Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico

Detalhes bibliográficos
Autor(a) principal: SILVA, Ruana Carolina Cabral da
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9509
Resumo: New advances in technologies related to omics favored the identification of signaling mechanisms that regulate plant responses to stress. Nodulation and the ability to fix attach in association with bacteria of the genus Rhizobium spp. May determine plants survival in adverse environments. Among the several classes of genes that act under stress, the nodulins deserve mentioning, as host genes expressed during the nodulation process, being also associated with nodule functioning. Recent studies report that Stylosanthes scabra Vogel has been widely used as forage, presenting high tolerance to environments with poor soils and low water availability. The present project aimed to evaluate the response mechanisms used by S. scabra under abiotic stress (watering suppression), specifically the differential gene expression of early nodulins at different moments after the onset of stress. For this purpose, a selection of seed-sequences was performed, corresponding to cured angiosperm nodulin coding genes available in databases and in the literature. Selected nodulin seed sequences were aligned via tBLASTn against S. scabra transcriptome to select candidates for structural characterization and expression validation. To evaluate the structural diversity, phenological trees were generated. In parallel, the in silico expression profile of the early nodulins was evaluated, and primers were designed for validation of their expression. Two groups of early nodulins have been detected: ENOD and NIN, both exhibiting two conserved domains. For both groups it was possible to identify different conserved residues. Prediction of subcellular localization indicated that ENOD transcripts are associated with the cell membrane, whereas most NIN transcripts were addressed to the nucleus. Regarding the expression profile, 33 ENOD transcripts presented statistically supported data, 16 of them induced and 17 repressed. For NIN, of the total of 55 that presented valid expression, 21 were presented as inductees and 34 as repressed. From the data presented here and considering the agronomic importance of S. scabra, the relevance of the study is noteworthy, with modulated candidate transcripts that have potential for validation studies, besides helping to understand the molecular mechanisms associated with nodulation under stress conditions.
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spelling CARVALHO, Reginaldo deBEZERRA NETO, João PacíficoSILVA, Roberta Lane de OliveiraFERREIRA NETO, José Ribamar CostaMELO, Natoniel Franklin dehttp://lattes.cnpq.br/8634356371128001SILVA, Ruana Carolina Cabral da2024-02-19T14:51:03Z2019-07-31SILVA, Ruana Carolina Cabral da. Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico. 2019. 87 f. Dissertação (Programa de Pós-Graduação em Melhoramento Genético de Plantas) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9509New advances in technologies related to omics favored the identification of signaling mechanisms that regulate plant responses to stress. Nodulation and the ability to fix attach in association with bacteria of the genus Rhizobium spp. May determine plants survival in adverse environments. Among the several classes of genes that act under stress, the nodulins deserve mentioning, as host genes expressed during the nodulation process, being also associated with nodule functioning. Recent studies report that Stylosanthes scabra Vogel has been widely used as forage, presenting high tolerance to environments with poor soils and low water availability. The present project aimed to evaluate the response mechanisms used by S. scabra under abiotic stress (watering suppression), specifically the differential gene expression of early nodulins at different moments after the onset of stress. For this purpose, a selection of seed-sequences was performed, corresponding to cured angiosperm nodulin coding genes available in databases and in the literature. Selected nodulin seed sequences were aligned via tBLASTn against S. scabra transcriptome to select candidates for structural characterization and expression validation. To evaluate the structural diversity, phenological trees were generated. In parallel, the in silico expression profile of the early nodulins was evaluated, and primers were designed for validation of their expression. Two groups of early nodulins have been detected: ENOD and NIN, both exhibiting two conserved domains. For both groups it was possible to identify different conserved residues. Prediction of subcellular localization indicated that ENOD transcripts are associated with the cell membrane, whereas most NIN transcripts