Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7207 |
Resumo: | Calotropis procera, is a medicinal plant known in Pernambuco as “silk-cotton”. Is laticífer plant belonging to the family Apocynaceae broadly found in the Brazilian Northeast. Current literature data show that proteins obtained from its latex harbour anti-inflammatory action. In the present study, a protein fraction obtained by ion-exchange chromatography named LPPII, which is rich in cysteine proteases, had its immunmodulatory activity evaluated in cultures of peritoneal macrophages infected by Salmonella enterica Sor. Typhimurium. Macrophages were obtained from the peritoneal cavity of Swiss mice using RPMI culture medium containing antibiotics. The cells obtained were adjusted to 1 x 106 cell/mL and incubated at 37° C and 5% CO2, in cell culture plates, and the adherent macrophages used in the assays. The bacterial quantification assays were performed in a preventive manner, where the macrophages were treated with LPPII (1 μg/ mL or 10 μg/ mL) or LPPII+IAA (10 μg / mL) (inactivated with iodoacetamide) followed by infection with S. Typhimurium (1 x 108 CFU / mL), and in a curative manner, where macrophages were first infected with S. typhimurium (1 x 108 CFU / mL) followed by treatment with LPPII (1 μg/ mL or 10 μg/ mL) or LPPII+IAA (10 μg/ ml). The cell viability assay was performed curatively with macrophages infected with S. Typhimurium (1 x 108 CFU / ml). For IL1β, TNF, IL-6, iNOS and TLR-4 cytokine gene expression assays, macrophages were only treated with LPPII (1 μg/ mL or 10 μg/ mL) or LP+IAA (10 μg / mL), or bacterial LPS stimulation, followed by curative or preventative treatments with LPPII (1 μg/ mL or 10 μg/ mL) or LP+IAA (10 μg/ mL). The results show that macrophages infected with a S. Typhimurium C5 and treated with LPPII in a preventative or curative manner, reduced the number of viable bacteria in the intracellular environment. In this case, macrophages treated preventively with LPPII 10 μg/ mL, showed a significant decrease (p <0.05) in the amount of intracellular bacteria when compared to the control, macrophages without LPPII treatment. In the curative form, significant decrease of intracellular S. Typhimurium was observed in LPPII1μg/ mL-treated macrophages compared to control cells, without LPPII treatment. On the other hand, the groups treated with LPPII+IAA had a greater number of intracellular bacteria, suggesting the action of cysteine proteases on the observed antimicrobial effect. Curative treatment with LPPII also increased the viability of infected macrophages relative to the untreated control groups. Thus, groups treated with LPPII1μg/ mL obtained viability 14.74% greater than the control group without treatment, whereas macrophages treated with LPPII10μg/ mL obtained 24.11%. Groups of macrophages stimulated with LPS and then treated with LPPII had a reduction in the gene expression of the inflammatory cytokines TNF, IL1-β and IL-6, in addition to Toll-like receptor 4. Taken together, we found that LPPII obtained from latex of C. procera presents biomolecules with immunomodulatory activity beneficial to the control of S. Typhimurium infections. |
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LIMA FILHO, José Vitor MoreiraPORTO, Tatiana SouzaOLIVEIRA, Jaqueline Bianque deSILVA, Rafael de Freitas ehttp://lattes.cnpq.br/4128808335995892TAVARES, Lethicia Souza2018-04-20T15:09:26Z2017-02-24TAVARES, Lethicia Souza. Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos. 2017. 64 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7207Calotropis procera, is a medicinal plant known in Pernambuco as “silk-cotton”. Is laticífer plant belonging to the family Apocynaceae broadly found in the Brazilian Northeast. Current literature data show that proteins obtained from its latex harbour anti-inflammatory action. In the present study, a protein fraction obtained by ion-exchange chromatography named LPPII, which is rich in cysteine proteases, had its immunmodulatory activity evaluated in cultures of peritoneal macrophages infected by Salmonella enterica Sor. Typhimurium. Macrophages were obtained from the peritoneal cavity of Swiss mice using RPMI culture medium containing antibiotics. The cells obtained were adjusted to 1 x 106 cell/mL and incubated at 37° C and 5% CO2, in cell culture