Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7250 |
Resumo: | Finasteride and testosterone are hormones that have anti-androgenic action (inhibition of 5α-reductase) and androgen respectively, which can act as endocrine disruptors causing negative effects on aquatic animals and their offspring. Finasteride was initially developed to treat benign prostatic hyperplasia, however, was presented as a major discovery in the treatment of androgenetic alopecia (baldness) already the testosterone is the hormone responsible for secondary sexual characteristics and has the most active metabolite the dihydrotestosterone, main responsible for baldness. However, finasteride as well as testosterone are commonly used in various situations, both by men in the treatment against hair loss (finasteride) or sexual reversal of fish (testosterone), which increases the presence of these hormones in the aquatic environment. Once in the environment both finasteride and testosterone begin to have action of endocrine disruptors, being one of the most powerful aquatic pollutants. Because it has hydrophobic properties which reduces their bioavailability, such hormones can be complexed to cyclodextrins (CDs) to reduce this limitation, therefore its use in inclusion complexes would increase its solubility and absorption, and may change one or more hormonal pathways. In view of the foregoing, the present study aims to evaluate the toxic effects of the inclusion complex Finasteride β-cyclodextrin (β-CD) and Testosterone β-cyclodextrin (β-CD), through chronic exposure to sublethal concentrations under the embryos and adults of the zebrafish (Danio rerio). The work was aimed to evaluate the toxic effects of finasteride and testosterone free and complexed into cyclodextrin on the physiological and behavioral parameters of embryos and adults of the Danio rerio fish. The embryos were exposed for periods of 96 hours post-fertilization for the evaluation of mortality, teratogenic effects, and heart rate. The groups were divided into control, free finasteride (FIN) and testosterone (T) at the concentrations of 280, 560, 1120, 2240, 4480 ng/L, and finasteride (FIN-β-CD) and testosterone (T-β-CD) complexed into β-CD at concentrations of 280 and 4480 ng/L. Adults were exposed for a period of 60 days to evaluate behavioral changes and daily survival related to exposure to free finasteride (FIN) and testosterone (T) complexed into β-CD at concentrations 280 and 4480 ng/L. The embryos did not exhibit mortality at concentrations of 280 - 4480 ng/L in the all groups exposed. Similarly, it was not observed teratogenic effects in the groups exposed to different concentrations. In the heart frequencies of the embryos, significant variations were observed (p < 0 .05) when compared to the control group. Adults exposed to free andcomplexed finasteride and testosterone had significant alterations (p < 0.05) in the most active behaviors such as slow swim and fast swim, as well as in agonistic behaviors. It can be concluded that in tests with embryos, FIN and FIN-β-CD as well T and T- β-CD, may interfere in the heart rate and the exposure of adults to the same hormones promoted a reduction in their locomotor capacity and can increase in the aggressiveness of the animals. |
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CADENA, Pabyton GonçalvesCADENA, Marilia Ribeiro SalesAMORIM, Marleyne José Afonso Accioly LinsBEZERRA, Raquel PedrosaSOUZA, Paulo Eleutério dehttp://lattes.cnpq.br/7895981207021342COSTA, Sérgio Clementino da2018-05-16T13:17:57Z2018-02-28COSTA, Sérgio Clementino da. Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio. 2018. 54 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7250Finasteride and testosterone are hormones that have anti-androgenic action (inhibition of 5α-reductase) and androgen respectively, which can act as endocrine disruptors causing negative effects on aquatic animals and their offspring. Finasteride was initially developed to treat benign prostatic hyperplasia, however, was presented as a major discovery in the treatment of androgenetic alopecia (baldness) already the testosterone is the hormone responsible for secondary sexual characteristics and has the most active metabolite the dihydrotestosterone, main responsible for baldness. However, finasteride as well as testosterone are commonly used in various situations, both by men in the treatment against hair loss (finasteride) or sexual reversal of fish (testosterone), which increases the presence of these hormones in the aquatic environment. Once in the environment both finasteride and testosterone begin to have action of endocrine disruptors, being one of the most powerful aquatic pollutants. Because it has hydrophobic properties which reduces their bioavailability, such hormones can be complexed to cyclodextrins (CDs) to reduce this limitation, therefore its use in inclusion complexes would increase its solubility and absorption, and may change one or more hormonal pathways. In view of the foregoing, the present study aims to evaluate the toxic effects of the inclusion complex Finasteride β-cyclodextrin (β-CD) and Testosterone β-cyclodextrin (β-CD), through chronic exposure to sublethal concentrations under the embryos and adults of the zebrafish (Danio rerio). The work was aimed to evaluate the toxic effects of finasteride and testosterone free and complexed into cyclodextrin on the physiological and behavioral parameters of embryos and adults of the Danio rerio fish. The embryos were exposed for periods of 96 hours post-fertilization for the evaluation of mortality, teratogenic effects, and heart rate. The groups were divided into control, free finasteride (FIN) and testosterone (T) at the concentrations of 280, 560, 1120, 2240, 4480 ng/L, and finasteride (FIN-β-CD) and testosterone (T-β-CD) complexed into β-CD at concentrations of 280 and 4480 ng/L. Adults were exposed for a period of 60 days to evaluate behavioral changes and daily survival related to exposure to free finasteride (FIN) and testosterone (T) complexed into β-CD at concentrations 280 and 4480 ng/L. The embryos did not exhibit mortality at concentrations of 280 - 4480 ng/L in the all groups exposed. Similarly, it was not observed teratogenic effects in the groups exposed to different concentrations. In the heart frequencies of the embryos, significant variations were observed (p < 0 .05) when compared to the control group. Adults exposed to free andcomplexed finasteride and testosterone had significant alterations (p < 0.05) in the most active behaviors such as slow swim and fast swim, as well as in agonistic behaviors. It can be concluded that in tests with embryos, FIN and FIN-β-CD as well T and T- β-CD, may interfere in the heart rate and the exposure of adults to the same hormones promoted a reduction in their locomotor capacity and can increase in the aggressiveness of the animals.A finasterida e a testosterona são hormônios que possuem ação anti-androgênica (inibição da 5α-redutase) e androgênica respectivamente, que podem agir como disruptores endócrinos causando efeitos negativos aos animais aquáticos e suas proles. A finasterida foi desenvolvida inicialmente para tratar a hiperplasia prostática benigna, porém, foi apresentada como uma grande descoberta no tratamento da Alopecia androgenética (calvície), já a testosterona é o hormônio responsável pelas características sexuais secundárias e tem como metabólito mais ativo a dihidrotestosterona, principal responsável pela calvície. Entretanto, a finasterida assim como a testosterona são comumente usadas em diversas situações, tanto por homens no tratamento contra a perda de cabelo (finasterida) ou na reversão sexual de peixes (testosterona), o que eleva as presenças desses hormônios no ambiente aquático. Uma vez no ambiente tanto a finasterida como a testosterona passam a ter ação de disruptores endócrinos, sendo um dos mais potentes poluentes aquáticos. Por possui propriedades hidrofóbicas que reduz suas biodisponibilidades, tais hormônios podem ser complexados a ciclodextrinas (CDs) o que reduzir esta limitação, logo sua utilização em complexos de inclusão aumentaria sua solubilidade e absorção, podendo alterar uma ou mais vias hormonais. Diante do exposto, o presente trabalho tem como objetivo avaliar os efeitos tóxicos do complexo de inclusão Finasterida β-ciclodextrina (β-CD) e Testosterona β-ciclodextrina (β-CD), através da exposição crônica em concentrações subletais sob os e embriões e adultos de zebrafish (Danio rerio). Os embriões foram expostos por períodos de 96 horas pós-fertilização para a avaliação da mortalidade, efeitos teratogênicos e frequência cardíaca. Os grupos foram divididos em: controle, finasterida (FIN) e testosterona (T) livres nas concentrações de 280, 560, 1120, 2240, 4480 ng/L, e finasterida (FIN-β-CD) e testosterona (T-β-CD) complexadas a β-CD nas concentrações de 280 e 4480 ng/L. Os adultos foram expostos por um período de 60 dias para a avaliar alterações comportamentais e sobrevivência diária relacionadas a exposição de finasterida (FIN) e testosterona (T) livres e complexadas (β-CD) nas concentrações 280 e 4480 ng/L. Os embriões, não apresentaram mortalidade nas concentrações de 280 - 4480 ng/L em todo os todos os grupos expostos. Efeitos teratogênicos, de forma semelhante também não foram observados nos grupos nas diferentes concentrações. Nas frequências cardíacas dos embriões, foram constatadas variações significativas (p <0,05) quando comparadas ao grupo controle em todos os grupos expostos. Os adultos expostos às concentrações de finasterida e testosterona livres e complexadas apresentaram modificações (p < 0,05) na frequência de exibição de comportamentos ativos como Nadar lento e Nadar rápido, como também nos comportamentos agonísticos. Conclui-se então, que em testes com os embriões, FIN e FIN-β-CD assim como T e T- β-CD, podem modificar suas frequências cardíacas e que a exposição dos adultos aos mesmo hormônios promoveram uma redução na sua capacidade locomotora e um aumento na agressividade dos animais.Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-05-16T13:16:47Z No. of bitstreams: 1 Sergio Clementino da Costa.pdf: 1278139 bytes, checksum: 9762e5c57a26ef77c09e890e773a4e28 (MD5)Made available in DSpace on 2018-05-16T13:17:57Z (GMT). No. of bitstreams: 1 Sergio Clementino da Costa.pdf: 1278139 bytes, checksum: 9762e5c57a26ef77c09e890e773a4e28 (MD5) Previous issue date: 2018-02-28application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalToxicidadeFinasteridaTestosteronaCiclodextrinaDanio rerioCIENCIAS AGRARIAS::MEDICINA VETERINARIAEfeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerioinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALSergio Clementino da Costa.pdfSergio Clementino da Costa.pdfapplication/pdf1278139http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7250/2/Sergio+Clementino+da+Costa.pdf9762e5c57a26ef77c09e890e773a4e28MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7250/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/72502018-06-18 11:08:24.715oai:tede2:tede2/7250Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:35:24.240919Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
title |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
spellingShingle |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio COSTA, Sérgio Clementino da Toxicidade Finasterida Testosterona Ciclodextrina Danio rerio CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
title_full |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
title_fullStr |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
