Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)

Detalhes bibliográficos
Autor(a) principal: Peruzzo, Sabrina Tolotti
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)
Texto Completo: http://tede.upf.br:8080/jspui/handle/tede/1931
Resumo: The high degree of genetic variability of plant populations, is one of the main features that allow the survival and adaptation of species under any environmental condition of stress caused to the environment. The continued use of a single herbicide or mechanism of action favors the selection of resistant weeds, which becomes serious problem in the areas of agricultural production. The weeds when living with the cultures interfere in their development and cause losses in productivity and quality of the final product grain. The Echium plantagineum L. species commonly known as flower - Purple was introduced in Brazil with an ornamental purpose, and it is considered a suitable species to produce honey. Currently its significance is related to weed plant, which occurs mainly in the south where inhabits grasslands and winter crops. Currently there are plants that are no longer controlled by the main herbicide used in areas, methyl metsulfurom, acetolactate synthase enzyme inhibitor (ALS). Aiming to confirm the resistance E. plantagineum the group of the ALS herbicides mechanism; assess whether the resistance is linked to the metabolism of the herbicide, as well as understanding the development of the species throughout its cycle and its characteristics morphoanatomic. Three experiments were conducted in greenhouse and laboratory during the years 2016 to 2018 at the University of Passo Fundo (UPF)/Passo Fundo. To evaluate the resistance of the biotypes experiment was conducted in a greenhouse in order to realize the dose response curve four ALS inhibitor herbicides (metsulfurom - methyl, bispyribac-sodium, and piroxulam imazethapyr). For the evaluation of enzymatic properties of ALS and resistance mechanisms unrelated to the site of action, the study-subdivided in two experiments. The first to assess the activity of the ALS enzyme in the presence of imazapic and the concentration of substrate (pyruvate) that provides initial speed equal to half the maximum reaction rate (KM) and maximum reaction velocity (Vmax). The second corresponded to the test metabolism by cytochrome P450 monooxygenase complex. Biotopes as evaluating the growth analysis, morphoanatomical chromosomes and counting were performed three experiments. From the studies it was unable to verify the resistance of the ALS enzyme biotypes, where they were cross-resistant. To assess the enzymatic activity it was found that there was a change of the kinetic parameters of the enzyme to the substrate which in turn also confers biotype resistance R and the high level of susceptibility biotypes S1 and S2. When assessing whether the resistance can be linked to metabolism study showed that there is involvement of the P450 complex in biotype resistance R. Finally parse that biotypes respond differently in the development being that sensitive biotypes have greater development of air already has tough Mays root system development capabilities. As the morphoanatomic features and chromosome counting not found to change.
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spelling Rizzardi, Mauro Antônio46984682053http://lattes.cnpq.br/296995787315093702032294010http://lattes.cnpq.br/7500709886570785Peruzzo, Sabrina Tolotti2021-02-11T14:52:59Z2019-10-25PERUZZO, Sabrina Tolotti. Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS). 2019. 99 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.http://tede.upf.br:8080/jspui/handle/tede/1931The high degree of genetic variability of plant populations, is one of the main features that allow the survival and adaptation of species under any environmental condition of stress caused to the environment. The continued use of a single herbicide or mechanism of action favors the selection of resistant weeds, which becomes serious problem in the areas of agricultural production. The weeds when living with the cultures interfere in their development and cause losses in productivity and quality of the final product grain. The Echium plantagineum L. species commonly known as flower - Purple was introduced in Brazil with an ornamental purpose, and it is considered a suitable species to produce honey. Currently its significance is related to weed plant, which occurs mainly in the south where inhabits grasslands and winter crops. Currently there are plants that are no longer controlled by the main herbicide used in areas, methyl metsulfurom, acetolactate synthase enzyme inhibitor (ALS). Aiming to confirm the resistance E. plantagineum the group of the ALS herbicides mechanism; assess whether the resistance is linked to the metabolism of the herbicide, as well as understanding the development of the species throughout its cycle and its characteristics morphoanatomic. Three experiments were conducted in greenhouse and laboratory during the years 2016 to 2018 at the University of Passo Fundo (UPF)/Passo Fundo. To evaluate the resistance of the biotypes experiment was conducted in a greenhouse in order to realize the dose response curve four ALS inhibitor herbicides (metsulfurom - methyl, bispyribac-sodium, and piroxulam imazethapyr). For the evaluation of enzymatic properties of ALS and resistance mechanisms unrelated to the site of action, the study-subdivided in two experiments. The first to assess the activity of the ALS enzyme in the presence of imazapic and the concentration of substrate (pyruvate) that provides initial speed equal to half the maximum reaction rate (KM) and maximum reaction