plates, and the adherent macrophages used in the assays. The bacterial quantification assays were performed in a preventive manner, where the macrophages were treated with LPPII (1 μg/ mL or 10 μg/ mL) or LPPII+IAA (10 μg / mL) (inactivated with iodoacetamide) followed by infection with S. Typhimurium (1 x 108 CFU / mL), and in a curative manner, where macrophages were first infected with S. typhimurium (1 x 108 CFU / mL) followed by treatment with LPPII (1 μg/ mL or 10 μg/ mL) or LPPII+IAA (10 μg/ ml). The cell viability assay was performed curatively with macrophages infected with S. Typhimurium (1 x 108 CFU / ml). For IL1β, TNF, IL-6, iNOS and TLR-4 cytokine gene expression assays, macrophages were only treated with LPPII (1 μg/ mL or 10 μg/ mL) or LP+IAA (10 μg / mL), or bacterial LPS stimulation, followed by curative or preventative treatments with LPPII (1 μg/ mL or 10 μg/ mL) or LP+IAA (10 μg/ mL). The results show that macrophages infected with a S. Typhimurium C5 and treated with LPPII in a preventative or curative manner, reduced the number of viable bacteria in the intracellular environment. In this case, macrophages treated preventively with LPPII 10 μg/ mL, showed a significant decrease (p <0.05) in the amount of intracellular bacteria when compared to the control, macrophages without LPPII treatment. In the curative form, significant decrease of intracellular S. Typhimurium was observed in LPPII1μg/ mL-treated macrophages compared to control cells, without LPPII treatment. On the other hand, the groups treated with LPPII+IAA had a greater number of intracellular bacteria, suggesting the action of cysteine proteases on the observed antimicrobial effect. Curative treatment with LPPII also increased the viability of infected macrophages relative to the untreated control groups. Thus, groups treated with LPPII1μg/ mL obtained viability 14.74% greater than the control group without treatment, whereas macrophages treated with LPPII10μg/ mL obtained 24.11%. Groups of macrophages stimulated with LPS and then treated with LPPII had a reduction in the gene expression of the inflammatory cytokines TNF, IL1-β and IL-6, in addition to Toll-like receptor 4. Taken together, we found that LPPII obtained from latex of C. procera presents biomolecules with immunomodulatory activity beneficial to the control of S. Typhimurium infections.Calotropis procera, planta medicinal conhecida popularmente em Pernambuco como algodão-de-seda. É uma planta laticífera que pertence à família Apocynaceae, sendo encontrada com facilidade no nordeste brasileiro. Dados da literatura corrente mostram que proteínas obtidas de seu látex possuem ação anti-inflamatória. Diante disso, neste trabalho, uma fração proteica obtida por cromatografia de troca iônica, chamada LPPII, rica em proteases cisteínicas, foi avaliada quanto à sua atividade imunomodulatória em culturas de macrófagos peritoneais infectadas por Salmonella enterica Sor. Typhimurium. Os macrófagos foram obtidos a partir da lavagem peritoneal de camundongos Swiss com meio de cultura RPMI contendo antibióticos penicilina e estreptomicina. As células do fluido obtido foram ajustadas a 1 x 106 cél/ mL e incubadas em estufa a 37ºC e CO2 5%, em placas de cultura de células, e os macrófagos aderentes utilizados nos ensaios. Os ensaios de quantificação bacteriana se deram de forma preventiva, onde os macrófagos foram tratados com LPPII (1 μg/mL ou 10 μg/mL) ou LPPII+IAA (10 μg/mL) (inativada com iodoacetamida) seguido de infecção com S. Typhimurium (1 x 108 UFC/mL), e de forma curativa, onde primeiro se deu infecção dos macrófagos por S. Typhimurium (1 x 108 UFC/mL) seguido de tratamento com LPPII (1 μg/mL ou 10 μg/mL) ou LPPII+IAA (10 μg/mL). O ensaio de viabilidade celular foi realizado de forma curativa com macrófagos infectados com S. Typhimurium (1 x 108 UFC/mL). Para ensaios de expressão gênica de citocinas IL1- β, TNF, IL-6, iNOS e TLR-4, os macrófagos receberam apenas tratamento com LPPII (1 μg/mL ou 10 μg/mL) ou LP+IAA (10 μg/mL), ou houve estímulo de LPS bacteriano, seguidos de tratamentos de forma curativa ou preventiva com LPPII (1 μg/mL ou 10 μg/mL) ou LP+IAA (10 μg/mL). Os resultados mostram que macrófagos infectados com uma cepa de S. Typhimurium C5 e tratados com LPPII de forma preventiva ou curativa, reduziram o número de bactérias