Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5606 |
Resumo: | The objective of this work to study the occurrence of anti-Toxoplasma gondii (T. gondii) and Leptospira spp. in pig killed in the Micro Valley Ipojuca Wasteland of Pernambuco We collected blood samples from 305 animals from 11 municipalities. Sera were submitted to the Immunofluorescence Assay (IFA) for detection of anti-T. gondii, by adopting a cutoff of 1:64. In animals positive for IFA was used the PCR technique to detect the DNA of T. gondii in fragments of heart. To search for anti Leptospira spp. used the microscopic agglutination test (MAT). Among all samples, we observed that thirty-eight 38/305 (12.46%) were positive for anti-T. gondii and 267/305 (87.54%) were negative, distributed in 11 cities. Of the animals positive serology, 21/38 (55.26%) were also positive in PCR. Results related to Leptospira spp. showed that 78/305 (25.57%) positive samples and 227/305 (74.42%) negative, distributed in 11 municipalities. The most frequent serovars were Icterohaemorrhagiae, Copenhageni, Djasiman and with frequencies of 55.12%, 17.94% and 6.41% respectively. It was concluded that pigs were beaten in the region exposed to infection by these agents and raises a discussion about the health risks of food handlers and consumers of carcasses and the need to minimize them through the implementation of surveillance programs and corrective actions in region studied. |
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MOTA, Rinaldo AparecidoBIOVETECH, Érica Paes Barreto Xavier de MoraesVALENÇA, Rômulo Menna BarretoPINHEIRO JÚNIOR, José Wiltonhttp://lattes.cnpq.br/5256493441853885CAVALCANTI, Erika Fernanda Torres Samico Fernandes2016-10-06T13:45:04Z2011-02-25CAVALCANTI, Erika Fernanda Torres Samico Fernandes. Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco. 2011. 84 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5606The objective of this work to study the occurrence of anti-Toxoplasma gondii (T. gondii) and Leptospira spp. in pig killed in the Micro Valley Ipojuca Wasteland of Pernambuco We collected blood samples from 305 animals from 11 municipalities. Sera were submitted to the Immunofluorescence Assay (IFA) for detection of anti-T. gondii, by adopting a cutoff of 1:64. In animals positive for IFA was used the PCR technique to detect the DNA of T. gondii in fragments of heart. To search for anti Leptospira spp. used the microscopic agglutination test (MAT). Among all samples, we observed that thirty-eight 38/305 (12.46%) were positive for anti-T. gondii and 267/305 (87.54%) were negative, distributed in 11 cities. Of the animals positive serology, 21/38 (55.26%) were also positive in PCR. Results related to Leptospira spp. showed that 78/305 (25.57%) positive samples and 227/305 (74.42%) negative, distributed in 11 municipalities. The most frequent serovars were Icterohaemorrhagiae, Copenhageni, Djasiman and with frequencies of 55.12%, 17.94% and 6.41% respectively. It was concluded that pigs were beaten in the region exposed to infection by these agents and raises a discussion about the health risks of food handlers and consumers of carcasses and the need to minimize them through the implementation of surveillance programs and corrective actions in region studied.Objetivou-se com este trabalho estudar a ocorrência de anticorpos anti-Toxoplasma gondii (T. gondii) e Leptospira spp. em suínos abatidos na Microrregião do Vale do Ipojuca, Agreste do Estado de Pernambuco. Foram coletadas 305 amostras de sangue de animais provenientes de 11 municípios. Os soros foram submetidos à Reação de Imunofluorescência Indireta (RIFI) para pesquisa de anticorpos anti-T. gondii, adotando-se ponto de corte de 1:64. Nos animais positivos para RIFI foi utilizada a técnica da PCR para detectar o DNA do T. gondii em fragmentos de coração. Para pesquisa de anticorpos anti-Leptospira spp. utilizou-se a técnica de Soroaglutinação Microscópica (SAM). Das amostras analisadas, observou-se que 38/305 (12,46%) foram positivas para anticorpos anti-T. gondii e 267/305 (87,54%) foram negativas, distribuídas em 11 cidades. Dos animais positivos na sorologia, 21/38 (55,26%) também foram positivos na PCR. Os resultados referentes à infecção por Leptospira spp. demonstraram que 78/305 (25,57%) amostras foram positivas e 227/305 (74,42%) negativas, distribuídas nos 11 municípios estudados. Os sorovares mais frequentes foram Icterohaemorrhagiae, Copenhageni e Djasiman com frequências de 55,12%, 17,94% e 6,41%, respectivamente. Conclui-se que os suínos abatidos nessa região foram expostos à infecção por esses agentes e levanta uma discussão sobre os riscos à saúde dos manipuladores das carcaças e dos consumidores e a necessidade de minimizá-los por meio da implantação de programas de vigilância e ações corretivas na região estudada.Submitted by (edna.saturno@ufrpe.br) on 2016-10-06T13:45:04Z No. of bitstreams: 1 Erika Fernanda Torres Samico Fernandes Cavalcanti.pdf: 636532 bytes, checksum: 2f815e6a83dda45939bd553b7269f4f7 (MD5)Made available in DSpace on 2016-10-06T13:45:04Z (GMT). No. of bitstreams: 1 Erika Fernanda Torres Samico Fernandes Cavalcanti.pdf: 636532 bytes, checksum: 2f815e6a83dda45939bd553b7269f4f7 (MD5) Previous issue date: 2011-02-25Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência