Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909

Detalhes bibliográficos
Autor(a) principal: OLIVEIRA, Fabiana Felix de
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4519
Resumo: The present study it aimed at to describe the ocular development, with emphasis in the retina of the Betta splendens fish, an ornamental fish that presents an extra-embryonic development, favoring research that involves one better agreement of its embryology and studies on degenerations and regenerations, ontogeny and differentiation occured in the eye, this research was carried through of the embryonic period up to ten days post-eclosão. The complete development of the eye when it has the eclosion of the animal. For the study of the stages of development the animals had been anesthetized in water with Eugenol 50ppm, were fixed in a solution of 4% paraformaldehyde in phosphate buffer, dehydrated and infiltrated with Paraplast Plus. After that, confectioned sections of 1-5μm of thickness and stained with HE, PAS and staining TUNEL. As result was possible to observe that the eyes had appeared of evaginações of diencéfalo, forming the optic cup. Neuroectoderm, the mesoderm and the ectoderm of the surface are involved in the formation of the eye. The staining TUNEL identified to process of apoptotic cells in the retina and lens, proving that it has a reduction of cells during the development of the animal. In the adult fish, through the chemistry it was possible to observe the morphology and the cellular arrangement of the photoreceptors.
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spelling SÁ, Fabrício Bezerra deEVÊNCIO NETO, JoaquimMENDONÇA, Fábio de SouzaMEDEIROS, Juliana Pinto dehttp://lattes.cnpq.br/0237081682404241OLIVEIRA, Fabiana Felix de2016-05-25T12:57:30Z2012-05-25OLIVEIRA, Fabiana Felix de. Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909. 2012. 12 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4519The present study it aimed at to describe the ocular development, with emphasis in the retina of the Betta splendens fish, an ornamental fish that presents an extra-embryonic development, favoring research that involves one better agreement of its embryology and studies on degenerations and regenerations, ontogeny and differentiation occured in the eye, this research was carried through of the embryonic period up to ten days post-eclosão. The complete development of the eye when it has the eclosion of the animal. For the study of the stages of development the animals had been anesthetized in water with Eugenol 50ppm, were fixed in a solution of 4% paraformaldehyde in phosphate buffer, dehydrated and infiltrated with Paraplast Plus. After that, confectioned sections of 1-5μm of thickness and stained with HE, PAS and staining TUNEL. As result was possible to observe that the eyes had appeared of evaginações of diencéfalo, forming the optic cup. Neuroectoderm, the mesoderm and the ectoderm of the surface are involved in the formation of the eye. The staining TUNEL identified to process of apoptotic cells in the retina and lens, proving that it has a reduction of cells during the development of the animal. In the adult fish, through the chemistry it was possible to observe the morphology and the cellular arrangement of the photoreceptors.O presente estudo visou descrever o desenvolvimento ocular, com ênfase na retina do Betta splendens, um peixe ornamental que apresenta um desenvolvimento extra-embrionário, o que favorece pesquisas que envolvam um melhor entendimento da sua embriologia e estudos relacionados ao conhecimento de degenerações e regenerações ocorridas no olho, à pesquisa foi realizada do período embrionário até dez dias pós eclosão. O desenvolvimento do olho já esta completo quando o animal eclode por ser um órgão de extrema importância para locomoção, reprodução, alimentação e defesa deste em relação ao predador. Para o estudo das etapas de desenvolvimento os animais foram anestesiados com Eugenol, sendo utilizado 50ppm em água, perfundidos com Paraformaldeido a 4% em Tampão Fosfato, desidratados e embebidos em Paraplast Plus. Foram confeccionados cortes histológicos com 5 e 1μm de espessura e corados com HE, PAS e submetidos ao método TUNEL. Como resultado foi possível observar que os olhos surgiram de evaginações do diencéfalo, formando o cálice óptico, o neuroectoderma, o mesoderma e o ectoderma da superfície estão envolvidos na formação do olho. O método TUNEL identificou processo de apoptose na retina e cristalino, comprovando que há uma redução de células durante o desenvolvimento do animal. No peixe adulto, através da histoquímica foi possível observar a morfologia e o arranjo celular dos fotorreceptores.Submitted by (lucia.rodrigues@ufrpe.br) on 2016-05-25T12:57:30Z No. of bitstreams: 1 Fabiana Felix de Oliveira.pdf: 1456917 bytes, checksum: 0e4831795041d99c98c8196d60199a9b (MD5)Made available in DSpace on 2016-05-25T12:57:30Z (GMT). No. of bitstreams: 1 Fabiana Felix de Oliveira.pdf: 1456917 bytes, checksum: 0e4831795041d99c98c8196d60199a9b (MD5) Previous issue date: 2012-05-25Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalDesenvolvimento ocularRetinaAlevinoPeixeBetta splendensOcular developmentFishBetta splendensCIENCIAS AGRARIAS::MEDICINA VETERINARIADesenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600600-89223641879873962044536702642350173193590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4519/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51ORIGINALFabiana Felix de Oliveira.pdfFabiana Felix de Oliveira.pdfapplication/pdf1456917http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4519/2/Fabiana+Felix+de+Oliveira.pdf0e4831795041d99c98c8196d60199a9bMD52tede2/45192018-08-30 