Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7837 |
Resumo: | The occurrence of foot rot caused by Diaporthe destruens compromises the productivity of sweet potato and poses a threat to the entire production chain of tuber, there are no fungicide registered in the Ministério da Agricultura, Pecuária e Abastecimento (MAPA) for the management of this disease. Biological control, using Trichoderma spp. and yeast, promising antagonist agents, is an efficient solution and can be applied with the crop management. The study aimed to select species of Trichoderma and yeasts with biocontrol potential on Diaporthe destruens. The pathogenicity test was performed with the isolates obtained from diseased branches, selecting the most aggressive for the studies. In vitro antagonism of Trichoderma spp. was evaluated by pairing culture and yeast by pairing culture, compound diffusion and Killer toxin production. In the in vivo tests, sweet potato seedlings, Co-Roxa and Co-Branca varieties, were produced in a greenhouse. For the trials with Trichoderma spp. the treatments were composed of substrate infested with the nine isolates of the antagonist. For the yeast tests, the plants were sprayed by a cell suspension of the selected isolates in vitro. In both testes, the inoculation of the pathogen was carried out through disks, in two distinct regions of the plant. 110 fungal isolates were obtained, in which 60 were pathogenic, selecting the isolate TH9 (Diaporthe destruens) for the tests. Of the 21 isolates of Trichoderma spp. obtained in the study, nine obtained better results in the pairing and three were more efficient in controlling the pathogen in vivo, identifying two species T. longigbrachiatum (013) and T. asperellum (162 and 177). Of the 52 yeasts tested, 15 showed 100% inhibition of the mycelial growth of the pathogen in the diffusion of compounds. In the Killer toxin assays, there was a difference in halo intensity expressed by yeasts. In both varieties, four isolates were efficient in reducing the symptoms of the disease in vivo, identifying two species of yeasts Moesziomyces aphidis (LFB13 and LCB15) and Candida orthopsilosis (LFB22 and LFB25) potentially antagonists. This is the first report of the reduction of the symptoms of foot rot of sweet potato by the antagonistic action of Trichoderma spp., M. aphidis and C. orthopsilosis. These antagonists can be used in the management of the disease. |
id |
URPE_66efdf65431a60ecd4db09798d0a0d38 |
---|---|
oai_identifier_str |
oai:tede2:tede2/7837 |
network_acronym_str |
URPE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
repository_id_str |
|
spelling |
LARANJEIRA, DelsonCÂMARA, Marcos Paz SaraivaNEVES, Rejane PereiraLARANJEIRA, DelsonNEVES, Rejane PereiraSOUZA, Elineide Barbosa dehttp://lattes.cnpq.br/3780417002982799ALMEIDA, Thaís Regina Pintino de2019-02-14T12:56:26Z2018-02-27ALMEIDA, Thaís Regina Pintino de. Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens. 2018. 74 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7837The occurrence of foot rot caused by Diaporthe destruens compromises the productivity of sweet potato and poses a threat to the entire production chain of tuber, there are no fungicide registered in the Ministério da Agricultura, Pecuária e Abastecimento (MAPA) for the management of this disease. Biological control, using Trichoderma spp. and yeast, promising antagonist agents, is an efficient solution and can be applied with the crop management. The study aimed to select species of Trichoderma and yeasts with biocontrol potential on Diaporthe destruens. The pathogenicity test was performed with the isolates obtained from diseased branches, selecting the most aggressive for the studies. In vitro antagonism of Trichoderma spp. was evaluated by pairing culture and yeast by pairing culture, compound diffusion and Killer toxin production. In the in vivo tests, sweet potato seedlings, Co-Roxa and Co-Branca varieties, were produced in a greenhouse. For the trials with Trichoderma spp. the treatments were composed of substrate infested with the nine isolates of the antagonist. For the yeast tests, the plants were sprayed by a cell suspension of the selected isolates in vitro. In both testes, the inoculation of the pathogen was carried out through disks, in two distinct regions of the plant. 