Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5747 |
Resumo: | Canine Visceral Leishmaniasis (CVL) is a severe systemic zoonosos caused by the obligate intracellular protozoan Leishmania infantum. The dogs are the major reservoir of the disease and may develop since asymptomatic infection to a variety of clinical signs, such as peripheral lymphadenopathy, weight loss, lethargy, ulcerative lesions of the skin and fever. Ocular, orthopedic and neurological disorders can also be seen associated with CVL, such as keratitis, seizures, behavioral changes, joint pain and lameness. This fact includes the disease in the differential diagnosis of ophthalmic disorders, osteoarticular and nervous system, especially in endemic areas to infection. The aim of this study was the diagnosis of canine visceral leishmaniasis using samples of conjunctiva, aqueous humor, synovial fluid, synovial membrane and cerebrospinal fluid (CSF). The ELISA test was applied to samples of CSF, synovial fluid and aqueous humor, and histological and immunohistochemical evaluations were performed in the conjunctiva and in the synovial membrane of these animals. The samples were collected from 20 dogs serologically positive for visceral leishmaniasis. Through the immunoenzymatic assay could detect anti-Leishmania IgG antibodies in a sample of CSF and aqueous humor, and six samples of synovial fluid. Immunohistochemistry showed positive staining of amastigotes of L. infantum five synovial membranes and connective five studied. From the results, we conclude that amastigotes of Leishmania sp. are found parasitizing the synovial membrane and the conjunctiva of dogs serologically positive for the disease, as well as specific IgG antibodies in aqueous humor, CSF and synovial fluid of these animals. |
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FAUSTINO, Maria Aparecida da GloriaALVES, Leucio CâmaraSANTOS, Jamile Prado dosLIRA, Nair Silva Cavalcanti deRAMOS, Carlos Alberto do Nascimentohttp://lattes.cnpq.br/5360635816108486SEVERO, Maíra Santos2016-10-18T15:00:33Z2012-02-13SEVERO, Maíra Santos. Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano. 2012. 80 f.Tese ( Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5747Canine Visceral Leishmaniasis (CVL) is a severe systemic zoonosos caused by the obligate intracellular protozoan Leishmania infantum. The dogs are the major reservoir of the disease and may develop since asymptomatic infection to a variety of clinical signs, such as peripheral lymphadenopathy, weight loss, lethargy, ulcerative lesions of the skin and fever. Ocular, orthopedic and neurological disorders can also be seen associated with CVL, such as keratitis, seizures, behavioral changes, joint pain and lameness. This fact includes the disease in the differential diagnosis of ophthalmic disorders, osteoarticular and nervous system, especially in endemic areas to infection. The aim of this study was the diagnosis of canine visceral leishmaniasis using samples of conjunctiva, aqueous humor, synovial fluid, synovial membrane and cerebrospinal fluid (CSF). The ELISA test was applied to samples of CSF, synovial fluid and aqueous humor, and histological and immunohistochemical evaluations were performed in the conjunctiva and in the synovial membrane of these animals. The samples were collected from 20 dogs serologically positive for visceral leishmaniasis. Through the immunoenzymatic assay could detect anti-Leishmania IgG antibodies in a sample of CSF and aqueous humor, and six samples of synovial fluid. Immunohistochemistry showed positive staining of amastigotes of L. infantum five synovial membranes and connective five studied. From the results, we conclude that amastigotes of Leishmania sp. are found parasitizing the synovial membrane and the conjunctiva of dogs serologically positive for the disease, as well as specific IgG antibodies in aqueous humor, CSF and synovial fluid of these animals.A Leishmaniose Visceral Canina (LVC) é uma zoonose sistêmica grave, causada pelo protozoário intracelular obrigatório Leishmania infantum. Os cães são considerados os principais reservatórios da doença e podem desenvolver desde a infecção assintomática, a uma variedade de sinais clínicos, tais como linfadenopatia periférica, perda de peso, letargia, lesões ulcerativas de pele e febre. Alterações oculares, ortopédicas e neurológicas também podem ser observadas associadas à LVC, tais como ceratoconjuntivite, uveíte, convulsões, alterações comportamentais, artralgia e claudicação. Tal fato inclui a doença no diagnóstico diferencial das afecções oftálmicas, osteoarticulares e neurológicas, principalmente em áreas consideradas endêmicas para a infecção. O objetivo deste trabalho foi realizar o diagnóstico da leishmaniose visceral canina utilizando amostras de conjuntiva, humor aquoso, líquido sinovial, membrana sinovial e líquido cefalorraquidiano (LCR). O teste ELISA foi aplicado em amostras de LCR, líquido sinovial e humor aquoso; e avaliações histológica e imunohistoquímica foram realizadas na conjuntiva e na membrana sinovial. As amostras foram coletadas de 20 cães sorologicamente positivos para leishmaniose visceral. Através do Ensaio Imunoenzimático foi possível detectar anticorpos IgG anti-Leishmania em uma das amostras de LCR e de humor aquoso, e em seis amostras de líquido sinovial. A imunohistoquímica apresentou marcação positiva de formas amastigotas de L. infantum em cinco membranas sinoviais e cinco conjuntivas estudadas. Diante dos resultados, conclui-se que formas amastigotas de Leishmania sp. são