Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USC |
Texto Completo: | http://localhost:8080/tede/handle/tede/159 |
Resumo: | The objective of this work was to carry out an experimental study on the desnervation and the reinervation of the rat muscle soleus and also observe under morphologic and morphometric criteria, in which period after reinervation you can get a better standard of the related muscle. In order for that, twenty seven animals were used, aleatory divided in three groups of nine. In the group G1 (group zero day), it was carried out an incision between the tuber ischiatic and the trochanter bigger, where the sciatic nerve was dissected and sectioned in the height part of mid-thigh and immediately submitted to the technique of repair through the neurorhaphy terminus terminal. In the group G2 (group 10 days), the same procedure was carried out, but after the nervous section it was placed in the stump proximalis a polyethylene pipe of the blind fundus, fixed through wire 6-0. After ten days, the animals were again anesthetized to be submitted the reinervation through the described technique in group 1. In the group G3 (group 30 days), the same procedure was carried out, only differentiating the time and the wait of 30 days, to proceed the repair technique. The suture of the skin was made with the nylon wire 5-0. The animals had been sacrificed with superdosage of anesthetic, always after 10 days of repair to all groups. The removed muscle was first placed for some minutes in formaldehyde at 10% , to give form to the structure and after it was conditioned in appropriate container with formaldehyde 10% for twenty and four hours. After this period, the muscles were washed in running water for twenty and four hours and placed again in the same containers with alcohol 70%, until being taken to laboratorial procedures of routine. The gotten results had shown the success of the neurorrhaphy terminus terminalis in the recovery of the peripheral nervous without tissue loss, where in all groups the muscle fiber presented reinervation and the neuromuscular spindles, had kept with its normal intrafusal fiber numbers, understanding of 5 to 6 fiber in each one spindle. |
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Andreo, Jesus CarlosCPF:44798679887ANDREO, Jesus CarlosCPF:87974991968http://lattes.cnpq.br/2982398804664535Morgueti, Marcelo2015-09-14T13:13:05Z2008-10-222005-06-06MORGUETI, Marcelo. Changes in striated skeletal muscle fibers at different desnervadas and reinervadas periods. 2005. 47 f. Dissertação (Mestrado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2005.http://localhost:8080/tede/handle/tede/159The objective of this work was to carry out an experimental study on the desnervation and the reinervation of the rat muscle soleus and also observe under morphologic and morphometric criteria, in which period after reinervation you can get a better standard of the related muscle. In order for that, twenty seven animals were used, aleatory divided in three groups of nine. In the group G1 (group zero day), it was carried out an incision between the tuber ischiatic and the trochanter bigger, where the sciatic nerve was dissected and sectioned in the height part of mid-thigh and immediately submitted to the technique of repair through the neurorhaphy terminus terminal. In the group G2 (group 10 days), the same procedure was carried out, but after the nervous section it was placed in the stump proximalis a polyethylene pipe of the blind fundus, fixed through wire 6-0. After ten days, the animals were again anesthetized to be submitted the reinervation through the described technique in group 1. In the group G3 (group 30 days), the same procedure was carried out, only differentiating the time and the wait of 30 days, to proceed the repair technique. The suture of the skin was made with the nylon wire 5-0. The animals had been sacrificed with superdosage of anesthetic, always after 10 days of repair to all groups. The removed muscle was first placed for some minutes in formaldehyde at 10% , to give form to the structure and after it was conditioned in appropriate container with formaldehyde 10% for twenty and four hours. After this period, the muscles were washed in running water for twenty and four hours and placed again in the same containers with alcohol 70%, until being taken to laboratorial procedures of routine. The gotten results had shown the success of the neurorrhaphy terminus terminalis in the recovery of the peripheral nervous without tissue