Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6549 |
Resumo: | Bacteria with probiotic effect have been used in marine shrimp production like antibiotics substitution, contributing to the health of the host, by antagonistic action to pathogenic micro-organisms or by competition for space and nutrients, and improving appetite and lead the further growth of the animals. When isolated from the host itself is safe to use, being able to adhere and colonize the intestine. The objective was to isolate probiotic bacteria from intestine of juvenile Litopenaeus vannamei and evaluate the effects of probiotic administration in the performance of cultivated animals experimental challenged infection. Intestinal bacteria were from cultivated shrimp in oligohaline water and saltwalter in dry and rainy seasons, and confronted with Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus and Aeromonas hydrophila. Bacteria showed antimicrobial effect have been identified by molecular biology. Twenty-nine isolates showed antagonistic effect at least one of the tested pathogens. The species that most occurred was Bacillus cereus, the producing of the largest zones of inhibition to the V. alginolyticus and V. vulnificus. The bacterium Citrobacter freundii, from oligohaline water showed the best antimicrobial effect before the four pathogens. The probiotic Bacillus cereus was tested in diet for marine shrimp post-larvae against challenge with V. parahaemolyticus, and V. alginolyticus which evaluated the growth performance of animals, colonization capacity of the probiotic bacteria, pathogens count and histopathological lesions. The use of probiotic had no effect on animals survival, but not for treatments which was used probiotic had a lower weight gain. Animals fed dietary supplementation of probiotic had lower pathogens count those fed without the use. Histopathological lesions were observed in organs and tissues of animals. It can be concluded that it was possible to isolate bacteria having probiotic effect of marine shrimp intestine, wherein the strain Bacillus cereus demonstrated high capacity to colonize the host itself, causing a significant reduction of pathogens. |
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MENDES, Emiko ShinozakiSANTOS, Juliana Ferreira dosMENDES, Paulo de PaulaSANTOS, Fernando Leandro dosPEREIRA, Alitiene Moura Lemoshttp://lattes.cnpq.br/4644906989773828VIDAL, Juliana Maria Aderaldo2017-02-23T14:03:29Z2015-07-27VIDAL, Juliana Maria Aderaldo. Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931). 2015. 79 f. Tese (Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6549Bacteria with probiotic effect have been used in marine shrimp production like antibiotics substitution, contributing to the health of the host, by antagonistic action to pathogenic micro-organisms or by competition for space and nutrients, and improving appetite and lead the further growth of the animals. When isolated from the host itself is safe to use, being able to adhere and colonize the intestine. The objective was to isolate probiotic bacteria from intestine of juvenile Litopenaeus vannamei and evaluate the effects of probiotic administration in the performance of cultivated animals experimental challenged infection. Intestinal bacteria were from cultivated shrimp in oligohaline water and saltwalter in dry and rainy seasons, and confronted with Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus and Aeromonas hydrophila. Bacteria showed antimicrobial effect have been identified by molecular biology. Twenty-nine isolates showed antagonistic effect at least one of the tested pathogens. The species that most occurred was Bacillus cereus, the producing of the largest zones of inhibition to the V. alginolyticus and V. vulnificus. The bacterium Citrobacter freundii, from oligohaline water showed the best antimicrobial effect before the four pathogens. The probiotic Bacillus cereus was tested in diet for marine shrimp post-larvae against challenge with V. parahaemolyticus, and V. alginolyticus which evaluated the growth performance of animals, colonization capacity of the probiotic bacteria, pathogens count and histopathological lesions. The use of probiotic had no effect on animals survival, but not for treatments which was used probiotic had a lower weight gain. Animals fed dietary supplementation of probiotic had lower pathogens count those fed without the use. Histopathological lesions were observed in organs and tissues of animals. It can be concluded that it was possible to isolate bacteria having probiotic effect of marine shrimp intestine, wherein the strain Bacillus cereus demonstrated high capacity to colonize the host itself, causing a significant reduction of pathogens.Bactérias com efeito probiótico contribuem para saúde de camarões marinhos em cultivo, em substituição aos antibióticos, exercendo ação antagonista à micro-organismos patogênicos ou por competição por espaço e nutrientes, além de melhorar o apetite levando a um maior crescimento dos animais. Quando isolados do próprio camarão seu uso é seguro, por serem capazes de aderir ou colonizar o intestino destes. Neste sentido, objetivou-se isolar bactérias probióticas do intestino de juvenis de Litopenaeus vannamei e avaliar os efeitos da sua administração sobre o desempenho dos animais cultivados frente infecção experimental. Foram isoladas bactérias do intestino de camarão cultivado em águas oligohalina e salgada, em períodos seco e chuvoso, e confrontadas com Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus e Aeromonas hydrophila, em testes in vitro. As bactérias que produziram efeito antimicrobiano foram identificadas através de biologia molecular.Vinte e nove isolados apresentaram efeito antagônico a pelo menos um dos patógenos testados. A espécie que mais ocorreu foi Bacillus cereus, produzindo os maiores halos de inibição frente ao V. alginolyticus e V. vulnificus. A bactéria Citrobacter freundii, proveniente de água oligohalina apresentou melhor efeito antimicrobiano, perante os quatro patógenos. O probiótico Bacillus cereus foi testado em ração para pós-larvas de L. vannamei, frente a desafio com V. parahaemolyticus, e V. alginolyticus quando avaliou-se o desempenho zootécnico dos animais, a capacidade de colonização das bactérias probióticas, contagem de patógenos e lesões histopatológicas. O uso do probiótico não influenciou nas taxas de sobrevivência dos animais, porém nos tratamentos em que não se utilizou probiótico houve menor ganho de peso. Os animais que receberam ração suplementada de probiótico, tiveram