velocity (Vmax). The second corresponded to the test metabolism by cytochrome P450 monooxygenase complex. Biotopes as evaluating the growth analysis, morphoanatomical chromosomes and counting were performed three experiments. From the studies it was unable to verify the resistance of the ALS enzyme biotypes, where they were cross-resistant. To assess the enzymatic activity it was found that there was a change of the kinetic parameters of the enzyme to the substrate which in turn also confers biotype resistance R and the high level of susceptibility biotypes S1 and S2. When assessing whether the resistance can be linked to metabolism study showed that there is involvement of the P450 complex in biotype resistance R. Finally parse that biotypes respond differently in the development being that sensitive biotypes have greater development of air already has tough Mays root system development capabilities. As the morphoanatomic features and chromosome counting not found to change.O elevado grau de variabilidade genética existente entre populações de plantas, é uma das principais características que permitem a sobrevivência e adaptação das espécies em qualquer condição ambiental de estresse provocado no ambiente. O uso contínuo de um único herbicida ou mecanismo de ação favorece a seleção de plantas daninhas resistentes, o que torna-se grave problema para as áreas de produção agrícola. As plantas daninhas quando convivendo junto com as culturas interferem no seu desenvolvimento e causam perdas na produtividade e na qualidade de grãos do produto final. A espécie Echium plantagineum L. popularmente conhecida como flor - roxa foi introduzida no Brasil com finalidade ornamental, e por ser considerada uma espécie apta a produção de mel. Atualmente sua significância está relacionada a planta infestante, que ocorre principalmente no Sul do país onde povoa campos nativos e culturas de inverno. Atualmente há plantas que não são mais controladas pelo principal herbicida utilizado nas áreas, o metsulfurom metílico, inibidor da enzima acetolactato sintase (ALS). Com o objetivo de confirmar a resistência de E. plantagineum ao grupo de herbicidas pertencentes ao mecanismo ALS; avaliar se a resistência está atrelada a metabolização do herbicida, além de compreender o desenvolvimento da espécie ao longo do seu ciclo e as suas características morfoanatômicas. Foram desenvolvidos três experimentos em casa de vegetação e laboratório durante os anos de 2016 a 2018 na Universidade de Passo Fundo (UPF)/ Passo Fundo. Para avaliação da resistência dos biótipos, conduziu-se experimento em casa de vegetação a fim de realizar a curva de dose resposta de quatro herbicidas inibidores da ALS (metsulfurom - metílico, bispiribaque-sodico, piroxulam e imazetapir). Para a avaliação das propriedades enzimáticas da ALS e mecanismos de resistência não relacionados ao local de ação, o estudo subdividiu-se em dois experimentos. O primeiro para avaliar a atividade da enzima ALS na presença do herbicida imazapic e a concentração de substrato (piruvato) que fornece velocidade inicial igual à metade da velocidade máxima de reação (KM) e a velocidade máxima de reação (Vmáx). Já o segundo correspondeu ao experimento de metabolização pelo complexo citocromo P450 monooxigenase. Avaliando os biótipos quanto a análise de crescimento, caracterização morfoanatômica e contagem de cromossomos, foram realizados três experimentos. A partir dos estudos foi possível confirmar a resistência dos biótipos a enzima ALS, onde os mesmos apresentaram resistência cruzada. Para a avaliação da atividade enzimática constatou-se que houve alteração dos parâmetros cinéticos da enzima em relação ao substrato o que por sua vez também confere resistência do biótipo R e alto nível de suscetibilidade dos biótipos S1 e S2. Quando avaliando se a resistência pode estar atrelada a metabolização o estudo mostrou que há envolvimento do complexo P450 na resistência do biótipo R. E por fim foi possível analisar que biótipos respondem de maneira distinta quanto ao desenvolvimento sendo que os biótipos sensíveis apresentam maior desenvolvimento de parte aérea já o resistente tem maios capacidade de desenvolvimento de sistema radicular. Quanto as características morfoanatômicas e contagem cromossômica não verificou-se alterações.Submitted by Aline Rezende (alinerezende@upf.br) on 2021-02-11T14:52:59Z No. of bitstreams: 1 2019SabrinaTolottiPeruzzo.pdf: 1559195 bytes, checksum: ccc6dea1443ff1344075646e13511f27 (MD5)Made available in DSpace on 2021-02-11T14:52:59Z (GMT). No. of bitstreams: 1 2019SabrinaTolottiPeruzzo.pdf: 1559195 bytes, checksum: ccc6dea1443ff1344075646e13511f27 (MD5) Previous issue date: 2019-10-25application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em AgronomiaUPFBrasilFaculdade de Agronomia e Medicina Veterinária – FAMVFlores - CultivoPlantas - Efeito dos herbícidasInibidores enzimáticosCIENCIAS AGRARIAS::AGRONOMIACaracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)Characterization of the resistance of Echium plantagineum to the inhibiting herbicides of the enzyme acetolactate synthase (ALS)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis562406611703505429050050060053202200503672799-3091138714907603907info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFORIGINAL2019SabrinaTolottiPeruzzo.pdf2019SabrinaTolottiPeruzzo.pdfapplication/pdf1559195http://tede.upf.br:8080/jspui/bitstream/tede/1931/2/2019SabrinaTolottiPeruzzo.pdfccc6dea1443ff1344075646e13511f27MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.upf.br:8080/jspui/bitstream/tede/1931/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/19312022-01-25 12:26:32.319oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2022-01-25T14:26:32Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false
