Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8191 |
Resumo: | Heavy metals are elements that are potentially toxic to natural ecosystems and to human health, and may originate from natural or anthropogenic sources. In Brazil an important case of environmental contamination involving heavy metals occurred in Santo Amaro, Bahia. One foundry company left an environmental liability of approximately 500,000 tonnes of metals containing mainly cadmium (Cd), lead (Pb) and zinc (Zn). Soils near the slag contain heavy metals in concentrations above permissible limits, even after years of interruption of metallurgical activity. In this sense, remediation techniques are needed to minimize the effects of metals on contaminated soils and restore ecosystem functionality around the factory. The present work has the objective of evaluating the potential of two legume species (mucuna and bean), inoculated with rhizobia, for use in phytoremediation of contaminated soils. In addition, the occurrence and morphological characteristics of the naturally established rhizobia populations in contaminated soils were evaluated using the two legume species cultivated without inoculation as bait plants. The legumes were cultivated in pots containing soil samples collected at points with increasing distances from the source of contamination, to represent the effect of different concentrations of the metals in the soil, and inoculated with nitrogen - fixing bacteria. Soils presented values of Pb and Cd above the value of investigation, signaling the need for remediation. The plants used presented symptoms of toxicity induced by the excess of heavy metals, however being tolerant, evaluated as a function of biomass production. The populations of native rhizobia of contaminated soils are tolerant and resistant to high concentrations of metals, such as: Cd, Pb and Zn. However, the high concentrations of heavy metals affected the nodulation and consequently the amount of N fixed. The phenotypic characteristics of these isolates showed a very high diversity, unusual, given the amount of isolates. Therefore, further studies with molecular tools to determine which species are able to nodulate in an environment as inhospitable as soils contaminated with heavy metals. |
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FREITAS, Ana Dolores Santiago deNASCIMENTO, Clístenes Williams Araújo doFREITAS, Ana Dolores Santiago deBIONDI, Caroline MirandaSILVA, Vinícius Santos Gomes dahttp://lattes.cnpq.br/5807881476724702SILVA, Jessyca Adriana Gomes Florêncio da2019-09-13T13:24:41Z2019-02-22SILVA, Jessyca Adriana Gomes Florêncio da. Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias. 2019. 68 f. Dissertação (Programa de Pós-Graduação em Ciência do Solo) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8191Heavy metals are elements that are potentially toxic to natural ecosystems and to human health, and may originate from natural or anthropogenic sources. In Brazil an important case of environmental contamination involving heavy metals occurred in Santo Amaro, Bahia. One foundry company left an environmental liability of approximately 500,000 tonnes of metals containing mainly cadmium (Cd), lead (Pb) and zinc (Zn). Soils near the slag contain heavy metals in concentrations above permissible limits, even after years of interruption of metallurgical activity. In this sense, remediation techniques are needed to minimize the effects of metals on contaminated soils and restore ecosystem functionality around the factory. The present work has the objective of evaluating the potential of two legume species (mucuna and bean), inoculated with rhizobia, for use in phytoremediation of contaminated soils. In addition, the occurrence and morphological characteristics of the naturally established rhizobia populations in contaminated soils were evaluated using the two legume species cultivated without inoculation as bait plants. The legumes were cultivated in pots containing soil samples collected at points with increasing distances from the source of contamination, to represent the effect of different concentrations of the metals in the soil, and inoculated with nitrogen - fixing bacteria. Soils presented values of Pb and Cd above the value of investigation, signaling the need for remediation. The plants used presented symptoms of toxicity induced by the excess of heavy metals, however being tolerant, evaluated as a function of biomass production. The