Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica

Detalhes bibliográficos
Autor(a) principal: RABÊLO, Suzana Vieira
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8170
Resumo: Atemoieira is a hybrid resulting from the crossing of two species of the Annona genus (A. cherimola and A. squamosa) that are known for the richness of biologically active compounds. Due to the increasing number of new cases of cancer each year and aggressive and often inefficient treatments, it is necessary to search for new effective cytotoxic compounds. The use of hyphenated techniques of separation and identification of plant matrix molecules has been highly valued, since they can accelerate searches for these potentially active compounds. Therefore, the objective of this work was to analyze the cytotoxic potential of extracts and phases of different parts of the atemoia plant and to correlate the results with their chemical composition defined by means of different isolation and identification techniques. For this, plant leaves were collected in Petrolina-PE and extracted with hexane followed by methanol to obtain crude extracts hexane (EHB-F) and methanolic (EMB-F), respectively. The EMB-F was subjected to an acid-base extraction to obtain the alkaloidal fraction. The common chromatographic techniques used led to the isolation of a novel alkaloid (here called dehidro-N-oxide anomuricine) and the anonaine, asimilobine, lanuginosine, liriodenine, lisicamine, pronuciferine, stefarine and anomuricine alkaloids whose identifications were made by analysis of spectra masses, uni and bidimensional Nuclear Magnetic Resonance and by comparison with literature data. Anomuricine and dehydro-N-oxide-anomuricine had their fragmentations proposed. In addition, the aerial parts of the plant (mixture of stems and leaves), went through the same procedure that leaves to obtain the hexane crude extracts (EHB-PA), methanol (EMB-PA) and alkaloidal fraction (FAT-PA) which was further subjected to extraction solid-liquid to obtain subfractions - hexane (FAT-Hex), chloroform (FAT-CHCl3), ethyl acetate (FAT-AcOEt) and methanol (FAT-MeOH). All the extracts, fractions and subfractions obtained were tested for their cytotoxicity. The results were compared to liquid chromatography and gas chromatography, both coupled to mass spectrometer (LC-MS and GC-MS, respectively), for chemical correlation with biological potential. All procedures for access to genetic heritage and associated traditional knowledge were carried out and the project was registered with SisGen (Record # ABD9AA7). As result of analysis by LC-MS was possible to identify the presence of anomuricine, dehydro-N-oxide-anomuricine, scoulerine, reticuline, isocorydine, norisocorydine, asimilobine, nornuciferine, liriodenine, anonaine in the EMB-PA. The analysis of EHB-PA by GC-MS led to the identification of 29 compounds, with spatulenol being the major. This extract (EHB-PA) inhibited more than 90% of the growth of glioblastoma cells tested (SF-295). It was also possible to observe that the EMB-PA had its activity potentiated when fractioned, with a difference of up to 41%, approximately. Anomuricin was tested against nine cell lines and presented a cytotoxic potential against leukemic cells (HL-60) with selectivity index of 1.66. The different parts of the hybrid showed high cytotoxic potential and therefore this plant can be considered a promising source of cytotoxically active compounds.
