Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9558 |
Resumo: | Microalgae are extremely important for the functioning of aquatic ecosystems, being mainly unicellular organisms and producers of photosynthetic pigments. As a cosmopolitan group, it is present in several ecosystems, where it helps in diverse ecosystem processes, from the maintenance of the base of the trophic web to the cycling of nutrients. They have applications in aquaculture covering from the nutrition of young organisms of species of commercial interest to the bioremediation of effluents produced. Its cultivation produces important biomolecules for nutritional, pharmacological, and biochemical use, as well as lipids for the production of biodiesel. Among the groups of microalgae, the dinoflagellates stand out for their expressive production of biomolecules of interest. However, they present cultivation difficulties related to the lack of detailed information about their growth and nutritional characteristics. Thus, the lack of information on the main cultivation variables (i.e., light, nutrient concentration, shear stress, etc.), complicates the biomass production of this promising microalgae group. The present dissertation aims to evaluate the growth performance and the biochemical composition, with emphasis on protein content, of the dinoflagellate Symbiodinium glynnii grown at different nitrogen-to-phosphorus ratios. Four treatments were used: Zero Nitrogen (0:1), Medium Nitrogen (7:1), Normal Nitrogen (14:1) - as control - and Double Nitrogen (28:1), obtained by modifying the NaNO3 solution of the f/2 medium. Three successive subcultures were performed in triplicate. Cultures were conducted in 250mL Erlenmeyer flasks, to which seawater (30 PSU) and B-complex vitamins were added. S. glynnii cells were inoculated at an initial concentration of 5 x 104 cells mL-1. These were maintained at 22 ± 1 °C, under constant aeration, the irradiance of 150 μmol photons m-2 s-1, and integral photoperiod. Growth was monitored using a Neubauer haematocytometer (72h), and the parameters DCM, K, μ, and TD were calculated. A photocolorimeter was used to analyse weekly N and P concentrations. The third sub cultivation was performed to obtain the production and yield of dry biomass, being submitted at the end to centrifugation, freeze-drying, and weighing. Dried biomass was subjected to determination of total protein (micro- Kjeldahl) and crude lipid content (AOCS Am 5-04). ZN did not show cellular increment in the second sub cultivation. S. glynnii show higher DCM when cultivated in MN (87.17 ± 8.46 x 104 cells mL-1), which was significantly different from treatments DN and NN. Higher K was verified in DN treatment (0.19 ± 0.02 div. day-1). For μ there were no significant differences between treatments (p > 0.05). Lower TD in DN treatment (5.26 ± 0.38 days). The highest dry biomass yield found in DN was 753.33 ± 14.14 mg L-1, as well as daily productivity (31.39 ± 0.59 mg L-1 day-1). Growth curves showed consistent growth, as well as the N:P uptake, showed the expected decay in MN, NN, and DN. The best growth variables were obtained when higher concentrations of N in the N:P ratio (DN - 28:1) were used, combined with higher yields of biomass and total protein content (31.51 ± 0.15 %). N concentrations in the N:P ratios between 14:1 (NN) and 28:1 (DN) is recommended for the cultivation of Symbiodinium glynnii. Further studies are needed to understand the mechanisms that regulate the metabolism of these dinoflagellates. |
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OLIVERA GÁLVEZ, AlfredoABREU, Jéssika Lima deSANTOS, Juliana Ferreira doshttp://lattes.cnpq.br/4224003240625541OLIVEIRA, Deyvid Willame Silva2024-03-14T12:40:31Z2022-02-18OLIVEIRA, Deyvid Willame Silva. Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura. 2022. 45 f. Dissertação (Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9558Microalgae are extremely important for the functioning of aquatic ecosystems, being mainly unicellular organisms and producers of photosynthetic pigments. As a cosmopolitan group, it is present in several ecosystems, where it helps in diverse ecosystem processes, from the maintenance of the base of the trophic web to the cycling of nutrients. They have applications in aquaculture covering from the nutrition of young organisms of species of commercial interest to the bioremediation of effluents produced. Its cultivation produces important biomolecules for nutritional, pharmacological, and biochemical use, as well as lipids for the production of biodiesel. Among the groups of microalgae, the dinoflagellates stand out for their expressive production of biomolecules of interest. However, they present