Clinical attachment loss and molecular profile of inflamed sites before treatment

Detalhes bibliográficos
Autor(a) principal: Borges,Cristine D'Almeida
Data de Publicação: 2019
Outros Autores: Ricoldi,Milla Sprone, Messora,Michel Reis, Palioto,Daniela Bazan, Souza,Sérgio Luís Scombatti de, Novaes Júnior,Arthur Belém, Taba Jr,Mario
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100477
Resumo: Abstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.
id USP-17_1e2b81c051acde1c4b4a833c212b2f4e
oai_identifier_str oai:scielo:S1678-77572019000100477
network_acronym_str USP-17
network_name_str Journal of applied oral science (Online)
repository_id_str
spelling Clinical attachment loss and molecular profile of inflamed sites before treatmentPeriodontal attachment lossBiological markersGingival crevicular fluidBiopsyGene expressionAbstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.Faculdade De Odontologia De Bauru - USP2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100477Journal of Applied Oral Science v.27 2019reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2018-0671info:eu-repo/semantics/openAccessBorges,Cristine D'AlmeidaRicoldi,Milla SproneMessora,Michel ReisPalioto,Daniela BazanSouza,Sérgio Luís Scombatti deNovaes Júnior,Arthur BelémTaba Jr,Marioeng2019-09-04T00:00:00Zoai:scielo:S1678-77572019000100477Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2019-09-04T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Clinical attachment loss and molecular profile of inflamed sites before treatment
title Clinical attachment loss and molecular profile of inflamed sites before treatment
spellingShingle Clinical attachment loss and molecular profile of inflamed sites before treatment
Borges,Cristine D'Almeida
Periodontal attachment loss
Biological markers
Gingival crevicular fluid
Biopsy
Gene expression
title_short Clinical attachment loss and molecular profile of inflamed sites before treatment
title_full Clinical attachment loss and molecular profile of inflamed sites before treatment
title_fullStr Clinical attachment loss and molecular profile of inflamed sites before treatment
title_full_unstemmed Clinical attachment loss and molecular profile of inflamed sites before treatment
title_sort Clinical attachment loss and molecular profile of inflamed sites before treatment
author Borges,Cristine D'Almeida
author_facet Borges,Cristine D'Almeida
Ricoldi,Milla Sprone
Messora,Michel Reis
Palioto,Daniela Bazan
Souza,Sérgio Luís Scombatti de
Novaes Júnior,Arthur Belém
Taba Jr,Mario
author_role author
author2 Ricoldi,Milla Sprone
Messora,Michel Reis
Palioto,Daniela Bazan
Souza,Sérgio Luís Scombatti de
Novaes Júnior,Arthur Belém
Taba Jr,Mario
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Borges,Cristine D'Almeida
Ricoldi,Milla Sprone
Messora,Michel Reis
Palioto,Daniela Bazan
Souza,Sérgio Luís Scombatti de
Novaes Júnior,Arthur Belém
Taba Jr,Mario
dc.subject.por.fl_str_mv Periodontal attachment loss
Biological markers
Gingival crevicular fluid
Biopsy
Gene expression
topic Periodontal attachment loss
Biological markers
Gingival crevicular fluid
Biopsy
Gene expression
description Abstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100477
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100477
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-7757-2018-0671
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.27 2019
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
_version_ 1748936440418402304