Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USC |
Texto Completo: | http://localhost:8080/tede/handle/tede/147 |
Resumo: | Malignant neoplasms, popularly known as cancer, are characterized by autonomous and uncontrolled cell growth. The development of several human câncer is accompanied by high expression of the enzyme cyclooxygenase (COXs) responsible for the generation of various metabolites such as prostaglandins, and subsequent modulation of inflammatory chemical mediators. The aim of this study was to evaluate the effect of treatment with non-selective cyclooxygenase inhibitor on the development of solid Erhlich tumor, in periods of seven, fourteen and twenty-one days of treatment, correlating with tumor development, fibrosis and involvement of macrophages . After the evaluation period, the animals were euthanized, the tumor removed and routinely processed for HE staining for analysis of total area, area of necrosis and parenchyma. For evaluation of collagen type I, VEGF, and macrophages was used immunohistochemistry. The nonselective inhibition of cyclooxygenases resulted in significant reduction in total area to 14 days (14 days = 2.81 ± 0.85), an increase in dimension of necrotic area at 14 days of treatment (14 days = 2.25 ± 2.32 mm2), reduction in the areas parenchyma to 7:14 days of treatment (7days = 1.67 ± 1.71 mm2; 14dias = 1.70 ± 130mm2), significant reduction in collagen production after 14 days of treatment (14 days = 11.8 ± 6.2 mm2) and increased VEGF at 7 days of treatment (11.7 ± 7.0 mm2). Treatment did not affect the influx of macrophages. It can be concluded that the nonselective inhibition of cyclooxygenase was effective in controlling tumor growth in the intervening period of assessment and that in this period, the low production of collagen type I is involved in this contention, pointing to an important role of fibroblasts. However, no suggestion that macrophages are not involved, at least numerically, this containment of growth solid Ehrlich tumor. Additional studies are needed to clarify the involvement and the fact that the treatment in early stage stimulated the production of VEGF, important for the formation of a well-vascularized tissue for tumor implantation. |
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Okamoto, RobertaCPF:11739490894http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795623U4CPF:32125965895http://lattes.cnpq.br/0454706343336963Brito, André Cavichioli de2015-09-14T13:12:55Z2012-10-012012-02-28BRITO, André Cavichioli de. Growth of Ehrlich solid tumor front of nonselective inhibition of cyclooxygenase. 2012. 66 f. Tese (Doutorado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2012.http://localhost:8080/tede/handle/tede/147Malignant neoplasms, popularly known as cancer, are characterized by autonomous and uncontrolled cell growth. The development of several human câncer is accompanied by high expression of the enzyme cyclooxygenase (COXs) responsible for the generation of various metabolites such as prostaglandins, and subsequent modulation of inflammatory chemical mediators. The aim of this study was to evaluate the effect of treatment with non-selective cyclooxygenase inhibitor on the development of solid Erhlich tumor, in periods of seven, fourteen and twenty-one days of treatment, correlating with tumor development, fibrosis and involvement of macrophages . After the evaluation period, the animals were euthanized, the tumor removed and routinely processed for HE staining for analysis of total area, area of necrosis and parenchyma. For evaluation of collagen type I, VEGF, and macrophages was used immunohistochemistry. The nonselective inhibition of cyclooxygenases resulted in significant reduction in total area to 14 days (14 days = 2.81 ± 0.85), an increase in dimension of necrotic area at 14 days of treatment (14 days = 2.25 ± 2.32 mm2), reduction in the areas parenchyma to 7:14 days of treatment (7days = 1.67 ± 1.71 mm2; 14dias = 1.70 ± 130mm2), significant reduction in collagen production after 14 days of treatment (14 days = 11.8 ± 6.2 mm2) and increased VEGF at 7 days of treatment (11.7 ± 7.0 mm2). Treatment did not affect the influx of macrophages. It can be concluded that the nonselective inhibition of cyclooxygenase was effective in controlling tumor growth in the intervening period of assessment and that in this period, the low production of collagen type I is involved in this contention, pointing to an important role of fibroblasts. However, no suggestion that macrophages are not involved, at least numerically, this containment of growth solid Ehrlich tumor. Additional studies are needed to clarify the involvement