loss, where in all groups the muscle fiber presented reinervation and the neuromuscular spindles, had kept with its normal intrafusal fiber numbers, understanding of 5 to 6 fiber in each one spindle.O objetivo deste trabalho foi realizar um estudo experimental sobre a desnervação e a reinervação do músculo sóleo de rato e observar sob critérios morfológicos e morfométricos, em qual período pós reinervação pode se obter um melhor padrão do referido músculo. Para isso foram utilizados vinte e sete animais, divididos aleatoriamente em três grupos de nove cada um deles. No grupo G1(grupo zero dia), foi realizada uma incisão entre o túber isquiático e o trocanter maior, onde o nervo ciático foi dissecado e seccionado na altura do terço médio da coxa e imediatamente submetido à técnica de reparo através da neurorrafia término terminal. No grupo G2(grupo dez dias), o mesmo procedimento foi realizado, mas após o seccionamento do nervo, foi colocado no coto proximal um tubo de polietileno de fundo cego, fixado através de fio 6-0. Transcorridos dez dias os animais foram novamente anestesiados para serem submetidos à reinervação através da técnica descrita no grupo 1. No grupo G3 (grupo 30 dias), o mesmo procedimento foi realizado, somente diferenciando o tempo e espera de trinta dias, para se proceder a técnica de reparo. A sutura da pele foi realizada com o fio de nylon 5-0. Os animais foram sacrificados com superdosagem de anestésicos sempre decorridos 10 dias de reparo para todos os grupos. O músculo retirado foi primeiramente colocado por alguns minutos em formol tamponado a 10% para dar forma à estrutura e em seguida foi acondicionado em recipiente apropriado com formol 10% por vinte e quatro horas. Após este período, os músculos foram lavados em água corrente por mais vinte e quatro horas e novamente colocados nos mesmos recipientes contendo álcool a 70%, até serem levados para procedimentos laboratoriais de rotina. Os resultados obtidos mostraram o sucesso da técnica de neurorrafia término terminal na recuperação do nervo periférico sem perda tecidual, onde em todos os grupos, as fibras musculares se apresentaram reinervadas e os fusos neuromusculares se mantiveram com seus números de fibras intrafusais normais, compreendendo de 5 a 6 fibras em cada um fuso.Made available in DSpace on 2015-09-14T13:13:05Z (GMT). No. of bitstreams: 1 dissertacao_marcelo_morgueti.pdf: 1248286 bytes, checksum: 99ab0dd06e29d216ebfbeca4faacb777 (MD5) Previous issue date: 2005-06-06application/pdfporIASCJ - Universidade Sagrado CoraçãoBiologia OralUSCBRCiências da Saúde e BiológicasDESNERVAÇÃOREINERVAÇÃOMÚSCULO SÓLEONEURORRAFIA TÉRMINO TERMINALDESNERVATIONREINERVATIONMUSCLE SOLEUMNEURORHAPHY TERMINUS TERMINALISCIENCIAS DA SAUDE::ODONTOLOGIAAlterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodosChanges in striated skeletal muscle fibers at different desnervadas and reinervadas periodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8043334104093724546600600600-753428887677182963-2070498469879244349info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da USCinstname:Universidade do Sagrado Coração (USC)instacron:USCORIGINALdissertacao_marcelo_morgueti.pdfapplication/pdf1248286http://localhost:8080/tede/bitstream/tede/159/1/dissertacao_marcelo_morgueti.pdf99ab0dd06e29d216ebfbeca4faacb777MD51tede/1592015-10-16 17:53:19.034oai:localhost:tede/159Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.usc.br:8443/http://tede2.usc.br:8080/oai/requestbiblicorjesu@unisagrado.edu.br||normalizacao@usc.bropendoar:2015-10-16T20:53:19Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)false |
dc.title.por.fl_str_mv |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
dc.title.alternative.eng.fl_str_mv |
Changes in striated skeletal muscle fibers at different desnervadas and reinervadas periods |
title |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
spellingShingle |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos Morgueti, Marcelo DESNERVAÇÃO REINERVAÇÃO MÚSCULO SÓLEO NEURORRAFIA TÉRMINO TERMINAL DESNERVATION REINERVATION MUSCLE SOLEUM NEURORHAPHY TERMINUS TERMINALIS CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
title_full |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
title_fullStr |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
title_full_unstemmed |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
title_sort |
Alterações das fibras musculares estriadas esqueléticas desnervadas e reinervadas em diferentes períodos |
author |
Morgueti, Marcelo |
author_facet |
