Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USC |
Texto Completo: | https://tede2.usc.br:8443/handle/tede/484 |
Resumo: | Leprosy is a chronic infectious disease characterized by changes in tactile, thermal and painful skin sensitivity, due to peripheral nerves involvement. In Brazil is a still endemic disease; fighting it is priority in public health, however, one of the biggest obstacles is the diagnosis. The main route of transmission is done directly, most likely the respiratory tract, requiring a predisposition to acquire the disease. Mycobacterium leprae (M. leprae) is the main causative agent of leprosy and sensitive methods are needed to detect the bactéria. One of the tests of high sensitivity and specificity, the polymerase chain reaction in real time (qPCR) has proved to be a quick, easy and reliable method for molecular detection and quantification of mycobacteria. The aim of this study was to molecularly identify the presence of M. leprae in the tissue of the gingival sulcus and in the saliva, of leprosy, and healthy volunteers. For this, the methodology used was qPCR, in the target gene RLEP (gene repetitive elemento), in DNA samples of M. leprae extracted from the gingival sulcus and saliva. 31 patients with leprosy (GH) and 51 volunteers in the control group (GC) participated in this study. The results were stored in specific and spreadsheets, involving absolute and relative frequencies. It was found that M. leprae was present in saliva and in GH tissue. The qPCR of saliva and positive tissue results from samples with multibacillary characteristics (88.89%), of the borderline lepromatous type (BL) (55.56%), in a reactional state of type 2 (81.5%). The results support the fact that the buccal region may be a site of importance in the early detection and complementary diagnosis of M. leprae. |
id |
USC_03b919d4872cfb6487b88f0edcc165a9 |
---|---|
oai_identifier_str |
oai:localhost:tede/484 |
network_acronym_str |
USC |
network_name_str |
Biblioteca Digital de Teses e Dissertações da USC |
repository_id_str |
|
spelling |
Marta, Sara Nader04042632831http://lattes.cnpq.br/4484420730361244Virmond, Marcos da Cunha Lopeshttp://lattes.cnpq.br/849406415269518836118730806http://lattes.cnpq.br/2934268490810888Trize, Débora De Melo2019-12-06T17:43:40Z2019-08-12Trize, Débora De Melo. Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase. 2019. Tese( Biologia Oral) - Universidade do Sagrado Coração, Bauru, 2019.https://tede2.usc.br:8443/handle/tede/484Leprosy is a chronic infectious disease characterized by changes in tactile, thermal and painful skin sensitivity, due to peripheral nerves involvement. In Brazil is a still endemic disease; fighting it is priority in public health, however, one of the biggest obstacles is the diagnosis. The main route of transmission is done directly, most likely the respiratory tract, requiring a predisposition to acquire the disease. Mycobacterium leprae (M. leprae) is the main causative agent of leprosy and sensitive methods are needed to detect the bactéria. One of the tests of high sensitivity and specificity, the polymerase chain reaction in real time (qPCR) has proved to be a quick, easy and reliable method for molecular detection and quantification of mycobacteria. The aim of this study was to molecularly identify the presence of M. leprae in the tissue of the gingival sulcus and in the saliva, of leprosy, and healthy volunteers. For this, the methodology used was qPCR, in the target gene RLEP (gene repetitive elemento), in DNA samples of M. leprae extracted from the gingival sulcus and saliva. 31 patients with leprosy (GH) and 51 volunteers in the control group (GC) participated in this study. The results were stored in specific and spreadsheets, involving absolute and relative frequencies. It was found that M. leprae was present in saliva and in GH tissue. The qPCR of saliva and positive tissue results from samples with multibacillary characteristics (88.89%), of the borderline lepromatous type (BL) (55.56%), in a reactional state of type 2 (81.5%). The results support the fact that the buccal region may be a site of importance in the early detection and complementary diagnosis of M. leprae.A hanseníase é uma doença infecciosa crônica caracterizada por alterações de sensibilidade tátil, térmica e dolorosa na pele devido ao acometimento dos nervos periféricos. No Brasil é uma enfermidade ainda endêmica, combatê-la é prioridade na saúde pública, entretanto, um dos maiores entraves é sem dúvida o