Avaliação da biocompatibilidade in vivo e da ação antimicrobiana de membranas para regeneração tecidual guiada impregnadas com extrato de Casearia sylvestris Sw

• Autor(a) principal: Gerzson, Alexandre da Silveira
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USC
• Texto Completo: http://localhost:8080/tede/handle/tede/87
• topic: REGENERAÇÃO ÓSSEA GUIADA; REGENERAÇÃO TECIDUAL GUIADA; MEMBRANAS REABSORVÍVEIS; BIOMATERIAIS; ANTIMICROBIANOS; GUIDED BONE REGENERATION; GUIDED TISSUE REGENERATION; RESORBABLE MEMBRANE; BIOMATERIALS; ANTIMICROBIAL; ODONTOLOGIA::CIRURGIA BUCO-MAXILO-FACIAL

The aim of this study was to evaluate the biocompatibility by histological analysis of resorbable membranes impregnated with extract of Casearia sylvestris Swart and check in vitro if impregnation with antimicrobial substance confers the same, and note the time of resorption of the four brands commercial use in research. We used 30 Wistar rats (Rattus norvegicus) from the animal house of the Sacred Heart University is divided into four experimental groups. The animals were killed at 7 days, 14 days and 30 days. The pieces were removed for histological analysis. The antimicrobial action of the substance used was analyzed by radial diffusion, against microorganisms Staphylococcus aureus, Enterococcus faecalis,Pseudomonas aeruginosas and Candida albicans. The results in 7 days had mainly membrane structure and the pattern of inflammatory infiltrate permeating the granulation tissue, with occasional multinucleated giant cells in a foreign body reaction in all groups. In only 14 days for Groups C and D remained membrane integrity and an inflammatory pattern similar to that of 7 days, while groups A and B showed fragmentation and resorption in some samples of advanced membrane, especially in group B with inflammatory infiltrate around and inside the membrane fragments with giant cells associated with fragments. At 30 days, only the D group showed membrane integrity in some samples, while the fragmented group C and groups A and B completely reabsorbed. The impregnation of the membranes did not appear to alter the behavior of resorption and inflammatory response, except at 14 days in group C, which reduced inflammation and 7 days in group B, which increased the inflammatory response.The antimicrobial test results showed only inhibition of S. aureus and C.albicans in CollaTape ®, showing no activity in other membranes, while that in the paper disc inhibition results occurred in three of the four tested strains, showing activity against Gram +.The findings of this study show that the lower inflammatory response was generated by the group D and the highest by the group B, while groups A and C in the middle. Group D showed the best results both in the inflammatory response, as in resorption. The absorption time of increasing sequence presented in the following order D-C-A-B. Slower and faster D to B. The Casearia sylvestris Sw biocompatibility has not increased the inflammatory response, but without adding an anti-inflammatory action to the membranes, except in group C at 14 days. The desire to add an antimicrobial resorbable membranes seems not to be possible by impregnation, because the membranes appear to be impervious to the substance.