were addressed to the nucleus. Regarding the expression profile, 33 ENOD transcripts presented statistically supported data, 16 of them induced and 17 repressed. For NIN, of the total of 55 that presented valid expression, 21 were presented as inductees and 34 as repressed. From the data presented here and considering the agronomic importance of S. scabra, the relevance of the study is noteworthy, with modulated candidate transcripts that have potential for validation studies, besides helping to understand the molecular mechanisms associated with nodulation under stress conditions.Novos avanços nas tecnologias relacionadas às “ômicas” vêm favorecendo a identificação de mecanismos de sinalização que regulam as respostas de vegetais aos estresses. A nodulação e a capacidade de fixar nitrogênio em associação com bactérias do gênero Rhizobium spp. podem determinar a sobrevivência de plantas em ambientes adversos. Entre as diversas classes de genes que atuam em situações de estresse, destacam-se as nodulinas, que são genes do hospedeiro expressos durante o processo de nodulação, também associadas ao funcionamento dos nódulos. Estudos recentes relatam que Stylosanthes scabra Vogel tem sido bastante utilizada como forrageira, apresentando elevada tolerância aos ambientes com solos pobres e baixa disponibilidade hídrica. O presente projeto teve como objetivo avaliar os mecanismos de resposta utilizados por S. scabra sob estresse abiótico (supressão de rega), especificamente a expressão gênica diferencial de nodulinas precoces em diferentes momentos após o início do estresse. Para tanto, foi realizada uma seleção de sequências-sonda correspondentes a genes codificadores de nodulinas curadas de angiospermas disponíveis em bancos de dados e na literatura. As sequências candidatas foram alinhadas via tBLASTn contra o transcriptoma de S. scabra visando selecionar candidatos para sua caracterização estrutural e validação da expressão. Para avaliar a diversidade estrutural, foram geradas árvores fenéticas. Paralelamente, o perfil de expressão in silico das nodulinas precoces foi avaliado, desenhando-se primers para validação de sua expressão. Foram detectados dois grupos gênicos de nodulinas precoces ENOD e NIN, ambos apresentando dois domínios conservados. Para ambos os grupos foi possível identificar diferentes resíduos conservados. A predição da localização subcelular indicou que os transcritos do grupo ENOD estão associados à membrana celular, enquanto a maioria dos transcritos NIN foi endereçada ao núcleo. Em relação ao perfil de expressão, 33 transcritos ENOD apresentaram dados estatisticamente suportados, sendo 16 induzidos e 17 reprimidos. Para NIN, do total de 55 que apresentaram expressão válida, 21 apresentaram-se como induzidos e 34 como reprimidos. A partir dos dados aqui apresentados e considerando-se a importância agronômica de S. scabra, nota-se a relevância do estudo, com transcritos-candidatos modulados que têm potencial para estudos de validação, além de auxiliar no entendimento de mecanismos moleculares associados à nodulação sob condições de estresse.Submitted by (ana.araujo@ufrpe.br) on 2024-02-19T14:51:03Z No. of bitstreams: 1 Ruana Carolina Cabral da Silva.pdf: 3444999 bytes, checksum: ad1ffb1be51e5d995524fb229dd76edf (MD5)Made available in DSpace on 2024-02-19T14:51:03Z (GMT). No. of bitstreams: 1 Ruana Carolina Cabral da Silva.pdf: 3444999 bytes, checksum: ad1ffb1be51e5d995524fb229dd76edf (MD5) Previous issue date: 2019-07-31Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Melhoramento Genético de PlantasUFRPEBrasilDepartamento de AgronomiaLeguminosaStylosanthesNodulaçãoDéficit hídricoRegulação de expressão gênicaBioinformáticaFITOTECNIA::MELHORAMENTO VEGETALDiversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abióticoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-6234655866848882505600600600600-680055387997222920526156072994701319672075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALRuana Carolina Cabral da Silva.pdfRuana Carolina Cabral da Silva.pdfapplication/pdf3444999http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9509/2/Ruana+Carolina+Cabral+da+Silva.pdfad1ffb1be51e5d995524fb229dd76edfMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9509/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/95092024-02-19 11:53:34.824oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:38:22.130078Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
title Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
spellingShingle Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
SILVA, Ruana Carolina Cabral da
Leguminosa
Stylosanthes
Nodulação
Déficit hídrico
Regulação de expressão gênica
Bioinformática
FITOTECNIA::MELHORAMENTO VEGETAL
title_short Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
