Reparo alveolar de ratos tratados com bisfosfonato sob ação de terapia antimicrobiana e laser de baixa potência

• Autor(a) principal: Moreschi, Eduardo
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USC
• Texto Completo: http://tede2.usc.br:8080/jspui/handle/tede/333
• topic: Antibiótico; Bisfosfonatos; Imuno-histoquímica; Laser de baixa potência; Ratos; Reparo alveolar; Reparo ósseo; Bisphosphonates; Bone repair; Immunohistochemistry; Low level laser; Socket healing; Rats; Antibiotic; ODONTOLOGIA::CIRURGIA BUCO-MAXILO-FACIAL

Osteonecrosis of the jaws induced by bisphosphonates is considered a relevant complication when there is the necessity of performing surgical procedures in oral cavity, since prevention and treatment are not well established. In this way, the aim of the present study is to analyze the effect of low level laser therapy (LLLT) on socket healing of animals treated with BFs. A total of 64 male adult Wistar rats were distributed into 8 groups, with a total of 8 animals per group (4 animals – 14 days and 4 animals – 28 days), according to the treatment: Group 1 – Control, animals treated with endovenous (EV) 0.9% saline solution, Group 2 – treated with EV 0.9% saline solution under LLLT, Group 3 - treated with EV 0.9% saline solution under antibiotic therapy, Group 4 - treated with EV 0.9% saline solution under LLLT and antibiotic therapy, Group 5 – treated with EV zoledronic acid, Group 6 - treated with EV zoledronic acid under LLLT, Group 7 - treated with EV zoledronic acid and antibiotic therapy, and Group 8 - treated with EV zoledronic acid under LLLT and antibiotic therapy. 0.035 mg/Kg of the BF were administered every two weeks. After four weeks from the beginning of the BF administration, the animals were submitted to upper first molars extractions. Sodium benzilpenicilin were administered 3 days before and 4 days after the extractions. The sockets of the animals from Groups 2, 4, 6, and 8 were irradiated with the low level laser (AlGaAs - 808nm, CW, 30mW, Ø ~ 0,028 cm2) with a total dose of 64 J/cm2 in one point of irradiation, perpendicularly to the socket during one minute, with an interval of 24 hours for three days, beginning in the immediate post-operatory time. After 14 and 28 days from the teeth extractions, the animals were euthanized with lethal dose of anesthetics for the retrieval of the specimens to be prepared and analyzed considering morphological microscopy and immunohistochemistry. The use of LLLT alone did not prevent the installation of osteonecrosis and did not benefit in a significant way the repair process when the administration of zoledronic acid (ZL). The use of antibiotic alone, as well as its association with LLLT best possible repair decreasing the inflammatory process