Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6657 |
Resumo: | Fusarium guttiforme (sin. Fusarium subglutinans f. sp. ananas) is the causal agent of fusariosis on pineapple (Ananas comosus). This disease, considered the most important to this crop, has the exudation of gum as the most common symptom. Most of the published studies focus on the evaluation of the resistance of pineapple varieties against F. guttiforme. The estimative of genetic diversity in Fusarium populations is fundamental to understand the population structure, the movement of pathogen genetic material between producing regions, as well as understanding the processes that lead to resistance against pesticides and overcoming genetics resistance of pineapple cultivars. This fungus, which can be found in most regions of the country, does not have a known teleomorph and belongs to the Gibberella fujikuroi species complex, a group with heterogeneous characteristics. This study aimed: a) to obtain a collection of Fusarium isolates associated with pineapple fusariosis in the country; b) to reconstruct the phylogeny of Fusarium isolates that causes fusariosis on pineapple from Northeast and Southeast regions; c) to test the pathogenicity of isolates. DNA sequences of translation elongation fator 1-α (tef1) and calmodulin (cmd) were analyzed, and the history of the alleles was inferred by phylogenetic methods. Tef1 gene sequences were used to estimate the number of haplotypes from isolates collected in different regions. It was observed that the F. guttiforme is the causal agent of fusariosis on pineapple in Brazil, and the tef1 gene allowed a better infraspecific group distinction than cmd gene. |
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LIMA, Cristiano SouzaPFENNING, Ludwig HeinrichVENTURA, José AiresMICHEREFF, Sami JorgeMEDEIROS, Érica Valente deSOUZA, Breno Oliveira dehttp://lattes.cnpq.br/9937948437311659TSUJI, Susan Satie2017-03-23T15:24:11Z2012-02-29TSUJI, Susan Satie. Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil. 2012. 51 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6657Fusarium guttiforme (sin. Fusarium subglutinans f. sp. ananas) is the causal agent of fusariosis on pineapple (Ananas comosus). This disease, considered the most important to this crop, has the exudation of gum as the most common symptom. Most of the published studies focus on the evaluation of the resistance of pineapple varieties against F. guttiforme. The estimative of genetic diversity in Fusarium populations is fundamental to understand the population structure, the movement of pathogen genetic material between producing regions, as well as understanding the processes that lead to resistance against pesticides and overcoming genetics resistance of pineapple cultivars. This fungus, which can be found in most regions of the country, does not have a known teleomorph and belongs to the Gibberella fujikuroi species complex, a group with heterogeneous characteristics. This study aimed: a) to obtain a collection of Fusarium isolates associated with pineapple fusariosis in the country; b) to reconstruct the phylogeny of Fusarium isolates that causes fusariosis on pineapple from Northeast and Southeast regions; c) to test the pathogenicity of isolates. DNA sequences of translation elongation fator 1-α (tef1) and calmodulin (cmd) were analyzed, and the history of the alleles was inferred by phylogenetic methods. Tef1 gene sequences were used to estimate the number of haplotypes from isolates collected in different regions. It was observed that the F. guttiforme is the causal agent of fusariosis on pineapple in Brazil, and the tef1 gene allowed a better infraspecific group distinction than cmd gene.A fusariose do abacaxizeiro (Ananas comosus) tem como agente etiológico o fungo Fusarium guttiforme (sin. Fusarium subglutinans f. sp. ananas). Esta doença, considerada a mais importante da cultura, tem como sintoma a exsudação de goma, sendo conhecida também como gomose ou resinose fúngica. A maior parte dos estudos publicados avaliou a resistência de variedades de abacaxizeiro a F. guttiforme. A estimativa da diversidade genética em populações de Fusarium é fundamental para o entendimento da estrutura populacional, do movimento de material genético do patógeno entre regiões produtoras através da disseminação do mesmo, bem como do entendimento dos processos que levam a resistência a defensivos e superação da resistência genética de cultivares de abacaxi. Esse fungo, que se encontra disseminado por todas as regiões produtoras do país, ainda não possui o teleomorfo conhecido e pertence ao complexo Gibberella fujikuroi, um grupo com características heterogêneas. O presente trabalho teve como objetivos: a) obter uma coleção de isolados de Fusarium associados à fusariose do abacaxizeiro no país; b) avaliar a filogenia de isolados de Fusarium das regiões Nordeste e Sudeste; c) testar a patogenicidade dos isolados obtidos. Foram analisadas sequências parciais de DNA dos genes fator de elongação 1-α (tef1) e calmodulina (cmd), inferindo-se a história dos alelos através de métodos filogenéticos. Sequências parciais do gene tef1 foram utilizadas para estimar o número de haplótipos dos isolados coletados em diferentes regiões produtoras. Observou-se que a espécie F. guttiforme é o