title_full Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
title_fullStr Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
title_full_unstemmed Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
title_sort Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico
author SILVA, Ruana Carolina Cabral da
author_facet SILVA, Ruana Carolina Cabral da
author_role author
dc.contributor.advisor1.fl_str_mv CARVALHO, Reginaldo de
dc.contributor.advisor-co1.fl_str_mv BEZERRA NETO, João Pacífico
dc.contributor.advisor-co2.fl_str_mv SILVA, Roberta Lane de Oliveira
dc.contributor.referee1.fl_str_mv FERREIRA NETO, José Ribamar Costa
dc.contributor.referee2.fl_str_mv MELO, Natoniel Franklin de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8634356371128001
dc.contributor.author.fl_str_mv SILVA, Ruana Carolina Cabral da
contributor_str_mv CARVALHO, Reginaldo de
BEZERRA NETO, João Pacífico
SILVA, Roberta Lane de Oliveira
FERREIRA NETO, José Ribamar Costa
MELO, Natoniel Franklin de
dc.subject.por.fl_str_mv Leguminosa
Stylosanthes
Nodulação
Déficit hídrico
Regulação de expressão gênica
Bioinformática
topic Leguminosa
Stylosanthes
Nodulação
Déficit hídrico
Regulação de expressão gênica
Bioinformática
FITOTECNIA::MELHORAMENTO VEGETAL
dc.subject.cnpq.fl_str_mv FITOTECNIA::MELHORAMENTO VEGETAL
description New advances in technologies related to omics favored the identification of signaling mechanisms that regulate plant responses to stress. Nodulation and the ability to fix attach in association with bacteria of the genus Rhizobium spp. May determine plants survival in adverse environments. Among the several classes of genes that act under stress, the nodulins deserve mentioning, as host genes expressed during the nodulation process, being also associated with nodule functioning. Recent studies report that Stylosanthes scabra Vogel has been widely used as forage, presenting high tolerance to environments with poor soils and low water availability. The present project aimed to evaluate the response mechanisms used by S. scabra under abiotic stress (watering suppression), specifically the differential gene expression of early nodulins at different moments after the onset of stress. For this purpose, a selection of seed-sequences was performed, corresponding to cured angiosperm nodulin coding genes available in databases and in the literature. Selected nodulin seed sequences were aligned via tBLASTn against S. scabra transcriptome to select candidates for structural characterization and expression validation. To evaluate the structural diversity, phenological trees were generated. In parallel, the in silico expression profile of the early nodulins was evaluated, and primers were designed for validation of their expression. Two groups of early nodulins have been detected: ENOD and NIN, both exhibiting two conserved domains. For both groups it was possible to identify different conserved residues. Prediction of subcellular localization indicated that ENOD transcripts are associated with the cell membrane, whereas most NIN transcripts were addressed to the nucleus. Regarding the expression profile, 33 ENOD transcripts presented statistically supported data, 16 of them induced and 17 repressed. For NIN, of the total of 55 that presented valid expression, 21 were presented as inductees and 34 as repressed. From the data presented here and considering the agronomic importance of S. scabra, the relevance of the study is noteworthy, with modulated candidate transcripts that have potential for validation studies, besides helping to understand the molecular mechanisms associated with nodulation under stress conditions.
publishDate 2019
dc.date.issued.fl_str_mv 2019-07-31
dc.date.accessioned.fl_str_mv 2024-02-19T14:51:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SILVA, Ruana Carolina Cabral da. Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico. 2019. 87 f. Dissertação (Programa de Pós-Graduação em Melhoramento Genético de Plantas) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9509
identifier_str_mv SILVA, Ruana Carolina Cabral da. Diversidade e expressão diferencial de nodulinas precoces no transcriptoma de Stylosanthes scabra Vogel submetida a estresse abiótico. 2019. 87 f. Dissertação (Programa de Pós-Graduação em Melhoramento Genético de Plantas) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9509
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language por
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dc.relation.confidence.fl_str_mv 600
600
600
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dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Melhoramento Genético de Plantas
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Agronomia
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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