encontradas parasitando a membrana sinovial e a conjuntiva de cães sorologicamente positivos para a doença, bem como anticorpos IgG específicos no humor aquoso, no LCR e no líquido sinovial desses animais.Submitted by (edna.saturno@ufrpe.br) on 2016-10-18T15:00:33Z No. of bitstreams: 1 Maira Santos Severo.pdf: 1331338 bytes, checksum: 2812cbd8bcd64b6d50c6a5db7306b5d2 (MD5)Made available in DSpace on 2016-10-18T15:00:33Z (GMT). No. of bitstreams: 1 Maira Santos Severo.pdf: 1331338 bytes, checksum: 2812cbd8bcd64b6d50c6a5db7306b5d2 (MD5) Previous issue date: 2012-02-13application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência VeterináriaUFRPEBrasilDepartamento de Medicina VeterináriaLeishmanioseCãoDiagnósticoCIENCIAS AGRARIAS::MEDICINA VETERINARIADiagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidianoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-3061482854177903105600600600-3020210563763616780453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5747/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALMaira Santos Severo.pdfMaira Santos Severo.pdfapplication/pdf1331338http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5747/2/Maira+Santos+Severo.pdf2812cbd8bcd64b6d50c6a5db7306b5d2MD52tede2/57472018-06-14 11:05:24.577oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:33:28.792857Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
title |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
spellingShingle |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano SEVERO, Maíra Santos Leishmaniose Cão Diagnóstico CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
title_full |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
title_fullStr |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
title_full_unstemmed |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
title_sort |
Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano |
author |
SEVERO, Maíra Santos |
author_facet |
SEVERO, Maíra Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
FAUSTINO, Maria Aparecida da Gloria |
dc.contributor.advisor-co1.fl_str_mv |
ALVES, Leucio Câmara |
dc.contributor.referee1.fl_str_mv |
SANTOS, Jamile Prado dos |
dc.contributor.referee2.fl_str_mv |
LIRA, Nair Silva Cavalcanti de |
dc.contributor.referee3.fl_str_mv |
RAMOS, Carlos Alberto do Nascimento |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5360635816108486 |
dc.contributor.author.fl_str_mv |
SEVERO, Maíra Santos |
contributor_str_mv |
FAUSTINO, Maria Aparecida da Gloria ALVES, Leucio Câmara SANTOS, Jamile Prado dos LIRA, Nair Silva Cavalcanti de RAMOS, Carlos Alberto do Nascimento |
dc.subject.por.fl_str_mv |
Leishmaniose Cão Diagnóstico |
topic |
Leishmaniose Cão Diagnóstico CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Canine Visceral Leishmaniasis (CVL) is a severe systemic zoonosos caused by the obligate intracellular protozoan Leishmania infantum. The dogs are the major reservoir of the disease and may develop since asymptomatic infection to a variety of clinical signs, such as peripheral lymphadenopathy, weight loss, lethargy, ulcerative lesions of the skin and fever. Ocular, orthopedic and neurological disorders can also be seen associated with CVL, such as keratitis, seizures, behavioral changes, joint pain and lameness. This fact includes the disease in the differential diagnosis of ophthalmic disorders, osteoarticular and nervous system, especially in endemic areas to infection. The aim of this study was the diagnosis of canine visceral leishmaniasis using samples of conjunctiva, aqueous humor, synovial fluid, synovial membrane and cerebrospinal fluid (CSF). The ELISA test was applied to samples of CSF, synovial fluid and aqueous humor, and histological and immunohistochemical evaluations were performed in the conjunctiva and in the synovial membrane of these animals. The samples were collected from 20 dogs serologically positive for visceral leishmaniasis. Through the immunoenzymatic assay could detect anti-Leishmania IgG antibodies in a sample of CSF and aqueous humor, and six samples of synovial fluid. Immunohistochemistry showed positive staining of amastigotes of L. infantum five synovial membranes and connective five studied. From the results, we conclude that amastigotes of Leishmania sp. are found parasitizing the synovial membrane and the conjunctiva of dogs serologically positive for the disease, as well as specific IgG antibodies in aqueous humor, CSF and synovial fluid of these animals. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-13 |
dc.date.accessioned.fl_str_mv |
2016-10-18T15:00:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SEVERO, Maíra Santos. Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano. 2012. 80 f.Tese ( Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5747 |
identifier_str_mv |
SEVERO, Maíra Santos. Diagnóstico da leishmaniose visceral canina por meio de amostras de conjuntiva ocular, humor aquoso, membrana sinovial, líquido sinovial e líquido cefalorraquidiano. 2012. 80 f.Tese ( Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5747 |
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por |
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por |
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600 600 600 |
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Universidade Federal Rural de Pernambuco |
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UFRPE |
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Universidade Federal Rural de Pernambuco |
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