agente causal da gomose do abacaxizeiro no Brasil e que o gene tef1 possibilitou uma melhor distinção de grupos infraespecíficos que o gene cmd.Submitted by (lucia.rodrigues@ufrpe.br) on 2017-03-23T15:24:11Z No. of bitstreams: 1 Susan Satie Tsuji.pdf: 801633 bytes, checksum: f830ce7fba5b8d7999b6897bb595841a (MD5)Made available in DSpace on 2017-03-23T15:24:11Z (GMT). No. of bitstreams: 1 Susan Satie Tsuji.pdf: 801633 bytes, checksum: f830ce7fba5b8d7999b6897bb595841a (MD5) Previous issue date: 2012-02-29Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em FitopatologiaUFRPEBrasilDepartamento de AgronomiaFusarium guttiformeFusarium ananatumFilogeniaAnanas comosusPhylogenyFITOSSANIDADE::FITOPATOLOGIAAnálise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1343367238723626701600600600600-6800553879972229205-6207026424523013504-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6657/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALSusan Satie Tsuji.pdfSusan Satie Tsuji.pdfapplication/pdf801633http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6657/2/Susan+Satie+Tsuji.pdff830ce7fba5b8d7999b6897bb595841aMD52tede2/66572017-03-23 12:24:11.354oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:34:38.796951Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
title |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
spellingShingle |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil TSUJI, Susan Satie Fusarium guttiforme Fusarium ananatum Filogenia Ananas comosus Phylogeny FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
title_full |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
title_fullStr |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
title_full_unstemmed |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
title_sort |
Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil |
author |
TSUJI, Susan Satie |
author_facet |
TSUJI, Susan Satie |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
LIMA, Cristiano Souza |
dc.contributor.advisor-co1.fl_str_mv |
PFENNING, Ludwig Heinrich |
dc.contributor.advisor-co2.fl_str_mv |
VENTURA, José Aires |
dc.contributor.referee1.fl_str_mv |
MICHEREFF, Sami Jorge |
dc.contributor.referee2.fl_str_mv |
MEDEIROS, Érica Valente de |
dc.contributor.referee3.fl_str_mv |
SOUZA, Breno Oliveira de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9937948437311659 |
dc.contributor.author.fl_str_mv |
TSUJI, Susan Satie |
contributor_str_mv |
LIMA, Cristiano Souza PFENNING, Ludwig Heinrich VENTURA, José Aires MICHEREFF, Sami Jorge MEDEIROS, Érica Valente de SOUZA, Breno Oliveira de |
dc.subject.por.fl_str_mv |
Fusarium guttiforme Fusarium ananatum Filogenia Ananas comosus Phylogeny |
topic |
Fusarium guttiforme Fusarium ananatum Filogenia Ananas comosus Phylogeny FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.cnpq.fl_str_mv |
FITOSSANIDADE::FITOPATOLOGIA |
description |
Fusarium guttiforme (sin. Fusarium subglutinans f. sp. ananas) is the causal agent of fusariosis on pineapple (Ananas comosus). This disease, considered the most important to this crop, has the exudation of gum as the most common symptom. Most of the published studies focus on the evaluation of the resistance of pineapple varieties against F. guttiforme. The estimative of genetic diversity in Fusarium populations is fundamental to understand the population structure, the movement of pathogen genetic material between producing regions, as well as understanding the processes that lead to resistance against pesticides and overcoming genetics resistance of pineapple cultivars. This fungus, which can be found in most regions of the country, does not have a known teleomorph and belongs to the Gibberella fujikuroi species complex, a group with heterogeneous characteristics. This study aimed: a) to obtain a collection of Fusarium isolates associated with pineapple fusariosis in the country; b) to reconstruct the phylogeny of Fusarium isolates that causes fusariosis on pineapple from Northeast and Southeast regions; c) to test the pathogenicity of isolates. DNA sequences of translation elongation fator 1-α (tef1) and calmodulin (cmd) were analyzed, and the history of the alleles was inferred by phylogenetic methods. Tef1 gene sequences were used to estimate the number of haplotypes from isolates collected in different regions. It was observed that the F. guttiforme is the causal agent of fusariosis on pineapple in Brazil, and the tef1 gene allowed a better infraspecific group distinction than cmd gene. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-29 |
dc.date.accessioned.fl_str_mv |
2017-03-23T15:24:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TSUJI, Susan Satie. Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil. 2012. 51 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6657 |
identifier_str_mv |
TSUJI, Susan Satie. Análise filogenética e patogênica do agente causal da fusariose do abacaxizeiro no Brasil. 2012. 51 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6657 |
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por |
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Universidade Federal Rural de Pernambuco |
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Universidade Federal Rural de Pernambuco |
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