Estudo da regeneração óssea guiada pela membrana de látex em associação com medula óssea

• Autor(a) principal: Holgado, Leandro de Andrade
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USC
• Texto Completo: http://localhost:8080/tede/handle/tede/125
• topic: MEDULA ÓSSEA; REGENERAÇÃO TECIDUAL GUIADA; MEMBRANAS OCLUSIVAS; LÁTEX; BONE MARROW; GUIDED TISSUE REGENERATION; OCCLUSIVE MEMBRANES; LATEX; CIENCIAS DA SAUDE::ODONTOLOGIA

Some methods have been studied as an attempt to minimize the time of bone repair, as well as less-invasive treatments in order to reduce the morbity associated to great reconstructive procedures. The transplant of bone marrow cells is an alternative to the use of autogenous bone graft. These cells have osteogenic potential due to the presence of mesenchymal stem cell that can differentiate in bone tissue cells. This work consists of the evaluation of the treatment with natural latex membrane, derivative of the Hevea brasiliensis, as an occlusive barrier, associated with autogenous bone marrow as a technique for stimulus of bone regeneration. Bilateral surgical bone defects with 10mm of diameter were made in rabbit skull. 30 animals, divided in 2 groups were used. The periods of study were 7 (n=6), 20 (n=12) and 60 (n=12) days. In the animals of Group 1, one of the defects was treated with latex membrane and the other with autogenous bone marrow associate to the latex membrane. In the animals of Group 2, one of the defects was treated with blood clot and the other with autogenous bone marrow. After the periods of study, the animals were euthanized and the results evaluated through microscopic, histomorphometric, radiographic and computerized tomography analysis. The photomicrografies of samples treated with bone marrow and membrane, 60 days post surgery, demonstrated a more advanced stage of bone regeneration in comparison to the other treatments of this study, presenting a bone bridge covering the extension of the defect. The quantitative analyses of newly formed bone through histomorphometry and computerized tomography reinforce this result, with results statistically significant (P<0.05) using ANOVA Tukey test for means comparisons.