Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil

Detalhes bibliográficos
Autor(a) principal: DOURADO, Joanna
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5686
Resumo: Shrimp farming all over the world has been affected in the last years by many diseases, that have contributed to the development indexes decline in the production of farmed marine shrimp. On top of the losses due to diseases, it's important to mention that some of the vibrio species are zoonotic agents, meaning they can also cause diseases in humans. Given the importance of bacteria of the genus Vibrio to shrimp farming and public health, the aim of this study was to evaluate marine shrimp, correlate the clinical findings with the presence of the vibrio bacteria and identify the isolated species in the water of the ponds of cultivated marine shrimp in Pernambuco. The samples were collected from four coastal farms and the laboratory analysis consisted of the research and identification of the vibrio bacteria in the hepatopancreas and in the water of the tanks, and identification of any alteration by examination of fresh samples. Thiosulphate citrate bile salt agar (TCBS) was used as the culture media and the selected colonies were later biochemically tested. Four hundred and eighty shrimps were analyzed, of hose 424 (88.33%) showed to have vibrio and one or more alterations. There were identified a total of 903 alterations, the most frequent of muscle tissue (329; 36.43%) and the least frequent of the hepatopancreas tubules (121; 13.40%). When analizing the growth cycle, it was observed the largest number of alterations (503; 55.7%) in the dry season. Taking into account the weight of the shrimps, it was observed 540 (59.8%) alterations when the average weight was 8g. Out of all the analyzed animals, 314 (65.42%) showed to have vibrio, being 38 samples from the ponds water and 605 shrimp isolated. The most abundant species was V. mediterranei, (21.31%), considered an environmental species non pathogenic to shrimp, and the least abundant species were V. campbellii, V. rotiferanus, V. shiloni, V. splendidus, V. tapetis and V. wodanis (0.16 %). A dependency between the alterations found on the exam and the presence of the vibrio was noticed, showing that the aforementioned exam, combined to microbiological analyzes, is an important tool that should be used when monitoring the farms. It was also noticed a great variety of pathogenic and non pathogenic species of vibrio in the studied samples. Such microorganisms could, eventually, be related to shrimp and human infections, which could represent a risk for shrimp farming and public health respectively.
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spelling MENDES, Emiko ShinozakiMENDES, Paulo de PaulaSANTOS, Fernando Leandro dosCOIMBRA, Maria Raquel Mourahttp://lattes.cnpq.br/4334267148730417DOURADO, Joanna2016-10-13T12:20:42Z2009-02-13DOURADO, Joanna. Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil. 2009. 48 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5686Shrimp farming all over the world has been affected in the last years by many diseases, that have contributed to the development indexes decline in the production of farmed marine shrimp. On top of the losses due to diseases, it's important to mention that some of the vibrio species are zoonotic agents, meaning they can also cause diseases in humans. Given the importance of bacteria of the genus Vibrio to shrimp farming and public health, the aim of this study was to evaluate marine shrimp, correlate the clinical findings with the presence of the vibrio bacteria and identify the isolated species in the water of the ponds of cultivated marine shrimp in Pernambuco. The samples were collected from four coastal farms and the laboratory analysis consisted of the research and identification of the vibrio bacteria in the hepatopancreas and in the water of the tanks, and identification of any alteration by examination of fresh samples. Thiosulphate citrate bile salt agar (TCBS) was used as the culture media and the selected colonies were later biochemically tested. Four hundred and eighty shrimps were analyzed, of hose 424 (88.33%) showed to have vibrio and one or more alterations. There were identified a total of 903 alterations, the most frequent of muscle tissue (329; 36.43%) and the least frequent of the hepatopancreas tubules (121; 13.40%). When analizing the growth cycle, it was observed the largest number of alterations (503; 55.7%) in the dry season. Taking into account the weight of the shrimps, it was observed 540 (59.8%) alterations when the average weight was 8g. Out of all the analyzed