populations of native rhizobia of contaminated soils are tolerant and resistant to high concentrations of metals, such as: Cd, Pb and Zn. However, the high concentrations of heavy metals affected the nodulation and consequently the amount of N fixed. The phenotypic characteristics of these isolates showed a very high diversity, unusual, given the amount of isolates. Therefore, further studies with molecular tools to determine which species are able to nodulate in an environment as inhospitable as soils contaminated with heavy metals.No Brasil um importante caso de contaminação ambiental envolvendo metais pesados ocorreu em Santo Amaro, Bahia. Uma empresa de fundição deixou um passivo ambiental de aproximadamente 500.000 toneladas de metais contendo, principalmente, cádmio (Cd), chumbo (Pb) e zinco (Zn). Os solos próximos às escórias contêm metais pesados em concentrações acima dos limites permissíveis, mesmo após anos de interrupção da atividade metalúrgica. Nesse sentindo, técnicas de remediações são necessárias para minimizar os efeitos dos metais nos solos contaminados e recuperar a funcionalidade dos ecossistemas no entorno da fábrica. O presente trabalho tem como objetivo avaliar o potencial de duas espécies de leguminosas (mucuna e feijão-de-porco), inoculadas com rizóbios, para utilização na fitorremediação de solos contaminados. Além disso, foram avaliadas a ocorrência e as características morfológicas das populações de rizóbios naturalmente estabelecidas nos solos contaminados, utilizando as duas espécies de leguminosas cultivadas sem inoculação como plantas iscas. As leguminosas foram cultivadas em vasos contendo amostras de solo coletadas em pontos com distâncias crescentes da fonte de contaminação, para representar o efeito de diferentes concentrações dos metais no solo, e inoculadas com bactérias fixadoras de nitrogênio. Os solos apresentaram valores de Pb e Cd acima do valor de investigação, sinalizando a necessidade de remediação. As plantas utilizadas apresentaram sintomas de toxicidade induzida pelo excesso de metais pesados, no entanto mostrando-se tolerante, avaliadas em função da produção de biomassa. As populações de rizóbios nativos dos solos contaminados são tolerantes e apresentam resistência a elevados teores de metais, como: Cd, Pb e Zn. No entanto os altos teores de metais pesados afetaram a nodulação e consequentemente os teores de nitrogênio nas plantas. As características fenotípicas destes isolados mostraram uma diversidade muito elevada. Estudos subsequentes mais aprofundados com ferramentas moleculares poderão determinar quais espécies são capazes de nodular em ambiente tão inóspito como solos contaminados de metais pesados.Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-09-13T13:24:41Z No. of bitstreams: 1 Jessyca Adriana Gomes Florencio da Silva.pdf: 1173329 bytes, checksum: 3622c22e9f16cdeebda4342094bd851e (MD5)Made available in DSpace on 2019-09-13T13:24:41Z (GMT). No. of bitstreams: 1 Jessyca Adriana Gomes Florencio da Silva.pdf: 1173329 bytes, checksum: 3622c22e9f16cdeebda4342094bd851e (MD5) Previous issue date: 2019-02-22Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência do SoloUFRPEBrasilDepartamento de AgronomiaLeguminosaRizóbioInoculaçãoMicrobiologia do soloMetal pesadoAGRONOMIA::CIENCIA DO SOLOLeguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escóriasUse of inoculated legumes for remediation of soil contaminated with heavy metals by deposition of slaginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8551734419043507650600600600600-6800553879972229205-5919840527232375671-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALJessyca Adriana Gomes Florencio da Silva.pdfJessyca Adriana Gomes Florencio da Silva.pdfapplication/pdf1173329http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8191/2/Jessyca+Adriana+Gomes+Florencio+da+Silva.pdf3622c22e9f16cdeebda4342094bd851eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8191/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/81912019-09-13 10:24:41.194oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:36:39.252458Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
dc.title.alternative.eng.fl_str_mv |
Use of inoculated legumes for remediation of soil contaminated with heavy metals by deposition of slag |
title |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
spellingShingle |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias SILVA, Jessyca Adriana Gomes Florêncio da Leguminosa Rizóbio Inoculação Microbiologia do solo Metal pesado AGRONOMIA::CIENCIA DO SOLO |
