Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil

Detalhes bibliográficos
Autor(a) principal: GUERRA, Neurisvan Ramos
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5059
Resumo: Trypanosoma vivax determines losses in production of ruminants related to morbidity, decline in production, reproductive problems and also mortality. The objective of this study was to determine the diagnosis of Trypanosoma (Dutonella) vivax in cattle of different regions of Pernambuco State, Brazil, through the techniques of Indirect Immunofluorescence Assay (IFA) and Polymerase Chain Reaction (PCR). Firstly, it was performed a serology of 2,053 serum samples from bovine herds from different counties in the state of Pernambuco. From these results the counties with the highest frequency by IFA test were selected and a total of 127 bovine blood samples were collected for molecular analysis by PCR. The results of IFAT showed 13.93% (286/2,053) of positive animals for IgG antibodies against Trypanosoma vivax. The amplification by PCR revealed positivity of 44.88% (57/127). These results suggest that Pernambuco state is an area endemic for Trypanosoma vivax being considered an enzootic instability area. On the other hand, PCR was effective in the diagnosis of Trypanosoma vivax in cattle blood, being an indispensable tool for studies epidemiological.
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spelling ALVES, Leucio CâmaraSANTANA, Vânia Lucia de AssisFARIAS, Márcia Paula OliveiraMAIA, Carine Scanonihttp://lattes.cnpq.br/4215328809716471GUERRA, Neurisvan Ramos2016-07-25T13:02:29Z2013-02-20GUERRA, Neurisvan Ramos. Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil. 2013.46 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5059Trypanosoma vivax determines losses in production of ruminants related to morbidity, decline in production, reproductive problems and also mortality. The objective of this study was to determine the diagnosis of Trypanosoma (Dutonella) vivax in cattle of different regions of Pernambuco State, Brazil, through the techniques of Indirect Immunofluorescence Assay (IFA) and Polymerase Chain Reaction (PCR). Firstly, it was performed a serology of 2,053 serum samples from bovine herds from different counties in the state of Pernambuco. From these results the counties with the highest frequency by IFA test were selected and a total of 127 bovine blood samples were collected for molecular analysis by PCR. The results of IFAT showed 13.93% (286/2,053) of positive animals for IgG antibodies against Trypanosoma vivax. The amplification by PCR revealed positivity of 44.88% (57/127). These results suggest that Pernambuco state is an area endemic for Trypanosoma vivax being considered an enzootic instability area. On the other hand, PCR was effective in the diagnosis of Trypanosoma vivax in cattle blood, being an indispensable tool for studies epidemiological.Trypanosoma vivax determina prejuízos à produção de ruminantes, relacionados com a morbidade, queda na produção, problemas reprodutivos além de mortalidade. O presente trabalho teve como objetivo realizar o diagnóstico de Trypanosoma (Dutonella) vivax em bovinos das diferentes Mesorregiões do Estado de Pernambuco, Brasil, através das técnicas de Reação de Imunofluorescência Indireta (RIFI) e da Reação em Cadeia da Polimerase (PCR). Iniciamente foi realizada sorologia de 2.053 amostras de soro sanguíneo de bovinos provenientes de rebanhos de municípios do estado de Pernambuco. A partir destes resultados foram selecionados os municípios com maiores frequências de anticorpos em cada mesorregião do Estado de Pernambuco e foram coletadas um total de 127 amostras de sangue bovino para análise molecular através da PCR. À sorologia, 13,93% (286/2.053) dos animais foram reagentes para anticorpos IgG anti-Trypanosoma vivax. Já na PCR foi obtida uma positividade de 44,88% (57/127). Esses resultados sugerem que o estado de Pernambuco é área endêmica para Trypanosoma vivax, apresentando-se como área de instabilidade enzoótica e que a PCR mostrou-se eficiente no diagnóstico da infecção por Trypanosoma vivax em sangue de bovinos, sendo uma ferramenta indispensável para estudos epidemiológicos.Submitted by (edna.saturno@ufrpe.br) on 2016-07-25T13:02:29Z No. of bitstreams: 1 Neurisvan Ramos Guerra.pdf: 506781 bytes, checksum: 5f3717d4e83a80c6435609c31fc492be (MD5)Made available in DSpace on 2016-07-25T13:02:29Z (GMT). No. of bitstreams: 1 Neurisvan Ramos Guerra.pdf: 506781 bytes, checksum: 5f3717d4e83a80c6435609c31fc492be (MD5) Previous issue date: 2013-02-20application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência Animal TropicalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalTripanossomíaseDiagnósticoAnticorposImunofluorescência indiretaReação em cadeia da polimeraseDNATripanossomiasisAntibodiesIndirect imunofluorescencePolymerase chain reactionCIENCIAS AGRARIAS::MEDICINA VETERINARIATrypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-6143187600765506511600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5059/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALNeurisvan Ramos Guerra.pdfNeurisvan Ramos Guerra.pdfapplication/pdf506781http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5059/2/Neurisvan+Ramos+Guerra.pdf5f3717d4e83a80c6435609c31fc492beMD52tede2/50592017-02-10 12:10:47.113oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:32:32.494871Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
title Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
spellingShingle Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
GUERRA, Neurisvan Ramos
Tripanossomíase
Diagnóstico
Anticorpos
Imunofluorescência indireta
Reação em cadeia da polimerase
DNA
Tripanossomiasis
Antibodies
Indirect imunofluorescence
Polymerase chain reaction
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
title_full Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
title_fullStr Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
title_full_unstemmed Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
title_sort Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil
author GUERRA, Neurisvan Ramos
author_facet GUERRA, Neurisvan Ramos
author_role author
dc.contributor.advisor1.fl_str_mv ALVES, Leucio Câmara
dc.contributor.referee1.fl_str_mv SANTANA, Vânia Lucia de Assis
dc.contributor.referee2.fl_str_mv FARIAS, Márcia Paula Oliveira
dc.contributor.referee3.fl_str_mv MAIA, Carine Scanoni
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4215328809716471
dc.contributor.author.fl_str_mv GUERRA, Neurisvan Ramos
contributor_str_mv ALVES, Leucio Câmara
SANTANA, Vânia Lucia de Assis
FARIAS, Márcia Paula Oliveira
MAIA, Carine Scanoni
dc.subject.por.fl_str_mv Tripanossomíase
Diagnóstico
Anticorpos
Imunofluorescência indireta
Reação em cadeia da polimerase
DNA
topic Tripanossomíase
Diagnóstico
Anticorpos
Imunofluorescência indireta
Reação em cadeia da polimerase
DNA
Tripanossomiasis
Antibodies
Indirect imunofluorescence
Polymerase chain reaction
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv Tripanossomiasis
Antibodies
Indirect imunofluorescence
Polymerase chain reaction
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description Trypanosoma vivax determines losses in production of ruminants related to morbidity, decline in production, reproductive problems and also mortality. The objective of this study was to determine the diagnosis of Trypanosoma (Dutonella) vivax in cattle of different regions of Pernambuco State, Brazil, through the techniques of Indirect Immunofluorescence Assay (IFA) and Polymerase Chain Reaction (PCR). Firstly, it was performed a serology of 2,053 serum samples from bovine herds from different counties in the state of Pernambuco. From these results the counties with the highest frequency by IFA test were selected and a total of 127 bovine blood samples were collected for molecular analysis by PCR. The results of IFAT showed 13.93% (286/2,053) of positive animals for IgG antibodies against Trypanosoma vivax. The amplification by PCR revealed positivity of 44.88% (57/127). These results suggest that Pernambuco state is an area endemic for Trypanosoma vivax being considered an enzootic instability area. On the other hand, PCR was effective in the diagnosis of Trypanosoma vivax in cattle blood, being an indispensable tool for studies epidemiological.
publishDate 2013
dc.date.issued.fl_str_mv 2013-02-20
dc.date.accessioned.fl_str_mv 2016-07-25T13:02:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUERRA, Neurisvan Ramos. Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil. 2013.46 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5059
identifier_str_mv GUERRA, Neurisvan Ramos. Trypanosoma (Dutonella) vivax (Ziemann, 1905) em bovinos das diferentes mesorregiões do Estado de Pernambuco, Brasil. 2013.46 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5059
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language por
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600
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dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência Animal Tropical
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Morfologia e Fisiologia Animal
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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