cultivation difficulties related to the lack of detailed information about their growth and nutritional characteristics. Thus, the lack of information on the main cultivation variables (i.e., light, nutrient concentration, shear stress, etc.), complicates the biomass production of this promising microalgae group. The present dissertation aims to evaluate the growth performance and the biochemical composition, with emphasis on protein content, of the dinoflagellate Symbiodinium glynnii grown at different nitrogen-to-phosphorus ratios. Four treatments were used: Zero Nitrogen (0:1), Medium Nitrogen (7:1), Normal Nitrogen (14:1) - as control - and Double Nitrogen (28:1), obtained by modifying the NaNO3 solution of the f/2 medium. Three successive subcultures were performed in triplicate. Cultures were conducted in 250mL Erlenmeyer flasks, to which seawater (30 PSU) and B-complex vitamins were added. S. glynnii cells were inoculated at an initial concentration of 5 x 104 cells mL-1. These were maintained at 22 ± 1 °C, under constant aeration, the irradiance of 150 μmol photons m-2 s-1, and integral photoperiod. Growth was monitored using a Neubauer haematocytometer (72h), and the parameters DCM, K, μ, and TD were calculated. A photocolorimeter was used to analyse weekly N and P concentrations. The third sub cultivation was performed to obtain the production and yield of dry biomass, being submitted at the end to centrifugation, freeze-drying, and weighing. Dried biomass was subjected to determination of total protein (micro- Kjeldahl) and crude lipid content (AOCS Am 5-04). ZN did not show cellular increment in the second sub cultivation. S. glynnii show higher DCM when cultivated in MN (87.17 ± 8.46 x 104 cells mL-1), which was significantly different from treatments DN and NN. Higher K was verified in DN treatment (0.19 ± 0.02 div. day-1). For μ there were no significant differences between treatments (p > 0.05). Lower TD in DN treatment (5.26 ± 0.38 days). The highest dry biomass yield found in DN was 753.33 ± 14.14 mg L-1, as well as daily productivity (31.39 ± 0.59 mg L-1 day-1). Growth curves showed consistent growth, as well as the N:P uptake, showed the expected decay in MN, NN, and DN. The best growth variables were obtained when higher concentrations of N in the N:P ratio (DN - 28:1) were used, combined with higher yields of biomass and total protein content (31.51 ± 0.15 %). N concentrations in the N:P ratios between 14:1 (NN) and 28:1 (DN) is recommended for the cultivation of Symbiodinium glynnii. Further studies are needed to understand the mechanisms that regulate the metabolism of these dinoflagellates.As microalgas possuem grande importância ao funcionamento dos ecossistemas aquáticos, são organismos predominantemente unicelulares e possuem pigmentos fotossintetizantes. Por ser um grupo cosmopolita, está presente em diversos ecossistemas, onde nestes, auxilia em diversos processos ecossistêmicos, desde a manutenção da base da teia trófica até a ciclagem de nutrientes do meio. Permitem aplicações na aquicultura, desde a nutrição de organismos jovens das espécies de interesse comercial a biorremediação dos efluentes produzidos. Seu cultivo também permite a obtenção de importantes biomoléculas de uso nutricional, farmacológico e bioquímico, assim como de lipídios para a produção de biodiesel. Dentre os diferentes grupos de microalgas, os dinoflagelados se destacam por sua expressiva produção de biomoléculas, entretanto estes apresentam dificuldades de cultivo relacionadas à falta de informações detalhadas sobre suas características de crescimento e de nutrição. Assim, a falta de informações sobre as principais variáveis de cultivo (i.e., luz, concentração de nutrientes, tensões de cisalhamento etc.), dificultam a produção de biomassa deste promissor grupo de microalgas. A presente dissertação tem como objetivo avaliar o desempenho de crescimento e a composição bioquímica, com ênfase no teor de proteínas, do dinoflagelado Symbiodinium glynnii cultivado em diferentes proporções nitrogênio:fósforo. Foram utilizados quatro tratamentos, Zero Nitrogênio (0:1), Meio Nitrogênio (7:1), Normal Nitrogênio (14:1) e Dobro Nitrogênio (28:1), obtidos por meio da modificação da solução de NaNO3 do meio f/2. Foram realizados três subcultivos sucessivos em triplicata. Os cultivos foram conduzidos em erlenmeyers de 250mL, aos quais foram adicionados água do mar (30 UPS) e vitaminas do complexo B. Foram inoculadas células de S. glynnii a uma concentração inicial de 5 x 104 cél. mL-1. As culturas foram mantidas em 22 ± 1 °C, sob aeração constante, irradiância de 150 μmol fótons m-2 s-1 e fotoperíodo integral. O crescimento foi acompanhado por meio de hematocitômetro de Neubauer (72h), sendo calculadas as variáveis DCM, K, μ e TD. Um fotocolorímetro foi utilizado para avaliar as concentrações semanais de N e P. O terceiro subcultivo foi executado para obtenção da produção e produtividade em biomassa seca, sendo submetido ao término a centrifugação, liofilização e pesagem. A biomassa seca foi submetida a determinação do conteúdo proteico e teor de lipídios brutos pelos métodos micro Kjeldahl e AOCS Am 5-04, respectivamente. ZN não apresentou incremento celular no segundo subcultivo. S. glynnii apresentou maior DCM quando cultivado em MN (87,17 ± 8,46 x 104 cél. mL-1), sendo este significativamente diferente dos tratamentos DN e NN. Maior K foi verificado no tratamento DN (0,19 ± 0,02 div. dia-1). Para μ não houveram diferenças significativas entre os tratamentos (p > 0,05). Menor TD no tratamento DN (5,26 ± 0,38 dias). Maior rendimento em biomassa seca encontrado no tratamento DN com 753,33 ± 14,14 mg L-1, assim como produtividade diária (31,39 ± 0,59 mg L-1 dia-1). As curvas de crescimento observadas apresentaram crescimento consistente, assim como o consumo N:P apresentou o decaimento esperado nos tratamentos MN, NN e DN. As melhores variáveis de crescimento foram obtidas quando utilizadas maiores concentrações de N na relação N:P (DN - 28:1), atrelada a maiores rendimentos em biomassa e proteínas (31,51 ± 0,15 %). São recomendados para o cultivo do Symbiodinium glynnii concentrações de N nas proporções N:P entre 14:1 (NN) e 28:1 (DN). Mais estudos são necessários para entender os mecanismos que regulam o metabolismo destes dinoflagelados.Submitted by (ana.araujo@ufrpe.br) on 2024-03-14T12:40:31Z No. of bitstreams: 1 Deyvid Willame Silva Oliveira.pdf: 588510 bytes, checksum: 2b02ae323b2094defa3b50346861ca62 (MD5)Made available in DSpace on 2024-03-14T12:40:31Z (GMT). No. of bitstreams: 1 Deyvid Willame Silva Oliveira.pdf: 588510 bytes, checksum: 2b02ae323b2094defa3b50346861ca62 (MD5) Previous issue date: 2022-02-18Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Recursos Pesqueiros e AquiculturaUFRPEBrasilDepartamento de Pesca e AquiculturaMicroalgaDinoflageladoCrescimentoBiomassaProteínaMeios de culturaNitrogênioCIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCACultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de culturainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis80217415640343225476006006006007231936942857037408-61317501987095198112075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALDeyvid Willame Silva Oliveira.pdfDeyvid Willame Silva Oliveira.pdfapplication/pdf588510http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9558/2/Deyvid+Willame+Silva+Oliveira.pdf2b02ae323b2094defa3b50346861ca62MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9558/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/95582024-03-14 09:40:31.429oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-03-14T12:40:31Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
title |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
spellingShingle |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura OLIVEIRA, Deyvid Willame Silva Microalga Dinoflagelado Crescimento Biomassa Proteína Meios de cultura Nitrogênio CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
title_short |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
title_full |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
title_fullStr |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
title_full_unstemmed |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
title_sort |
Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura |
author |
OLIVEIRA, Deyvid Willame Silva |
author_facet |
OLIVEIRA, Deyvid Willame Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
OLIVERA GÁLVEZ, Alfredo |
dc.contributor.referee1.fl_str_mv |
ABREU, Jéssika Lima de |
dc.contributor.referee2.fl_str_mv |
SANTOS, Juliana Ferreira dos |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4224003240625541 |
dc.contributor.author.fl_str_mv |
OLIVEIRA, Deyvid Willame Silva |
contributor_str_mv |
OLIVERA GÁLVEZ, Alfredo ABREU, Jéssika Lima de SANTOS, Juliana Ferreira dos |
dc.subject.por.fl_str_mv |
Microalga Dinoflagelado Crescimento Biomassa Proteína Meios de cultura Nitrogênio |
topic |
Microalga Dinoflagelado Crescimento Biomassa Proteína Meios de cultura Nitrogênio CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
description |