contagem de patógenos inferior aqueles alimentados sem o uso. Não foram observadas lesões histopatológicas nos órgãos e tecidos dos animais. Concluiu-se que é possível isolar bactérias com efeito probiótico do intestino do camarão marinho, sendo que a cepa de Bacillus cereus demonstrou maior capacidade de colonizar o próprio hospedeiro, diminuindo os patógenos.Submitted by (edna.saturno@ufrpe.br) on 2017-02-23T14:03:29Z No. of bitstreams: 1 Juliana Maria Aderaldo Vidal.pdf: 784041 bytes, checksum: f927beb63f5c3c304d4098214b0dd629 (MD5)Made available in DSpace on 2017-02-23T14:03:29Z (GMT). No. of bitstreams: 1 Juliana Maria Aderaldo Vidal.pdf: 784041 bytes, checksum: f927beb63f5c3c304d4098214b0dd629 (MD5) Previous issue date: 2015-07-27application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Recursos Pesqueiros e AquiculturaUFRPEBrasilDepartamento de Pesca e AquiculturaBactéria probióticaTeste de antagonismoBacillus cereusInfecção experimentalCamarão marinhoProbiotic bacteriaAntagonism testExperimental infectionMarine shrimpCIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCABactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis80217415640343225476006006007231936942857037408-6131750198709519811info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6549/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALJuliana Maria Aderaldo Vidal.pdfJuliana Maria Aderaldo Vidal.pdfapplication/pdf784041http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6549/2/Juliana+Maria+Aderaldo+Vidal.pdff927beb63f5c3c304d4098214b0dd629MD52tede2/65492017-02-23 11:03:29.052oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:34:30.086158Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
title |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
spellingShingle |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) VIDAL, Juliana Maria Aderaldo Bactéria probiótica Teste de antagonismo Bacillus cereus Infecção experimental Camarão marinho Probiotic bacteria Antagonism test Experimental infection Marine shrimp CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
title_short |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
title_full |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
title_fullStr |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
title_full_unstemmed |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
title_sort |
Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931) |
author |
VIDAL, Juliana Maria Aderaldo |
author_facet |
VIDAL, Juliana Maria Aderaldo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
MENDES, Emiko Shinozaki |
dc.contributor.referee1.fl_str_mv |
SANTOS, Juliana Ferreira dos |
dc.contributor.referee2.fl_str_mv |
MENDES, Paulo de Paula |
dc.contributor.referee3.fl_str_mv |
SANTOS, Fernando Leandro dos |
dc.contributor.referee4.fl_str_mv |
PEREIRA, Alitiene Moura Lemos |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4644906989773828 |
dc.contributor.author.fl_str_mv |
VIDAL, Juliana Maria Aderaldo |
contributor_str_mv |
MENDES, Emiko Shinozaki SANTOS, Juliana Ferreira dos MENDES, Paulo de Paula SANTOS, Fernando Leandro dos PEREIRA, Alitiene Moura Lemos |
dc.subject.por.fl_str_mv |
Bactéria probiótica Teste de antagonismo Bacillus cereus Infecção experimental Camarão marinho |
topic |
Bactéria probiótica Teste de antagonismo Bacillus cereus Infecção experimental Camarão marinho Probiotic bacteria Antagonism test Experimental infection Marine shrimp CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
dc.subject.eng.fl_str_mv |
Probiotic bacteria Antagonism test Experimental infection Marine shrimp |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
description |
Bacteria with probiotic effect have been used in marine shrimp production like antibiotics substitution, contributing to the health of the host, by antagonistic action to pathogenic micro-organisms or by competition for space and nutrients, and improving appetite and lead the further growth of the animals. When isolated from the host itself is safe to use, being able to adhere and colonize the intestine. The objective was to isolate probiotic bacteria from intestine of juvenile Litopenaeus vannamei and evaluate the effects of probiotic administration in the performance of cultivated animals experimental challenged infection. Intestinal bacteria were from cultivated shrimp in oligohaline water and saltwalter in dry and rainy seasons, and confronted with Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus and Aeromonas hydrophila. Bacteria showed antimicrobial effect have been identified by molecular biology. Twenty-nine isolates showed antagonistic effect at least one of the tested pathogens. The species that most occurred was Bacillus cereus, the producing of the largest zones of inhibition to the V. alginolyticus and V. vulnificus. The bacterium Citrobacter freundii, from oligohaline water showed the best antimicrobial effect before the four pathogens. The probiotic Bacillus cereus was tested in diet for marine shrimp post-larvae against challenge with V. parahaemolyticus, and V. alginolyticus which evaluated the growth performance of animals, colonization capacity of the probiotic bacteria, pathogens count and histopathological lesions. The use of probiotic had no effect on animals survival, but not for treatments which was used probiotic had a lower weight gain. Animals fed dietary supplementation of probiotic had lower pathogens count those fed without the use. Histopathological lesions were observed in organs and tissues of animals. It can be concluded that it was possible to isolate bacteria having probiotic effect of marine shrimp intestine, wherein the strain Bacillus cereus demonstrated high capacity to colonize the host itself, causing a significant reduction of pathogens. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-07-27 |
dc.date.accessioned.fl_str_mv |
2017-02-23T14:03:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VIDAL, Juliana Maria Aderaldo. Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931). 2015. 79 f. Tese (Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6549 |
identifier_str_mv |
VIDAL, Juliana Maria Aderaldo. Bactérias com potencial probiótico isoladas do intestino de camarão marinho Litopenaeus vannamei (Boone, 1931). 2015. 79 f. Tese (Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6549 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal Rural de Pernambuco |
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Universidade Federal Rural de Pernambuco |
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