Estabilização e solidificação de Cromo (VI) por biocimentação
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://tede.upf.br/jspui/handle/tede/1520 |
Resumo: | Most of the soil improvement techniques used in the world involve the addition of mechanical energy and/or synthetic materials, both of which have substantial energy costs associated with their production. However, the technique of biocementation intend to improve the mechanical properties of soil in a sustainable way. Biocementation is the formation of particulate matter through the soil particles through the metabolism of microorganisms present. The numerous microbiological processes have the potential to modify soil behavior. Many bacteria like the species: Bacillus, Sporosarcina, Sporolactobacillus and Clostridium have potential of biological cementation. Thus, the objective of this work was to perform the precipitation of Calcium Carbonate induced by microorganisms or biocementation for the stabilization and solidification of hexavalent chromium (Cr(VI)) in a sandy soil. We perform tests of urease activity, biocementation test, soil and leachate analysis, and SEM. The tests were carried out in the environmental geotechnics laboratory of the University of Passo Fundo. In the first phase of the dissertation, in the test of non-sterilized urease activity, it was possible to observe the adaptation of the native microorganisms of the soil to the nutrient solutions added to the medium. Moreover, in the sterilized urease assay it was possible to observe that the activitymeasured in the absence of microorganisms is considered to be chemical. The zero time in both assays presented similar results, so it was possible to calculate the actual activity performed by microorganisms in the assay. It was also possible to observe that the nutrient solution C stood out with greater activity in the non-autoclaved test. The peak of activity in the time 10 days, being the nutrient solution used in the biocementation test to encapsulate hexavalent chromium in sandy soil, with two feeding intervals, 2 days as used in bibliography and 7 days, approaching the peak of urease found in the first phase of the dissertation. At the end of the second phase, it can be observed from the analysis carried out in the collected leachate that the contaminant retention capacity is higher by the nutrient medium fed specimens, approximately 30%. While the test bodies fed with water showed the lowest contaminant retention, approximately 10%. After that with the results of the soil analysis it was possible to observe that there was biocementation, however, a low concentration was retained in the biocement, thus affirming that in case of field application, the biocementation may not be effective, because in this study approximately 70% of the contaminant inserted in the molding was leached. We concluded with this study that the biocimentation for sandy soils is not efficient with percolation feeding, because the risk of spreading the plume of contamination is high, reducing the efficiency of the technique. |
id |
UPF-1_73a620a8e3a4b6d67dded5c9c57f2c05 |
---|---|
oai_identifier_str |
oai:tede.upf.br:tede/1520 |
network_acronym_str |
UPF-1 |
network_name_str |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
repository_id_str |
|
spelling |
Thomé, Antônio48314447072http://lattes.cnpq.br/252298543170272703370355043http://lattes.cnpq.br/1607707356154633Dall'Agnol, Bruna Bilhar2018-10-05T23:08:17Z2018-04-17DALL'AGNOL, Bruna Bilhar. Estabilização e solidificação de Cromo (VI) por biocimentação. 2018. 71 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2018.http://tede.upf.br/jspui/handle/tede/1520Most of the soil improvement techniques used in the world involve the addition of mechanical energy and/or synthetic materials, both of which have substantial energy costs associated with their production. However, the technique of biocementation intend to improve the mechanical properties of soil in a sustainable way. Biocementation is the formation of particulate matter through the soil particles through the metabolism of microorganisms present. The numerous microbiological processes have the potential to modify soil behavior. Many bacteria like the species: Bacillus, Sporosarcina, Sporolactobacillus and Clostridium have potential of biological cementation. Thus, the objective of this work was to perform the precipitation of Calcium Carbonate induced by microorganisms or biocementation for the stabilization and solidification of hexavalent chromium (Cr(VI)) in a sandy soil. We perform tests of urease activity, biocementation test, soil and leachate analysis, and SEM. The tests were carried out in the environmental geotechnics laboratory of the University of Passo Fundo. In the first phase of the dissertation, in the test of non-sterilized urease activity, it was possible to observe the adaptation of the native microorganisms of the soil to the nutrient solutions added to the medium. Moreover, in the sterilized urease assay it was possible to observe that the activitymeasured in the absence of microorganisms is considered to be chemical. The zero time in both assays presented similar results, so it was possible to calculate the actual activity performed by microorganisms in the assay. It was also possible to observe that the nutrient solution C stood out with greater activity in the non-autoclaved test. The peak of activity in the time 10 days, being the nutrient solution used in the biocementation test to encapsulate hexavalent chromium in sandy soil, with two feeding intervals, 2 days as used in bibliography and 7 days, approaching the peak of urease found in the first phase of the dissertation. At the end of the second phase, it can be observed from the analysis carried out in the