Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride

Detalhes bibliográficos
Autor(a) principal: Lee, Bin-Na
Data de Publicação: 2017
Outros Autores: Chun, Soo-Ji, Chang, Hoon-Sang, Hwang, Yun-Chan, Hwang, In-Nam, Oh, Won-Mann
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/142428
Resumo: Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2: 1% MC/CaCl2; Group 3: 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real­ time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (p
id USP-17_01d95d081ebbf00dc84d18a3b51a3dc5
oai_identifier_str oai:revistas.usp.br:article/142428
network_acronym_str USP-17
network_name_str Journal of applied oral science (Online)
repository_id_str
spelling Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chlorideFlowabilityMethylcelluloseMTAOsteogenic differentiation Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2: 1% MC/CaCl2; Group 3: 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real­ time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/14242810.1590/1678-7757-2017-0050Journal of Applied Oral Science; Vol. 25 No. 6 (2017); 680-688Journal of Applied Oral Science; Vol. 25 Núm. 6 (2017); 680-688Journal of Applied Oral Science; v. 25 n. 6 (2017); 680-6881678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/142428/137537Copyright (c) 2017 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessLee, Bin-NaChun, Soo-JiChang, Hoon-SangHwang, Yun-ChanHwang, In-NamOh, Won-Mann2018-01-18T16:07:28Zoai:revistas.usp.br:article/142428Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2018-01-18T16:07:28Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
title Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
spellingShingle Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
Lee, Bin-Na
Flowability
Methylcellulose
MTA
Osteogenic differentiation
title_short Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
title_full Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
title_fullStr Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
title_full_unstemmed Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
title_sort Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
author Lee, Bin-Na
author_facet Lee, Bin-Na
Chun, Soo-Ji
Chang, Hoon-Sang
Hwang, Yun-Chan
Hwang, In-Nam
Oh, Won-Mann
author_role author
author2 Chun, Soo-Ji
Chang, Hoon-Sang
Hwang, Yun-Chan
Hwang, In-Nam
Oh, Won-Mann
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lee, Bin-Na
Chun, Soo-Ji
Chang, Hoon-Sang
Hwang, Yun-Chan
Hwang, In-Nam
Oh, Won-Mann
dc.subject.por.fl_str_mv Flowability
Methylcellulose
MTA
Osteogenic differentiation
topic Flowability
Methylcellulose
MTA
Osteogenic differentiation
description Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2: 1% MC/CaCl2; Group 3: 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real­ time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (p
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/142428
10.1590/1678-7757-2017-0050
url https://www.revistas.usp.br/jaos/article/view/142428
identifier_str_mv 10.1590/1678-7757-2017-0050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/142428/137537
dc.rights.driver.fl_str_mv Copyright (c) 2017 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 25 No. 6 (2017); 680-688
Journal of Applied Oral Science; Vol. 25 Núm. 6 (2017); 680-688
Journal of Applied Oral Science; v. 25 n. 6 (2017); 680-688
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1800221680465870848

Registros relacionados