Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
dARK ID: | ark:/57462/0013000009sw5 |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8776 |
Resumo: | Medicinal plants and their secondary metabolites represent an important source for the development of new, more effective, and safer therapeutic options for pain management, including pain associated with cancer. In this sense, the central objective of this study was to evaluate the antinociceptive activity of the essential oil of Annona vepretorum (Av-OE) and of the (E)-β-ocimene free and complexed in β-cyclodextrin (β-CD) in an experimental model of cancer pain. The first chapter of the work provides an overview of the chemistry of monoterpenes and drug delivery systems applied to monoterpenes with analgesic potential. The second chapter provides evidence that oral treatment with Av-OE (50 and 100 mg/kg) was able to attenuate the process of hyperalgesia and/or allodynia induced by the presence of tumor in the mice. In addition, it was observed that the animals treated with the dose of 100 mg/kg showed a reduction in edema/paw tumor on the 12th day of observation (0.37 ± 0.02 mL). The last chapter initially describes the process of obtaining and physicochemical characterization of the ocimene inclusion complex in β-CD (ocimene/β-CD), whose results suggested the adequate complexation. It was also demonstrated that there was a decrease in the cytotoxicity of ocimene/β-CD against tumor cell lines (CI50 > 5 μg/mL), non-tumor cell line (CI50 > 5 μg/mL) and Artemia salina larvae (CL50 > 1000 μg/mL) when compared to free ocimene. In contrast, ocimene/β-CD showed greater hemolytic potential in the presence of erythrocytes from Swiss mice (CH50 = 134.3 ± 3.61 μg/mL). A lethal dose 50% (LD50) value greater than 300 mg/kg was estimated when assessing the acute in vivo toxicity of free and complexed ocimene. The administration of free ocimene or ocimene/β-CD (25 and 50 mg/kg) orally in mice with a solid tumor of sarcoma 180 in the right hind paw was able to reduce cancer pain and tumor volume, with possible participation in the modulation of the inflammatory response, since there was a significant increase in the levels of the anti-inflammatory cytokine IL-10 in the serum of animals treated with ocimene (15.98 ± 4.04 pg/mL) when compared with the sham group (6.73 ± 0.43 pg/mL). The evaluation of hematological parameters suggested less toxicity of the ocimene when complexed. Thus, treatment with the essential oil of A. vepretorum or with the inclusion complex containing (E)-β-ocimene and β-CD has antinociceptive activity in mice with solid sarcoma tumor 180. |
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ALMEIDA, Jackson Roberto Guedes da SilvaPALHETA JUNIOR, Raimundo CamposMENEZES, Irwin Rose Alencar deQUINTANS JÚNIOR, Lucindo JoséGUIMARÃES, Adriana Gibarahttp://lattes.cnpq.br/2755665501425287SILVA, Mariana Gama e2022-12-22T16:09:48Z2021-02-26SILVA, Mariana Gama e. Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica. 2021. 134 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife .http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8776ark:/57462/0013000009sw5Medicinal plants and their secondary metabolites represent an important source for the development of new, more effective, and safer therapeutic options for pain management, including pain associated with cancer. In this sense, the central objective of this study was to evaluate the antinociceptive activity of the essential oil of Annona vepretorum (Av-OE) and of the (E)-β-ocimene free and complexed in β-cyclodextrin (β-CD) in an experimental model of cancer pain. The first chapter of the work provides an overview of the chemistry of monoterpenes and drug delivery systems applied to monoterpenes with analgesic potential. The second chapter provides evidence that oral treatment with Av-OE (50 and 100 mg/kg) was able to attenuate the process of hyperalgesia and/or allodynia induced by the presence of tumor in the mice. In addition, it was observed that the animals treated with the dose of 100 mg/kg showed a reduction in edema/paw tumor on the 12th day of observation (0.37 ± 0.02 mL). The last chapter initially describes the process of obtaining and physicochemical characterization of the ocimene inclusion complex in β-CD (ocimene/β-CD), whose results suggested the adequate complexation. It was also demonstrated that there was a decrease in the cytotoxicity of ocimene/β-CD against tumor cell lines (CI50 > 5 μg/mL), non-tumor cell line (CI50 > 5 μg/mL) and Artemia salina larvae (CL50 > 1000 μg/mL) when compared to free ocimene. In contrast, ocimene/β-CD showed greater hemolytic potential in the presence of erythrocytes from Swiss mice (CH50 = 134.3 ± 3.61 μg/mL). A lethal dose 50% (LD50) value greater than 300 mg/kg was estimated when assessing the acute in vivo toxicity of free and complexed ocimene. The administration of free ocimene or ocimene/β-CD (25 and 50 mg/kg) orally in mice with a solid tumor of sarcoma 180 in the right hind paw was able to reduce cancer pain and tumor volume, with possible participation in the modulation of the inflammatory