110 fungal isolates were obtained, in which 60 were pathogenic, selecting the isolate TH9 (Diaporthe destruens) for the tests. Of the 21 isolates of Trichoderma spp. obtained in the study, nine obtained better results in the pairing and three were more efficient in controlling the pathogen in vivo, identifying two species T. longigbrachiatum (013) and T. asperellum (162 and 177). Of the 52 yeasts tested, 15 showed 100% inhibition of the mycelial growth of the pathogen in the diffusion of compounds. In the Killer toxin assays, there was a difference in halo intensity expressed by yeasts. In both varieties, four isolates were efficient in reducing the symptoms of the disease in vivo, identifying two species of yeasts Moesziomyces aphidis (LFB13 and LCB15) and Candida orthopsilosis (LFB22 and LFB25) potentially antagonists. This is the first report of the reduction of the symptoms of foot rot of sweet potato by the antagonistic action of Trichoderma spp., M. aphidis and C. orthopsilosis. These antagonists can be used in the management of the disease.A ocorrência do Mal-do-pé da batata-doce, que tem como agente causal Diaporthe destruens, representa uma ameaça para toda a cadeia de produtiva da cultura, devido à dificuldade de manejo da doença, não havendo registro de fungicidas no Ministério da Agricultura Pecuária e Abastecimento (MAPA) para manejo da doença. O controle biológico, utilizando Trichoderma spp. e leveduras, promissores agentes antagonistas, é uma solução eficiente e pode ser aplicada com o manejo da cultura. O trabalho teve como objetivo selecionar espécies de Trichoderma e de leveduras com potencial biocontrolador sobre Diaporthe destruens. Realizou-se o teste de patogenicidade com os isolados obtidos de ramas doentes, selecionando o mais agressivo para os estudos. Avaliou-se o antagonismo in vitro dos isolados de Trichoderma spp., através do pareamento de cultura e das leveduras por cultivo conjunto, difusão de compostos e produção de toxinas Killer. Para realização dos testes in vivo, as mudas de batata-doce, varieades Co-Roxa e Co-Branca, foram produzidas em casa de vegetação. Para os ensaios com Trichoderma spp., os tratamentos foram compostos de substrato infestado com os nove isolados do antagonista. Para os testes com as leveduras, as plantas foram aspergidas por uma suspensão de células dos 15 isolados selecionados in vitro. Em ambos os ensaios, a inoculação do patógeno foi realizada através de discos, em duas regiões distintas da planta. Foram obtidos 110 isolados fúngicos, nos quais 60 foram patogênicos, selecionando o isolado TH9 (Diaporthe destruens) para os ensaios. Dos 21 isolados de Trichoderma spp. obtidos para o estudo, nove obtiveram melhores resultados no pareamento e três foram mais eficientes no controle do patógeno in vivo, identtificando-se duas espécies T. longigbrachiatum (013) e T. asperellum (162 e 177). Das 52 leveduras testadas, 15 apresentaram 100% de inibição do crescimento micelial do patógeno na difusão de compostos. Nos ensaios de toxina Killer, houve diferença de intensidade do halo expressado pelas leveduras. Em ambas as variedades, quatro isolados foram eficientes na redução dos sintomas da doença in vivo, identificando-se duas espécies de leveduras Moesziomyces aphidis (LFB13 e LCB15) e Candida orthopsilosis (LFB22 e LFB25) potencialmente biocontroladoras. Este é o primeiro relato da redução dos sintomas do mal-do-pé da batata-doce pela ação antagônica de Trichoderma spp., M. aphidis e C. orthopsilosis, podendo esses antagonistas serem utilizados no manejo da doença.Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-02-14T12:56:26Z No. of bitstreams: 1 Thais Regina Pintino de Almeida.pdf: 833198 bytes, checksum: ea621e05b8e081795219bd7687bf1887 (MD5)Made available in DSpace on 2019-02-14T12:56:26Z (GMT). No. of bitstreams: 1 Thais Regina Pintino de Almeida.pdf: 833198 bytes, checksum: ea621e05b8e081795219bd7687bf1887 (MD5) Previous issue date: 2018-02-27Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em FitopatologiaUFRPEBrasilDepartamento de AgronomiaDiaporthe destruensTrichodermaControle biológicoFITOSSANIDADE::FITOPATOLOGIAAtividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1343367238723626701600600600600-6800553879972229205-6207026424523013504-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALThais Regina Pintino de Almeida.pdfThais Regina Pintino de Almeida.pdfapplication/pdf833198http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7837/2/Thais+Regina+Pintino+de+Almeida.pdfea621e05b8e081795219bd7687bf1887MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7837/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/78372019-02-14 09:56:26.164oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:36:11.173316Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
title |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
spellingShingle |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens ALMEIDA, Thaís Regina Pintino de Diaporthe destruens Trichoderma Controle biológico FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
title_full |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
title_fullStr |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
title_full_unstemmed |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
title_sort |
Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens |
author |
