Produção de esporos e inoculação de Corynespora cassiicola em soja
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://10.0.217.128:8080/jspui/handle/tede/483 |
Resumo: | The target spot is found in most soybean producing areas of Brazil. The name of the disease is due to the concentric rings, with center and darker yellow halos present in the patches, which resemble the shape of a target. Premature defoliation may occur in susceptible cultivars, and the decay of many spots on pods and stems. The objectives of this study were to morphologically characterize the causal agent of an isolated target spot of soybean, quantify the sporulation of Corynespora cassiicola on different substrates; evaluate the effect of temperature on germination of conidia; assess the reaction of soybean cultivars to the pathogen and to quantify the effect of different concentrations of inoculum on the intensity of the disease. The experiments were conducted at the Laboratory of Plant, home-de-vegetation and climate chambers of the School of Agronomy and Veterinary Medicine, University of Passo Fundo - RS. Comparing the symptoms of the disease with the descriptions in literature and the reisolations of the pathogen, followed by their morphological characterization, it is confirmed the identification and pathogenicity of C. cassiicola isolated from leaves of soybean. Assessing the sporulation of C. cassiicola on different substrates (Potato Dextrose Agar, Czapek Solution Agar, infant food, Malt Agar, Oat Meal Agar and V8 juice), Czapek solution agar in the presence of light photoperiod 12 hours, plus filter paper showed greater sporulation of C. cassiicola. Among the tested temperatures (6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 36, 37, 38, 39, 40 and 41° C) to assess the germination of conidia of C. cassiicola, temperatures to 23° C was the lower thermal threshold was 7° C and higher than 39° C. The cultivar most susceptible among the tested cultivars (Apollo BMX, BMX Power, BRS 243 RR, BRS 255 RR, RR 214 CD, RR 219 CD, Fundacep 56 RR, 59 RR Fundacep, P98C11 Pioneer, Pioneer P98R31) the number of injuries by leaflet was BRX Power, and the diameter of lesion, BRX Apollo. Fundacep 56 and 59 were the most resistant cultivars to the pathogen, both in number and to diameter of lesion. It was demonstrated that the concentration of 35x10³ conídios.mL-¹ causes a level of disease that can be used in assessing the reaction of soybean cultivars |
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Reis, Erlei MeloCPF:00829897020http://lattes.cnpq.br/6225704270607677CPF:81369603053http://lattes.cnpq.br/6333885539763438Melo, Márcia Muliterno de2018-01-10T18:02:56Z2009-08-252009-04-13http://10.0.217.128:8080/jspui/handle/tede/483The target spot is found in most soybean producing areas of Brazil. The name of the disease is due to the concentric rings, with center and darker yellow halos present in the patches, which resemble the shape of a target. Premature defoliation may occur in susceptible cultivars, and the decay of many spots on pods and stems. The objectives of this study were to morphologically characterize the causal agent of an isolated target spot of soybean, quantify the sporulation of Corynespora cassiicola on different substrates; evaluate the effect of temperature on germination of conidia; assess the reaction of soybean cultivars to the pathogen and to quantify the effect of different concentrations of inoculum on the intensity of the disease. The experiments were conducted at the Laboratory of Plant, home-de-vegetation and climate chambers of the School of Agronomy and Veterinary Medicine, University of Passo Fundo - RS. Comparing the symptoms of the disease with the descriptions in literature and the reisolations of the pathogen, followed by their morphological characterization, it is confirmed the identification and pathogenicity of C. cassiicola isolated from leaves of soybean. Assessing the sporulation of C. cassiicola on different substrates (Potato Dextrose Agar, Czapek Solution Agar, infant food, Malt Agar, Oat Meal Agar and V8 juice), Czapek solution agar in the presence of light photoperiod 12 hours, plus filter paper showed greater sporulation of C. cassiicola. Among the tested temperatures (6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 36, 37, 38, 39, 40 and 41° C) to assess the germination of conidia of C. cassiicola, temperatures to 23° C was the lower thermal threshold was 7° C and higher than 39° C. The cultivar most susceptible among the tested cultivars (Apollo BMX, BMX Power, BRS 243 RR, BRS 