Potencial antioxidante do mix de farinhas de resíduos da agroindústria

Detalhes bibliográficos
Autor(a) principal: SILVA, Mariana Costa Fonsêca da
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4894
Resumo: Considering the excessive production of agro-industrial waste materials that still holds bioactive compounds with antioxidant properties, this study aimed to evaluate the phenolic content and the antioxidant potential of the mixture of flour of pineapple waste, acerola and guava. Waste, whom a frozen fruit pulp industry, were dried in an oven with air circulation at 40°C. After dehydration were crushed and sieved (16 mesh) to obtain uniform particle size of meals were submitted to analytical determination to quantify the moisture content, protein, lipids, fixed mineral residue (ash), and total carbohydrates. Then, by applying to the experimental design mixtures (simplex centroid), residues combined in different proportions were added to 60% ethanol and water and subjected to shaking (400rpm) at a temperature of 60°C for 30 min to obtain extracts of hydroethanol and watery, respectively, and in response to concentration of phenolic and the scavenging capacity of DPPH radical of the extracts. Defined the proportions of the waste that results in the highest extraction of phenolic compounds, the residues were also subjected to extraction with methanol (60%) and acetone (60%) under the same conditions. In these extracts were quantified total phenolics and the ability to kidnap the DPPH and ABTS radicals. Flours waste presented moisture content <10 g 100g-1, ash content, proteins, lipids and total carbohydrates ranging from 1,71 to 2,47 g 100g-1; 0,78 to 0,95 g 100g-1; From 1,61 to 8,66 g 100g-1 and 100g 83,69 to 89,19 g-1, respectively. The mixture of planning has shown that, regardless of the solvent extractor, the highest amount of phenolic can be extracted to use 100% of acerola residue; 50% acerola residue + 50% of guava residue; 50% acerola residue + 50% pineapple residue, and the mixture of the three residues in equal proportions. The hydroethanol extracts showed DPPH radical scavenging capacity greater than 80% while their aqueous extracts value reached less than 65%. Larger quantities of phenolic these mixtures were extracted using 60% acetone, however, due to the necessity of removal of the solvent, with a view to use it in food, it was observed that the solvent evaporation process caused a significant reduction in the content of the extracted phenolic. The antioxidant capacity of the extracts of these mixtures against the ABTS radical was higher for acetone extract with waste acerola (67,75 μM Trolox/g flour). The aqueous extracts of mixtures of flour waste acerola and guava as well as mixtures of acerola and pineapple showed a strong positive correlation (r=0,9666 and r=0,9632, respectively) between the concentration of phenolic and the ability antioxidant extracts. The results demonstrated the feasibility of using mixtures of waste and 60% ethanol for extraction of phenolic with prospects to apply them in food.
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spelling MELO, Enayde de AlmeidaSANTOS, Andrelina Maria PinheiroANDRADE, Samara Alvachian CardosoLIVERA, Alda Veronica Souzahttp://lattes.cnpq.br/3616971253144550SILVA, Mariana Costa Fonsêca da2016-06-29T14:09:38Z2015-02-27SILVA, Mariana Costa Fonsêca da. Potencial antioxidante do mix de farinhas de resíduos da agroindústria. 2015. 73 f. Dissertação (Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4894Considering the excessive production of agro-industrial waste materials that still holds bioactive compounds with antioxidant properties, this study aimed to evaluate the phenolic content and the antioxidant potential of the mixture of flour of pineapple waste, acerola and guava. Waste, whom a frozen fruit pulp industry, were dried in an oven with air circulation at 40°C. After dehydration were crushed and sieved (16 mesh) to obtain uniform particle size of meals were submitted to analytical determination to quantify the moisture content, protein, lipids, fixed mineral residue (ash), and total carbohydrates. Then, by applying to the experimental design mixtures (simplex centroid), residues combined in different proportions were added to 60% ethanol and water and subjected to shaking (400rpm) at a temperature of 60°C for 30 min to obtain extracts of hydroethanol and watery, respectively, and in response to concentration of phenolic and the scavenging capacity of DPPH radical of the extracts. Defined the proportions of the waste that results in the highest extraction of phenolic compounds, the residues were also subjected to extraction with methanol (60%) and acetone (60%) under the same conditions. In these extracts were quantified total phenolics and the ability to kidnap the DPPH and ABTS radicals. Flours waste presented moisture content <10 g 100g-1, ash content, proteins, lipids and total carbohydrates ranging from 1,71 to 2,47 g 100g-1; 0,78 to 0,95 g 100g-1; From 1,61 to 8,66 g 100g-1 and 100g 83,69 to 89,19 g-1, respectively. The mixture of planning has shown that, regardless of the solvent extractor, the highest amount of phenolic can be extracted to use 100% of acerola residue; 50% acerola residue + 50% of guava residue; 50% acerola residue + 50% pineapple residue, and the mixture of the three residues in equal proportions. The hydroethanol extracts showed DPPH radical scavenging capacity greater than 80% while their