and the fact that the treatment in early stage stimulated the production of VEGF, important for the formation of a well-vascularized tissue for tumor implantation.As neoplasias malignas popularmente conhecidas como câncer é caracterizado pelo crescimento celular desordenado e autônomo. O crescimento da maioria das neoplasias malignas é acompanhado da elevada expressão das enzimas cicloxigenases (COX s) responsáveis pela modulação de vários mediadores químicos. O objetivo desse estudo foi avaliar o efeito do tratamento com inibidor não seletivo de cicloxigenases sobre o desenvolvimento do tumor sólido de Erhlich, nos períodos de quatorze dias e vinte e um de tratamento, correlacionando com a evolução tumoral, fibrose e a participação de macrófagos. A metodologia de avaliação foi através da coloração H.E para área de total, área de necrose, para os demais paramento foi empregado a técnica de Imunoistoquimica marcação para macrófago, VEFG e fibras de colágenas tipo 1. A inibição não seletiva das ciclooxigenases resultou em redução significativa da área total aos 14 e 21 dias de tratamento (14 dias= 2,81±0,85; 21dias=5,58±1,32), um aumento nas dimensões das áreas de necrose aos 7 e 14 de tratamento (7 dias=0,66±0,24mm2; 14 dias=2,25±2,32mm2), redução nas ares de parênquima aos 7 e 14 de tratamento (7dias=1,67±1,71mm2; 14dias=1,70±130mm2), elevação significativa na produção de colágeno 7 e 21 de tratamento (7dias=18,8±9,4mm2; 21dias=13,0±7,9mm2) e VEGF um aumento em 7 dias de tratamento (11,7±7,0mm2). O Tratamento não afetou o influxo de macrófagos. Pode-se concluir que a inibição não seletiva de cicloxigenases foi efetiva em controlar o crescimento tumoral no período intermediário de avaliação e que, neste período, a baixa produção de colágeno tipo I está envolvida nesta contenção, apontando para um importante papel dos fibroblastos. Porém, há sugestão de que macrófagos não estejam envolvidos, pelo mesmo numericamente, nesta contenção do crescimento do tumor sólido de Ehrlich. Estudos adicionais são necessários para esclarecer a participação e o fato de que o tratamento em faze inicial estimulou a produção de VEGF, importante para a formação de um ambiente bem vascularizado para implantação tumoral.Made available in DSpace on 2015-09-14T13:12:55Z (GMT). No. of bitstreams: 1 tese_andre_cavichioli_de_brito.pdf: 8613023 bytes, checksum: 60da36a5659c3eb20ba8e57ba176315d (MD5) Previous issue date: 2012-02-28application/pdfporIASCJ - Universidade Sagrado CoraçãoBiologia OralUSCBRCiências da Saúde e BiológicasTUMOR SÓLIDO DE EHRLICHCICLOXIGENASESINDOMETACINAFIBRAS COLÁGENAS TIPO IFATOR DE CRESCIMENTO VASCULAR ENDOTELIALMACRÓFAGOSSOLID EHRLICH TUMORCYCLOOXYGENASEINDOMETHACINCOLLAGEN TYPE IVASCULAR ENDOTHELIAL GROWTH FACTORMACROPHAGESCIENCIAS BIOLOGICAS::FISIOLOGIACrescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenasesGrowth of Ehrlich solid tumor front of nonselective inhibition of cyclooxygenaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8043334104093724546600600600-7534288876771829637737708247419018223info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da USCinstname:Universidade do Sagrado Coração (USC)instacron:USCORIGINALtese_andre_cavichioli_de_brito.pdfapplication/pdf8613023http://localhost:8080/tede/bitstream/tede/147/1/tese_andre_cavichioli_de_brito.pdf60da36a5659c3eb20ba8e57ba176315dMD51tede/1472015-10-16 14:41:13.547oai:localhost:tede/147Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.usc.br:8443/http://tede2.usc.br:8080/oai/requestbiblicorjesu@unisagrado.edu.br||normalizacao@usc.bropendoar:2015-10-16T17:41:13Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)false |
dc.title.por.fl_str_mv |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
dc.title.alternative.eng.fl_str_mv |
Growth of Ehrlich solid tumor front of nonselective inhibition of cyclooxygenase |
title |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
spellingShingle |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases Brito, André Cavichioli de TUMOR SÓLIDO DE EHRLICH CICLOXIGENASES INDOMETACINA FIBRAS COLÁGENAS TIPO I FATOR DE CRESCIMENTO VASCULAR ENDOTELIAL MACRÓFAGOS SOLID EHRLICH TUMOR CYCLOOXYGENASE INDOMETHACIN COLLAGEN TYPE I VASCULAR ENDOTHELIAL GROWTH FACTOR MACROPHAGES CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
title_full |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
title_fullStr |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
title_full_unstemmed |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
title_sort |
Crescimento do tumor sólido de Ehrlich frente à inibição não seletiva das cicloxigenases |
author |
Brito, André Cavichioli de |
author_facet |