title_full_unstemmed |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
title_sort |
Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio |
author |
COSTA, Sérgio Clementino da |
author_facet |
COSTA, Sérgio Clementino da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
CADENA, Pabyton Gonçalves |
dc.contributor.advisor-co1.fl_str_mv |
CADENA, Marilia Ribeiro Sales |
dc.contributor.referee1.fl_str_mv |
AMORIM, Marleyne José Afonso Accioly Lins |
dc.contributor.referee2.fl_str_mv |
BEZERRA, Raquel Pedrosa |
dc.contributor.referee3.fl_str_mv |
SOUZA, Paulo Eleutério de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7895981207021342 |
dc.contributor.author.fl_str_mv |
COSTA, Sérgio Clementino da |
contributor_str_mv |
CADENA, Pabyton Gonçalves CADENA, Marilia Ribeiro Sales AMORIM, Marleyne José Afonso Accioly Lins BEZERRA, Raquel Pedrosa SOUZA, Paulo Eleutério de |
dc.subject.por.fl_str_mv |
Toxicidade Finasterida Testosterona Ciclodextrina Danio rerio |
topic |
Toxicidade Finasterida Testosterona Ciclodextrina Danio rerio CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Finasteride and testosterone are hormones that have anti-androgenic action (inhibition of 5α-reductase) and androgen respectively, which can act as endocrine disruptors causing negative effects on aquatic animals and their offspring. Finasteride was initially developed to treat benign prostatic hyperplasia, however, was presented as a major discovery in the treatment of androgenetic alopecia (baldness) already the testosterone is the hormone responsible for secondary sexual characteristics and has the most active metabolite the dihydrotestosterone, main responsible for baldness. However, finasteride as well as testosterone are commonly used in various situations, both by men in the treatment against hair loss (finasteride) or sexual reversal of fish (testosterone), which increases the presence of these hormones in the aquatic environment. Once in the environment both finasteride and testosterone begin to have action of endocrine disruptors, being one of the most powerful aquatic pollutants. Because it has hydrophobic properties which reduces their bioavailability, such hormones can be complexed to cyclodextrins (CDs) to reduce this limitation, therefore its use in inclusion complexes would increase its solubility and absorption, and may change one or more hormonal pathways. In view of the foregoing, the present study aims to evaluate the toxic effects of the inclusion complex Finasteride β-cyclodextrin (β-CD) and Testosterone β-cyclodextrin (β-CD), through chronic exposure to sublethal concentrations under the embryos and adults of the zebrafish (Danio rerio). The work was aimed to evaluate the toxic effects of finasteride and testosterone free and complexed into cyclodextrin on the physiological and behavioral parameters of embryos and adults of the Danio rerio fish. The embryos were exposed for periods of 96 hours post-fertilization for the evaluation of mortality, teratogenic effects, and heart rate. The groups were divided into control, free finasteride (FIN) and testosterone (T) at the concentrations of 280, 560, 1120, 2240, 4480 ng/L, and finasteride (FIN-β-CD) and testosterone (T-β-CD) complexed into β-CD at concentrations of 280 and 4480 ng/L. Adults were exposed for a period of 60 days to evaluate behavioral changes and daily survival related to exposure to free finasteride (FIN) and testosterone (T) complexed into β-CD at concentrations 280 and 4480 ng/L. The embryos did not exhibit mortality at concentrations of 280 - 4480 ng/L in the all groups exposed. Similarly, it was not observed teratogenic effects in the groups exposed to different concentrations. In the heart frequencies of the embryos, significant variations were observed (p < 0 .05) when compared to the control group. Adults exposed to free andcomplexed finasteride and testosterone had significant alterations (p < 0.05) in the most active behaviors such as slow swim and fast swim, as well as in agonistic behaviors. It can be concluded that in tests with embryos, FIN and FIN-β-CD as well T and T- β-CD, may interfere in the heart rate and the exposure of adults to the same hormones promoted a reduction in their locomotor capacity and can increase in the aggressiveness of the animals. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-05-16T13:17:57Z |
dc.date.issued.fl_str_mv |
2018-02-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Sérgio Clementino da. Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio. 2018. 54 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7250 |
identifier_str_mv |
COSTA, Sérgio Clementino da. Efeitos tóxicos da finasterida e testosterona livres e complexadas a ciclodextrina na fisiologia e comportamento de Danio rerio. 2018. 54 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7250 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-1510757014399315592 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-8922364187987396204 |
dc.relation.cnpq.fl_str_mv |
453670264235017319 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biociência Animal |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Morfologia e Fisiologia Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.source.none.fl_str_mv |
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UFRPE |
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Biblioteca Digital de Teses e Dissertações da UFRPE |
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Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7250/2/Sergio+Clementino+da+Costa.pdf http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7250/1/license.txt |
bitstream.checksum.fl_str_mv |
9762e5c57a26ef77c09e890e773a4e28 bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
_version_ |
1810102247757971456 |