id URPE_35cab056d76bfa0999e239a4d9f7ccae
oai_identifier_str oai:tede2:tede2/8170
network_acronym_str URPE
network_name_str Biblioteca Digital de Teses e Dissertações da UFRPE
repository_id_str
spelling ALMEIDA, Jackson Roberto Guedes da SilvaNUNES, Xirley PereiraCARDOSO, Marcos Veríssimo de OliveiraMATOS, Maria Helena Tavares deLORENZO, Vitor Prateshttp://lattes.cnpq.br/7121516990696112RABÊLO, Suzana Vieira2019-08-22T13:38:17Z2019-02-27RABÊLO, Suzana Vieira. Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica. 2019. 126 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8170Atemoieira is a hybrid resulting from the crossing of two species of the Annona genus (A. cherimola and A. squamosa) that are known for the richness of biologically active compounds. Due to the increasing number of new cases of cancer each year and aggressive and often inefficient treatments, it is necessary to search for new effective cytotoxic compounds. The use of hyphenated techniques of separation and identification of plant matrix molecules has been highly valued, since they can accelerate searches for these potentially active compounds. Therefore, the objective of this work was to analyze the cytotoxic potential of extracts and phases of different parts of the atemoia plant and to correlate the results with their chemical composition defined by means of different isolation and identification techniques. For this, plant leaves were collected in Petrolina-PE and extracted with hexane followed by methanol to obtain crude extracts hexane (EHB-F) and methanolic (EMB-F), respectively. The EMB-F was subjected to an acid-base extraction to obtain the alkaloidal fraction. The common chromatographic techniques used led to the isolation of a novel alkaloid (here called dehidro-N-oxide anomuricine) and the anonaine, asimilobine, lanuginosine, liriodenine, lisicamine, pronuciferine, stefarine and anomuricine alkaloids whose identifications were made by analysis of spectra masses, uni and bidimensional Nuclear Magnetic Resonance and by comparison with literature data. Anomuricine and dehydro-N-oxide-anomuricine had their fragmentations proposed. In addition, the aerial parts of the plant (mixture of stems and leaves), went through the same procedure that leaves to obtain the hexane crude extracts (EHB-PA), methanol (EMB-PA) and alkaloidal fraction (FAT-PA) which was further subjected to extraction solid-liquid to obtain subfractions - hexane (FAT-Hex), chloroform (FAT-CHCl3), ethyl acetate (FAT-AcOEt) and methanol (FAT-MeOH). All the extracts, fractions and subfractions obtained were tested for their cytotoxicity. The results were compared to liquid chromatography and gas chromatography, both coupled to mass spectrometer (LC-MS and GC-MS, respectively), for chemical correlation with biological potential. All procedures for access to genetic heritage and associated traditional knowledge were carried out and the project was registered with SisGen (Record # ABD9AA7). As result of analysis by LC-MS was possible to identify the presence of anomuricine, dehydro-N-oxide-anomuricine, scoulerine, reticuline, isocorydine, norisocorydine, asimilobine, nornuciferine, liriodenine, anonaine in the EMB-PA. The analysis of EHB-PA by GC-MS led to the identification of 29 compounds, with spatulenol being the major. This extract (EHB-PA) inhibited more than 90% of the growth of glioblastoma cells tested (SF-295). It was also possible to observe that the EMB-PA had its activity potentiated when fractioned, with a difference of up to 41%, approximately. Anomuricin was tested against nine cell lines and presented a cytotoxic potential against leukemic cells (HL-60) with selectivity index of 1.66. The different parts of the hybrid showed high cytotoxic potential and therefore this plant can be considered a promising source of cytotoxically active compounds.Atemoieira é um híbrido resultante do cruzamento de duas espécies do gênero Annona (A. cherimola e A. squamosa) que são conhecidas pela riqueza de compostos biologicamente ativos. Devido ao número crescente de novos casos de câncer a cada ano e aos tratamentos agressivos, e muitas vezes ineficientes, faz-se necessário a busca por novos compostos citotóxicos eficazes. O uso de técnicas hifenadas de separação e identificação de moléculas de matrizes vegetais tem sido bastante valorizadas, pois conseguem acelerar as buscas por esses compostos potencialmente ativos. Por isso, o objetivo deste trabalho foi analisar o potencial citotóxico de extratos e fases de diferentes partes da planta atemoia e correlacionar os resultados com sua composição química definida por meio de diferentes técnicas de isolamento e identificação. Para tal, folhas da planta foram coletadas em Petrolina-PE e extraídas com hexano seguido por metanol para obtenção dos extratos brutos hexânico (EHB-F) e metanólico (EMB-F), respectivamente. O EMB-F foi submetido à uma extração ácido base para obtenção da fração alcaloídica. O uso de técnicas cromatográficas comuns