Influência da melatonina exógena sobre parâmetros sanguíneos, hormonais, metabólicos e inflamatórios em ratas alimentadas com dieta hipercalórica durante a prenhez

• Autor(a) principal: SILVA, Solange Bezerra da
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
• Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4475
• topic: Parâmetro metabólico; Melatonina; Obesidade; Melatonin; Obesity; Metabolic parameter; CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Nowadays researchers have tried treatment strategies such as melatonin to slow down the symptoms of comorbidities related to obesity, since it has been associated with reduced body weight. Thus, the present study aimed to evaluate metabolic, biochemical and immunohistochemical parameters in the liver, as well as analyzing the interaction of melatonin with renal histophysiology and the immune system in pregnant rats fed with high calorie diet with or without this hormone.We used 40 pregnant rats divided in the groups (n=10): I: rats subjected to the common diet (D); II: rats submitted to high calorie diet (DH); III: rats submitted to high calorie diet + melatonin (0.5 mg / kg) (DH + Mel) and IV: rats subjected to the common diet + melatonin (0.5 mg / kg) (D + Mel). The results showed that at day 7 females of DH and DH + Mel groups showed body weight gain. At 14th and 20st day, only the females from DH group retained this condition. There were no differences for the solid and liquid intake, in the 7th day. At 14th and 20st day, there was an increase of this parameter in females of DH group. The serum levels of ALT, AST, Gamma GT, alkaline phosphatase, amylase, lipase, fructosamine, total cholesterol, LDL, triglycerides, HDL, glucose and insulin increased at 7th and 14th day in females of DH group, with the exception of amylase levels that did not differ between females of the groups (day 7) and reduced HDL and AF (day 14). In females of the groups treated with melatonin, amylase levels were reduced. At 20st day, the females of DH group showed the same behavior observed at 14 days. However, females who received melatonin showed increased FA and HDL, while the other parameters showed reduced values on the D and DH groups. The amount of lipids in the liver as well as the collagen content was significantly higher in females of DH group compared to other groups. In females of DH + Mel group there was a significant decrease in the amount of fat and collagen. In females of groups D, DH + Meland D + Mel there were no differences in relation to the amount of hepatic glycogen, differing from the females of DH group that had elevated levels relative to these.It was observed that females from DH group had hematological, urine and biochemical significant changes, as the improve levels of urea, creatinine, glucose, bilirubin, leukocytes, and platelets, and these levels normalized by administration of melatonin. Furthermore, the marking of collagen and proinflammatory cytokines in spleen (IL-6 and TNF-α) was elevated and reduced VEGF. Exogenous melatonin was shown to be an effective alternative therapy, with various anti-obesity effects in rats during pregnancy and reducing the adverse effects caused by obesity.