VeterináriaUFRPEBrasilDepartamento de Medicina VeterináriaSuínoAbatedouroLeptospira spp.Toxoplasma gondiiLeptospirosisPigSerologySlaughterhouseCIENCIAS AGRARIAS::MEDICINA VETERINARIAPesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambucoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3061482854177903105600600600600-30202105637636167804536702642350173192075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALErika Fernanda Torres Samico Fernandes Cavalcanti.pdfErika Fernanda Torres Samico Fernandes Cavalcanti.pdfapplication/pdf636532http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5606/2/Erika+Fernanda+Torres+Samico+Fernandes+Cavalcanti.pdf2f815e6a83dda45939bd553b7269f4f7MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5606/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/56062016-10-06 10:45:04.826oai:tede2:tede2/5606Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2016-10-06T13:45:04Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
title |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
spellingShingle |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco CAVALCANTI, Erika Fernanda Torres Samico Fernandes Suíno Abatedouro Leptospira spp. Toxoplasma gondii Leptospirosis Pig Serology Slaughterhouse CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
title_full |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
title_fullStr |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
title_full_unstemmed |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
title_sort |
Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco |
author |
CAVALCANTI, Erika Fernanda Torres Samico Fernandes |
author_facet |
CAVALCANTI, Erika Fernanda Torres Samico Fernandes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
MOTA, Rinaldo Aparecido |
dc.contributor.referee1.fl_str_mv |
BIOVETECH, Érica Paes Barreto Xavier de Moraes |
dc.contributor.referee2.fl_str_mv |
VALENÇA, Rômulo Menna Barreto |
dc.contributor.referee3.fl_str_mv |
PINHEIRO JÚNIOR, José Wilton |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5256493441853885 |
dc.contributor.author.fl_str_mv |
CAVALCANTI, Erika Fernanda Torres Samico Fernandes |
contributor_str_mv |
MOTA, Rinaldo Aparecido BIOVETECH, Érica Paes Barreto Xavier de Moraes VALENÇA, Rômulo Menna Barreto PINHEIRO JÚNIOR, José Wilton |
dc.subject.por.fl_str_mv |
Suíno Abatedouro Leptospira spp. Toxoplasma gondii Leptospirosis |
topic |
Suíno Abatedouro Leptospira spp. Toxoplasma gondii Leptospirosis Pig Serology Slaughterhouse CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Pig Serology Slaughterhouse |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The objective of this work to study the occurrence of anti-Toxoplasma gondii (T. gondii) and Leptospira spp. in pig killed in the Micro Valley Ipojuca Wasteland of Pernambuco We collected blood samples from 305 animals from 11 municipalities. Sera were submitted to the Immunofluorescence Assay (IFA) for detection of anti-T. gondii, by adopting a cutoff of 1:64. In animals positive for IFA was used the PCR technique to detect the DNA of T. gondii in fragments of heart. To search for anti Leptospira spp. used the microscopic agglutination test (MAT). Among all samples, we observed that thirty-eight 38/305 (12.46%) were positive for anti-T. gondii and 267/305 (87.54%) were negative, distributed in 11 cities. Of the animals positive serology, 21/38 (55.26%) were also positive in PCR. Results related to Leptospira spp. showed that 78/305 (25.57%) positive samples and 227/305 (74.42%) negative, distributed in 11 municipalities. The most frequent serovars were Icterohaemorrhagiae, Copenhageni, Djasiman and with frequencies of 55.12%, 17.94% and 6.41% respectively. It was concluded that pigs were beaten in the region exposed to infection by these agents and raises a discussion about the health risks of food handlers and consumers of carcasses and the need to minimize them through the implementation of surveillance programs and corrective actions in region studied. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-25 |
dc.date.accessioned.fl_str_mv |
2016-10-06T13:45:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CAVALCANTI, Erika Fernanda Torres Samico Fernandes. Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco. 2011. 84 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5606 |
identifier_str_mv |
CAVALCANTI, Erika Fernanda Torres Samico Fernandes. Pesquisa de Toxoplasma gondii e anticorpos anti–Leptospira spp. em suínos abatidos no agreste do Estado de Pernambuco. 2011. 84 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5606 |
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por |
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por |
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openAccess |
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Universidade Federal Rural de Pernambuco |
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Programa de Pós-Graduação em Ciência Veterinária |
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UFRPE |
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Departamento de Medicina Veterinária |
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Universidade Federal Rural de Pernambuco |
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