Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4657 |
Resumo: | Glucocorticoids are used in prenatal treatments to speed up the pulmonary development in cases of prematurity risk. The administration of glucocorticoids is commonly associated with change in the production and distribution of blood cells, hyperglycemia and change in carbohydrates metabolization in target-tissue, as liver. The present research has subjected to evaluate the effect of dexamethasone application in early and mid- pregnancy of rats over hematologic, biochemist and histochemistry parameters of the offspring. A Number of 12 albinic rats was divided in: group I rats treated with dexametasona 1 to 7 pregnancy day and with placebo 8 to 14 pregnancy day; group II rats treated with placebo 1 to 7 pregnancy day and with dexametasona 8 to 14 pregnancy day; group III rats treated with dexametasona to 1 to 14 pregnancy day with dexamethasone concentration reduction of 15% to each 2 days and group IV rats treated with placebo 1 to 14 pregnancy day. Decadron® commercial formularization was managed for intraperitoneal injection daily in the dose of 0.8mg/kg/animal. For analysis of the hematologic parameters and level of full blood carbohydrates, blood samples was taken for rats in 7, 14 and 21 pregnancy day and for the offspring in 5, 10 and 15 post-birth day. For assessment of offspring glycogen liver storage, histological sections were stained by Schiff’s Periodic Acid. The results of the present research point in respect to a time-dependent effect of dexamethasone administration during the pregnancy, leading to a distinct secular alterations in the hematology as lymphopenia, eosinopenia and neutrophilia at the moments of the administration, being reversible until the end of the pregnancy when performed in early third of pregnancy and changes in plasma levels of full blood carbohydrates in the matrices and offspring are more evident in the longest treatment. |
id |
URPE_b9896121f3e3b6afce7831026908eeb2 |
---|---|
oai_identifier_str |
oai:tede2:tede2/4657 |
network_acronym_str |
URPE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
repository_id_str |
|
spelling |
TEIXEIRA, Álvaro Aguiar CoelhoSOARES, Anísio FranciscoTEIXEIRA, Valéria WanderleyCORREIA, Marcos Joséhttp://lattes.cnpq.br/8975529334104952VILAÇA JÚNIOR, Paulo Estevão Araújo2016-06-09T14:00:00Z2011-02-24VILAÇA JÚNIOR, Paulo Estevão Araújo. Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos. 2011. 68 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4657Glucocorticoids are used in prenatal treatments to speed up the pulmonary development in cases of prematurity risk. The administration of glucocorticoids is commonly associated with change in the production and distribution of blood cells, hyperglycemia and change in carbohydrates metabolization in target-tissue, as liver. The present research has subjected to evaluate the effect of dexamethasone application in early and mid- pregnancy of rats over hematologic, biochemist and histochemistry parameters of the offspring. A Number of 12 albinic rats was divided in: group I rats treated with dexametasona 1 to 7 pregnancy day and with placebo 8 to 14 pregnancy day; group II rats treated with placebo 1 to 7 pregnancy day and with dexametasona 8 to 14 pregnancy day; group III rats treated with dexametasona to 1 to 14 pregnancy day with dexamethasone concentration reduction of 15% to each 2 days and group IV rats treated with placebo 1 to 14 pregnancy day. Decadron® commercial formularization was managed for intraperitoneal injection daily in the dose of 0.8mg/kg/animal. For analysis of the hematologic parameters and level of full blood carbohydrates, blood samples was taken for rats in 7, 14 and 21 pregnancy day and for the offspring in 5, 10 and 15 post-birth day. For assessment of offspring glycogen liver storage, histological sections were stained by Schiff’s Periodic Acid. The results of the present research point in respect to a time-dependent effect of dexamethasone administration during the pregnancy, leading to a distinct secular alterations in the hematology as lymphopenia, eosinopenia and neutrophilia at the moments of the administration, being reversible until the end of the pregnancy when performed in early third of pregnancy and changes in plasma levels of full blood carbohydrates in the matrices and offspring are more evident in the longest treatment.Glicocorticóides são utilizados em tratamentos pré-natais para acelerar o desenvolvimento pulmonar em casos de risco de prematuridade. A administração de glicocorticóides é comumente associada com alterações na produção e distribuição de células sanguíneas, elevação na glicemia e alterações na metabolização de carboidratos em tecidos-alvo como o fígado. A presente pesquisa teve o objetivo de avaliar o efeito da aplicação de dexametasona no início da gestação de ratas, sobre parâmetros hematológicos, bioquímicos e histoquímicos da prole. Foram utilizadas 12 ratas albinas divididas em: grupo I com ratas tratadas com dexametasona do 1º ao 7º dia de gestação e com placebo a partir do 8º dia até o 14º dia de gestação; grupo II com ratas tratadas com placebo 1º ao 7º dia de gestação e com dexametasona do 8º ao 14º dia de gestação; grupo III com ratas tratadas com dexametasona do 1º ao 14º dia de gestação com progressiva redução de 15% da concentração de dexametasona a cada 2 dias e grupo IV com ratas tratadas com placebodo 1º até o 14º dia de gestação.Foi utilizada a formulação comercial Decadron® administrada por via intraperitoneal, em uma aplicação diária na dose de 0.8mg/kg/animal. Para a análise dos parâmetros hematológicos e níveis de carboidratos totais foram coletadas amostras de sangue nas ratas no 7o, 14o e 21o dia de gestação e na prole no 5o, 10 o e 15 o dia pós-natal. Para a verificação das reservas de glicogênio hepático da prole, cortes histológicos foram corados pelo Ácido Periódico de Schiff. Os resultados da presente pesquisa apontam para um efeito tempo-dependente da administração de dexametasona durante a prenhez, levando a alterações temporais distintas na hematologia como linfopenia, eosinopenia e neutrofilía nos momentos da administração, sendo reversíveis até o fim da gestação quando realizadas no terço inicial da gestação e alteração no