viáveis no ambiente intracelular. Neste caso, macrófagos tratados de forma preventiva com LPPII 10 μg/mL, demonstraram diminuição significativa (p<0,05) na quantidade de bactérias intracelulares quando comparados ao controle, macrófagos sem tratamento com LPPII. Na forma curativa, observou-se diminuição significativa de S. Typhimurium intracelular em macrófagos tratados com LPPII1μg/mL, quando comparados ao controle, células sem tratamento com LPPII. Por outro lado, os grupos tratados com LPPII+IAA tiveram um maior número de bactérias intracelulares, sugerindo a ação das proteases cisteínicas no efeito antimicrobiano observado. O tratamento curativo com LPPII também aumentou a viabilidade dos macrófagos infectados em relação aos grupos controles sem tratamento. Deste modo, grupos tratados com LPPII1μg/mL obtiveram viabilidade 14,74% maior que o grupo controle sem tratamento, enquanto que macrófagos tratados com LPPII10μg/mL obtiveram 24,11%. Grupos de macrófagos estimulados com LPS e, a seguir, tratados com LPPII, tiveram redução na expressão gênica das citocinas inflamatórias TNF, IL1-β e IL-6, além de receptor do tipo Toll-4. Tomados esses resultados em conjunto, observamos que LPPII obtida do látex de C. procera apresenta biomoléculas com atividade imunomodulatória benéfica ao controle de infecções por S. Typhimurium.Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-04-20T15:09:26Z No. of bitstreams: 1 Lethicia Souza Tavares.pdf: 1585867 bytes, checksum: 5409eeb189a05f35fe911992f6371542 (MD5)Made available in DSpace on 2018-04-20T15:09:26Z (GMT). No. of bitstreams: 1 Lethicia Souza Tavares.pdf: 1585867 bytes, checksum: 5409eeb189a05f35fe911992f6371542 (MD5) Previous issue date: 2017-02-24Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalCalotropis proceraLátexProtease cisteínicaSalmonella entericaPlanta medicinalAtividade imunomodulatóriaCIENCIAS AGRARIAS::MEDICINA VETERINARIACaracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600600-8922364187987396204453670264235017319-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALLethicia Souza Tavares.pdfLethicia Souza Tavares.pdfapplication/pdf1585867http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7207/2/Lethicia+Souza+Tavares.pdf5409eeb189a05f35fe911992f6371542MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7207/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/72072018-04-20 12:09:26.306oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:35:20.913942Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
title |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
spellingShingle |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos TAVARES, Lethicia Souza Calotropis procera Látex Protease cisteínica Salmonella enterica Planta medicinal Atividade imunomodulatória CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
title_full |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
title_fullStr |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
title_full_unstemmed |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
title_sort |
Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos |
author |
TAVARES, Lethicia Souza |
author_facet |
TAVARES, Lethicia Souza |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
LIMA FILHO, José Vitor Moreira |
dc.contributor.referee1.fl_str_mv |
PORTO, Tatiana Souza |
dc.contributor.referee2.fl_str_mv |
OLIVEIRA, Jaqueline Bianque de |
dc.contributor.referee3.fl_str_mv |
SILVA, Rafael de Freitas e |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4128808335995892 |
dc.contributor.author.fl_str_mv |
TAVARES, Lethicia Souza |
contributor_str_mv |
LIMA FILHO, José Vitor Moreira PORTO, Tatiana Souza OLIVEIRA, Jaqueline Bianque de SILVA, Rafael de Freitas e |
dc.subject.por.fl_str_mv |
Calotropis procera Látex Protease cisteínica Salmonella enterica Planta medicinal Atividade imunomodulatória |
topic |
