Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.

Detalhes bibliográficos
Autor(a) principal: SILVA, Michelangelo de Oliveira
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5273
Resumo: The exploration and understanding of the mechanisms of bacteria-plant interaction may be used in management systems that seek a sustainable production of several crops in agriculture, mainly sugar cane, which has great importance in today's economy. Therefore, the objective was to evaluate the diversity of bacteria (endophytic and rhizoplane) associated with different varieties of cane sugar grown in the Northeast and select bacteria with potential to promote plant growth (nitrogen fixing and phosphate solubilizing) for a possible reduction of fertilizer. Endophytic bacteria, leaf and root, and rhizoplane were isolated from three commercial varieties (RB 92579, RB 867515 and RB 863129) of cane sugar grown in the Northeast, in areas with and without the application of Termiticide and in two seasons (4 and 10 months) of plant development. The bacteria were isolated in 10% TSA media and selective media NFB and selected based on their ability to solubilize inorganic phosphate and nitrogen fixation. At the end of the process were isolated about 410 strains in rich media (TSA) and about 245 isolated media NFB(diazotrophic), that for both harvests. In the interaction bacteria-cane sugar, the total population of yellow and white bacteria was influenced by the varieties of cane and evaluated by plant tissues, and its management, and, moreover, it was concluded that the varieties of sugarcane sugarcane grown in the Northeast have a bacterial community associated with potential application to plant growth promotion, since they have the ability to fix nitrogen, solubilize inorganic phosphate and produce AIA. Plant cane sugar grown in Pernambuco has a distinct community of endophytic bacteria in relation to other producing states, some being identified as the genus Burkholderia, Pantoea, Klebsiella, Pseudomonas and Enterobacter.
id URPE_54502b490b90f580d95a629d655e1932
oai_identifier_str oai:tede2:tede2/5273
network_acronym_str URPE
network_name_str Biblioteca Digital de Teses e Dissertações da UFRPE
repository_id_str
spelling FREIRE, Fernando JoséSILVA, Gláucía Alves eSILVA, Maria Luiza Ribeiro Bastos daLIRA JÚNIOR, Mário de AndradeSTAMFORD, Newton Pereirahttp://lattes.cnpq.br/5831953262176334SILVA, Michelangelo de Oliveira2016-08-11T12:22:48Z2011-02-24SILVA, Michelangelo de Oliveira. Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal. 2011. 70 f. Tese (Programa de Pós-Graduação em Ciência do Solo) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5273The exploration and understanding of the mechanisms of bacteria-plant interaction may be used in management systems that seek a sustainable production of several crops in agriculture, mainly sugar cane, which has great importance in today's economy. Therefore, the objective was to evaluate the diversity of bacteria (endophytic and rhizoplane) associated with different varieties of cane sugar grown in the Northeast and select bacteria with potential to promote plant growth (nitrogen fixing and phosphate solubilizing) for a possible reduction of fertilizer. Endophytic bacteria, leaf and root, and rhizoplane were isolated from three commercial varieties (RB 92579, RB 867515 and RB 863129) of cane sugar grown in the Northeast, in areas with and without the application of Termiticide and in two seasons (4 and 10 months) of plant development. The bacteria were isolated in 10% TSA media and selective media NFB and selected based on their ability to solubilize inorganic phosphate and nitrogen fixation. At the end of the process were isolated about 410 strains in rich media (TSA) and about 245 isolated media NFB(diazotrophic), that for both harvests. In the interaction bacteria-cane sugar, the total population of yellow and white bacteria was influenced by the varieties of cane and evaluated by plant tissues, and its management, and, moreover, it was concluded that the varieties of sugarcane sugarcane grown in the Northeast have a bacterial community associated with potential application to plant growth promotion, since they have the ability to fix nitrogen, solubilize inorganic phosphate and produce AIA. Plant cane sugar grown in Pernambuco has a distinct community of endophytic bacteria in relation to other