Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/216945 |
Resumo: | Oral candidiasis is a common fungal infection that affects the oral mucosa, and happens when Candida albicans interacts with bacteria in the oral microbiota, such as Streptococcus mutans, causing severe early childhood caries. C. albicans and S. mutans mixed biofilms are challenging to treat with conventional antimicrobial therapies, thus, new anti-infective drugs are required. Objective: This study aimed to test a drug delivery system based on chitosan microparticles loaded with geranium and lemongrass essential oils to inhibit C. albicans and S. mutans mixed biofilms. Methodology: Chitosan microparticles loaded with essential oils (CM-EOs) were obtained by spray-drying. Susceptibility of planktonic were performed according CLSI at 4 to 2,048 µg/mL. Mixed biofilms were incubated at 37ºC for 48 h and exposed to CM-EOs at 256 to 4,096 µg/mL. The antimicrobial effect was evaluated using the MTT assay, with biofilm architectural changes analyzed by scanning electron microscopy. RAW 264.7 cell was used to evaluate compound cytotoxicity. Results: CM-EOs had better planktonic activity against C. albicans than S. mutans. All samples reduced the metabolic activity of mixed C. albicans and S. mutans biofilms, with encapsulated oils showing better activity than raw chitosan or oils. The microparticles reduced the biofilm on the slides. The essential oils showed cytotoxic effects against RAW 264.7 cells, but encapsulation into chitosan microparticles decreased their toxicity. Conclusion: This study demonstrates that chitosan loaded with essential oils may provide an alternative method for treating diseases caused by C. albicans and S. mutans mixed biofilm, such as dental caries. |
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Journal of applied oral science (Online) |
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Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutansDrug effects on biofilmsDental plaqueCandida albicansChitosan analogs & derivativesEssential oilsOral candidiasis is a common fungal infection that affects the oral mucosa, and happens when Candida albicans interacts with bacteria in the oral microbiota, such as Streptococcus mutans, causing severe early childhood caries. C. albicans and S. mutans mixed biofilms are challenging to treat with conventional antimicrobial therapies, thus, new anti-infective drugs are required. Objective: This study aimed to test a drug delivery system based on chitosan microparticles loaded with geranium and lemongrass essential oils to inhibit C. albicans and S. mutans mixed biofilms. Methodology: Chitosan microparticles loaded with essential oils (CM-EOs) were obtained by spray-drying. Susceptibility of planktonic were performed according CLSI at 4 to 2,048 µg/mL. Mixed biofilms were incubated at 37ºC for 48 h and exposed to CM-EOs at 256 to 4,096 µg/mL. The antimicrobial effect was evaluated using the MTT assay, with biofilm architectural changes analyzed by scanning electron microscopy. RAW 264.7 cell was used to evaluate compound cytotoxicity. Results: CM-EOs had better planktonic activity against C. albicans than S. mutans. All samples reduced the metabolic activity of mixed C. albicans and S. mutans biofilms, with encapsulated oils showing better activity than raw chitosan or oils. The microparticles reduced the biofilm on the slides. The essential oils showed cytotoxic effects against RAW 264.7 cells, but encapsulation into chitosan microparticles decreased their toxicity. Conclusion: This study demonstrates that chitosan loaded with essential oils may provide an alternative method for treating diseases caused by C. albicans and S. mutans mixed biofilm, such as dental caries.Universidade de São Paulo. Faculdade de Odontologia de Bauru2023-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/21694510.1590/1678-7757-2023-0146Journal of Applied Oral Science; Vol. 31 (2023); e20230146Journal of Applied Oral Science; v. 31 (2023); e20230146Journal of Applied Oral Science; Vol. 31 (2023); e202301461678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/216945/198495Copyright (c) 2023 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGarcia, Lana Glerieide Silva Rocha, Maria Gleiciane daFreire, Rosemayre SouzaNunes, Paulo Iury GomesNunes, João Victor SerraFernandes, Mirele RodriguesSidrim, José Júlio CostaSantos, Flavia AlmeidaRocha, Marcos Fábio GadelhaRodrigues, Lidiany Karla AzevedoVieira, Rodrigo SilveiraBrilhante, Raimunda Sâmia Nogueira2024-02-07T17:41:05Zoai:revistas.usp.br:article/216945Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2024-02-07T17:41:05Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