response, since there was a significant increase in the levels of the anti-inflammatory cytokine IL-10 in the serum of animals treated with ocimene (15.98 ± 4.04 pg/mL) when compared with the sham group (6.73 ± 0.43 pg/mL). The evaluation of hematological parameters suggested less toxicity of the ocimene when complexed. Thus, treatment with the essential oil of A. vepretorum or with the inclusion complex containing (E)-β-ocimene and β-CD has antinociceptive activity in mice with solid sarcoma tumor 180.As plantas medicinais e os seus metabólitos secundários representam uma importante fonte para o desenvolvimento de novas opções terapêuticas mais eficazes e seguras para o manejo da dor crônica, incluindo a dor associada ao câncer. Nesse sentido, o objetivo central desse estudo foi avaliar a atividade antinociceptiva do óleo essencial de Annona vepretorum (Av-OE) e do (E)-β-ocimeno complexado e não complexado em β-ciclodextrina (β-CD) em modelo experimental de dor oncológica. O primeiro capítulo do trabalho traz uma visão geral sobre a química dos monoterpenos e os sistemas de liberação de fármacos aplicados a monoterpenos com potencial analgésico. O segundo capítulo fornece evidências de que o tratamento por via oral com Av-OE (50 e 100 mg/kg) foi capaz de atenuar o processo de hiperalgesia e/ou alodinia induzidos pela presença do tumor nos camundongos. Além disso, observou-se que os animais tratados com a dose de 100 mg/kg apresentaram diminuição do edema/tumor de pata no 12º dia de observação (0,37 ± 0,02 mL). O último capítulo descreve inicialmente o processo de obtenção e caracterização físico-química do complexo de inclusão do (E)-β-ocimeno em β-CD (ocimeno/β-CD), cujos resultados sugeriram a adequada complexação. A triagem de toxicidade demonstrou que houve uma diminuição da citotoxicidade do ocimeno/β-CD diante de linhagens de células tumorais (CI50 > 5 μg/mL), linhagem celular não tumoral (CI50 > 5 μg/mL) e de larvas de Artemia salina (CL50 > 1000 μg/mL) se comparado ao ocimeno não complexado. Contrariamente, ocimeno/β-CD mostrou maior potencial hemolítico diante de eritrócitos de camundongos Swiss (CH50 = 134,3 ± 3,61 μg/mL). Estimou-se um valor de dose letal 50% (DL50) superior a 300 mg/kg quando avaliada a toxicidade aguda in vivo do ocimeno livre e complexado. A administração de ocimeno não complexado ou ocimeno/β-CD (25 e 50 mg/kg) por via oral em camundongos portadores de tumor sólido do sarcoma 180 na pata direita traseira foi capaz de reduzir a dor oncológica e o volume tumoral, com possível participação na modulação da resposta inflamatória, já que houve aumento significativo dos níveis da citocina anti-inflamatória IL-10 no soro dos animais tratados com ocimeno (15,98 ± 4,04 pg/mL) quando comparado com o grupo sham (6,73 ± 0,43 pg/mL). A avaliação dos parâmetros hematológicos sugeriu menor toxicidade do ocimeno quando complexado. Dessa forma, o tratamento com o óleo essencial de A. vepretorum ou com o complexo de inclusão contendo (E)-β-ocimeno e β-CD apresenta atividade antinociceptiva em camundongos portadores de tumor sólido de sarcoma 180.Submitted by (lucia.rodrigues@ufrpe.br) on 2022-12-22T16:09:48Z No. of bitstreams: 1 Mariana Gama e Silva.pdf: 2662751 bytes, checksum: 5bc28da1910123f8fc6a662313d1bcf3 (MD5)Made available in DSpace on 2022-12-22T16:09:48Z (GMT). No. of bitstreams: 1 Mariana Gama e Silva.pdf: 2662751 bytes, checksum: 5bc28da1910123f8fc6a662313d1bcf3 (MD5) Previous issue date: 2021-02-26Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biotecnologia (Renorbio)UFRPEBrasilRede Nordeste de BiotecnologiaCâncerProduto naturalComposto orgânicoHidrocarbonetoBiotecnologiaÓleo essencialAnnona vepretorumPlanta medicinalOUTROS::CIENCIASAvaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis7794227690756777355600600600600-810457658845227642162099577914943238252075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALMariana Gama e Silva.pdfMariana Gama e Silva.pdfapplication/pdf2662751http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8776/2/Mariana+Gama+e+Silva.pdf5bc28da1910123f8fc6a662313d1bcf3MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8776/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/87762022-12-22 13:09:48.367oai:tede2:tede2/8776Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:37:25.033754Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
title |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
spellingShingle |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica SILVA, Mariana Gama e Câncer Produto natural Composto orgânico Hidrocarboneto Biotecnologia Óleo essencial Annona vepretorum Planta medicinal OUTROS::CIENCIAS |
title_short |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
title_full |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
title_fullStr |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
title_full_unstemmed |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
title_sort |
Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica |
author |
SILVA, Mariana Gama e |
author_facet |
SILVA, Mariana Gama e |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
