Quirópteros: população e potencial zoonótico em granjas de suínos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://tede.upf.br/jspui/handle/tede/1595 |
Resumo: | This study evaluates the zoonotic potential of bats that use pig farms or their surroundings as lodgings for their colonies in southern Brazil, including rural areas of the municipalities of Camargo/RS, Vila Maria/RS and Concordia/SC also describes the identification of families found (Molossidae and Vespertilionidae) and also species (Molossus molossus, Histiotus velatus and Molossus rufus). As authorized by the national committee (SISBIO / ICMBIO) and the Ethics Committee on Animal Use at the University of Passo Fundo (CEUA-UPF), were captured 80 individuals, and they belong to two families and three species. In the same area blood samples were collected from 37 pigs in the nursery phase. All bats underwent capturing procedures through mist nets, inhalation sedation, transport procedures, anesthesia, identify families/species, collection of blood through cardiac puncture, euthanasia, necropsy, collecting tissues of interest (lung, and liver). After collecting tissues RNA extraction was done using Trizol®, of blood samples from bats and pigs, also bats lungs and liver. It was investigated in blood samples (bats and pigs) and lung (bats) or if there was not the presence of influenza virus by RT-PCR (reverse transcriptase reaction, followed by Polymerase Chain Reaction). Subsequently liver RNA samples were analyzed by nested RT-PCR and RT-PCR assays for hepatitis E virus from the cDNA synthesis obtaining only negative for these agents. The liver RNA samples were also evaluated for the presence of adenovirus by HRM assay. qPCR was performed in such a reaction, we obtained nonspecific amplifications in 35 samples, which were tested again with primers for different species of AdV resulting in 7 positive samples, which were sequenced and are shown in phylogenetic trees. |
id |
UPF-1_d2f6a9824cb3fb32ebf5cf7fb7445a2b |
---|---|
oai_identifier_str |
oai:tede.upf.br:tede/1595 |
network_acronym_str |
UPF-1 |
network_name_str |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
repository_id_str |
|
spelling |
Zanella, Eraldo Lourenso42764424000http://lattes.cnpq.br/922195344296416100842743014http://lattes.cnpq.br/7346465861267227Stefanello, Flávia2019-01-03T11:42:54Z2016-04-04STEFANELLO, Flávia. Quirópteros: população e potencial zoonótico em granjas de suínos. 2016. 69 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2016.http://tede.upf.br/jspui/handle/tede/1595This study evaluates the zoonotic potential of bats that use pig farms or their surroundings as lodgings for their colonies in southern Brazil, including rural areas of the municipalities of Camargo/RS, Vila Maria/RS and Concordia/SC also describes the identification of families found (Molossidae and Vespertilionidae) and also species (Molossus molossus, Histiotus velatus and Molossus rufus). As authorized by the national committee (SISBIO / ICMBIO) and the Ethics Committee on Animal Use at the University of Passo Fundo (CEUA-UPF), were captured 80 individuals, and they belong to two families and three species. In the same area blood samples were collected from 37 pigs in the nursery phase. All bats underwent capturing procedures through mist nets, inhalation sedation, transport procedures, anesthesia, identify families/species, collection of blood through cardiac puncture, euthanasia, necropsy, collecting tissues of interest (lung, and liver). After collecting tissues RNA extraction was done using Trizol®, of blood samples from bats and pigs, also bats lungs and liver. It was investigated in blood samples (bats and pigs) and lung (bats) or if there was not the presence of influenza virus by RT-PCR (reverse transcriptase reaction, followed by Polymerase Chain Reaction). Subsequently liver RNA samples were analyzed by nested RT-PCR and RT-PCR assays for hepatitis E virus from the cDNA synthesis obtaining only negative for these agents. The liver RNA samples were also evaluated for the presence of adenovirus by HRM assay. qPCR was performed in such a reaction, we obtained nonspecific amplifications in 35 samples, which were tested again with primers for different species of AdV resulting in 7 positive samples, which were