Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5818 |
Resumo: | Visceral Leishmaniasis (VL) is a zoonosis transmitted by sand flies caused by several Leishmania species and that can infect humans and domestic and wild animals. In Brazil, the growth in the number of cases of Canine Visceral Leishmaniasis (CVL) is associated with the adaptation of its vector and dispersion of Leishmania infantum among domestic and wild hosts in urban and periurban areas. The objective of this work contribute to the epidemiological study of CVL in the state of Pernambuco by identification of the frequency IgG antibodies anti-Leishmania infantum in free-living and captives crab-eating foxes (Cerdocyon thous) and domestic dogs (Canis familiaris) from Conservation Unit of the State of Pernambuco and its surroundings. Blood samples were obtained of 107 animals, 18 crabeating foxes (four free-living and 14 captives) and of 89 dogs (85 domiciled and four strays). Bone marrow samples were obtained of nine crab-eating foxes and three dogs. The IFAT and ELISA were performed on blood samples of 107 dogs and PCR was performed on 12 samples of bone marrow. Of the 18 crab-eating foxes examined, one (5.5%) were seropositive to the ELISA and 89 dogs examined, 29.21% were positive by IFAT, 35.95% positive by ELISA and 19.10% were positive in both tests simultaneously. None of the samples was positive by PCR. It is concluded that the presence of IgG antibodies anti-L. infantum in a free-living crabeating fox and in a stray dog in the wild environment suggested the existence of a local sylvatic cycle of Leishmania infantum in one of the areas of study. The presence of seropositive dogs inside and around of these wild areas indicated that animals can act as dispersers of the infectious agent to humans and to the non-infected dogs. This is the first occurrence of IgG antibodies to Leishmania infantum in free-living crab-eating fox and dogs trapped inside of the Conservation Units of the State of Pernambuco, Brazil. |
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SILVA, Jean Carlos Ramos daALVES, Leucio CâmaraMOTA, Rinaldo AparecidoMARVULO, Maria Fernanda ViannaSILVA, José Sérgio de Alcantara ehttp://lattes.cnpq.br/0237426891229146OLIVEIRA, Rafael Lima de2016-10-21T14:47:00Z2012-02-23OLIVEIRA, Rafael Lima de. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. 2012. 53 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5818Visceral Leishmaniasis (VL) is a zoonosis transmitted by sand flies caused by several Leishmania species and that can infect humans and domestic and wild animals. In Brazil, the growth in the number of cases of Canine Visceral Leishmaniasis (CVL) is associated with the adaptation of its vector and dispersion of Leishmania infantum among domestic and wild hosts in urban and periurban areas. The objective of this work contribute to the epidemiological study of CVL in the state of Pernambuco by identification of the frequency IgG antibodies anti-Leishmania infantum in free-living and captives crab-eating foxes (Cerdocyon thous) and domestic dogs (Canis familiaris) from Conservation Unit of the State of Pernambuco and its surroundings. Blood samples were obtained of 107 animals, 18 crabeating foxes (four free-living and 14 captives) and of 89 dogs (85 domiciled and four strays). Bone marrow samples were obtained of nine crab-eating foxes and three dogs. The IFAT and ELISA were performed on blood samples of 107 dogs and PCR was performed on 12 samples of bone marrow. Of the 18 crab-eating foxes examined, one (5.5%) were seropositive to the ELISA and 89 dogs examined, 29.21% were positive by IFAT, 35.95% positive by ELISA and 19.10% were positive in both tests simultaneously. None of the samples was positive by PCR. It is concluded that the presence of IgG antibodies anti-L. infantum in a free-living crabeating fox and in a stray dog in the wild environment suggested the existence of a local sylvatic cycle of Leishmania infantum in one of the areas of study. The presence of seropositive dogs inside and around of these wild areas indicated that animals can act as dispersers of the infectious agent to humans and to the non-infected dogs. This is the first occurrence of IgG antibodies to Leishmania infantum in free-living crab-eating fox and dogs trapped inside of the Conservation Units of the State of Pernambuco, Brazil.A Leishmaniose Visceral (LV) é uma zoonose transmitida por flebotomíneos, sendo causada por diversas espécies de Leishmania e que podem infectar seres humanos, e animais domésticos e selvagens. No Brasil, o crescimento no número de casos de Leishmaniose Visceral Canina (LVC) é associado com a adaptação de seu vetor e a dispersão da Leishmania infantum entre os hospedeiros domésticos e silvestres no ambiente urbano e periurbano. Objetivou-se com este trabalho contribuir com o estudo epidemiológico da LVC no Estado de Pernambuco, mediante a identificação da frequência anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) oriundos de Unidades de Conservação do Estado de Pernambuco e no seu entorno. Ao todo, foram obtidas amostras de sangue de 107 animais, sendo 18 raposas (quatro de vida livre e 14 de cativeiro) e de 89 cães (85 domiciliados e quatro errantes). Em nove raposas e três cães também foram obtidas amostras de medula óssea. Os testes de RIFI e ELISA foram realizados nas 107 amostras de sangue dos canídeos e a PCR foi realizada nas 12 amostras de medula óssea. Das 18 raposas examinadas, uma (5,5%) foi soropositiva ao ELISA e dos 89 cães examinados, 29,21% foram soropositivos pela RIFI, 35,95% pelo ELISA e 19,10% foram soropositivos em ambos os testes simultaneamente. Nenhuma das amostras foi positiva na PCR. Concluiu-se que a ocorrência de anticorpos IgG anti-L. infantum em uma raposa de vida