Brito, André Cavichioli de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Okamoto, Roberta |
dc.contributor.advisor1ID.fl_str_mv |
CPF:11739490894 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795623U4 |
dc.contributor.authorID.fl_str_mv |
CPF:32125965895 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0454706343336963 |
dc.contributor.author.fl_str_mv |
Brito, André Cavichioli de |
contributor_str_mv |
Okamoto, Roberta |
dc.subject.por.fl_str_mv |
TUMOR SÓLIDO DE EHRLICH CICLOXIGENASES INDOMETACINA FIBRAS COLÁGENAS TIPO I FATOR DE CRESCIMENTO VASCULAR ENDOTELIAL MACRÓFAGOS |
topic |
TUMOR SÓLIDO DE EHRLICH CICLOXIGENASES INDOMETACINA FIBRAS COLÁGENAS TIPO I FATOR DE CRESCIMENTO VASCULAR ENDOTELIAL MACRÓFAGOS SOLID EHRLICH TUMOR CYCLOOXYGENASE INDOMETHACIN COLLAGEN TYPE I VASCULAR ENDOTHELIAL GROWTH FACTOR MACROPHAGES CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
SOLID EHRLICH TUMOR CYCLOOXYGENASE INDOMETHACIN COLLAGEN TYPE I VASCULAR ENDOTHELIAL GROWTH FACTOR MACROPHAGES |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
Malignant neoplasms, popularly known as cancer, are characterized by autonomous and uncontrolled cell growth. The development of several human câncer is accompanied by high expression of the enzyme cyclooxygenase (COXs) responsible for the generation of various metabolites such as prostaglandins, and subsequent modulation of inflammatory chemical mediators. The aim of this study was to evaluate the effect of treatment with non-selective cyclooxygenase inhibitor on the development of solid Erhlich tumor, in periods of seven, fourteen and twenty-one days of treatment, correlating with tumor development, fibrosis and involvement of macrophages . After the evaluation period, the animals were euthanized, the tumor removed and routinely processed for HE staining for analysis of total area, area of necrosis and parenchyma. For evaluation of collagen type I, VEGF, and macrophages was used immunohistochemistry. The nonselective inhibition of cyclooxygenases resulted in significant reduction in total area to 14 days (14 days = 2.81 ± 0.85), an increase in dimension of necrotic area at 14 days of treatment (14 days = 2.25 ± 2.32 mm2), reduction in the areas parenchyma to 7:14 days of treatment (7days = 1.67 ± 1.71 mm2; 14dias = 1.70 ± 130mm2), significant reduction in collagen production after 14 days of treatment (14 days = 11.8 ± 6.2 mm2) and increased VEGF at 7 days of treatment (11.7 ± 7.0 mm2). Treatment did not affect the influx of macrophages. It can be concluded that the nonselective inhibition of cyclooxygenase was effective in controlling tumor growth in the intervening period of assessment and that in this period, the low production of collagen type I is involved in this contention, pointing to an important role of fibroblasts. However, no suggestion that macrophages are not involved, at least numerically, this containment of growth solid Ehrlich tumor. Additional studies are needed to clarify the involvement and the fact that the treatment in early stage stimulated the production of VEGF, important for the formation of a well-vascularized tissue for tumor implantation. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-10-01 |
dc.date.issued.fl_str_mv |
2012-02-28 |
dc.date.accessioned.fl_str_mv |
2015-09-14T13:12:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BRITO, André Cavichioli de. Growth of Ehrlich solid tumor front of nonselective inhibition of cyclooxygenase. 2012. 66 f. Tese (Doutorado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2012. |
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http://localhost:8080/tede/handle/tede/147 |
identifier_str_mv |
BRITO, André Cavichioli de. Growth of Ehrlich solid tumor front of nonselective inhibition of cyclooxygenase. 2012. 66 f. Tese (Doutorado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2012. |
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http://localhost:8080/tede/handle/tede/147 |
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por |
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por |
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600 600 600 |
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IASCJ - Universidade Sagrado Coração |
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Biologia Oral |
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USC |
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BR |
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Ciências da Saúde e Biológicas |
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IASCJ - Universidade Sagrado Coração |
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