aqueous extracts value reached less than 65%. Larger quantities of phenolic these mixtures were extracted using 60% acetone, however, due to the necessity of removal of the solvent, with a view to use it in food, it was observed that the solvent evaporation process caused a significant reduction in the content of the extracted phenolic. The antioxidant capacity of the extracts of these mixtures against the ABTS radical was higher for acetone extract with waste acerola (67,75 μM Trolox/g flour). The aqueous extracts of mixtures of flour waste acerola and guava as well as mixtures of acerola and pineapple showed a strong positive correlation (r=0,9666 and r=0,9632, respectively) between the concentration of phenolic and the ability antioxidant extracts. The results demonstrated the feasibility of using mixtures of waste and 60% ethanol for extraction of phenolic with prospects to apply them in food.Considerando a produção excessiva de resíduos agroindustriais, materiais que ainda detém compostos bioativos com propriedade antioxidante, este trabalho teve como objetivo avaliar o teor de fenólicos e o potencial antioxidante da mistura de farinhas dos resíduos de abacaxi, acerola e goiaba. Os resíduos, cedidos por uma indústria de polpa congelada de frutas, foram secos em estufa com circulação de ar a 40ºC. Após a desidratação foram triturados e peneirados (16 mesh) para a obtenção de farinhas de granulometria uniforme que foram submetidas a determinação analítica para a quantificação do teor de umidade, proteina, lipideos, resíduo mineral fixo (cinzas), e carboidratos totais. Em seguida, aplicando o planejamento experimental para misturas (simplex-centroide), os resíduos combinados em diferentes proporções foram adicionados ao etanol 60% e água e submetidos a agitação (400rpm), em temperatura de 60ºC, por 30 min para a obtenção dos extratos hidroetanólico e aquoso, respectivamente, tendo como resposta a concentração de fenólicos e a capacidade de sequestro do radical DPPH dos extratos obtidos. Definidas as proporções dos resíduos que propiciaram a maior extração de fenólicos totais, os resíduos foram, também, submetidos à extração com metanol (60%) e com acetona (60%), nas mesmas condições. Nesses extratos foram quantificados os fenólicos totais e a capacidade de sequestrar o radical DPPH e radical ABTS. As farinhas dos resíduos apresentaram teor de umidade < 10 g 100g-1, teores de cinzas, proteínas, lipídeos e carboidratos totais que variaram de 1,71 a 2,47 g 100g-1; 0,78 a 0,95 g 100g-1; 1,61 a 8,66 g 100g-1 e 83,69 a 89,19 g 100g-1, respectivamente. O planejamento de mistura demonstrou que, independente do solvente extrator, a maior quantidade de fenólicos pode ser extraída ao empregar 100% do resíduo de acerola; 50% de resíduo de acerola + 50% do resíduo de goiaba; 50% de resíduo de acerola + 50% do resíduo de abacaxi, e a mistura dos três resíduos em proporções iguais. Os extratos hidroetanólico apresentaram capacidade de sequestro do radical DPPH superior a 80% enquanto que os respectivos extratos aquosos alcançaram valor inferior a 65%. Maiores quantidades de fenólicos foram extraídas destas misturas empregando acetona 60%, entretanto, frente à necessidade da retirada do solvente, na perspectiva de emprega-lo em alimentos, observou-se que o processo de evaporação do solvente promoveu redução significativa no teor dos fenólicos extraídos. A capacidade antioxidante dos extratos destas misturas frente ao radical ABTS foi maior para o extrato hidroacetônico do residuo de acerola (67,75 μM Trolox/g de farinha). Os extratos aquosos das misturas de farinhas dos resíduos de acerola e goiaba bem como o das misturas de acerola e abacaxi apresentaram uma correlação forte e positiva (r=0,9666 e r=0,9632, respectivamente) entre a concentração de fenólicos e a capacidade antioxidante dos extratos. Os resultados demonstraram a viabilidade de empregar misturas de residuos e etanol 60% para extração de fenólicos com perspectivas de aplicá-los em alimentos.Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-06-29T14:09:38Z No. of bitstreams: 1 Mariana Costa Fonseca da Silva.pdf: 1291457 bytes, checksum: 9666ca9660d672ef747464c8839fb4e1 (MD5)Made available in DSpace on 2016-06-29T14:09:38Z (GMT). No. of bitstreams: 1 Mariana Costa Fonseca da Silva.pdf: 1291457 bytes, checksum: 9666ca9660d672ef747464c8839fb4e1 (MD5) Previous issue date: 2015-02-27application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosUFRPEBrasilDepartamento de Ciências DomésticasResíduo agroindustrialAtividade antioxidantePolpa de frutaComposto fenólicoAgroindustrial wasteAntioxidant activityFruit pulpPhenolic compoundCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSPotencial antioxidante do mix de farinhas de resíduos da agroindústriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3168359563433608541600600600-395403402001511222138879504442384524info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4894/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALMariana Costa Fonseca da Silva.pdfMariana Costa Fonseca da Silva.pdfapplication/pdf1291457http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4894/2/Mariana+Costa+Fonseca+da+Silva.pdf9666ca9660d672ef747464c8839fb4e1MD52tede2/48942024-02-20 12:40:28.474oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-02-20T15:40:28Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Potencial antioxidante do mix de farinhas de resíduos da agroindústria