title |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
spellingShingle |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans Garcia, Lana Glerieide Silva Drug effects on biofilms Dental plaque Candida albicans Chitosan analogs & derivatives Essential oils |
title_short |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
title_full |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
title_fullStr |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
title_full_unstemmed |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
title_sort |
Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans |
author |
Garcia, Lana Glerieide Silva |
author_facet |
Garcia, Lana Glerieide Silva Rocha, Maria Gleiciane da Freire, Rosemayre Souza Nunes, Paulo Iury Gomes Nunes, João Victor Serra Fernandes, Mirele Rodrigues Sidrim, José Júlio Costa Santos, Flavia Almeida Rocha, Marcos Fábio Gadelha Rodrigues, Lidiany Karla Azevedo Vieira, Rodrigo Silveira Brilhante, Raimunda Sâmia Nogueira |
author_role |
author |
author2 |
Rocha, Maria Gleiciane da Freire, Rosemayre Souza Nunes, Paulo Iury Gomes Nunes, João Victor Serra Fernandes, Mirele Rodrigues Sidrim, José Júlio Costa Santos, Flavia Almeida Rocha, Marcos Fábio Gadelha Rodrigues, Lidiany Karla Azevedo Vieira, Rodrigo Silveira Brilhante, Raimunda Sâmia Nogueira |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Garcia, Lana Glerieide Silva Rocha, Maria Gleiciane da Freire, Rosemayre Souza Nunes, Paulo Iury Gomes Nunes, João Victor Serra Fernandes, Mirele Rodrigues Sidrim, José Júlio Costa Santos, Flavia Almeida Rocha, Marcos Fábio Gadelha Rodrigues, Lidiany Karla Azevedo Vieira, Rodrigo Silveira Brilhante, Raimunda Sâmia Nogueira |
dc.subject.por.fl_str_mv |
Drug effects on biofilms Dental plaque Candida albicans Chitosan analogs & derivatives Essential oils |
topic |
Drug effects on biofilms Dental plaque Candida albicans Chitosan analogs & derivatives Essential oils |
description |
Oral candidiasis is a common fungal infection that affects the oral mucosa, and happens when Candida albicans interacts with bacteria in the oral microbiota, such as Streptococcus mutans, causing severe early childhood caries. C. albicans and S. mutans mixed biofilms are challenging to treat with conventional antimicrobial therapies, thus, new anti-infective drugs are required. Objective: This study aimed to test a drug delivery system based on chitosan microparticles loaded with geranium and lemongrass essential oils to inhibit C. albicans and S. mutans mixed biofilms. Methodology: Chitosan microparticles loaded with essential oils (CM-EOs) were obtained by spray-drying. Susceptibility of planktonic were performed according CLSI at 4 to 2,048 µg/mL. Mixed biofilms were incubated at 37ºC for 48 h and exposed to CM-EOs at 256 to 4,096 µg/mL. The antimicrobial effect was evaluated using the MTT assay, with biofilm architectural changes analyzed by scanning electron microscopy. RAW 264.7 cell was used to evaluate compound cytotoxicity. Results: CM-EOs had better planktonic activity against C. albicans than S. mutans. All samples reduced the metabolic activity of mixed C. albicans and S. mutans biofilms, with encapsulated oils showing better activity than raw chitosan or oils. The microparticles reduced the biofilm on the slides. The essential oils showed cytotoxic effects against RAW 264.7 cells, but encapsulation into chitosan microparticles decreased their toxicity. Conclusion: This study demonstrates that chitosan loaded with essential oils may provide an alternative method for treating diseases caused by C. albicans and S. mutans mixed biofilm, such as dental caries. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/216945 10.1590/1678-7757-2023-0146 |
url |
https://www.revistas.usp.br/jaos/article/view/216945 |
identifier_str_mv |
10.1590/1678-7757-2023-0146 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/216945/198495 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 31 (2023); e20230146 Journal of Applied Oral Science; v. 31 (2023); e20230146 Journal of Applied Oral Science; Vol. 31 (2023); e20230146 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221670359695360 |