Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://tede.upf.br/jspui/handle/tede/1597 |
Resumo: | This study, carried out at the Laboratory of Animal Pathology of the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo, deals with the analysis of 26 canine melanocytic neoplasms through clinical-pathological and immunohistochemical evaluation, with the objective of ascertaining the marking pattern of the β-catenin molecule and if there is a correlation with the cellular proliferation evaluated by Ki-67 expression, as well as if there is a correlation of these markers with clinical-pathological factors. Canine melanocytic neoplasms originate from the proliferation of melanocytes and are called melanocytoma when benign, and melanoma when malignant. Melanona present poor prognosis, causing metastases in most animals. The precise diagnosis is obtained through the association of clinical-pathological factors with immunohistochemistry, a necessary tool to establish the prognosis and appropriate therapy. Immunohistochemical markers, such as Ki-67, are used to improve the prognostic quality of human and canine melanoma, since they allow the evaluation of the rate of cell proliferation. This work described data on the clinical-pathological parameters of benign and malignant primary melanocytic neoplasms: cutaneous and oral, melanocytic and amelanotic. The variables were: tumor location, macroscopic ulceration, age and gender of the animals, as well as the presence of necrosis and microscopic ulceration, nuclear atypia and mitotic index. As for the labeling site of β-catenin, it was verified whether cytoplasmic, nuclear or mixed. The clinical-pathological parameters that demonstrated significance were: the age of affected animals, microscopic ulceration and necrosis, nuclear atypia and mitotic index, all in relation to melanomas. There was no statistical difference between the β-catenin labeling sites between benign or malignant melanocytic neoplasms, as well as no correlation between the β-catenin molecule and the Ki-67 marker. To date, the relationship of β-catenin expression and Ki-67 expression to pre-established clinical-pathological prognostic factors has not been studied. |
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Motta, Adriana Costa da69144621000http://lattes.cnpq.br/635644553723446300649889029http://lattes.cnpq.br/7409471835672865Machado, Tanise Policarpo2019-01-03T12:01:45Z2017-09-01MACHADO, Tanise Policarpo. Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas. 2017. 39 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2017.http://tede.upf.br/jspui/handle/tede/1597This study, carried out at the Laboratory of Animal Pathology of the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo, deals with the analysis of 26 canine melanocytic neoplasms through clinical-pathological and immunohistochemical evaluation, with the objective of ascertaining the marking pattern of the β-catenin molecule and if there is a correlation with the cellular proliferation evaluated by Ki-67 expression, as well as if there is a correlation of these markers with clinical-pathological factors. Canine melanocytic neoplasms originate from the proliferation of melanocytes and are called melanocytoma when benign, and melanoma when malignant. Melanona present poor prognosis, causing metastases in most animals. The precise diagnosis is obtained through the association of clinical-pathological factors with immunohistochemistry, a necessary tool to establish the prognosis and appropriate therapy. Immunohistochemical markers, such as Ki-67, are used to improve the prognostic quality of human and canine melanoma, since they allow the evaluation of the rate of cell proliferation. This work described data on the clinical-pathological parameters of benign and malignant primary melanocytic neoplasms: cutaneous and oral, melanocytic and amelanotic. The variables were: tumor location, macroscopic ulceration, age and gender of the animals, as well as the presence of necrosis and microscopic ulceration, nuclear atypia and mitotic index. As for the labeling site of β-catenin, it was verified whether cytoplasmic, nuclear or mixed. The clinical-pathological parameters that demonstrated significance were: the age of affected animals, microscopic ulceration and necrosis, nuclear atypia and mitotic index, all in relation to melanomas. There was no statistical difference between the β-catenin labeling sites between benign or malignant melanocytic neoplasms, as well as no correlation between the β-catenin molecule and the Ki-67 marker. To date, the relationship of β-catenin expression and Ki-67 expression to pre-established clinical-pathological prognostic factors has not been studied.Este estudo, realizado no Laboratório de Patologia Animal da Faculdade de Agronomia e Medicina Veterinária da Universidade de Passo Fundo, trata da análise de 26 neoplasias melanocíticas caninas através de avaliação clínico-patológica e imuno-histoquímica com o objetivo de averiguar o padrão de marcação da molécula ¿-catenina e se há correlação com a proliferação celular avaliada pela expressão do Ki-67, bem como se há correlação desses marcadores com os fatores clínico-patológicos. As neoplasias melanocíticas caninas originam-se da proliferação de melanócitos e são denominadas melanocitoma quando benignas e melanoma quando malignas. Melanomas apresentam mauprognóstico ocasionando metástases na maioria dos animais. O diagnóstico preciso é obtido através da associação dos fatores clínico-patológicos com a imuno-histoquímica, ferramenta necessária para estabelecer o prognóstico e a terapêutica adequada. Marcadores imuno-histoquímicos, como o Ki-67, são usados para melhorar a qualidade do prognóstico do melanoma humano e canino, pois possibilitam avaliar a taxa de proliferação celular. Este trabalho descreve dados referentes aos parâmetros clínico-patológicos das neoplasias melanocíticas primárias caninas benignas e malignas:cutâneas e orais, melanóticas e amelanóticas.Foram consideradas as variáveis:localização do tumor, ulceração macroscópica, idade e sexo dos animais, além da presença de necrose e ulceração microscópica, atipia nuclear e índice mitótico. Quanto ao local de marcação da ¿-catenina, foi verificado se citoplasmática, nuclear ou mista. Os parâmetros clínico-patológicos que demonstraram significância foram: a idade dos animais afetados, a ulceração microscópica e necrose, a atipia nuclear e o índice mitótico, todos em relação aos melanomas. Não houve diferença estatística entre os