information obtained in this experiment proves that the inflammatory process of infected mice was observed at its initial stage, which may be suggested by complete blood count results. At day 14, histopathology showed intestinal and hepatic eosinophilia in 75% of animals. Interleukins (IL-12p70, IL-6, IL-10, MCP-1, TNF-α, and INF-ɤ) increased at day 21, concomitantly 100% of animals presented intestinal and hepatic eosinophilia. These results allow concluding that understanding mechanisms involving the inflammatory process through cytokines kinetics is related to pathogen defense. |
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Vieira, Maria Isabel Botelho40412300044http://lattes.cnpq.br/889551962619977831665414898http://lattes.cnpq.br/7117727416078314Silva Filho, José Roberto da2019-01-03T11:33:26Z2016-09-23SILVA FILHO, José Roberto da. Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss. 2016. 38 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2016.http://tede.upf.br/jspui/handle/tede/1594Angiostrongylus costaricensis causes a clinical and anatomopathologic entity named abdominal angiostrongyliasis, and the southern region of Brazil is considered endemic for this pathology. This work aimed to assess the immune response of mice by measuring cytokines and pro-inflammatory cells, and by macro- and microscopic assessment of the lesions of infected animals. The experiment was conducted with 4 groups of infected male Swiss mice, whereas each group had 5 animals; the uninfected control group also consisted of 5 animals and had a 21-day follow-up. Infected animals received 10 third-stage larvae of A. costaricensis. During the experimental period, blood was collected from infected groups at days 3, 7, 14, and 21 through intracardiac puncture, and euthanasia was performed in the respective days. The animals of the control group were euthanized at day 3. Laboratory examinations such as complete blood count were used as assessment parameter of blood cells, and flow cytometry was used to determine and quantify the presence of serum cytokines and to relate the inflammatory process. No significant changes were observed in the complete blood count, and flow cytometry showed an increase in interleukins assessed from day 14. In the anatomopathological diagnosis, the macroscopic assessment of liver, lung, spleen, kidney, and intestine showed no changes. Histopathology showed eosinophilic reactions and/or inflammation in liver, spleen, and intestine (10/20); also granuloma in the intestine wall (1/20) of infected mice. The set of information obtained in this experiment proves that the inflammatory process of infected mice was observed at its initial stage, which may be suggested by complete blood count results. At day 14, histopathology showed intestinal and hepatic eosinophilia in 75% of animals. Interleukins (IL-12p70, IL-6, IL-10, MCP-1, TNF-α, and INF-ɤ) increased at day 21, concomitantly 100% of animals presented intestinal and hepatic eosinophilia. These results allow concluding that understanding mechanisms involving the inflammatory process through cytokines kinetics is related to pathogen defense.O Angiostrongylus costaricensis causa uma doença clínica e anatomopatológica denominada angiostrongilíase abdominal e a região Sul do Brasil é considerada endêmica para esta patologia. Os objetivos do presente trabalho foram avaliar a resposta imune dos camundongos através da dosagem das citocinas e células pró-inflamatórias e avaliação macro e microscópica das lesões em animais infectados. O experimento foi conduzido com 4 grupos de camundongos Swiss machos, infectados, constituído cada grupo de 5 animais; o grupo controle não infectado também foi composto com 5 animais, e os mesmos foram acompanhados por 21 dias, período onde se dá início os sinais clínicos. Nos infectados foram administradas 10 larvas de terceiro estadio de A. costaricensis. Durante o período experimental nos dias 3, 7, 14 e 21 foram colhidos sangue através de punção intracardíaca dos grupos infectados e feita a eutanásia nos respectivos dias. Os animais do grupo controle foram eutanasiados no dia 3. Os exames laboratoriais, tais como hemograma que foi usado como parâmetro para avaliar as células sanguíneas; citometria de fluxo usada para determinar e quantificar a presença de citocinas no soro e relacionar com o processo inflamatório; alterações anatopatologica para identificar as lesões macro e microscópicas. O conjunto de informações obtidas pela citometria de fluxo e histopatologia evidenciam que o processo inflamatório nos camundongos infectados foi observado a partir do 14º dia. No diagnóstico anatomopatológico a avaliação macroscópica do fígado, pulmão, baço, rim e intestino não mostrou alterações. Na histopatologia foram observadas reações eosinofílicas e/ou inflamação no fígado, baço e intestino (10/20); granuloma na parede intestinal (1/20) em camundongos infectados. A partir dos resultados obtidos neste experimento ficou evidenciado que o processo inflamatório dos camundongos infectados foi observado na fase inicial o que pode ser sugerido pelo aumento das citocinas pro-inflamatórias a partir do 14 ° dia. Na histopatologia foram observados no 14º dia infiltrado eosinofílico intestinal e hepática em 75% dos animais. No 21º dia ocorreu o aumento das interleucinas (IL-12p70, IL-6, IL-10, MCP-1, TNF-¿ e INF-¿), concomitantemente, 100% dos animais apresentaram infiltrado eosinofílico intestinal e hepático. Estes resultados permitem concluir que a compreensão dos mecanismos que envolvem o processo inflamatório através da cinética de citocinas estão relacionados com a defesa contra o patógeno.Submitted by Mariana Freitas (marianafreitas@upf.br) on 2019-01-03T11:33:26Z No. of bitstreams: 1 2016JoseRobertodaSilvaFilho.pdf: 584677 bytes, checksum: cdbd59c3a96d9db590319557f066883a (MD5)Made available in DSpace on 2019-01-03T11:33:26Z (GMT). No. of bitstreams: 1 2016JoseRobertodaSilvaFilho.pdf: 584677 bytes, checksum: cdbd59c3a96d9db590319557f066883a (MD5) Previous issue date: 2016-09-23application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em BioexperimentaçãoUPFBrasilFaculdade de Agronomia e Medicina Veterinária – FAMVEpidemiologiaZoonosesCIENCIAS AGRARIAS::MEDICINA VETERINARIAAngiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos SwissAbdominal angiostrongyliasis: tissue and hematological inflammatory response in Swiss miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis79452006251700495950050060053202200503672799453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFLICENSElicense.txtlicense.txttext/plain; charset=utf-81940http://tede.upf.br:8080/jspui/bitstream/tede/1594/1/license.txte0faded76e3df80302a4a0fb3f2bb5f3MD51ORIGINAL2016JoseRobertodaSilvaFilho.pdf2016JoseRobertodaSilvaFilho.pdfapplication/pdf584677http://tede.upf.br:8080/jspui/bitstream/tede/1594/2/2016JoseRobertodaSilvaFilho.pdfcdbd59c3a96d9db590319557f066883aMD52tede/15942019-01-03 09:33:26.215oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2019-01-03T11:33:26Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
dc.title.alternative.eng.fl_str_mv |
Abdominal angiostrongyliasis: tissue and hematological inflammatory response in Swiss mice |
title |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
spellingShingle |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss Silva Filho, José Roberto da Epidemiologia Zoonoses CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
title_full |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
title_fullStr |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
title_full_unstemmed |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
title_sort |
Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss |
author |
Silva Filho, José Roberto da |
author_facet |
Silva Filho, José Roberto da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vieira, Maria Isabel Botelho |
dc.contributor.advisor1ID.fl_str_mv |
40412300044 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8895519626199778 |
dc.contributor.authorID.fl_str_mv |
31665414898 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7117727416078314 |
dc.contributor.author.fl_str_mv |
Silva Filho, José Roberto da |
contributor_str_mv |
Vieira, Maria Isabel Botelho |
dc.subject.por.fl_str_mv |
Epidemiologia Zoonoses |
topic |
Epidemiologia Zoonoses CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Angiostrongylus costaricensis causes a clinical and anatomopathologic entity named abdominal angiostrongyliasis, and the southern region of Brazil is considered endemic for this pathology. This work aimed to assess the immune response of mice by measuring cytokines and pro-inflammatory cells, and by macro- and microscopic assessment of the lesions of infected animals. The experiment was conducted with 4 groups of infected male Swiss mice, whereas each group had 5 animals; the uninfected control group also consisted of 5 animals and had a 21-day follow-up. Infected animals received 10 third-stage larvae of A. costaricensis. During the experimental period, blood was collected from infected groups at days 3, 7, 14, and 21 through intracardiac puncture, and euthanasia was performed in the respective days. The animals of the control group were euthanized at day 3. Laboratory examinations such as complete blood count were used as assessment parameter of blood cells, and flow cytometry was used to determine and quantify the presence of serum cytokines and to relate the inflammatory process. No significant changes were observed in the complete blood count, and flow cytometry showed an increase in interleukins assessed from day 14. In the anatomopathological diagnosis, the macroscopic assessment of liver, lung, spleen, kidney, and intestine showed no changes. Histopathology showed eosinophilic reactions and/or inflammation in liver, spleen, and intestine (10/20); also granuloma in the intestine wall (1/20) of infected mice. The set of information obtained in this experiment proves that the inflammatory process of infected mice was observed at its initial stage, which may be suggested by complete blood count results. At day 14, histopathology showed intestinal and hepatic eosinophilia in 75% of animals. Interleukins (IL-12p70, IL-6, IL-10, MCP-1, TNF-α, and INF-ɤ) increased at day 21, concomitantly 100% of animals presented intestinal and hepatic eosinophilia. These results allow concluding that understanding mechanisms involving the inflammatory process through cytokines kinetics is related to pathogen defense. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-09-23 |
dc.date.accessioned.fl_str_mv |
2019-01-03T11:33:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA FILHO, José Roberto da. Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss. 2016. 38 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2016. |
dc.identifier.uri.fl_str_mv |
http://tede.upf.br/jspui/handle/tede/1594 |
identifier_str_mv |
SILVA FILHO, José Roberto da. Angiostrongilíase abdominal: resposta inflamatória tecidual e hematológica em camundongos Swiss. 2016. 38 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2016. |
url |
http://tede.upf.br/jspui/handle/tede/1594 |
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por |
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por |
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500 500 600 |
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453670264235017319 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade de Passo Fundo |
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Programa de Pós-Graduação em Bioexperimentação |
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UPF |
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Brasil |
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Faculdade de Agronomia e Medicina Veterinária – FAMV |
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Universidade de Passo Fundo |
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