title Potencial antioxidante do mix de farinhas de resíduos da agroindústria
spellingShingle Potencial antioxidante do mix de farinhas de resíduos da agroindústria
SILVA, Mariana Costa Fonsêca da
Resíduo agroindustrial
Atividade antioxidante
Polpa de fruta
Composto fenólico
Agroindustrial waste
Antioxidant activity
Fruit pulp
Phenolic compound
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Potencial antioxidante do mix de farinhas de resíduos da agroindústria
title_full Potencial antioxidante do mix de farinhas de resíduos da agroindústria
title_fullStr Potencial antioxidante do mix de farinhas de resíduos da agroindústria
title_full_unstemmed Potencial antioxidante do mix de farinhas de resíduos da agroindústria
title_sort Potencial antioxidante do mix de farinhas de resíduos da agroindústria
author SILVA, Mariana Costa Fonsêca da
author_facet SILVA, Mariana Costa Fonsêca da
author_role author
dc.contributor.advisor1.fl_str_mv MELO, Enayde de Almeida
dc.contributor.advisor-co1.fl_str_mv SANTOS, Andrelina Maria Pinheiro
dc.contributor.referee1.fl_str_mv ANDRADE, Samara Alvachian Cardoso
dc.contributor.referee2.fl_str_mv LIVERA, Alda Veronica Souza
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3616971253144550
dc.contributor.author.fl_str_mv SILVA, Mariana Costa Fonsêca da
contributor_str_mv MELO, Enayde de Almeida
SANTOS, Andrelina Maria Pinheiro
ANDRADE, Samara Alvachian Cardoso
LIVERA, Alda Veronica Souza
dc.subject.por.fl_str_mv Resíduo agroindustrial
Atividade antioxidante
Polpa de fruta
Composto fenólico
topic Resíduo agroindustrial
Atividade antioxidante
Polpa de fruta
Composto fenólico
Agroindustrial waste
Antioxidant activity
Fruit pulp
Phenolic compound
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Agroindustrial waste
Antioxidant activity
Fruit pulp
Phenolic compound
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description Considering the excessive production of agro-industrial waste materials that still holds bioactive compounds with antioxidant properties, this study aimed to evaluate the phenolic content and the antioxidant potential of the mixture of flour of pineapple waste, acerola and guava. Waste, whom a frozen fruit pulp industry, were dried in an oven with air circulation at 40°C. After dehydration were crushed and sieved (16 mesh) to obtain uniform particle size of meals were submitted to analytical determination to quantify the moisture content, protein, lipids, fixed mineral residue (ash), and total carbohydrates. Then, by applying to the experimental design mixtures (simplex centroid), residues combined in different proportions were added to 60% ethanol and water and subjected to shaking (400rpm) at a temperature of 60°C for 30 min to obtain extracts of hydroethanol and watery, respectively, and in response to concentration of phenolic and the scavenging capacity of DPPH radical of the extracts. Defined the proportions of the waste that results in the highest extraction of phenolic compounds, the residues were also subjected to extraction with methanol (60%) and acetone (60%) under the same conditions. In these extracts were quantified total phenolics and the ability to kidnap the DPPH and ABTS radicals. Flours waste presented moisture content <10 g 100g-1, ash content, proteins, lipids and total carbohydrates ranging from 1,71 to 2,47 g 100g-1; 0,78 to 0,95 g 100g-1; From 1,61 to 8,66 g 100g-1 and 100g 83,69 to 89,19 g-1, respectively. The mixture of planning has shown that, regardless of the solvent extractor, the highest amount of phenolic can be extracted to use 100% of acerola residue; 50% acerola residue + 50% of guava residue; 50% acerola residue + 50% pineapple residue, and the mixture of the three residues in equal proportions. The hydroethanol extracts showed DPPH radical scavenging capacity greater than 80% while their aqueous extracts value reached less than 65%. Larger quantities of phenolic these mixtures were extracted using 60% acetone, however, due to the necessity of removal of the solvent, with a view to use it in food, it was observed that the solvent evaporation process caused a significant reduction in the content of the extracted phenolic. The antioxidant capacity of the extracts of these mixtures against the ABTS radical was higher for acetone extract with waste acerola (67,75 μM Trolox/g flour). The aqueous extracts of mixtures of flour waste acerola and guava as well as mixtures of acerola and pineapple showed a strong positive correlation (r=0,9666 and r=0,9632, respectively) between the concentration of phenolic and the ability antioxidant extracts. The results demonstrated the feasibility of using mixtures of waste and 60% ethanol for extraction of phenolic with prospects to apply them in food.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-27
dc.date.accessioned.fl_str_mv 2016-06-29T14:09:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SILVA, Mariana Costa Fonsêca da. Potencial antioxidante do mix de farinhas de resíduos da agroindústria. 2015. 73 f. Dissertação (Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4894
identifier_str_mv SILVA, Mariana Costa Fonsêca da. Potencial antioxidante do mix de farinhas de resíduos da agroindústria. 2015. 73 f. Dissertação (Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4894
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dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Ciências Domésticas
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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