255 RR, RR 214 CD, RR 219 CD, Fundacep 56 RR, 59 RR Fundacep, P98C11 Pioneer, Pioneer P98R31) the number of injuries by leaflet was BRX Power, and the diameter of lesion, BRX Apollo. Fundacep 56 and 59 were the most resistant cultivars to the pathogen, both in number and to diameter of lesion. It was demonstrated that the concentration of 35x10³ conídios.mL-¹ causes a level of disease that can be used in assessing the reaction of soybean cultivarsA mancha-alvo é encontrada na maioria das áreas produtoras de soja do Brasil. O nome da doença se deve aos anéis concêntricos, mais escuros no centro e halos amarelos presentes nas manchas, que lembram o formato de um alvo. Desfolha prematura pode ocorrer em cultivares suscetíveis, assim como o apodrecimento das vagens e numerosas manchas nas hastes. Justifica-se o trabalho considerando a ocorrência frequente da doença em lavouras da região centro oeste do país, assim como a falta de estudos relacionados a cultivares resistentes. Os objetivos desse estudo foram: caracterizar morfologicamente o agente causal de um isolado da mancha alvo da soja, quantificar a esporulação de Corynespora cassiicola em diferentes substratos; avaliar o efeito da temperatura na germinação de conídios; avaliar a reação de cultivares de soja ao patógeno e, quantificar o efeito de diferentes concentrações de inóculo sobre a intensidade da doença. Os experimentos foram conduzidos no Laboratório de Fitopatologia, em casa-de-vegetação e câmaras climatizadas da Faculdade de Agronomia e Medicina Veterinária da Universidade de Passo Fundo RS. Comparando os sintomas da doença com as descrições da literatura e com o reisolamento do patógeno, seguido de sua caracterização morfológica, confirmou-se à identificação e patogenicidade de C. cassiicola isolada de folhas de soja. Avaliando a esporulação de C. cassiicola em diferentes substratos (Batata Dextrose Ágar, Solução Czapek Ágar, Alimento infantil, Malte Ágar, Farinha de aveia e suco V8 Ágar), a Solução Czapek Ágar na presença de luz fotoperíodo 12 horas, acrescido de papel filtro apresentou maior esporulação de C. cassiicola. Entre as temperaturas testadas (6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 36, 37, 38, 39, 40 e 41°C) para avaliar a germinação de conídios de C. cassiicola, temperatura ótima para foi de 23°C, o limiar térmico inferior foi de 7°C e o superior de 39°C. O cultivar mais suscetível entre os cultivares testados (BMX Apolo, BMX Potência, BRS 243 RR, BRS 255 RR, CD 214 RR, CD 219 RR, Fundacep 56 RR, Fundacep 59 RR, Pioneer P98C11, Pioneer P98R31) ao número de lesão por folíolo foi BRX Potência, e ao diâmetro de lesão, BRX Apolo. Fundacep 56 e 59 foram os cultivares mais resistentes ao patógeno, tanto para número quanto para diâmetro de lesão. Demonstrou-se que a concentração de 35x10³ conídios.mL-1 causa uma intensidade da doença que pode ser usada na avaliação da reação de cultivares de sojaMade available in DSpace on 2018-01-10T18:02:56Z (GMT). No. of bitstreams: 1 2009MarciaMuliternodeMelo.pdf: 360511 bytes, checksum: fdf0d5efbdb656f342c8ee93b87c0bfc (MD5) Previous issue date: 2009-04-13application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em AgronomiaUPFBRFaculdade de Agronomia e Medicina Veterinária – FAMVSoja - CultivoFitopatologiaSoja - Doenças e pragasSoybeanPlant diseasesDiseases and pestsCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAProdução de esporos e inoculação de Corynespora cassiicola em sojaProduction of spores and inoculation of Corynespora cassiicola in soybeaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6356564600600600565656745226info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFORIGINAL2009MarciaMuliternodeMelo.pdfapplication/pdf360511http://tede.upf.br:8080/jspui/bitstream/tede/483/1/2009MarciaMuliternodeMelo.pdffdf0d5efbdb656f342c8ee93b87c0bfcMD51tede/4832018-09-06 15:46:20.593oai:tede.upf.br:tede/483Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2018-09-06T18:46:20Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
dc.title.alternative.eng.fl_str_mv |
Production of spores and inoculation of Corynespora cassiicola in soybean |
title |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
spellingShingle |
Produção de esporos e inoculação de Corynespora cassiicola em soja Melo, Márcia Muliterno de Soja - Cultivo Fitopatologia Soja - Doenças e pragas Soybean Plant diseases Diseases and pests CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
title_full |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
title_fullStr |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