dc.title.por.fl_str_mv Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
dc.title.alternative.eng.fl_str_mv Characterization of the resistance of Echium plantagineum to the inhibiting herbicides of the enzyme acetolactate synthase (ALS)
title Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
spellingShingle Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
Peruzzo, Sabrina Tolotti
Flores - Cultivo
Plantas - Efeito dos herbícidas
Inibidores enzimáticos
CIENCIAS AGRARIAS::AGRONOMIA
title_short Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
title_full Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
title_fullStr Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
title_full_unstemmed Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
title_sort Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS)
author Peruzzo, Sabrina Tolotti
author_facet Peruzzo, Sabrina Tolotti
author_role author
dc.contributor.advisor1.fl_str_mv Rizzardi, Mauro Antônio
dc.contributor.advisor1ID.fl_str_mv 46984682053
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2969957873150937
dc.contributor.authorID.fl_str_mv 02032294010
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7500709886570785
dc.contributor.author.fl_str_mv Peruzzo, Sabrina Tolotti
contributor_str_mv Rizzardi, Mauro Antônio
dc.subject.por.fl_str_mv Flores - Cultivo
Plantas - Efeito dos herbícidas
Inibidores enzimáticos
topic Flores - Cultivo
Plantas - Efeito dos herbícidas
Inibidores enzimáticos
CIENCIAS AGRARIAS::AGRONOMIA
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::AGRONOMIA
description The high degree of genetic variability of plant populations, is one of the main features that allow the survival and adaptation of species under any environmental condition of stress caused to the environment. The continued use of a single herbicide or mechanism of action favors the selection of resistant weeds, which becomes serious problem in the areas of agricultural production. The weeds when living with the cultures interfere in their development and cause losses in productivity and quality of the final product grain. The Echium plantagineum L. species commonly known as flower - Purple was introduced in Brazil with an ornamental purpose, and it is considered a suitable species to produce honey. Currently its significance is related to weed plant, which occurs mainly in the south where inhabits grasslands and winter crops. Currently there are plants that are no longer controlled by the main herbicide used in areas, methyl metsulfurom, acetolactate synthase enzyme inhibitor (ALS). Aiming to confirm the resistance E. plantagineum the group of the ALS herbicides mechanism; assess whether the resistance is linked to the metabolism of the herbicide, as well as understanding the development of the species throughout its cycle and its characteristics morphoanatomic. Three experiments were conducted in greenhouse and laboratory during the years 2016 to 2018 at the University of Passo Fundo (UPF)/Passo Fundo. To evaluate the resistance of the biotypes experiment was conducted in a greenhouse in order to realize the dose response curve four ALS inhibitor herbicides (metsulfurom - methyl, bispyribac-sodium, and piroxulam imazethapyr). For the evaluation of enzymatic properties of ALS and resistance mechanisms unrelated to the site of action, the study-subdivided in two experiments. The first to assess the activity of the ALS enzyme in the presence of imazapic and the concentration of substrate (pyruvate) that provides initial speed equal to half the maximum reaction rate (KM) and maximum reaction velocity (Vmax). The second corresponded to the test metabolism by cytochrome P450 monooxygenase complex. Biotopes as evaluating the growth analysis, morphoanatomical chromosomes and counting were performed three experiments. From the studies it was unable to verify the resistance of the ALS enzyme biotypes, where they were cross-resistant. To assess the enzymatic activity it was found that there was a change of the kinetic parameters of the enzyme to the substrate which in turn also confers biotype resistance R and the high level of susceptibility biotypes S1 and S2. When assessing whether the resistance can be linked to metabolism study showed that there is involvement of the P450 complex in biotype resistance R. Finally parse that biotypes respond differently in the development being that sensitive biotypes have greater development of air already has tough Mays root system development capabilities. As the morphoanatomic features and chromosome counting not found to change.
publishDate 2019
dc.date.issued.fl_str_mv 2019-10-25
dc.date.accessioned.fl_str_mv 2021-02-11T14:52:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv PERUZZO, Sabrina Tolotti. Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS). 2019. 99 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.
dc.identifier.uri.fl_str_mv http://tede.upf.br:8080/jspui/handle/tede/1931
identifier_str_mv PERUZZO, Sabrina Tolotti. Caracterização da resistência de Echium plantagineum aos herbicidas inibidores da enzima acetolactato sintase (ALS). 2019. 99 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.
url http://tede.upf.br:8080/jspui/handle/tede/1931
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language por
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dc.publisher.initials.fl_str_mv UPF
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Faculdade de Agronomia e Medicina Veterinária – FAMV
publisher.none.fl_str_mv Universidade de Passo Fundo
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