collected leachate that the contaminant retention capacity is higher by the nutrient medium fed specimens, approximately 30%. While the test bodies fed with water showed the lowest contaminant retention, approximately 10%. After that with the results of the soil analysis it was possible to observe that there was biocementation, however, a low concentration was retained in the biocement, thus affirming that in case of field application, the biocementation may not be effective, because in this study approximately 70% of the contaminant inserted in the molding was leached. We concluded with this study that the biocimentation for sandy soils is not efficient with percolation feeding, because the risk of spreading the plume of contamination is high, reducing the efficiency of the technique.A maioria das técnicas de melhoramento de solo que são utilizadas no mundo envolve a adição de energia mecânica e/ou materiais sintéticos, sendo que ambos têm substancial custos de energia associados à sua produção. No entanto, a técnica de biocimentação vem como intuito de melhorar as propriedades mecânicas do solo de forma sustentável. A biocimentação é a formação de material particulado por entre as partículas do solo através do metabolismo de microrganismos presentes. Os inúmeros processos microbiológicos têm o potencial para modificar o comportamento do solo. Muitas bactérias como as espécies: Bacillus, Sporosarcina, Sporolactobacillus e Clostridium possuem potencial de cimentação biológica. Dessa forma, o objetivo deste trabalho foi realizar a precipitação de Carbonato de Cálcio induzida por microrganismos ou biocimentação para a estabilização e solidificação de cromo hexavalente (Cr(VI)) em um solo arenoso. Foram realizados ensaios de teste de atividade urease, ensaio de biocimentação, análise de solo e lixiviado, além de MEV. Os ensaios foram realizados no laboratório de geotecnia ambiental da Universidade de Passo Fundo. Na primeira fase da dissertação, no ensaio de teste de atividade urease não autoclavado foi possível observar a adaptação dos microrganismos nativos do solo às soluções nutrientes adicionadas ao meio. Além disso, no ensaio autoclavado da urease foi possível observar que a atividade pode ser medida na ausência de microrganismos, sendo considerada química. O tempo zero em ambos os ensaios apresentou resultados semelhantes, assim, foi possível calcular a real atividade realizada por microrganismos no ensaio. Foi possível observar também que a solução nutriente C se destacou com maior atividade urease no ensaio não autoclavado com pico de atividade no tempo 10 dias, sendo a utilizada no teste de biocimentação para encapsulamento de cromo hexavalente em solo arenoso, com dois intervalos de alimentação, 2 dias como utilizado em bibliografia e 7 dias, aproximando ao pico de urease encontrado na primeira fase da dissertação. Ao final do ensaio de biocimentação na segunda fase, pode-se observar através da análise realizada no lixiviado coletado que a capacidade de retenção de contaminante é maior pelos corpos de prova alimentados com meio nutriente, aproximadamente 30%, enquanto os corpos de prova alimentados com água apresentaram retenção do contaminante mais baixa, aproximadamente 10%, depois disso com os resultados da análise do solo foi possível observar que houve biocimentação, no entanto, uma baixa concentração ficou retida no biocimento, assim afirmando que em caso de aplicação em campo, a biocimentação pode não ser efetiva, pois nesse estudo aproximadamente 70% do contaminante inserido na moldagem foi lixiviado. Concluiu-se com esse estudo que a biocimentação para solos arenosos não é eficiente com alimentação por percolação, pois o risco de espalhar a pluma de contaminação é alto, reduzindo a eficiência da técnica.Submitted by Mariana Freitas (marianafreitas@upf.br) on 2018-10-05T23:08:17Z No. of bitstreams: 1 2018BrunaBilharDallagnol.pdf: 2785606 bytes, checksum: cff775a1426b532743892ee0c109b1e8 (MD5)Made available in DSpace on 2018-10-05T23:08:17Z (GMT). No. of bitstreams: 1 2018BrunaBilharDallagnol.pdf: 2785606 bytes, checksum: cff775a1426b532743892ee0c109b1e8 (MD5) Previous issue date: 2018-04-17application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em Engenharia Civil e AmbientalUPFBrasilFaculdade de Engenharia e Arquitetura – FEARMecânica do soloSolidificaçãoSolosCompactaçãoCromoENGENHARIAS::ENGENHARIA CIVILEstabilização e solidificação de Cromo (VI) por biocimentaçãoStabilization and solidification of chromium (VI) by biocimentationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-41729612957170071185005006008147033241558623806-6274833215046395772info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFLICENSElicense.txtlicense.txttext/plain; charset=utf-81940http://tede.upf.br:8080/jspui/bitstream/tede/1520/1/license.txte0faded76e3df80302a4a0fb3f2bb5f3MD51ORIGINAL2018BrunaBilharDallagnol.pdf2018BrunaBilharDallagnol.pdfapplication/pdf2785606http://tede.upf.br:8080/jspui/bitstream/tede/1520/2/2018BrunaBilharDallagnol.pdfcff775a1426b532743892ee0c109b1e8MD52tede/15202018-10-05 20:08:17.159oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2018-10-05T23:08:17Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Estabilização e solidificação de Cromo (VI) por biocimentação |
dc.title.alternative.eng.fl_str_mv |
Stabilization and solidification of chromium (VI) by biocimentation |
title |
Estabilização e solidificação de Cromo (VI) por biocimentação |
spellingShingle |
Estabilização e solidificação de Cromo (VI) por biocimentação Dall'Agnol, Bruna Bilhar Mecânica do solo Solidificação Solos Compactação Cromo ENGENHARIAS::ENGENHARIA CIVIL |
title_short |
Estabilização e solidificação de Cromo (VI) por biocimentação |
title_full |
Estabilização e solidificação de Cromo (VI) por biocimentação |