diagnóstico. A principal via de transmissão se faz de forma direta, sendo a mais provável o trato respiratório, necessitando predisposição para adquirir a doença e ter contato íntimo e prolongado com o doente sem tratamento. O Mycobacterium leprae (M. leprae) é o principal agente causador da hanseníase e pode estar presente na mucosa bucal sem causar alteração morfológica, e somente métodos laboratoriais mais sensíveis detectam sua presença. Dentre os exames de alta sensibilidade e especificidade, a reação em cadeia da polimerase em tempo real (qPCR) já provou ser um método rápido, fácil e confiável para a detecção molecular da micobactéria. O objetivo deste estudo foi identificar molecularmente a presença de M. leprae no tecido do sulco gengival e na saliva, em casos de hanseníase, e voluntários ditos saudáveis. Para tal, a metodologia utilizada foi a qPCR, no gene alvo RLEP (elemento genético repetitivo), em amostras de DNA do M. leprae extraídas do sulco gengival e da saliva. Participaram deste estudo 31 pacientes com hanseníase (GH) e 51 voluntários no grupo controle (GC). Constatou-se que há presença do M. leprae na saliva e no tecido do GH. A qPCR de saliva e tecido positivos em GH, resultam das amostras com características multibacilares (88,89%), do tipo borderline lepromatosa (BL) (55,56%), em estado reacional do tipo 2 (81,5%). Os resultados sustentam o fato que a região bucal possa ser um sítio de importância na detecção precoce e no diagnóstico complementar do M. leprae.Submitted by Lidyane Lima (lidyane.lima@usc.br) on 2019-12-06T17:43:40Z No. of bitstreams: 1 Deteccao molecular de Mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseniase (229309).pdf: 1459087 bytes, checksum: bb2e9912251f8a8a2c2efecae38862af (MD5)Made available in DSpace on 2019-12-06T17:43:40Z (GMT). No. of bitstreams: 1 Deteccao molecular de Mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseniase (229309).pdf: 1459087 bytes, checksum: bb2e9912251f8a8a2c2efecae38862af (MD5) Previous issue date: 2019-08-12Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade do Sagrado CoraçãoBiologia OralUSCBrasilCiências da Saúde e BiológicasHanseníaseMucosa BucalDiagnósticoqPCRDoenças negligenciadasLeprosy.Mouth mucosaDiagnosis.Neglected Diseases.CIENCIAS DA SAUDE::ODONTOLOGIADetecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníaseMolecular detection of mycobacterium leprae in gingival sulcus tissue and saliva in leprosy casesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8043334104093724546600600600600-753428887677182963-20704984698792443492075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da USCinstname:Universidade do Sagrado Coração (USC)instacron:USCORIGINALDeteccao molecular de Mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseniase (229309).pdfDeteccao molecular de Mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseniase (229309).pdfapplication/pdf1459087http://localhost:8080/tede/bitstream/tede/484/2/Deteccao+molecular+de+Mycobacterium+leprae+no+tecido+do+sulco+gengival+e+na+saliva+em+casos+de+hanseniase+%28229309%29.pdfbb2e9912251f8a8a2c2efecae38862afMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/484/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/4842019-12-06 15:43:40.915oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.usc.br:8443/http://tede2.usc.br:8080/oai/requestbiblicorjesu@unisagrado.edu.br||normalizacao@usc.bropendoar:2019-12-06T17:43:40Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)false |
dc.title.eng.fl_str_mv |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
dc.title.alternative.eng.fl_str_mv |
Molecular detection of mycobacterium leprae in gingival sulcus tissue and saliva in leprosy cases |
title |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
spellingShingle |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase Trize, Débora De Melo Hanseníase Mucosa Bucal Diagnóstico qPCR Doenças negligenciadas Leprosy. Mouth mucosa Diagnosis. Neglected Diseases. CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
title_full |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
title_fullStr |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
title_full_unstemmed |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
title_sort |
Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase |
author |
Trize, Débora De Melo |
author_facet |
Trize, Débora De Melo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Marta, Sara Nader |
dc.contributor.advisor1ID.fl_str_mv |