locais de marcação da ¿-catenina entre neoplasias melanociticas benignas ou malignas, bem como não foi observado correlação da molécula ¿-catenina e o marcador Ki-67. Até o presente, não tem sido estudada a relação da expressão da molécula ¿-catenina e a expressão do Ki-67 com os fatores prognósticos clínico-patológicos pré-estabelecidos.Submitted by Mariana Freitas (marianafreitas@upf.br) on 2019-01-03T12:01:45Z No. of bitstreams: 1 2017TanisePolicarpoMachado.pdf: 1138952 bytes, checksum: b4fed6731760fc97ad9468d188b632c4 (MD5)Made available in DSpace on 2019-01-03T12:01:45Z (GMT). No. of bitstreams: 1 2017TanisePolicarpoMachado.pdf: 1138952 bytes, checksum: b4fed6731760fc97ad9468d188b632c4 (MD5) Previous issue date: 2017-09-01application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em BioexperimentaçãoUPFBrasilFaculdade de Agronomia e Medicina Veterinária – FAMVMelanomaCâncerMarcadores biológicosTumoresAnimaisCIENCIAS AGRARIAS::MEDICINA VETERINARIAEstudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninasStudy of B-Catenin and KI-67 expression in canine melanocytic neoplasiasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis79452006251700495950050060053202200503672799453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFLICENSElicense.txtlicense.txttext/plain; charset=utf-81940http://tede.upf.br:8080/jspui/bitstream/tede/1597/1/license.txte0faded76e3df80302a4a0fb3f2bb5f3MD51ORIGINAL2017TanisePolicarpoMachado.pdf2017TanisePolicarpoMachado.pdfapplication/pdf1138952http://tede.upf.br:8080/jspui/bitstream/tede/1597/2/2017TanisePolicarpoMachado.pdfb4fed6731760fc97ad9468d188b632c4MD52tede/15972019-01-03 10:01:45.819oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2019-01-03T12:01:45Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
dc.title.alternative.eng.fl_str_mv |
Study of B-Catenin and KI-67 expression in canine melanocytic neoplasias |
title |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
spellingShingle |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas Machado, Tanise Policarpo Melanoma Câncer Marcadores biológicos Tumores Animais CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
title_full |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
title_fullStr |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
title_full_unstemmed |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
title_sort |
Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas |
author |
Machado, Tanise Policarpo |
author_facet |
Machado, Tanise Policarpo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Motta, Adriana Costa da |
dc.contributor.advisor1ID.fl_str_mv |
69144621000 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6356445537234463 |
dc.contributor.authorID.fl_str_mv |
00649889029 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7409471835672865 |
dc.contributor.author.fl_str_mv |
Machado, Tanise Policarpo |
contributor_str_mv |
Motta, Adriana Costa da |
dc.subject.por.fl_str_mv |
Melanoma Câncer Marcadores biológicos Tumores Animais |
topic |
Melanoma Câncer Marcadores biológicos Tumores Animais CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study, carried out at the Laboratory of Animal Pathology of the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo, deals with the analysis of 26 canine melanocytic neoplasms through clinical-pathological and immunohistochemical evaluation, with the objective of ascertaining the marking pattern of the β-catenin molecule and if there is a correlation with the cellular proliferation evaluated by Ki-67 expression, as well as if there is a correlation of these markers with clinical-pathological factors. Canine melanocytic neoplasms originate from the proliferation of melanocytes and are called melanocytoma when benign, and melanoma when malignant. Melanona present poor prognosis, causing metastases in most animals. The precise diagnosis is obtained through the association of clinical-pathological factors with immunohistochemistry, a necessary tool to establish the prognosis and appropriate therapy. Immunohistochemical markers, such as Ki-67, are used to improve the prognostic quality of human and canine melanoma, since they allow the evaluation of the rate of cell proliferation. This work described data on the clinical-pathological parameters of benign and malignant primary melanocytic neoplasms: cutaneous and oral, melanocytic and amelanotic. The variables were: tumor location, macroscopic ulceration, age and gender of the animals, as well as the presence of necrosis and microscopic ulceration, nuclear atypia and mitotic index. As for the labeling site of β-catenin, it was verified whether cytoplasmic, nuclear or mixed. The clinical-pathological parameters that demonstrated significance were: the age of affected animals, microscopic ulceration and necrosis, nuclear atypia and mitotic index, all in relation to melanomas. There was no statistical difference between the β-catenin labeling sites between benign or malignant melanocytic neoplasms, as well as no correlation between the β-catenin molecule and the Ki-67 marker. To date, the relationship of β-catenin expression and Ki-67 expression to pre-established clinical-pathological prognostic factors has not been studied. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-09-01 |
dc.date.accessioned.fl_str_mv |
2019-01-03T12:01:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MACHADO, Tanise Policarpo. Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas. 2017. 39 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2017. |
dc.identifier.uri.fl_str_mv |
http://tede.upf.br/jspui/handle/tede/1597 |
identifier_str_mv |
MACHADO, Tanise Policarpo. Estudo da expressão de B-Catenina e do KI-67 em neoplasias melanocíticas caninas. 2017. 39 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2017. |
url |
http://tede.upf.br/jspui/handle/tede/1597 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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794520062517004959 |
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500 500 600 |
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53202200503672799 |
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453670264235017319 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Bioexperimentação |
dc.publisher.initials.fl_str_mv |
UPF |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia e Medicina Veterinária – FAMV |
publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.source.none.fl_str_mv |
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