Morgueti, Marcelo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Andreo, Jesus Carlos |
dc.contributor.advisor1ID.fl_str_mv |
CPF:44798679887 |
dc.contributor.advisor1Lattes.fl_str_mv |
ANDREO, Jesus Carlos |
dc.contributor.authorID.fl_str_mv |
CPF:87974991968 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2982398804664535 |
dc.contributor.author.fl_str_mv |
Morgueti, Marcelo |
contributor_str_mv |
Andreo, Jesus Carlos |
dc.subject.por.fl_str_mv |
DESNERVAÇÃO REINERVAÇÃO MÚSCULO SÓLEO NEURORRAFIA TÉRMINO TERMINAL |
topic |
DESNERVAÇÃO REINERVAÇÃO MÚSCULO SÓLEO NEURORRAFIA TÉRMINO TERMINAL DESNERVATION REINERVATION MUSCLE SOLEUM NEURORHAPHY TERMINUS TERMINALIS CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
DESNERVATION REINERVATION MUSCLE SOLEUM NEURORHAPHY TERMINUS TERMINALIS |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The objective of this work was to carry out an experimental study on the desnervation and the reinervation of the rat muscle soleus and also observe under morphologic and morphometric criteria, in which period after reinervation you can get a better standard of the related muscle. In order for that, twenty seven animals were used, aleatory divided in three groups of nine. In the group G1 (group zero day), it was carried out an incision between the tuber ischiatic and the trochanter bigger, where the sciatic nerve was dissected and sectioned in the height part of mid-thigh and immediately submitted to the technique of repair through the neurorhaphy terminus terminal. In the group G2 (group 10 days), the same procedure was carried out, but after the nervous section it was placed in the stump proximalis a polyethylene pipe of the blind fundus, fixed through wire 6-0. After ten days, the animals were again anesthetized to be submitted the reinervation through the described technique in group 1. In the group G3 (group 30 days), the same procedure was carried out, only differentiating the time and the wait of 30 days, to proceed the repair technique. The suture of the skin was made with the nylon wire 5-0. The animals had been sacrificed with superdosage of anesthetic, always after 10 days of repair to all groups. The removed muscle was first placed for some minutes in formaldehyde at 10% , to give form to the structure and after it was conditioned in appropriate container with formaldehyde 10% for twenty and four hours. After this period, the muscles were washed in running water for twenty and four hours and placed again in the same containers with alcohol 70%, until being taken to laboratorial procedures of routine. The gotten results had shown the success of the neurorrhaphy terminus terminalis in the recovery of the peripheral nervous without tissue loss, where in all groups the muscle fiber presented reinervation and the neuromuscular spindles, had kept with its normal intrafusal fiber numbers, understanding of 5 to 6 fiber in each one spindle. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-06-06 |
dc.date.available.fl_str_mv |
2008-10-22 |
dc.date.accessioned.fl_str_mv |
2015-09-14T13:13:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MORGUETI, Marcelo. Changes in striated skeletal muscle fibers at different desnervadas and reinervadas periods. 2005. 47 f. Dissertação (Mestrado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2005. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/159 |
identifier_str_mv |
MORGUETI, Marcelo. Changes in striated skeletal muscle fibers at different desnervadas and reinervadas periods. 2005. 47 f. Dissertação (Mestrado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2005. |
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http://localhost:8080/tede/handle/tede/159 |
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por |
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por |
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600 600 600 |
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-2070498469879244349 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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IASCJ - Universidade Sagrado Coração |
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Biologia Oral |
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USC |
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BR |
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Ciências da Saúde e Biológicas |
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IASCJ - Universidade Sagrado Coração |
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