12:48:40.964oai:tede2:tede2/4519Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSP1BSSUEgTElDRU4/QQpFc3RhIGxpY2VuP2EgZGUgZXhlbXBsbyA/IGZvcm5lY2lkYSBhcGVuYXMgcGFyYSBmaW5zIGluZm9ybWF0aXZvcy4KCkxJQ0VOP0EgREUgRElTVFJJQlVJPz9PIE4/Ty1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YT8/byBkZXN0YSBsaWNlbj9hLCB2b2M/IChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSA/IFVuaXZlcnNpZGFkZSAKWFhYIChTaWdsYSBkYSBVbml2ZXJzaWRhZGUpIG8gZGlyZWl0byBuP28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsICB0cmFkdXppciAoY29uZm9ybWUgZGVmaW5pZG8gYWJhaXhvKSwgZS9vdSAKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhPz9vIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyP25pY28gZSAKZW0gcXVhbHF1ZXIgbWVpbywgaW5jbHVpbmRvIG9zIGZvcm1hdG9zID91ZGlvIG91IHY/ZGVvLgoKVm9jPyBjb25jb3JkYSBxdWUgYSBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZT9kbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhPz9vLgoKVm9jPyB0YW1iP20gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGM/cGlhIGEgc3VhIHRlc2Ugb3UgCmRpc3NlcnRhPz9vIHBhcmEgZmlucyBkZSBzZWd1cmFuP2EsIGJhY2stdXAgZSBwcmVzZXJ2YT8/by4KClZvYz8gZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byA/IG9yaWdpbmFsIGUgcXVlIHZvYz8gdGVtIG8gcG9kZXIgZGUgY29uY2VkZXIgb3MgZGlyZWl0b3MgY29udGlkb3MgCm5lc3RhIGxpY2VuP2EuIFZvYz8gdGFtYj9tIGRlY2xhcmEgcXVlIG8gZGVwP3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/P28gbj9vLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3U/bS4KCkNhc28gYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jPyBuP28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jPyAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzcz9vIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgPyBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgCm9zIGRpcmVpdG9zIGFwcmVzZW50YWRvcyBuZXN0YSBsaWNlbj9hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Q/IGNsYXJhbWVudGUgCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlP2RvIGRhIHRlc2Ugb3UgZGlzc2VydGE/P28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQT8/TyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0M/TklPIE9VIApBUE9JTyBERSBVTUEgQUc/TkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTj9PIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0M/IERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJUz9PIENPTU8gClRBTUI/TSBBUyBERU1BSVMgT0JSSUdBPz9FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgc2UgY29tcHJvbWV0ZSBhIGlkZW50aWZpY2FyIGNsYXJhbWVudGUgbyBzZXUgbm9tZSAocykgb3UgbyhzKSBub21lKHMpIGRvKHMpIApkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGE/P28sIGUgbj9vIGZhcj8gcXVhbHF1ZXIgYWx0ZXJhPz9vLCBhbD9tIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2VuP2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:31:52.660996Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
title Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
spellingShingle Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
OLIVEIRA, Fabiana Felix de
Desenvolvimento ocular
Retina
Alevino
Peixe
Betta splendens
Ocular development
Fish
Betta splendens
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
title_full Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
title_fullStr Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
title_full_unstemmed Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
title_sort Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909
author OLIVEIRA, Fabiana Felix de
author_facet OLIVEIRA, Fabiana Felix de
author_role author
dc.contributor.advisor1.fl_str_mv SÁ, Fabrício Bezerra de
dc.contributor.referee1.fl_str_mv EVÊNCIO NETO, Joaquim
dc.contributor.referee2.fl_str_mv MENDONÇA, Fábio de Souza
dc.contributor.referee3.fl_str_mv MEDEIROS, Juliana Pinto de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0237081682404241
dc.contributor.author.fl_str_mv OLIVEIRA, Fabiana Felix de
contributor_str_mv SÁ, Fabrício Bezerra de
EVÊNCIO NETO, Joaquim
MENDONÇA, Fábio de Souza
MEDEIROS, Juliana Pinto de
dc.subject.por.fl_str_mv Desenvolvimento ocular
Retina
Alevino
Peixe
topic Desenvolvimento ocular
Retina
Alevino
Peixe
Betta splendens
Ocular development
Fish
Betta splendens
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv Betta splendens
Ocular development
Fish
Betta splendens
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description The present study it aimed at to describe the ocular development, with emphasis in the retina of the Betta splendens fish, an ornamental fish that presents an extra-embryonic development, favoring research that involves one better agreement of its embryology and studies on degenerations and regenerations, ontogeny and differentiation occured in the eye, this research was carried through of the embryonic period up to ten days post-eclosão. The complete development of the eye when it has the eclosion of the animal. For the study of the stages of development the animals had been anesthetized in water with Eugenol 50ppm, were fixed in a solution of 4% paraformaldehyde in phosphate buffer, dehydrated and infiltrated with Paraplast Plus. After that, confectioned sections of 1-5μm of thickness and stained with HE, PAS and staining TUNEL. As result was possible to observe that the eyes had appeared of evaginações of diencéfalo, forming the optic cup. Neuroectoderm, the mesoderm and the ectoderm of the surface are involved in the formation of the eye. The staining TUNEL identified to process of apoptotic cells in the retina and lens, proving that it has a reduction of cells during the development of the animal. In the adult fish, through the chemistry it was possible to observe the morphology and the cellular arrangement of the photoreceptors.
publishDate 2012
dc.date.issued.fl_str_mv 2012-05-25
dc.date.accessioned.fl_str_mv 2016-05-25T12:57:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv OLIVEIRA, Fabiana Felix de. Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909. 2012. 12 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4519
identifier_str_mv OLIVEIRA, Fabiana Felix de. Desenvolvimento do olho e diferenciação da retina em peixes Betta splendens Regan, 1909. 2012. 12 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4519
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dc.publisher.department.fl_str_mv Departamento de Morfologia e Fisiologia Animal
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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