Calotropis procera Látex Protease cisteínica Salmonella enterica Planta medicinal Atividade imunomodulatória CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Calotropis procera, is a medicinal plant known in Pernambuco as “silk-cotton”. Is laticífer plant belonging to the family Apocynaceae broadly found in the Brazilian Northeast. Current literature data show that proteins obtained from its latex harbour anti-inflammatory action. In the present study, a protein fraction obtained by ion-exchange chromatography named LPPII, which is rich in cysteine proteases, had its immunmodulatory activity evaluated in cultures of peritoneal macrophages infected by Salmonella enterica Sor. Typhimurium. Macrophages were obtained from the peritoneal cavity of Swiss mice using RPMI culture medium containing antibiotics. The cells obtained were adjusted to 1 x 106 cell/mL and incubated at 37° C and 5% CO2, in cell culture plates, and the adherent macrophages used in the assays. The bacterial quantification assays were performed in a preventive manner, where the macrophages were treated with LPPII (1 μg/ mL or 10 μg/ mL) or LPPII+IAA (10 μg / mL) (inactivated with iodoacetamide) followed by infection with S. Typhimurium (1 x 108 CFU / mL), and in a curative manner, where macrophages were first infected with S. typhimurium (1 x 108 CFU / mL) followed by treatment with LPPII (1 μg/ mL or 10 μg/ mL) or LPPII+IAA (10 μg/ ml). The cell viability assay was performed curatively with macrophages infected with S. Typhimurium (1 x 108 CFU / ml). For IL1β, TNF, IL-6, iNOS and TLR-4 cytokine gene expression assays, macrophages were only treated with LPPII (1 μg/ mL or 10 μg/ mL) or LP+IAA (10 μg / mL), or bacterial LPS stimulation, followed by curative or preventative treatments with LPPII (1 μg/ mL or 10 μg/ mL) or LP+IAA (10 μg/ mL). The results show that macrophages infected with a S. Typhimurium C5 and treated with LPPII in a preventative or curative manner, reduced the number of viable bacteria in the intracellular environment. In this case, macrophages treated preventively with LPPII 10 μg/ mL, showed a significant decrease (p <0.05) in the amount of intracellular bacteria when compared to the control, macrophages without LPPII treatment. In the curative form, significant decrease of intracellular S. Typhimurium was observed in LPPII1μg/ mL-treated macrophages compared to control cells, without LPPII treatment. On the other hand, the groups treated with LPPII+IAA had a greater number of intracellular bacteria, suggesting the action of cysteine proteases on the observed antimicrobial effect. Curative treatment with LPPII also increased the viability of infected macrophages relative to the untreated control groups. Thus, groups treated with LPPII1μg/ mL obtained viability 14.74% greater than the control group without treatment, whereas macrophages treated with LPPII10μg/ mL obtained 24.11%. Groups of macrophages stimulated with LPS and then treated with LPPII had a reduction in the gene expression of the inflammatory cytokines TNF, IL1-β and IL-6, in addition to Toll-like receptor 4. Taken together, we found that LPPII obtained from latex of C. procera presents biomolecules with immunomodulatory activity beneficial to the control of S. Typhimurium infections. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-24 |
dc.date.accessioned.fl_str_mv |
2018-04-20T15:09:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TAVARES, Lethicia Souza. Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos. 2017. 64 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7207 |
identifier_str_mv |
TAVARES, Lethicia Souza. Caracterização imunomodulatória de proteases cisteínicas obtidas do látex de Calotropis procera em culturas de macrófagos. 2017. 64 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7207 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-1510757014399315592 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-8922364187987396204 |
dc.relation.cnpq.fl_str_mv |
453670264235017319 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biociência Animal |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Morfologia e Fisiologia Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRPE instname:Universidade Federal Rural de Pernambuco (UFRPE) instacron:UFRPE |
instname_str |
Universidade Federal Rural de Pernambuco (UFRPE) |
instacron_str |
UFRPE |
institution |
UFRPE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
collection |
Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7207/2/Lethicia+Souza+Tavares.pdf http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7207/1/license.txt |
bitstream.checksum.fl_str_mv |
5409eeb189a05f35fe911992f6371542 bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
_version_ |
1810102247461224448 |