sequenced and are shown in phylogenetic trees.O presente estudo avalia o potencial zoonótico de morcegos que utilizam granjas de suínos ou seu entorno como abrigo para suas colônias, na região Sul do Brasil, incluindo áreas rurais dos municípios de Camargo/RS, Vila Maria/RS e Concórdia/SC, além disso, descreve a identificação das famílias encontradas (Molossidae e Vespertilionidae) e também as espécies (Molossus molossus, Histiotus velatus e Molossus rufus). Conforme autorização do órgão nacional competente (SISBIO/ICMBIO) e a Comissão de Ética no Uso de Animais da Universidade de Passo Fundo (CEUA-UPF) capturamos 80 indivíduos, sendo os mesmos pertencentes a duas famílias e três espécies. Na mesma área foi coletado amostra de 37 suinos na fase de creche. Todos os animais passaram por procedimentos de captura através de redes de neblina, sedação inalatória, procedimentos de transporte, anestesia, identificação de famílias/espécies, coleta de sangue através de punção cardíaca, eutanásia, necropsia, coleta de tecidos de interesse (pulmão, fígado). Após a coleta de tecidos, realizou-se a extração de RNA, feita com Trizol®, de amostras de sangue de morcegos e de 37 suínos, pulmão e fígado de morcegos. Investigamos em amostras de sangue (morcegos e suínos) e pulmão (morcegos) se havia ou não a presença do vírus Influenza A, através de RT-PCR (reação da transcriptase reversa, seguida Reação da Polimerase em Cadeia). Posteriormente amostras de RNA de fígado, foram analisadas através de Nested-RT-PCR e ensaios de RT-PCR para o vírus da Hepatite E, a partir da síntese de cDNA obtendo apenas resultados negativos para tais agentes. As amostras de RNA de fígado também foram avaliadas quanto a presença de Adenovírus, através do ensaio HRM. Foi realizado qPCR, em tal reação, obtivemos amplificações inespecíficas em 35 amostras, as quais foram novamente testadas com primers através da técnica Nested PCR, para diferentes espécies de AdV, resultando em 7 amostras positivas, as quais foram sequenciadas e construída uma árvore filogenética.Submitted by Mariana Freitas (marianafreitas@upf.br) on 2019-01-03T11:42:54Z No. of bitstreams: 1 2015FlaviaStefanello.pdf: 1599964 bytes, checksum: 93289aef7cf6bb8d5ce63b126801995b (MD5)Made available in DSpace on 2019-01-03T11:42:54Z (GMT). No. of bitstreams: 1 2015FlaviaStefanello.pdf: 1599964 bytes, checksum: 93289aef7cf6bb8d5ce63b126801995b (MD5) Previous issue date: 2016-04-04application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em BioexperimentaçãoUPFBrasilFaculdade de Agronomia e Medicina Veterinária – FAMVMorcegoZoonosesInfluenzaSuínoCIENCIAS AGRARIAS::MEDICINA VETERINARIAQuirópteros: população e potencial zoonótico em granjas de suínosChiroptera: population and zoonotic potential in pig farmsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis79452006251700495950050060053202200503672799453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFLICENSElicense.txtlicense.txttext/plain; charset=utf-81940http://tede.upf.br:8080/jspui/bitstream/tede/1595/1/license.txte0faded76e3df80302a4a0fb3f2bb5f3MD51ORIGINAL2015FlaviaStefanello.pdf2015FlaviaStefanello.pdfapplication/pdf1599964http://tede.upf.br:8080/jspui/bitstream/tede/1595/2/2015FlaviaStefanello.pdf93289aef7cf6bb8d5ce63b126801995bMD52tede/15952019-01-03 09:42:54.942oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2019-01-03T11:42:54Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Quirópteros: população e potencial zoonótico em granjas de suínos |
dc.title.alternative.eng.fl_str_mv |
Chiroptera: population and zoonotic potential in pig farms |
title |
Quirópteros: população e potencial zoonótico em granjas de suínos |
spellingShingle |
Quirópteros: população e potencial zoonótico em granjas de suínos Stefanello, Flávia Morcego Zoonoses Influenza Suíno CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Quirópteros: população e potencial zoonótico em granjas de suínos |
title_full |
Quirópteros: população e potencial zoonótico em granjas de suínos |
title_fullStr |
Quirópteros: população e potencial zoonótico em granjas de suínos |
title_full_unstemmed |
Quirópteros: população e potencial zoonótico em granjas de suínos |
title_sort |
Quirópteros: população e potencial zoonótico em granjas de suínos |
author |
Stefanello, Flávia |
author_facet |