title_short |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
title_full |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
title_fullStr |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
title_full_unstemmed |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
title_sort |
Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias |
author |
SILVA, Jessyca Adriana Gomes Florêncio da |
author_facet |
SILVA, Jessyca Adriana Gomes Florêncio da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
FREITAS, Ana Dolores Santiago de |
dc.contributor.advisor-co1.fl_str_mv |
NASCIMENTO, Clístenes Williams Araújo do |
dc.contributor.referee1.fl_str_mv |
FREITAS, Ana Dolores Santiago de |
dc.contributor.referee2.fl_str_mv |
BIONDI, Caroline Miranda |
dc.contributor.referee3.fl_str_mv |
SILVA, Vinícius Santos Gomes da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5807881476724702 |
dc.contributor.author.fl_str_mv |
SILVA, Jessyca Adriana Gomes Florêncio da |
contributor_str_mv |
FREITAS, Ana Dolores Santiago de NASCIMENTO, Clístenes Williams Araújo do FREITAS, Ana Dolores Santiago de BIONDI, Caroline Miranda SILVA, Vinícius Santos Gomes da |
dc.subject.por.fl_str_mv |
Leguminosa Rizóbio Inoculação Microbiologia do solo Metal pesado |
topic |
Leguminosa Rizóbio Inoculação Microbiologia do solo Metal pesado AGRONOMIA::CIENCIA DO SOLO |
dc.subject.cnpq.fl_str_mv |
AGRONOMIA::CIENCIA DO SOLO |
description |
Heavy metals are elements that are potentially toxic to natural ecosystems and to human health, and may originate from natural or anthropogenic sources. In Brazil an important case of environmental contamination involving heavy metals occurred in Santo Amaro, Bahia. One foundry company left an environmental liability of approximately 500,000 tonnes of metals containing mainly cadmium (Cd), lead (Pb) and zinc (Zn). Soils near the slag contain heavy metals in concentrations above permissible limits, even after years of interruption of metallurgical activity. In this sense, remediation techniques are needed to minimize the effects of metals on contaminated soils and restore ecosystem functionality around the factory. The present work has the objective of evaluating the potential of two legume species (mucuna and bean), inoculated with rhizobia, for use in phytoremediation of contaminated soils. In addition, the occurrence and morphological characteristics of the naturally established rhizobia populations in contaminated soils were evaluated using the two legume species cultivated without inoculation as bait plants. The legumes were cultivated in pots containing soil samples collected at points with increasing distances from the source of contamination, to represent the effect of different concentrations of the metals in the soil, and inoculated with nitrogen - fixing bacteria. Soils presented values of Pb and Cd above the value of investigation, signaling the need for remediation. The plants used presented symptoms of toxicity induced by the excess of heavy metals, however being tolerant, evaluated as a function of biomass production. The populations of native rhizobia of contaminated soils are tolerant and resistant to high concentrations of metals, such as: Cd, Pb and Zn. However, the high concentrations of heavy metals affected the nodulation and consequently the amount of N fixed. The phenotypic characteristics of these isolates showed a very high diversity, unusual, given the amount of isolates. Therefore, further studies with molecular tools to determine which species are able to nodulate in an environment as inhospitable as soils contaminated with heavy metals. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-09-13T13:24:41Z |
dc.date.issued.fl_str_mv |
2019-02-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Jessyca Adriana Gomes Florêncio da. Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias. 2019. 68 f. Dissertação (Programa de Pós-Graduação em Ciência do Solo) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8191 |
identifier_str_mv |
SILVA, Jessyca Adriana Gomes Florêncio da. Leguminosas inoculadas com rizobios para remediação de solo contaminado com metais pesados por deposição de escórias. 2019. 68 f. Dissertação (Programa de Pós-Graduação em Ciência do Solo) - Universidade Federal Rural de Pernambuco, Recife. |
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