levou ao isolamento de um alcaloide inédito (aqui chamado de dehidroanomuricina N-óxido) e dos alcaloides anonaina, asimilobina, lanuginosina, liriodenina, lisicamina, pronuciferina, estefarina e anomuricina, cujas identificações foram feitas por análise de espectros de massas, de Ressonância Magnética Nuclear uni e bidimensionais e por comparação com dados da literatura. Anomuricina e dehidroanomuricina N-óxido tiveram suas fragmentações propostas. Além disso, partes aéreas da planta (talos e folhas), passaram pelo mesmo procedimento que as folhas para obtenção dos extratos brutos hexânico (EHB-PA), metanólico (EMB-PA) e fração alcaloídica (FAT) que foi submetida ainda à extração sólido-líquido para obtenção das suas subfrações - hexânica (FAT-Hex), clorofórmica (FAT-CHCl3), acetato de etila (FAT-AcOEt) e metanólica (FAT-MeOH). Todas as fases, frações e subfrações obtidas foram testadas quanto à sua citotoxicidade. Os resultados foram comparados com estudos de cromatografia líquida e cromatografia gasosa, ambas acopladas a espetrômetro de massas (CL-EM e CG-EM, respectivamente), para correlação química com potencial biológico. Todos os procedimentos de acesso ao patrimônio genético e conhecimentos tradicionais associados foram realizados e o projeto foi registrado no SisGen (Registro #ABD9AA7). Como resultado das análises por CL-EM foi possível identificar a presença de anomuricina, dehidroanomuricina N-óxido, esculerina, reticulina, isocoridina, norisocoridina, asimilobina, nornuciferina, anonaina e liriodenina no EMB-PA. A análise do EHB-PA por CG-EM levou a identificação de 29 compostos, sendo o espatulenol o majoritário. Este extrato (EHB-PA) inibiu mais de 90% do crescimento das células de glioblastoma testadas (SF-295). Foi possível observar ainda, que o EMB-PA teve sua atividade potencializada quando fracionada, com diferença de até 41%, aproximadamente. Anomuricina foi testada diante de nove linhagens celulares e apresentou potencial citotóxico diante de células leucêmicas (HL-60) com índice de seletividade de 1,66. As diferentes partes do híbrido apresentaram alto potencial citotóxico e por isso esta planta pode ser considerada uma fonte promissora de compostos citotoxicamente ativos.Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-08-22T13:38:17Z No. of bitstreams: 1 Suzana Vieira Rabelo.pdf: 4359773 bytes, checksum: 30f89c26cffd9884839e3932b3277ac8 (MD5)Made available in DSpace on 2019-08-22T13:38:17Z (GMT). No. of bitstreams: 1 Suzana Vieira Rabelo.pdf: 4359773 bytes, checksum: 30f89c26cffd9884839e3932b3277ac8 (MD5) Previous issue date: 2019-02-27Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biotecnologia (Renorbio)UFRPEBrasilRede Nordeste de BiotecnologiaAtemoiaAnnona cherimolaAnnona squamosaTécnica hifenadaAtividade citotóxicaOUTROS::CIENCIASUso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis7794227690756777355600600600600-81045765884522764216209957791494323825-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALSuzana Vieira Rabelo.pdfSuzana Vieira Rabelo.pdfapplication/pdf4359773http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8170/2/Suzana+Vieira+Rabelo.pdf30f89c26cffd9884839e3932b3277ac8MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8170/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/81702019-08-22 10:38:17.961oai:tede2:tede2/8170Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:36:37.569049Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
title Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
spellingShingle Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
RABÊLO, Suzana Vieira
Atemoia
Annona cherimola
Annona squamosa
Técnica hifenada
Atividade citotóxica
OUTROS::CIENCIAS
title_short Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
title_full Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
title_fullStr Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
title_full_unstemmed Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
title_sort Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica
author RABÊLO, Suzana Vieira
author_facet RABÊLO, Suzana Vieira
author_role author
dc.contributor.advisor1.fl_str_mv ALMEIDA, Jackson Roberto Guedes da Silva
dc.contributor.referee1.fl_str_mv NUNES, Xirley Pereira
dc.contributor.referee2.fl_str_mv CARDOSO, Marcos Veríssimo de Oliveira
dc.contributor.referee3.fl_str_mv MATOS, Maria Helena Tavares de
dc.contributor.referee4.fl_str_mv LORENZO, Vitor Prates
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7121516990696112
dc.contributor.author.fl_str_mv RABÊLO, Suzana Vieira
contributor_str_mv ALMEIDA, Jackson Roberto Guedes da Silva
NUNES, Xirley Pereira
CARDOSO, Marcos Veríssimo de Oliveira
MATOS, Maria Helena Tavares de
LORENZO, Vitor Prates
dc.subject.por.fl_str_mv Atemoia
Annona cherimola
Annona squamosa
Técnica hifenada
Atividade citotóxica
topic Atemoia
Annona cherimola
Annona squamosa
Técnica hifenada
Atividade citotóxica
OUTROS::CIENCIAS
dc.subject.cnpq.fl_str_mv OUTROS::CIENCIAS