ALMEIDA, Jackson Roberto Guedes da Silva |
dc.contributor.referee1.fl_str_mv |
PALHETA JUNIOR, Raimundo Campos |
dc.contributor.referee2.fl_str_mv |
MENEZES, Irwin Rose Alencar de |
dc.contributor.referee3.fl_str_mv |
QUINTANS JÚNIOR, Lucindo José |
dc.contributor.referee4.fl_str_mv |
GUIMARÃES, Adriana Gibara |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2755665501425287 |
dc.contributor.author.fl_str_mv |
SILVA, Mariana Gama e |
contributor_str_mv |
ALMEIDA, Jackson Roberto Guedes da Silva PALHETA JUNIOR, Raimundo Campos MENEZES, Irwin Rose Alencar de QUINTANS JÚNIOR, Lucindo José GUIMARÃES, Adriana Gibara |
dc.subject.por.fl_str_mv |
Câncer Produto natural Composto orgânico Hidrocarboneto Biotecnologia Óleo essencial Annona vepretorum Planta medicinal |
topic |
Câncer Produto natural Composto orgânico Hidrocarboneto Biotecnologia Óleo essencial Annona vepretorum Planta medicinal OUTROS::CIENCIAS |
dc.subject.cnpq.fl_str_mv |
OUTROS::CIENCIAS |
description |
Medicinal plants and their secondary metabolites represent an important source for the development of new, more effective, and safer therapeutic options for pain management, including pain associated with cancer. In this sense, the central objective of this study was to evaluate the antinociceptive activity of the essential oil of Annona vepretorum (Av-OE) and of the (E)-β-ocimene free and complexed in β-cyclodextrin (β-CD) in an experimental model of cancer pain. The first chapter of the work provides an overview of the chemistry of monoterpenes and drug delivery systems applied to monoterpenes with analgesic potential. The second chapter provides evidence that oral treatment with Av-OE (50 and 100 mg/kg) was able to attenuate the process of hyperalgesia and/or allodynia induced by the presence of tumor in the mice. In addition, it was observed that the animals treated with the dose of 100 mg/kg showed a reduction in edema/paw tumor on the 12th day of observation (0.37 ± 0.02 mL). The last chapter initially describes the process of obtaining and physicochemical characterization of the ocimene inclusion complex in β-CD (ocimene/β-CD), whose results suggested the adequate complexation. It was also demonstrated that there was a decrease in the cytotoxicity of ocimene/β-CD against tumor cell lines (CI50 > 5 μg/mL), non-tumor cell line (CI50 > 5 μg/mL) and Artemia salina larvae (CL50 > 1000 μg/mL) when compared to free ocimene. In contrast, ocimene/β-CD showed greater hemolytic potential in the presence of erythrocytes from Swiss mice (CH50 = 134.3 ± 3.61 μg/mL). A lethal dose 50% (LD50) value greater than 300 mg/kg was estimated when assessing the acute in vivo toxicity of free and complexed ocimene. The administration of free ocimene or ocimene/β-CD (25 and 50 mg/kg) orally in mice with a solid tumor of sarcoma 180 in the right hind paw was able to reduce cancer pain and tumor volume, with possible participation in the modulation of the inflammatory response, since there was a significant increase in the levels of the anti-inflammatory cytokine IL-10 in the serum of animals treated with ocimene (15.98 ± 4.04 pg/mL) when compared with the sham group (6.73 ± 0.43 pg/mL). The evaluation of hematological parameters suggested less toxicity of the ocimene when complexed. Thus, treatment with the essential oil of A. vepretorum or with the inclusion complex containing (E)-β-ocimene and β-CD has antinociceptive activity in mice with solid sarcoma tumor 180. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-02-26 |
dc.date.accessioned.fl_str_mv |
2022-12-22T16:09:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Mariana Gama e. Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica. 2021. 134 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife . |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8776 |
dc.identifier.dark.fl_str_mv |
ark:/57462/0013000009sw5 |
identifier_str_mv |
SILVA, Mariana Gama e. Avaliação da atividade antinociceptiva do óleo essencial de Annona vepretorum Mart. (Annonaceae) e do complexo de inclusão do ocimeno com β-ciclodextrina em modelo experimental de dor oncológica. 2021. 134 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife . ark:/57462/0013000009sw5 |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8776 |
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por |
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por |
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Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biotecnologia (Renorbio) |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Rede Nordeste de Biotecnologia |
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Universidade Federal Rural de Pernambuco |
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institution |
UFRPE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
collection |
Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8776/2/Mariana+Gama+e+Silva.pdf http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8776/1/license.txt |
bitstream.checksum.fl_str_mv |
5bc28da1910123f8fc6a662313d1bcf3 bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
_version_ |
1818606201988972544 |