04042632831 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4484420730361244 |
dc.contributor.advisor-co1.fl_str_mv |
Virmond, Marcos da Cunha Lopes |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8494064152695188 |
dc.contributor.authorID.fl_str_mv |
36118730806 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2934268490810888 |
dc.contributor.author.fl_str_mv |
Trize, Débora De Melo |
contributor_str_mv |
Marta, Sara Nader Virmond, Marcos da Cunha Lopes |
dc.subject.por.fl_str_mv |
Hanseníase Mucosa Bucal Diagnóstico qPCR Doenças negligenciadas |
topic |
Hanseníase Mucosa Bucal Diagnóstico qPCR Doenças negligenciadas Leprosy. Mouth mucosa Diagnosis. Neglected Diseases. CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Leprosy. Mouth mucosa Diagnosis. Neglected Diseases. |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Leprosy is a chronic infectious disease characterized by changes in tactile, thermal and painful skin sensitivity, due to peripheral nerves involvement. In Brazil is a still endemic disease; fighting it is priority in public health, however, one of the biggest obstacles is the diagnosis. The main route of transmission is done directly, most likely the respiratory tract, requiring a predisposition to acquire the disease. Mycobacterium leprae (M. leprae) is the main causative agent of leprosy and sensitive methods are needed to detect the bactéria. One of the tests of high sensitivity and specificity, the polymerase chain reaction in real time (qPCR) has proved to be a quick, easy and reliable method for molecular detection and quantification of mycobacteria. The aim of this study was to molecularly identify the presence of M. leprae in the tissue of the gingival sulcus and in the saliva, of leprosy, and healthy volunteers. For this, the methodology used was qPCR, in the target gene RLEP (gene repetitive elemento), in DNA samples of M. leprae extracted from the gingival sulcus and saliva. 31 patients with leprosy (GH) and 51 volunteers in the control group (GC) participated in this study. The results were stored in specific and spreadsheets, involving absolute and relative frequencies. It was found that M. leprae was present in saliva and in GH tissue. The qPCR of saliva and positive tissue results from samples with multibacillary characteristics (88.89%), of the borderline lepromatous type (BL) (55.56%), in a reactional state of type 2 (81.5%). The results support the fact that the buccal region may be a site of importance in the early detection and complementary diagnosis of M. leprae. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-12-06T17:43:40Z |
dc.date.issued.fl_str_mv |
2019-08-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Trize, Débora De Melo. Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase. 2019. Tese( Biologia Oral) - Universidade do Sagrado Coração, Bauru, 2019. |
dc.identifier.uri.fl_str_mv |
https://tede2.usc.br:8443/handle/tede/484 |
identifier_str_mv |
Trize, Débora De Melo. Detecção molecular de mycobacterium leprae no tecido do sulco gengival e na saliva em casos de hanseníase. 2019. Tese( Biologia Oral) - Universidade do Sagrado Coração, Bauru, 2019. |
url |
https://tede2.usc.br:8443/handle/tede/484 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
8043334104093724546 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-753428887677182963 |
dc.relation.cnpq.fl_str_mv |
-2070498469879244349 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Sagrado Coração |
dc.publisher.program.fl_str_mv |
Biologia Oral |
dc.publisher.initials.fl_str_mv |
USC |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciências da Saúde e Biológicas |
publisher.none.fl_str_mv |
Universidade do Sagrado Coração |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USC instname:Universidade do Sagrado Coração (USC) instacron:USC |
instname_str |
Universidade do Sagrado Coração (USC) |
instacron_str |
USC |
institution |
USC |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USC |
collection |
Biblioteca Digital de Teses e Dissertações da USC |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/484/2/Deteccao+molecular+de+Mycobacterium+leprae+no+tecido+do+sulco+gengival+e+na+saliva+em+casos+de+hanseniase+%28229309%29.pdf http://localhost:8080/tede/bitstream/tede/484/1/license.txt |
bitstream.checksum.fl_str_mv |
bb2e9912251f8a8a2c2efecae38862af bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC) |
repository.mail.fl_str_mv |
biblicorjesu@unisagrado.edu.br||normalizacao@usc.br |
_version_ |
1800410548055048192 |