description Atemoieira is a hybrid resulting from the crossing of two species of the Annona genus (A. cherimola and A. squamosa) that are known for the richness of biologically active compounds. Due to the increasing number of new cases of cancer each year and aggressive and often inefficient treatments, it is necessary to search for new effective cytotoxic compounds. The use of hyphenated techniques of separation and identification of plant matrix molecules has been highly valued, since they can accelerate searches for these potentially active compounds. Therefore, the objective of this work was to analyze the cytotoxic potential of extracts and phases of different parts of the atemoia plant and to correlate the results with their chemical composition defined by means of different isolation and identification techniques. For this, plant leaves were collected in Petrolina-PE and extracted with hexane followed by methanol to obtain crude extracts hexane (EHB-F) and methanolic (EMB-F), respectively. The EMB-F was subjected to an acid-base extraction to obtain the alkaloidal fraction. The common chromatographic techniques used led to the isolation of a novel alkaloid (here called dehidro-N-oxide anomuricine) and the anonaine, asimilobine, lanuginosine, liriodenine, lisicamine, pronuciferine, stefarine and anomuricine alkaloids whose identifications were made by analysis of spectra masses, uni and bidimensional Nuclear Magnetic Resonance and by comparison with literature data. Anomuricine and dehydro-N-oxide-anomuricine had their fragmentations proposed. In addition, the aerial parts of the plant (mixture of stems and leaves), went through the same procedure that leaves to obtain the hexane crude extracts (EHB-PA), methanol (EMB-PA) and alkaloidal fraction (FAT-PA) which was further subjected to extraction solid-liquid to obtain subfractions - hexane (FAT-Hex), chloroform (FAT-CHCl3), ethyl acetate (FAT-AcOEt) and methanol (FAT-MeOH). All the extracts, fractions and subfractions obtained were tested for their cytotoxicity. The results were compared to liquid chromatography and gas chromatography, both coupled to mass spectrometer (LC-MS and GC-MS, respectively), for chemical correlation with biological potential. All procedures for access to genetic heritage and associated traditional knowledge were carried out and the project was registered with SisGen (Record # ABD9AA7). As result of analysis by LC-MS was possible to identify the presence of anomuricine, dehydro-N-oxide-anomuricine, scoulerine, reticuline, isocorydine, norisocorydine, asimilobine, nornuciferine, liriodenine, anonaine in the EMB-PA. The analysis of EHB-PA by GC-MS led to the identification of 29 compounds, with spatulenol being the major. This extract (EHB-PA) inhibited more than 90% of the growth of glioblastoma cells tested (SF-295). It was also possible to observe that the EMB-PA had its activity potentiated when fractioned, with a difference of up to 41%, approximately. Anomuricin was tested against nine cell lines and presented a cytotoxic potential against leukemic cells (HL-60) with selectivity index of 1.66. The different parts of the hybrid showed high cytotoxic potential and therefore this plant can be considered a promising source of cytotoxically active compounds.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-22T13:38:17Z
dc.date.issued.fl_str_mv 2019-02-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv RABÊLO, Suzana Vieira. Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica. 2019. 126 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8170
identifier_str_mv RABÊLO, Suzana Vieira. Uso de técnicas hifenadas (CLAE-DAD/CL-EM) na identificação de metabólitos secundários da atemoia (Annona cherimola x Annona squamosa) e avaliação da atividade citotóxica. 2019. 126 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8170
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 7794227690756777355
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -8104576588452276421
dc.relation.cnpq.fl_str_mv 6209957791494323825
dc.relation.sponsorship.fl_str_mv -2555911436985713659
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biotecnologia (Renorbio)
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Rede Nordeste de Biotecnologia
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRPE
instname:Universidade Federal Rural de Pernambuco (UFRPE)
instacron:UFRPE
instname_str Universidade Federal Rural de Pernambuco (UFRPE)
instacron_str UFRPE
institution UFRPE
reponame_str Biblioteca Digital de Teses e Dissertações da UFRPE
collection Biblioteca Digital de Teses e Dissertações da UFRPE
bitstream.url.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8170/2/Suzana+Vieira+Rabelo.pdf
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8170/1/license.txt
bitstream.checksum.fl_str_mv 30f89c26cffd9884839e3932b3277ac8
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)
repository.mail.fl_str_mv bdtd@ufrpe.br ||bdtd@ufrpe.br
_version_ 1810102259852247040