livre e um cão errante no mesmo ambiente silvestre sugeriu a existência de um ciclo silvático da Leishmania infantum em uma das áreas de estudo. A presença de cães soropositivos no interior e no entorno de áreas silvestres indicou que estes animais podem atuar como dispersores do agente infeccioso para populações humanas e para cães não infectados. Esta é a primeira ocorrência de anticorpos IgG anti-Leishmania infantum em raposa de vida livre e em cães capturados no interior de Unidades Conservação do Estado de Pernambuco, Brasil.Submitted by (edna.saturno@ufrpe.br) on 2016-10-21T14:47:00Z No. of bitstreams: 1 Rafael Lima de Oliveira.pdf: 312568 bytes, checksum: 45b29c2e75fa734e4b695db3a93916d9 (MD5)Made available in DSpace on 2016-10-21T14:47:00Z (GMT). No. of bitstreams: 1 Rafael Lima de Oliveira.pdf: 312568 bytes, checksum: 45b29c2e75fa734e4b695db3a93916d9 (MD5) Previous issue date: 2012-02-23application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência VeterináriaUFRPEBrasilDepartamento de Medicina VeterináriaLeishmaniaRaposaCãoAnticorposEpidemiologiaCIENCIAS AGRARIAS::MEDICINA VETERINARIAPesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambucoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3061482854177903105600600600-3020210563763616780453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5818/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALRafael Lima de Oliveira.pdfRafael Lima de Oliveira.pdfapplication/pdf312568http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5818/2/Rafael+Lima+de+Oliveira.pdf45b29c2e75fa734e4b695db3a93916d9MD52tede2/58182018-06-14 10:47:04.657oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:33:34.464550Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
title |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
spellingShingle |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco OLIVEIRA, Rafael Lima de Leishmania Raposa Cão Anticorpos Epidemiologia CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
title_full |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
title_fullStr |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
title_full_unstemmed |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
title_sort |
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco |
author |
OLIVEIRA, Rafael Lima de |
author_facet |
OLIVEIRA, Rafael Lima de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
SILVA, Jean Carlos Ramos da |
dc.contributor.advisor-co1.fl_str_mv |
ALVES, Leucio Câmara |
dc.contributor.referee1.fl_str_mv |
MOTA, Rinaldo Aparecido |
dc.contributor.referee2.fl_str_mv |
MARVULO, Maria Fernanda Vianna |
dc.contributor.referee3.fl_str_mv |
SILVA, José Sérgio de Alcantara e |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0237426891229146 |
dc.contributor.author.fl_str_mv |
OLIVEIRA, Rafael Lima de |
contributor_str_mv |
SILVA, Jean Carlos Ramos da ALVES, Leucio Câmara MOTA, Rinaldo Aparecido MARVULO, Maria Fernanda Vianna SILVA, José Sérgio de Alcantara e |
dc.subject.por.fl_str_mv |
Leishmania Raposa Cão Anticorpos Epidemiologia |
topic |
Leishmania Raposa Cão Anticorpos Epidemiologia CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Visceral Leishmaniasis (VL) is a zoonosis transmitted by sand flies caused by several Leishmania species and that can infect humans and domestic and wild animals. In Brazil, the growth in the number of cases of Canine Visceral Leishmaniasis (CVL) is associated with the adaptation of its vector and dispersion of Leishmania infantum among domestic and wild hosts in urban and periurban areas. The objective of this work contribute to the epidemiological study of CVL in the state of Pernambuco by identification of the frequency IgG antibodies anti-Leishmania infantum in free-living and captives crab-eating foxes (Cerdocyon thous) and domestic dogs (Canis familiaris) from Conservation Unit of the State of Pernambuco and its surroundings. Blood samples were obtained of 107 animals, 18 crabeating foxes (four free-living and 14 captives) and of 89 dogs (85 domiciled and four strays). Bone marrow samples were obtained of nine crab-eating foxes and three dogs. The IFAT and ELISA were performed on blood samples of 107 dogs and PCR was performed on 12 samples of bone marrow. Of the 18 crab-eating foxes examined, one (5.5%) were seropositive to the ELISA and 89 dogs examined, 29.21% were positive by IFAT, 35.95% positive by ELISA and 19.10% were positive in both tests simultaneously. None of the samples was positive by PCR. It is concluded that the presence of IgG antibodies anti-L. infantum in a free-living crabeating fox and in a stray dog in the wild environment suggested the existence of a local sylvatic cycle of Leishmania infantum in one of the areas of study. The presence of seropositive dogs inside and around of these wild areas indicated that animals can act as dispersers of the infectious agent to humans and to the non-infected dogs. This is the first occurrence of IgG antibodies to Leishmania infantum in free-living crab-eating fox and dogs trapped inside of the Conservation Units of the State of Pernambuco, Brazil. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-23 |
dc.date.accessioned.fl_str_mv |
2016-10-21T14:47:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Rafael Lima de. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. 2012. 53 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5818 |
identifier_str_mv |
OLIVEIRA, Rafael Lima de. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. 2012. 53 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5818 |
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por |
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Universidade Federal Rural de Pernambuco |
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