title_fullStr |
Estabilização e solidificação de Cromo (VI) por biocimentação |
title_full_unstemmed |
Estabilização e solidificação de Cromo (VI) por biocimentação |
title_sort |
Estabilização e solidificação de Cromo (VI) por biocimentação |
author |
Dall'Agnol, Bruna Bilhar |
author_facet |
Dall'Agnol, Bruna Bilhar |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Thomé, Antônio |
dc.contributor.advisor1ID.fl_str_mv |
48314447072 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2522985431702727 |
dc.contributor.authorID.fl_str_mv |
03370355043 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1607707356154633 |
dc.contributor.author.fl_str_mv |
Dall'Agnol, Bruna Bilhar |
contributor_str_mv |
Thomé, Antônio |
dc.subject.por.fl_str_mv |
Mecânica do solo Solidificação Solos Compactação Cromo |
topic |
Mecânica do solo Solidificação Solos Compactação Cromo ENGENHARIAS::ENGENHARIA CIVIL |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA CIVIL |
description |
Most of the soil improvement techniques used in the world involve the addition of mechanical energy and/or synthetic materials, both of which have substantial energy costs associated with their production. However, the technique of biocementation intend to improve the mechanical properties of soil in a sustainable way. Biocementation is the formation of particulate matter through the soil particles through the metabolism of microorganisms present. The numerous microbiological processes have the potential to modify soil behavior. Many bacteria like the species: Bacillus, Sporosarcina, Sporolactobacillus and Clostridium have potential of biological cementation. Thus, the objective of this work was to perform the precipitation of Calcium Carbonate induced by microorganisms or biocementation for the stabilization and solidification of hexavalent chromium (Cr(VI)) in a sandy soil. We perform tests of urease activity, biocementation test, soil and leachate analysis, and SEM. The tests were carried out in the environmental geotechnics laboratory of the University of Passo Fundo. In the first phase of the dissertation, in the test of non-sterilized urease activity, it was possible to observe the adaptation of the native microorganisms of the soil to the nutrient solutions added to the medium. Moreover, in the sterilized urease assay it was possible to observe that the activitymeasured in the absence of microorganisms is considered to be chemical. The zero time in both assays presented similar results, so it was possible to calculate the actual activity performed by microorganisms in the assay. It was also possible to observe that the nutrient solution C stood out with greater activity in the non-autoclaved test. The peak of activity in the time 10 days, being the nutrient solution used in the biocementation test to encapsulate hexavalent chromium in sandy soil, with two feeding intervals, 2 days as used in bibliography and 7 days, approaching the peak of urease found in the first phase of the dissertation. At the end of the second phase, it can be observed from the analysis carried out in the collected leachate that the contaminant retention capacity is higher by the nutrient medium fed specimens, approximately 30%. While the test bodies fed with water showed the lowest contaminant retention, approximately 10%. After that with the results of the soil analysis it was possible to observe that there was biocementation, however, a low concentration was retained in the biocement, thus affirming that in case of field application, the biocementation may not be effective, because in this study approximately 70% of the contaminant inserted in the molding was leached. We concluded with this study that the biocimentation for sandy soils is not efficient with percolation feeding, because the risk of spreading the plume of contamination is high, reducing the efficiency of the technique. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-10-05T23:08:17Z |
dc.date.issued.fl_str_mv |
2018-04-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DALL'AGNOL, Bruna Bilhar. Estabilização e solidificação de Cromo (VI) por biocimentação. 2018. 71 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede.upf.br/jspui/handle/tede/1520 |
identifier_str_mv |
DALL'AGNOL, Bruna Bilhar. Estabilização e solidificação de Cromo (VI) por biocimentação. 2018. 71 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2018. |
url |
http://tede.upf.br/jspui/handle/tede/1520 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-4172961295717007118 |
dc.relation.confidence.fl_str_mv |
500 500 600 |
dc.relation.department.fl_str_mv |
8147033241558623806 |
dc.relation.cnpq.fl_str_mv |
-6274833215046395772 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia Civil e Ambiental |
dc.publisher.initials.fl_str_mv |
UPF |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Engenharia e Arquitetura – FEAR |
publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.source.none.fl_str_mv |
reponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) instname:Universidade de Passo Fundo (UPF) instacron:UPF |
instname_str |
Universidade de Passo Fundo (UPF) |
instacron_str |
UPF |
institution |
UPF |
reponame_str |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
collection |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
bitstream.url.fl_str_mv |
http://tede.upf.br:8080/jspui/bitstream/tede/1520/1/license.txt http://tede.upf.br:8080/jspui/bitstream/tede/1520/2/2018BrunaBilharDallagnol.pdf |
bitstream.checksum.fl_str_mv |
e0faded76e3df80302a4a0fb3f2bb5f3 cff775a1426b532743892ee0c109b1e8 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF) |
repository.mail.fl_str_mv |
biblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.br |
_version_ |
1809092291734798336 |