Reação tecidual e performance como substituto ósseo de novos materiais compostos por fibroína

• Autor(a) principal: Nunes, Fernanda Parini
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USC
• Texto Completo: http://tede2.usc.br:8080/jspui/handle/tede/405
• topic: Biomaterial; Fibroína; Hidroxiapatita; Reação Tecidual; Enxerto Ósseo; Fibroin; Hydroxyapatite; Tissue Reaction; Bone Graft; CIENCIAS DA SAUDE::ODONTOLOGIA

For several dental procedures, the need for bone reconstruction to increase volume is frequent, in order to allow the future installation of osseointegrable implant or other procedures of oral rehabilitation. Autogenous bone replacement is not always available for this purpose. Due to the limitations of the autogenous bone, new biomaterials have been studied. Materials composed of fibroin are one of these alternatives, because fibroin is easily obtained as a waste from the silk industry and is considered a sustainable material, and at a very low cost. Thus the objective of this research was to propose the use of new material consisting of fibroin and hydroxyapatite, through the tissue reaction and efficacy of biomaterial. For this, 38 Wistar adult male rats were divided into 2 experiments. The study of the tissue reaction was carried out by the subcutaneous implantation of the back of 10 rats of the following materials: Fibroin, Fibroin associated with Hydroxyapatite (concentration of 100 and 200) and Commercial Hydroxyapatite, and also the control group which did not receive any material. The efficacy was analyzed by filling a surgically created defect in the skull cap with 5mm, with: (I) Fibroin associated with Hydroxyapatite (n = 10), (II) Autogenous bone substitute (n = 10), (III) Control (n = 08). After the periods of 3, 7 and 45 days, for the tissue reaction, and after 5 and 45 for the efficacy of biomaterial, the animals were submitted to euthanasia. The quantitative data were statistically evaluated by the Kruskal Wallis test and were considered statistically different when p <0.05. The results of the tissue reaction showed that there was a reduction in the number of inflammatory cells in relation to the 3 day period in all materials. In the period of 45 days, the number of cells found after the Hydroxyapatite implant was lower in relation to the other groups. Regarding to the performance as bone substitute, a smaller amount of bone tissue was observed in the Coagulum group after 45 days (p <0.05), and there was no difference between Fibroin and Autogenous (p> 0.05), indicating a good performance of the new biomaterial in the bone repair.