Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/202618 |
Resumo: | Objective: This study evaluated the expression of pro-inflammatory (IL-1β, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-β) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. Results: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-β and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. Conclusions: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-β were related to some clinical and CBCT data. |
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Journal of applied oral science (Online) |
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Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitisPeriapical periodontitisPeriapical granulomaRadicular cystCytokinesObjective: This study evaluated the expression of pro-inflammatory (IL-1β, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-β) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. Results: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-β and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. Conclusions: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-β were related to some clinical and CBCT data.Universidade de São Paulo. Faculdade de Odontologia de Bauru2022-09-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/20261810.1590/1678-7757-2017-0455 Journal of Applied Oral Science; Vol. 26 (2018); e20170455Journal of Applied Oral Science; Vol. 26 (2018); e20170455Journal of Applied Oral Science; v. 26 (2018); e201704551678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/202618/186704Copyright (c) 2022 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDessaune Neto, NiltonPorpino, Mariana Teixeira ManeschyAntunes, Henrique dos SantosRodrigues, Renata Costa ValRon Perez, AlejandroPires, Fábio RamôaSiqueira Júnior, José FreitasArmada, Luciana 2022-09-20T18:32:43Zoai:revistas.usp.br:article/202618Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2022-09-20T18:32:43Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
title |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
spellingShingle |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis Dessaune Neto, Nilton Periapical periodontitis Periapical granuloma Radicular cyst Cytokines |
title_short |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
title_full |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
title_fullStr |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
title_full_unstemmed |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
title_sort |
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis |
author |
Dessaune Neto, Nilton |
author_facet |
Dessaune Neto, Nilton Porpino, Mariana Teixeira Maneschy Antunes, Henrique dos Santos Rodrigues, Renata Costa Val Ron Perez, Alejandro Pires, Fábio Ramôa Siqueira Júnior, José Freitas Armada, Luciana |
author_role |
author |
author2 |
Porpino, Mariana Teixeira Maneschy Antunes, Henrique dos Santos Rodrigues, Renata Costa Val Ron Perez, Alejandro Pires, Fábio Ramôa Siqueira Júnior, José Freitas Armada, Luciana |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Dessaune Neto, Nilton Porpino, Mariana Teixeira Maneschy Antunes, Henrique dos Santos Rodrigues, Renata Costa Val Ron Perez, Alejandro Pires, Fábio Ramôa Siqueira Júnior, José Freitas Armada, Luciana |
dc.subject.por.fl_str_mv |
Periapical periodontitis Periapical granuloma Radicular cyst Cytokines |
topic |
Periapical periodontitis Periapical granuloma Radicular cyst Cytokines |
description |
Objective: This study evaluated the expression of pro-inflammatory (IL-1β, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-β) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. Results: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-β and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. Conclusions: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-β were related to some clinical and CBCT data. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/202618 10.1590/1678-7757-2017-0455 |
url |
https://www.revistas.usp.br/jaos/article/view/202618 |
identifier_str_mv |
10.1590/1678-7757-2017-0455 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/202618/186704 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 26 (2018); e20170455 Journal of Applied Oral Science; Vol. 26 (2018); e20170455 Journal of Applied Oral Science; v. 26 (2018); e20170455 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683242500096 |