producing states, some being identified as the genus Burkholderia, Pantoea, Klebsiella, Pseudomonas and Enterobacter.A exploração e o conhecimento dos mecanismos de interação bactéria-planta poderão ser utilizados em sistemas de manejo que busquem uma produção sustentável de diversas culturas de interesse agrícola, principalmente a cana-de-açúcar, que exerce grande importância na economia atual. Portanto, o objetivo deste trabalho foi avaliar a diversidade de bactérias (endofíticas e do rizoplano) associadas a diferentes variedades de cana-de-açúcar, cultivadas no Nordeste e selecionar bactérias com potencial em promover o crescimento vegetal (fixadoras de nitrogênio e solubilizadoras de fosfato), para uma possível redução de fertilizantes. Bactérias endofíticas, de folha e raiz, e do rizoplano foram isoladas de três variedades comerciais (RB 92579, RB 867515 e RB 863129) de cana-de-açúcar, cultivadas no Nordeste, em áreas com e sem aplicação de cupinicida e em duas épocas (4 e 10 meses) de desenvolvimento da planta . As bactérias foram isoladas em meio TSA 10% e no meio seletivo NFb e selecionadas quanto a sua capacidade de solubilizar fosfato inorgânico e fixar nitrogênio. No final do processo foram isoladas cerca de 410 isolados em meio rico (TSA) e cerca de 245 isolados em meio NFb (diazotroficas), isso para ambas épocas de coleta. Na interação bactéria-cana-de-açúcar, a densidade populacional total, de bactérias amarelas e brancas foi influenciada pelas variedades de cana avaliadas e pelos tecidos vegetais, e pelo manejo e, além disso, foi possível concluir que as variedades de cana-de-açúcar cultivadas no Nordeste apresentam uma comunidade bacteriana associada com potencial aplicação para a promoção de crescimento vegetal, pois possuem a capacidade de fixar nitrogênio, solubilizar fosfato inorgânico e produzir AIA. Planta de cana-de-açúcar cultivada em Pernambuco possui uma comunidade de bactérias endofíticas distinta em relação aos outros estados produtores, sendo algumas identificadas como dos gêneros: Burkholderia, Pantoea, Klebsiella, Pseudomonas e Enterobacter.Submitted by (lucia.rodrigues@ufrpe.br) on 2016-08-11T12:22:48Z No. of bitstreams: 1 Michelangelo de Oliveira Silva.pdf: 667109 bytes, checksum: d1d78400e5eb31539cdab86c7d14b716 (MD5)Made available in DSpace on 2016-08-11T12:22:48Z (GMT). No. of bitstreams: 1 Michelangelo de Oliveira Silva.pdf: 667109 bytes, checksum: d1d78400e5eb31539cdab86c7d14b716 (MD5) Previous issue date: 2011-02-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência do SoloUFRPEBrasilDepartamento de AgronomiaCana-de-açúcarTecnologia agrícolaSolubilização de fosfatoFixação de nitrogênioDiazotroficasEcologia microbianaMicrobial EcologyNitrogen FixationPhosphate SolubilizationDiazotrophicAGRONOMIA::CIENCIA DO SOLOBactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.Bacteria associated with sugar cane: isolation and potential plant growth promotion.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8551734419043507650600600600600600-6800553879972229205-59198405272323756712075167498588264571-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5273/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALMichelangelo de Oliveira Silva.pdfMichelangelo de Oliveira Silva.pdfapplication/pdf667109http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5273/2/Michelangelo+de+Oliveira+Silva.pdfd1d78400e5eb31539cdab86c7d14b716MD52tede2/52732016-08-11 09:22:48.716oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2016-08-11T12:22:48Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
dc.title.alternative.eng.fl_str_mv Bacteria associated with sugar cane: isolation and potential plant growth promotion.
title Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
spellingShingle Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
SILVA, Michelangelo de Oliveira
Cana-de-açúcar
Tecnologia agrícola
Solubilização de fosfato
Fixação de nitrogênio
Diazotroficas
Ecologia microbiana
Microbial Ecology
Nitrogen Fixation
Phosphate Solubilization
Diazotrophic
AGRONOMIA::CIENCIA DO SOLO
title_short Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
title_full Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
title_fullStr Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
title_full_unstemmed Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
title_sort Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal.
