Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis

Detalhes bibliográficos
Autor(a) principal: Dessaune Neto, Nilton
Data de Publicação: 2022
Outros Autores: Porpino, Mariana Teixeira Maneschy, Antunes, Henrique dos Santos, Rodrigues, Renata Costa Val, Ron Perez, Alejandro, Pires, Fábio Ramôa, Siqueira Júnior, José Freitas, Armada, Luciana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/202618
Resumo: Objective: This study evaluated the expression of pro-inflammatory (IL-1β, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-β) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. Results: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-β and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. Conclusions: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-β were related to some clinical and CBCT data.
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spelling Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitisPeriapical periodontitisPeriapical granulomaRadicular cystCytokinesObjective: This study evaluated the expression of pro-inflammatory (IL-1β, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-β) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. Results: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-β and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. Conclusions: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-β were related to some clinical and CBCT data.Universidade de São Paulo. Faculdade de Odontologia de Bauru2022-09-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/20261810.1590/1678-7757-2017-0455 Journal of Applied Oral Science; Vol. 26 (2018); e20170455Journal of Applied Oral Science; Vol. 26 (2018); e20170455Journal of Applied Oral Science; v. 26 (2018); e201704551678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/202618/186704Copyright (c) 2022 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDessaune Neto, NiltonPorpino, Mariana Teixeira ManeschyAntunes, Henrique dos SantosRodrigues, Renata Costa ValRon Perez, AlejandroPires, Fábio RamôaSiqueira Júnior, José FreitasArmada, Luciana 2022-09-20T18:32:43Zoai:revistas.usp.br:article/202618Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2022-09-20T18:32:43Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
title Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
spellingShingle Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
Dessaune Neto, Nilton
Periapical periodontitis
Periapical granuloma
Radicular cyst
Cytokines
title_short Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
title_full Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
title_fullStr Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
title_full_unstemmed Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
title_sort Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
author Dessaune Neto, Nilton
author_facet Dessaune Neto, Nilton
Porpino, Mariana Teixeira Maneschy
Antunes, Henrique dos Santos
Rodrigues, Renata Costa Val
Ron Perez, Alejandro
Pires, Fábio Ramôa
Siqueira Júnior, José Freitas
Armada, Luciana
author_role author
author2 Porpino, Mariana Teixeira Maneschy
Antunes, Henrique dos Santos
Rodrigues, Renata Costa Val
Ron Perez, Alejandro
Pires, Fábio Ramôa
Siqueira Júnior, José Freitas
Armada, Luciana
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dessaune Neto, Nilton
Porpino, Mariana Teixeira Maneschy
Antunes, Henrique dos Santos
Rodrigues, Renata Costa Val
Ron Perez, Alejandro
Pires, Fábio Ramôa
Siqueira Júnior, José Freitas
Armada, Luciana
dc.subject.por.fl_str_mv Periapical periodontitis
Periapical granuloma
Radicular cyst
Cytokines
topic Periapical periodontitis
Periapical granuloma
Radicular cyst
Cytokines
description Objective: This study evaluated the expression of pro-inflammatory (IL-1β, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-β) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. Results: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-β and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. Conclusions: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-β were related to some clinical and CBCT data.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/202618
10.1590/1678-7757-2017-0455
url https://www.revistas.usp.br/jaos/article/view/202618
identifier_str_mv 10.1590/1678-7757-2017-0455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/202618/186704
dc.rights.driver.fl_str_mv Copyright (c) 2022 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Journal of Applied Oral Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 26 (2018); e20170455
Journal of Applied Oral Science; Vol. 26 (2018); e20170455
Journal of Applied Oral Science; v. 26 (2018); e20170455
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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