animals, 314 (65.42%) showed to have vibrio, being 38 samples from the ponds water and 605 shrimp isolated. The most abundant species was V. mediterranei, (21.31%), considered an environmental species non pathogenic to shrimp, and the least abundant species were V. campbellii, V. rotiferanus, V. shiloni, V. splendidus, V. tapetis and V. wodanis (0.16 %). A dependency between the alterations found on the exam and the presence of the vibrio was noticed, showing that the aforementioned exam, combined to microbiological analyzes, is an important tool that should be used when monitoring the farms. It was also noticed a great variety of pathogenic and non pathogenic species of vibrio in the studied samples. Such microorganisms could, eventually, be related to shrimp and human infections, which could represent a risk for shrimp farming and public health respectively.A carcinicultura mundial tem enfrentado nos últimos anos vários impactos causados por enfermidades, que contribuíram para a queda dos índices de desenvolvimento na produção de camarão marinho cultivado. Além das perdas causadas devido às enfermidades, ressalta-se que algumas espécies de víbrio são agentes zoonóticos, ou seja, podem causar doenças também em humanos. Dada a importância das bactérias do gênero Vibrio para a carcinicultura e saúde pública, objetivou-se avaliar camarões marinhos, associar os achados clínicos com a presença de bactérias do gênero Vibrio e identificar as espécies isoladas da água dos viveiros e de camarões marinhos (Litopenaeus vannamei) cultivados no litoral do estado de Pernambuco. As amostras foram coletadas em quatro carciniculturas do litoral e as análises laboratoriais compreenderam pesquisa e identificação de bactérias do gênero Víbrio no hepatopâncreas e na água dos viveiros, e identificação de alterações através do exame a fresco. Para o isolamento das espécies foi utilizado o meio de cultivo Ágar Tiossulfato Citrato Sais de Bile Sacarose (TCBS) e as colônias selecionadas foram posteriormente submetidas a provas bioquímicas. Foram analisados no total 480 camarões, destes 424 (88,33%) apresentaram um ou mais tipos de alteração. Foi identificado um total de 903 alterações, sendo a mais freqüente a da musculatura (329; 36,43%) e a menos freqüente a alteração dos túbulos do hepatopâncreas (121; 13,40%). Com relação ao ciclo de cultivo, foi observado o maior número de alterações (503; 55,7%) no período de estio. Levando-se em consideração a média de peso dos camarões verificou-se 540 (59,8%) alterações quando os mesmos estavam com peso médio de 8 g. Do total de animais analisados 314 (65,42%) apresentavam vibrio e uma ou mais alteração no exame a fresco. Foi obtido um total de 643 isolados de Vibrio, sendo 38 de amostras de água dos viveiros e 605 isolados de camarão. A espécie mais abundante foi V. mediterranei (21,31%), considerada espécie ambiental e não patogênica para o camarão, e as espécies de menor predominância foram V. campbellii, V. rotiferianus, V. shiloni, V. splendidus, V. tapetis e V. wodanis (0,16%). Foi verificada uma dependência entre as alterações encontradas no exame a fresco e a presença de vibrio, concluindo-se que o referido exame, associado à análise microbiológica é uma importante ferramenta que deve ser utilizada no monitoramento da saúde dos animais. Verificou-se também uma ampla variedade de espécies patogênicas e não patogênicas de víbrio nas amostras estudadas, que podem eventualmente estar relacionados a infecções nos camarões e nos humanos, representando um risco para a carcinicultura e para a saúde pública respectivamente.Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T12:20:42Z No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5)Made available in DSpace on 2016-10-13T12:20:42Z (GMT). No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Previous issue date: 2009-02-13application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência VeterináriaUFRPEBrasilDepartamento de Medicina VeterináriaCamarão marinhoDoençaVibrioseHepatopâncreaShrimp farmsDiseaseVibriosisCIENCIAS AGRARIAS::MEDICINA VETERINARIAVibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3061482854177903105600600600-3020210563763616780453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5686/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALJoanna Dourado.pdfJoanna Dourado.pdfapplication/pdf721470http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5686/2/Joanna+Dourado.pdf1ade18b6e4af854fcb0abb1162f7c2bbMD52tede2/56862016-10-13 09:20:42.708oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:33:23.500167Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
title Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
spellingShingle Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
DOURADO, Joanna
Camarão marinho
Doença
Vibriose
Hepatopâncrea
Shrimp farms
Disease
Vibriosis
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
title_full Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
title_fullStr Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
title_full_unstemmed Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
title_sort Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil
author DOURADO, Joanna
author_facet DOURADO, Joanna
author_role author
dc.contributor.advisor1.fl_str_mv MENDES, Emiko Shinozaki
dc.contributor.advisor-co1.fl_str_mv MENDES, Paulo de Paula
dc.contributor.referee1.fl_str_mv SANTOS, Fernando Leandro dos
dc.contributor.referee2.fl_str_mv COIMBRA, Maria Raquel Moura
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4334267148730417
dc.contributor.author.fl_str_mv DOURADO, Joanna
contributor_str_mv MENDES, Emiko Shinozaki
MENDES, Paulo de Paula
SANTOS, Fernando Leandro dos
COIMBRA, Maria Raquel Moura
dc.subject.por.fl_str_mv Camarão marinho
Doença
Vibriose
Hepatopâncrea
topic Camarão marinho
Doença
Vibriose
Hepatopâncrea
Shrimp farms
Disease
Vibriosis
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv Shrimp farms
Disease
Vibriosis
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description Shrimp farming all over the world has been affected in the last years by many diseases, that have contributed to the development indexes decline in the production of farmed marine shrimp. On top of the losses due to diseases, it's important to mention that some of the vibrio species are zoonotic agents, meaning they can also cause diseases in humans. Given the importance of bacteria of the genus Vibrio to shrimp farming and public health, the aim of this study was to evaluate marine shrimp, correlate the clinical findings with the presence of the vibrio bacteria and identify the isolated species in the water of the ponds of cultivated marine shrimp in Pernambuco. The samples were collected from four coastal farms and the laboratory analysis consisted of the research and identification of the vibrio bacteria in the hepatopancreas and in the water of the tanks, and identification of any alteration by examination of fresh samples. Thiosulphate citrate bile salt agar (TCBS) was used as the culture media and the selected colonies were later biochemically tested. Four hundred and eighty shrimps were analyzed, of hose 424 (88.33%) showed to have vibrio and one or more alterations. There were identified a total of 903 alterations, the most frequent of muscle tissue (329; 36.43%) and the least frequent of the hepatopancreas tubules (121; 13.40%). When analizing the growth cycle, it was observed the largest number of alterations (503; 55.7%) in the dry season. Taking into account the weight of the shrimps, it was observed 540 (59.8%) alterations when the average weight was 8g. Out of all the analyzed animals, 314 (65.42%) showed to have vibrio, being 38 samples from the ponds water and 605 shrimp isolated. The most abundant species was V. mediterranei, (21.31%), considered an environmental species non pathogenic to shrimp, and the least abundant species were V. campbellii, V. rotiferanus, V. shiloni, V. splendidus, V. tapetis and V. wodanis (0.16 %). A dependency between the alterations found on the exam and the presence of the vibrio was noticed, showing that the aforementioned exam, combined to microbiological analyzes, is an important tool that should be used when monitoring the farms. It was also noticed a great variety of pathogenic and non pathogenic species of vibrio in the studied samples. Such microorganisms could, eventually, be related to shrimp and human infections, which could represent a risk for shrimp farming and public health respectively.
publishDate 2009
dc.date.issued.fl_str_mv 2009-02-13
dc.date.accessioned.fl_str_mv 2016-10-13T12:20:42Z
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dc.identifier.citation.fl_str_mv DOURADO, Joanna. Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil. 2009. 48 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5686
identifier_str_mv DOURADO, Joanna. Vibriose em camarões marinhos ( Litopenaeus vannamei, Boone 1931) cultivados no litoral de Pernambuco, Brasil. 2009. 48 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5686
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência Veterinária
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Medicina Veterinária
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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