Microalgae are extremely important for the functioning of aquatic ecosystems, being mainly unicellular organisms and producers of photosynthetic pigments. As a cosmopolitan group, it is present in several ecosystems, where it helps in diverse ecosystem processes, from the maintenance of the base of the trophic web to the cycling of nutrients. They have applications in aquaculture covering from the nutrition of young organisms of species of commercial interest to the bioremediation of effluents produced. Its cultivation produces important biomolecules for nutritional, pharmacological, and biochemical use, as well as lipids for the production of biodiesel. Among the groups of microalgae, the dinoflagellates stand out for their expressive production of biomolecules of interest. However, they present cultivation difficulties related to the lack of detailed information about their growth and nutritional characteristics. Thus, the lack of information on the main cultivation variables (i.e., light, nutrient concentration, shear stress, etc.), complicates the biomass production of this promising microalgae group. The present dissertation aims to evaluate the growth performance and the biochemical composition, with emphasis on protein content, of the dinoflagellate Symbiodinium glynnii grown at different nitrogen-to-phosphorus ratios. Four treatments were used: Zero Nitrogen (0:1), Medium Nitrogen (7:1), Normal Nitrogen (14:1) - as control - and Double Nitrogen (28:1), obtained by modifying the NaNO3 solution of the f/2 medium. Three successive subcultures were performed in triplicate. Cultures were conducted in 250mL Erlenmeyer flasks, to which seawater (30 PSU) and B-complex vitamins were added. S. glynnii cells were inoculated at an initial concentration of 5 x 104 cells mL-1. These were maintained at 22 ± 1 °C, under constant aeration, the irradiance of 150 μmol photons m-2 s-1, and integral photoperiod. Growth was monitored using a Neubauer haematocytometer (72h), and the parameters DCM, K, μ, and TD were calculated. A photocolorimeter was used to analyse weekly N and P concentrations. The third sub cultivation was performed to obtain the production and yield of dry biomass, being submitted at the end to centrifugation, freeze-drying, and weighing. Dried biomass was subjected to determination of total protein (micro- Kjeldahl) and crude lipid content (AOCS Am 5-04). ZN did not show cellular increment in the second sub cultivation. S. glynnii show higher DCM when cultivated in MN (87.17 ± 8.46 x 104 cells mL-1), which was significantly different from treatments DN and NN. Higher K was verified in DN treatment (0.19 ± 0.02 div. day-1). For μ there were no significant differences between treatments (p > 0.05). Lower TD in DN treatment (5.26 ± 0.38 days). The highest dry biomass yield found in DN was 753.33 ± 14.14 mg L-1, as well as daily productivity (31.39 ± 0.59 mg L-1 day-1). Growth curves showed consistent growth, as well as the N:P uptake, showed the expected decay in MN, NN, and DN. The best growth variables were obtained when higher concentrations of N in the N:P ratio (DN - 28:1) were used, combined with higher yields of biomass and total protein content (31.51 ± 0.15 %). N concentrations in the N:P ratios between 14:1 (NN) and 28:1 (DN) is recommended for the cultivation of Symbiodinium glynnii. Further studies are needed to understand the mechanisms that regulate the metabolism of these dinoflagellates. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-02-18 |
dc.date.accessioned.fl_str_mv |
2024-03-14T12:40:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Deyvid Willame Silva. Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura. 2022. 45 f. Dissertação (Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9558 |
identifier_str_mv |
OLIVEIRA, Deyvid Willame Silva. Cultivo do dinoflagelado Symbiodinium glynnii em diferentes meios de cultura. 2022. 45 f. Dissertação (Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9558 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
8021741564034322547 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
7231936942857037408 |
dc.relation.cnpq.fl_str_mv |
-6131750198709519811 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Recursos Pesqueiros e Aquicultura |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Pesca e Aquicultura |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRPE instname:Universidade Federal Rural de Pernambuco (UFRPE) instacron:UFRPE |
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Universidade Federal Rural de Pernambuco (UFRPE) |
instacron_str |
UFRPE |
institution |
UFRPE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
collection |
Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9558/2/Deyvid+Willame+Silva+Oliveira.pdf http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9558/1/license.txt |
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MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
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1800311512578916352 |