title_full_unstemmed |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
title_sort |
Produção de esporos e inoculação de Corynespora cassiicola em soja |
author |
Melo, Márcia Muliterno de |
author_facet |
Melo, Márcia Muliterno de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Reis, Erlei Melo |
dc.contributor.advisor1ID.fl_str_mv |
CPF:00829897020 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6225704270607677 |
dc.contributor.authorID.fl_str_mv |
CPF:81369603053 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6333885539763438 |
dc.contributor.author.fl_str_mv |
Melo, Márcia Muliterno de |
contributor_str_mv |
Reis, Erlei Melo |
dc.subject.por.fl_str_mv |
Soja - Cultivo Fitopatologia Soja - Doenças e pragas |
topic |
Soja - Cultivo Fitopatologia Soja - Doenças e pragas Soybean Plant diseases Diseases and pests CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.eng.fl_str_mv |
Soybean Plant diseases Diseases and pests |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
description |
The target spot is found in most soybean producing areas of Brazil. The name of the disease is due to the concentric rings, with center and darker yellow halos present in the patches, which resemble the shape of a target. Premature defoliation may occur in susceptible cultivars, and the decay of many spots on pods and stems. The objectives of this study were to morphologically characterize the causal agent of an isolated target spot of soybean, quantify the sporulation of Corynespora cassiicola on different substrates; evaluate the effect of temperature on germination of conidia; assess the reaction of soybean cultivars to the pathogen and to quantify the effect of different concentrations of inoculum on the intensity of the disease. The experiments were conducted at the Laboratory of Plant, home-de-vegetation and climate chambers of the School of Agronomy and Veterinary Medicine, University of Passo Fundo - RS. Comparing the symptoms of the disease with the descriptions in literature and the reisolations of the pathogen, followed by their morphological characterization, it is confirmed the identification and pathogenicity of C. cassiicola isolated from leaves of soybean. Assessing the sporulation of C. cassiicola on different substrates (Potato Dextrose Agar, Czapek Solution Agar, infant food, Malt Agar, Oat Meal Agar and V8 juice), Czapek solution agar in the presence of light photoperiod 12 hours, plus filter paper showed greater sporulation of C. cassiicola. Among the tested temperatures (6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 36, 37, 38, 39, 40 and 41° C) to assess the germination of conidia of C. cassiicola, temperatures to 23° C was the lower thermal threshold was 7° C and higher than 39° C. The cultivar most susceptible among the tested cultivars (Apollo BMX, BMX Power, BRS 243 RR, BRS 255 RR, RR 214 CD, RR 219 CD, Fundacep 56 RR, 59 RR Fundacep, P98C11 Pioneer, Pioneer P98R31) the number of injuries by leaflet was BRX Power, and the diameter of lesion, BRX Apollo. Fundacep 56 and 59 were the most resistant cultivars to the pathogen, both in number and to diameter of lesion. It was demonstrated that the concentration of 35x10³ conídios.mL-¹ causes a level of disease that can be used in assessing the reaction of soybean cultivars |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-08-25 |
dc.date.issued.fl_str_mv |
2009-04-13 |
dc.date.accessioned.fl_str_mv |
2018-01-10T18:02:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
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http://10.0.217.128:8080/jspui/handle/tede/483 |
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http://10.0.217.128:8080/jspui/handle/tede/483 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6356564 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
565656 |
dc.relation.cnpq.fl_str_mv |
745226 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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Universidade de Passo Fundo |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Agronomia |
dc.publisher.initials.fl_str_mv |
UPF |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia e Medicina Veterinária – FAMV |
publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.source.none.fl_str_mv |
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Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
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biblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.br |
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