nível plasmático de carboidratos totais nas matrizes e na prole mais evidente no tratamento mais duradouro.Submitted by (lucia.rodrigues@ufrpe.br) on 2016-06-09T14:00:00Z No. of bitstreams: 1 Paulo Estevao Araujo Vilaca Junior.pdf: 1625360 bytes, checksum: a42cfa474fa1dc77d887e1bea594bbdd (MD5)Made available in DSpace on 2016-06-09T14:00:00Z (GMT). No. of bitstreams: 1 Paulo Estevao Araujo Vilaca Junior.pdf: 1625360 bytes, checksum: a42cfa474fa1dc77d887e1bea594bbdd (MD5) Previous issue date: 2011-02-24application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalDexametasonaTratamento pré-natalCarboidratosRatoLiverDexamethasonePrenatal treatmentCarbohydratesRatsCIENCIAS AGRARIAS::MEDICINA VETERINARIAEfeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4657/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALPaulo Estevao Araujo Vilaca Junior.pdfPaulo Estevao Araujo Vilaca Junior.pdfapplication/pdf1625360http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4657/2/Paulo+Estevao+Araujo+Vilaca+Junior.pdfa42cfa474fa1dc77d887e1bea594bbddMD52tede2/46572024-07-05 15:02:07.703oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-07-05T18:02:07Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
title |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
spellingShingle |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos VILAÇA JÚNIOR, Paulo Estevão Araújo Dexametasona Tratamento pré-natal Carboidratos Rato Liver Dexamethasone Prenatal treatment Carbohydrates Rats CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
title_full |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
title_fullStr |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
title_full_unstemmed |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
title_sort |
Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos |
author |
VILAÇA JÚNIOR, Paulo Estevão Araújo |
author_facet |
VILAÇA JÚNIOR, Paulo Estevão Araújo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
TEIXEIRA, Álvaro Aguiar Coelho |
dc.contributor.advisor-co1.fl_str_mv |
SOARES, Anísio Francisco |
dc.contributor.advisor-co2.fl_str_mv |
TEIXEIRA, Valéria Wanderley |
dc.contributor.referee1.fl_str_mv |
CORREIA, Marcos José |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8975529334104952 |
dc.contributor.author.fl_str_mv |
VILAÇA JÚNIOR, Paulo Estevão Araújo |
contributor_str_mv |
TEIXEIRA, Álvaro Aguiar Coelho SOARES, Anísio Francisco TEIXEIRA, Valéria Wanderley CORREIA, Marcos José |
dc.subject.por.fl_str_mv |
Dexametasona Tratamento pré-natal Carboidratos Rato |
topic |
Dexametasona Tratamento pré-natal Carboidratos Rato Liver Dexamethasone Prenatal treatment Carbohydrates Rats CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Liver Dexamethasone Prenatal treatment Carbohydrates Rats |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Glucocorticoids are used in prenatal treatments to speed up the pulmonary development in cases of prematurity risk. The administration of glucocorticoids is commonly associated with change in the production and distribution of blood cells, hyperglycemia and change in carbohydrates metabolization in target-tissue, as liver. The present research has subjected to evaluate the effect of dexamethasone application in early and mid- pregnancy of rats over hematologic, biochemist and histochemistry parameters of the offspring. A Number of 12 albinic rats was divided in: group I rats treated with dexametasona 1 to 7 pregnancy day and with placebo 8 to 14 pregnancy day; group II rats treated with placebo 1 to 7 pregnancy day and with dexametasona 8 to 14 pregnancy day; group III rats treated with dexametasona to 1 to 14 pregnancy day with dexamethasone concentration reduction of 15% to each 2 days and group IV rats treated with placebo 1 to 14 pregnancy day. Decadron® commercial formularization was managed for intraperitoneal injection daily in the dose of 0.8mg/kg/animal. For analysis of the hematologic parameters and level of full blood carbohydrates, blood samples was taken for rats in 7, 14 and 21 pregnancy day and for the offspring in 5, 10 and 15 post-birth day. For assessment of offspring glycogen liver storage, histological sections were stained by Schiff’s Periodic Acid. The results of the present research point in respect to a time-dependent effect of dexamethasone administration during the pregnancy, leading to a distinct secular alterations in the hematology as lymphopenia, eosinopenia and neutrophilia at the moments of the administration, being reversible until the end of the pregnancy when performed in early third of pregnancy and changes in plasma levels of full blood carbohydrates in the matrices and offspring are more evident in the longest treatment. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-24 |
dc.date.accessioned.fl_str_mv |
2016-06-09T14:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VILAÇA JÚNIOR, Paulo Estevão Araújo. Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos. 2011. 68 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4657 |
identifier_str_mv |
VILAÇA JÚNIOR, Paulo Estevão Araújo. Efeito da administração pré-natal de dexametasona sobre parâmetros sanguíneos e desenvolvimento hepático na prole de ratos. 2011. 68 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4657 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-1510757014399315592 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-8922364187987396204 |
dc.relation.cnpq.fl_str_mv |
453670264235017319 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biociência Animal |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Morfologia e Fisiologia Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRPE instname:Universidade Federal Rural de Pernambuco (UFRPE) instacron:UFRPE |
instname_str |
Universidade Federal Rural de Pernambuco (UFRPE) |
instacron_str |
UFRPE |
institution |
UFRPE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
collection |
Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4657/1/license.txt http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4657/2/Paulo+Estevao+Araujo+Vilaca+Junior.pdf |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 a42cfa474fa1dc77d887e1bea594bbdd |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
_version_ |
1810102212355948544 |