ALMEIDA, Thaís Regina Pintino de |
author_facet |
ALMEIDA, Thaís Regina Pintino de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
LARANJEIRA, Delson |
dc.contributor.advisor-co1.fl_str_mv |
CÂMARA, Marcos Paz Saraiva |
dc.contributor.advisor-co2.fl_str_mv |
NEVES, Rejane Pereira |
dc.contributor.referee1.fl_str_mv |
LARANJEIRA, Delson |
dc.contributor.referee2.fl_str_mv |
NEVES, Rejane Pereira |
dc.contributor.referee3.fl_str_mv |
SOUZA, Elineide Barbosa de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3780417002982799 |
dc.contributor.author.fl_str_mv |
ALMEIDA, Thaís Regina Pintino de |
contributor_str_mv |
LARANJEIRA, Delson CÂMARA, Marcos Paz Saraiva NEVES, Rejane Pereira LARANJEIRA, Delson NEVES, Rejane Pereira SOUZA, Elineide Barbosa de |
dc.subject.por.fl_str_mv |
Diaporthe destruens Trichoderma Controle biológico |
topic |
Diaporthe destruens Trichoderma Controle biológico FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.cnpq.fl_str_mv |
FITOSSANIDADE::FITOPATOLOGIA |
description |
The occurrence of foot rot caused by Diaporthe destruens compromises the productivity of sweet potato and poses a threat to the entire production chain of tuber, there are no fungicide registered in the Ministério da Agricultura, Pecuária e Abastecimento (MAPA) for the management of this disease. Biological control, using Trichoderma spp. and yeast, promising antagonist agents, is an efficient solution and can be applied with the crop management. The study aimed to select species of Trichoderma and yeasts with biocontrol potential on Diaporthe destruens. The pathogenicity test was performed with the isolates obtained from diseased branches, selecting the most aggressive for the studies. In vitro antagonism of Trichoderma spp. was evaluated by pairing culture and yeast by pairing culture, compound diffusion and Killer toxin production. In the in vivo tests, sweet potato seedlings, Co-Roxa and Co-Branca varieties, were produced in a greenhouse. For the trials with Trichoderma spp. the treatments were composed of substrate infested with the nine isolates of the antagonist. For the yeast tests, the plants were sprayed by a cell suspension of the selected isolates in vitro. In both testes, the inoculation of the pathogen was carried out through disks, in two distinct regions of the plant. 110 fungal isolates were obtained, in which 60 were pathogenic, selecting the isolate TH9 (Diaporthe destruens) for the tests. Of the 21 isolates of Trichoderma spp. obtained in the study, nine obtained better results in the pairing and three were more efficient in controlling the pathogen in vivo, identifying two species T. longigbrachiatum (013) and T. asperellum (162 and 177). Of the 52 yeasts tested, 15 showed 100% inhibition of the mycelial growth of the pathogen in the diffusion of compounds. In the Killer toxin assays, there was a difference in halo intensity expressed by yeasts. In both varieties, four isolates were efficient in reducing the symptoms of the disease in vivo, identifying two species of yeasts Moesziomyces aphidis (LFB13 and LCB15) and Candida orthopsilosis (LFB22 and LFB25) potentially antagonists. This is the first report of the reduction of the symptoms of foot rot of sweet potato by the antagonistic action of Trichoderma spp., M. aphidis and C. orthopsilosis. These antagonists can be used in the management of the disease. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-02-27 |
dc.date.accessioned.fl_str_mv |
2019-02-14T12:56:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALMEIDA, Thaís Regina Pintino de. Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens. 2018. 74 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7837 |
identifier_str_mv |
ALMEIDA, Thaís Regina Pintino de. Atividade biocontroladora de Trichoderma spp. e de leveduras sobre Diaporthe destruens. 2018. 74 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7837 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
1343367238723626701 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-6800553879972229205 |
dc.relation.cnpq.fl_str_mv |
-6207026424523013504 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Fitopatologia |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Agronomia |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRPE instname:Universidade Federal Rural de Pernambuco (UFRPE) instacron:UFRPE |
instname_str |
Universidade Federal Rural de Pernambuco (UFRPE) |
instacron_str |
UFRPE |
institution |
UFRPE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
collection |
Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7837/2/Thais+Regina+Pintino+de+Almeida.pdf http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7837/1/license.txt |
bitstream.checksum.fl_str_mv |
ea621e05b8e081795219bd7687bf1887 bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
_version_ |
1810102255694643200 |