Stefanello, Flávia |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Zanella, Eraldo Lourenso |
dc.contributor.advisor1ID.fl_str_mv |
42764424000 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9221953442964161 |
dc.contributor.authorID.fl_str_mv |
00842743014 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7346465861267227 |
dc.contributor.author.fl_str_mv |
Stefanello, Flávia |
contributor_str_mv |
Zanella, Eraldo Lourenso |
dc.subject.por.fl_str_mv |
Morcego Zoonoses Influenza Suíno |
topic |
Morcego Zoonoses Influenza Suíno CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study evaluates the zoonotic potential of bats that use pig farms or their surroundings as lodgings for their colonies in southern Brazil, including rural areas of the municipalities of Camargo/RS, Vila Maria/RS and Concordia/SC also describes the identification of families found (Molossidae and Vespertilionidae) and also species (Molossus molossus, Histiotus velatus and Molossus rufus). As authorized by the national committee (SISBIO / ICMBIO) and the Ethics Committee on Animal Use at the University of Passo Fundo (CEUA-UPF), were captured 80 individuals, and they belong to two families and three species. In the same area blood samples were collected from 37 pigs in the nursery phase. All bats underwent capturing procedures through mist nets, inhalation sedation, transport procedures, anesthesia, identify families/species, collection of blood through cardiac puncture, euthanasia, necropsy, collecting tissues of interest (lung, and liver). After collecting tissues RNA extraction was done using Trizol®, of blood samples from bats and pigs, also bats lungs and liver. It was investigated in blood samples (bats and pigs) and lung (bats) or if there was not the presence of influenza virus by RT-PCR (reverse transcriptase reaction, followed by Polymerase Chain Reaction). Subsequently liver RNA samples were analyzed by nested RT-PCR and RT-PCR assays for hepatitis E virus from the cDNA synthesis obtaining only negative for these agents. The liver RNA samples were also evaluated for the presence of adenovirus by HRM assay. qPCR was performed in such a reaction, we obtained nonspecific amplifications in 35 samples, which were tested again with primers for different species of AdV resulting in 7 positive samples, which were sequenced and are shown in phylogenetic trees. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-04-04 |
dc.date.accessioned.fl_str_mv |
2019-01-03T11:42:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
STEFANELLO, Flávia. Quirópteros: população e potencial zoonótico em granjas de suínos. 2016. 69 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2016. |
dc.identifier.uri.fl_str_mv |
http://tede.upf.br/jspui/handle/tede/1595 |
identifier_str_mv |
STEFANELLO, Flávia. Quirópteros: população e potencial zoonótico em granjas de suínos. 2016. 69 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2016. |
url |
http://tede.upf.br/jspui/handle/tede/1595 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
794520062517004959 |
dc.relation.confidence.fl_str_mv |
500 500 600 |
dc.relation.department.fl_str_mv |
53202200503672799 |
dc.relation.cnpq.fl_str_mv |
453670264235017319 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Bioexperimentação |
dc.publisher.initials.fl_str_mv |
UPF |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia e Medicina Veterinária – FAMV |
publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.source.none.fl_str_mv |
reponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) instname:Universidade de Passo Fundo (UPF) instacron:UPF |
instname_str |
Universidade de Passo Fundo (UPF) |
instacron_str |
UPF |
institution |
UPF |
reponame_str |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
collection |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
bitstream.url.fl_str_mv |
http://tede.upf.br:8080/jspui/bitstream/tede/1595/1/license.txt http://tede.upf.br:8080/jspui/bitstream/tede/1595/2/2015FlaviaStefanello.pdf |
bitstream.checksum.fl_str_mv |
e0faded76e3df80302a4a0fb3f2bb5f3 93289aef7cf6bb8d5ce63b126801995b |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF) |
repository.mail.fl_str_mv |
biblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.br |
_version_ |
1809092292696342528 |