Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco

Detalhes bibliográficos
Autor(a) principal: BORGES, Jonas de Melo
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6211
Resumo: The objective of this study was to determine the occurrence of infection with Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in buffaloes in the State of Pernambuco, Brazil and identify possible risk factors associated with infection. Biological samples were collected (cervico vaginal mucus and shaved prepucial) of 113 animals, coming from 8 properties in different regions of the state. The biological material collected was transferred into phosphate buffered saline (PBS) and inoculated in the specific transport, Lander for diagnosis of C. fetus subsp. venerealis and Diamond for T. fetus subsequently. For the diagnosis of infection by Campylobacter fetus subsp. venrealis and Tritrichomonas foetus the samples were submitted to Polymerase Chain Reaction ( PCR) grown in Columbia agar plus antibiotics and Diamond, respectively. There was an occurrence of 1.8% (2/113 ; I.C. 0.2 to 6.2 % ) of positive animals in the microbiological examination with confirmation by PCR, for C. fetus subsp. venerealis. It was observed that 100% of positive samples were from two (2) males from the same herd. No animals were positive for T. foetus. It was not possible to identify risk factors associated with infection. This is the first report of infection with C. fetus subsp. venerealis in buffaloes in Brazil. Despite the low occurrence it is recommended that control measures are adopted, in order to prevent the spread of the agent to other herds.
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spelling PINHEIRO JUNIOR, José WiltonBRANDESPIM, Daniel FrigugliettiCARNEIRO, Gustavo Ferrerhttp://lattes.cnpq.br/9844322841872677BORGES, Jonas de Melo2017-02-07T13:04:40Z2016-07-20BORGES, Jonas de Melo. Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco. 2016. 58 f. Dissertação (Programa de Pós-Graduação em Sanidade e Reprodução de Ruminantes) - Universidade Federal Rural de Pernambuco, Garanhuns.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6211The objective of this study was to determine the occurrence of infection with Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in buffaloes in the State of Pernambuco, Brazil and identify possible risk factors associated with infection. Biological samples were collected (cervico vaginal mucus and shaved prepucial) of 113 animals, coming from 8 properties in different regions of the state. The biological material collected was transferred into phosphate buffered saline (PBS) and inoculated in the specific transport, Lander for diagnosis of C. fetus subsp. venerealis and Diamond for T. fetus subsequently. For the diagnosis of infection by Campylobacter fetus subsp. venrealis and Tritrichomonas foetus the samples were submitted to Polymerase Chain Reaction ( PCR) grown in Columbia agar plus antibiotics and Diamond, respectively. There was an occurrence of 1.8% (2/113 ; I.C. 0.2 to 6.2 % ) of positive animals in the microbiological examination with confirmation by PCR, for C. fetus subsp. venerealis. It was observed that 100% of positive samples were from two (2) males from the same herd. No animals were positive for T. foetus. It was not possible to identify risk factors associated with infection. This is the first report of infection with C. fetus subsp. venerealis in buffaloes in Brazil. Despite the low occurrence it is recommended that control measures are adopted, in order to prevent the spread of the agent to other herds.Objetivou-se com este estudo determinar a ocorrência da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no Estado de Pernambuco, Brasil e identificar os possíveis fatores de risco associados às infecções. Foram coletadas amostras biológicas (muco cérvico-vaginal e raspado prepucial) de 113 animais, procedentes de 8 propriedades, de diferentes regiões do Estado. O material biológico coletado foi transferido para solução salina tamponada (PBS) e posteriormente inoculado nos meios de transporte específicos, Lander para diagnóstico de C. fetus subsp. venerealis e Diamond para T. foetus. Para o diagnóstico das infecções por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus as amostras foram cultivadas em meio ágar columbia acrescido de antibiótico e Diamond, respectivamente. Posteriormente as amostras de PBS foram submetidas à Reação em Cadeia da Polimerase (PCR). Para pesquisa de C. fetus subsp. venerealis, observou-se uma ocorrência de 1,8% (2/113; I.C. 0,2 – 6,2%) de animais positivos no exame microbiológico com confirmação pela PCR. Em relação à procedência, observou-se que 100% das amostras positivas pertenciam a dois machos do mesmo rebanho. Na pesquisa de T. foetus nenhum animal foi positivo. Não foi possível identificar os fatores de risco associados às infecções. Este é o primeiro registro da infecção por C. fetus subsp. venerealis em búfalos no Brasil. Apesar da baixa ocorrência recomenda-se que medidas de controle sejam adotadas, com o intuito de evitar a disseminação do agente para outros rebanhos.Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-02-07T13:04:40Z No. of bitstreams: 1 Jonas de Melo Borges.pdf: 1057256 bytes, checksum: 5a36d4a5894648ecc348d8146d19bca1 (MD5)Made available in DSpace on 2017-02-07T13:04:40Z (GMT). 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dc.title.por.fl_str_mv Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
title Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
spellingShingle Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
BORGES, Jonas de Melo
Infecção
Búfalo
Campilobacteriose genital
Tricomonose genital
Infection
Buffaloes
Genital campylobacteriosis
Genital trichomoniasis
MEDICINA VETERINARIA::REPRODUCAO ANIMAL
title_short Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
title_full Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
title_fullStr Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
title_full_unstemmed Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
title_sort Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco
author BORGES, Jonas de Melo
author_facet BORGES, Jonas de Melo
author_role author
dc.contributor.advisor1.fl_str_mv PINHEIRO JUNIOR, José Wilton
dc.contributor.referee1.fl_str_mv BRANDESPIM, Daniel Friguglietti
dc.contributor.referee2.fl_str_mv CARNEIRO, Gustavo Ferrer
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9844322841872677
dc.contributor.author.fl_str_mv BORGES, Jonas de Melo
contributor_str_mv PINHEIRO JUNIOR, José Wilton
BRANDESPIM, Daniel Friguglietti
CARNEIRO, Gustavo Ferrer
dc.subject.por.fl_str_mv Infecção
Búfalo
Campilobacteriose genital
Tricomonose genital
topic Infecção
Búfalo
Campilobacteriose genital
Tricomonose genital
Infection
Buffaloes
Genital campylobacteriosis
Genital trichomoniasis
MEDICINA VETERINARIA::REPRODUCAO ANIMAL
dc.subject.eng.fl_str_mv Infection
Buffaloes
Genital campylobacteriosis
Genital trichomoniasis
dc.subject.cnpq.fl_str_mv MEDICINA VETERINARIA::REPRODUCAO ANIMAL
description The objective of this study was to determine the occurrence of infection with Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in buffaloes in the State of Pernambuco, Brazil and identify possible risk factors associated with infection. Biological samples were collected (cervico vaginal mucus and shaved prepucial) of 113 animals, coming from 8 properties in different regions of the state. The biological material collected was transferred into phosphate buffered saline (PBS) and inoculated in the specific transport, Lander for diagnosis of C. fetus subsp. venerealis and Diamond for T. fetus subsequently. For the diagnosis of infection by Campylobacter fetus subsp. venrealis and Tritrichomonas foetus the samples were submitted to Polymerase Chain Reaction ( PCR) grown in Columbia agar plus antibiotics and Diamond, respectively. There was an occurrence of 1.8% (2/113 ; I.C. 0.2 to 6.2 % ) of positive animals in the microbiological examination with confirmation by PCR, for C. fetus subsp. venerealis. It was observed that 100% of positive samples were from two (2) males from the same herd. No animals were positive for T. foetus. It was not possible to identify risk factors associated with infection. This is the first report of infection with C. fetus subsp. venerealis in buffaloes in Brazil. Despite the low occurrence it is recommended that control measures are adopted, in order to prevent the spread of the agent to other herds.
publishDate 2016
dc.date.issued.fl_str_mv 2016-07-20
dc.date.accessioned.fl_str_mv 2017-02-07T13:04:40Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv BORGES, Jonas de Melo. Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco. 2016. 58 f. Dissertação (Programa de Pós-Graduação em Sanidade e Reprodução de Ruminantes) - Universidade Federal Rural de Pernambuco, Garanhuns.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6211
identifier_str_mv BORGES, Jonas de Melo. Estudo epidemiológico da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco. 2016. 58 f. Dissertação (Programa de Pós-Graduação em Sanidade e Reprodução de Ruminantes) - Universidade Federal Rural de Pernambuco, Garanhuns.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6211
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dc.publisher.department.fl_str_mv Unidade Acadêmica de Garanhuns
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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