author SILVA, Michelangelo de Oliveira
author_facet SILVA, Michelangelo de Oliveira
author_role author
dc.contributor.advisor1.fl_str_mv FREIRE, Fernando José
dc.contributor.referee1.fl_str_mv SILVA, Gláucía Alves e
dc.contributor.referee2.fl_str_mv SILVA, Maria Luiza Ribeiro Bastos da
dc.contributor.referee3.fl_str_mv LIRA JÚNIOR, Mário de Andrade
dc.contributor.referee4.fl_str_mv STAMFORD, Newton Pereira
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5831953262176334
dc.contributor.author.fl_str_mv SILVA, Michelangelo de Oliveira
contributor_str_mv FREIRE, Fernando José
SILVA, Gláucía Alves e
SILVA, Maria Luiza Ribeiro Bastos da
LIRA JÚNIOR, Mário de Andrade
STAMFORD, Newton Pereira
dc.subject.por.fl_str_mv Cana-de-açúcar
Tecnologia agrícola
Solubilização de fosfato
Fixação de nitrogênio
Diazotroficas
Ecologia microbiana
Microbial Ecology
Nitrogen Fixation
Phosphate Solubilization
Diazotrophic
topic Cana-de-açúcar
Tecnologia agrícola
Solubilização de fosfato
Fixação de nitrogênio
Diazotroficas
Ecologia microbiana
Microbial Ecology
Nitrogen Fixation
Phosphate Solubilization
Diazotrophic
AGRONOMIA::CIENCIA DO SOLO
dc.subject.cnpq.fl_str_mv AGRONOMIA::CIENCIA DO SOLO
description The exploration and understanding of the mechanisms of bacteria-plant interaction may be used in management systems that seek a sustainable production of several crops in agriculture, mainly sugar cane, which has great importance in today's economy. Therefore, the objective was to evaluate the diversity of bacteria (endophytic and rhizoplane) associated with different varieties of cane sugar grown in the Northeast and select bacteria with potential to promote plant growth (nitrogen fixing and phosphate solubilizing) for a possible reduction of fertilizer. Endophytic bacteria, leaf and root, and rhizoplane were isolated from three commercial varieties (RB 92579, RB 867515 and RB 863129) of cane sugar grown in the Northeast, in areas with and without the application of Termiticide and in two seasons (4 and 10 months) of plant development. The bacteria were isolated in 10% TSA media and selective media NFB and selected based on their ability to solubilize inorganic phosphate and nitrogen fixation. At the end of the process were isolated about 410 strains in rich media (TSA) and about 245 isolated media NFB(diazotrophic), that for both harvests. In the interaction bacteria-cane sugar, the total population of yellow and white bacteria was influenced by the varieties of cane and evaluated by plant tissues, and its management, and, moreover, it was concluded that the varieties of sugarcane sugarcane grown in the Northeast have a bacterial community associated with potential application to plant growth promotion, since they have the ability to fix nitrogen, solubilize inorganic phosphate and produce AIA. Plant cane sugar grown in Pernambuco has a distinct community of endophytic bacteria in relation to other producing states, some being identified as the genus Burkholderia, Pantoea, Klebsiella, Pseudomonas and Enterobacter.
publishDate 2011
dc.date.issued.fl_str_mv 2011-02-24
dc.date.accessioned.fl_str_mv 2016-08-11T12:22:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Michelangelo de Oliveira. Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal. 2011. 70 f. Tese (Programa de Pós-Graduação em Ciência do Solo) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5273
identifier_str_mv SILVA, Michelangelo de Oliveira. Bactérias associadas à cana-de-açúcar: isolamento e potencial promoção de crescimento vegetal. 2011. 70 f. Tese (Programa de Pós-Graduação em Ciência do Solo) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5273
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 8551734419043507650
dc.relation.confidence.fl_str_mv 600
600
600
600
600
dc.relation.department.fl_str_mv -6800553879972229205
dc.relation.cnpq.fl_str_mv -5919840527232375671
dc.relation.sponsorship.fl_str_mv 2075167498588264571
-2555911436985713659
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência do Solo
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Agronomia
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRPE
instname:Universidade Federal Rural de Pernambuco (UFRPE)
instacron:UFRPE
instname_str Universidade Federal Rural de Pernambuco (UFRPE)
instacron_str UFRPE
institution UFRPE
reponame_str Biblioteca Digital de Teses e Dissertações da UFRPE
collection Biblioteca Digital de Teses e Dissertações da UFRPE
bitstream.url.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5273/1/license.txt
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5273/2/Michelangelo+de+Oliveira+Silva.pdf
bitstream.checksum.fl_str_mv bd3efa91386c1718a7f26a329fdcb468
d1d78400e5eb31539cdab86c7d14b716
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)
repository.mail.fl_str_mv bdtd@ufrpe.br ||bdtd@ufrpe.br
_version_ 1791083570853314560